scholarly journals Evaluation of Peroxyacetic Acid (PAA) for Controlling Bacterial Soft Rot of some Vegetable Fruits and Potato Tubers Caused by Erwinia carotovora subsp. carotovora

2017 ◽  
Vol 45 (2) ◽  
pp. 79-91
Author(s):  
Anwar Galal
Keyword(s):  
Erwinia Carotovora ◽  
Soft Rot ◽  
Bacterial Soft Rot ◽  
Peroxyacetic Acid ◽  
Potato Tubers

Use of 1,4-naphthoquinones for control of Erwinia carotovora

2004 ◽  
Vol 50 (11) ◽  
pp. 951-956 ◽  
Author(s):  
Luis F.C Medina ◽  
Valter Stefani ◽  
Adriano Brandelli
Keyword(s):  
Antimicrobial Effect ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Sodium Ascorbate ◽  
Bacterial Suspension ◽  
Oxygen Species ◽  
Potato Tubers ◽  
Phytopathogenic Bacteria ◽  
Minimal Inhibitory Concentrations

The antimicrobial effect of 5 naphthoquinones was tested against the phytopathogenic bacteria Erwinia carotovora. Disk diffusion tests and determination of minimal inhibitory concentrations (MIC) indicate that the compound naphthazarin (NTZ) has the best antibacterial activity among the naphthoquinones tested. Studies on the mode of action indicate the effect of NTZ was bactericidal at 10 µg/mL. When cultivation was done in the presence of sodium ascorbate, the restoration of E. carotovora growth was observed with 3 µg/mL NTZ, but not when a 10 µg/mL dose was used. The incubation of NTZ with bacterial suspension of E. carotovora resulted in important changes in the absorption spectra of this naphthoquinone, indicating that a redox reaction takes place. These results may suggest that NTZ induces an increase of reactive oxygen species that are toxic to the cell. The compound NTZ was also effective in preventing E. carotovora growth on potato tubers, inhibiting the soft rot development at a concentration of 2 mg/mL.Key words: antimicrobial, naphthazarin, phytopathogen, potato, 5,8-dihydroxy-1,4-naphthoquinone.

Bacteriocin-like substance inhibits potato soft rot caused by Erwinia carotovora

2006 ◽  
Vol 52 (6) ◽  
pp. 533-539 ◽  
Author(s):  
Florencia Cladera-Olivera ◽  
Geruza R Caron ◽  
Amanda S Motta ◽  
André A Souto ◽  
Adriano Brandelli
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Bacillus Licheniformis ◽  
Membrane Lipids ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Bactericidal Effect ◽  
Potato Tubers ◽  
Transmission Electron ◽  
Bacterial Surfaces

Soft rot is a major problem encountered in potatoes during postharvest storage. The soft rot bacterium Erwinia carotovora was inhibited by a novel bacteriocin-like substance (BLS) produced by Bacillus licheniformis P40. The BLS caused a bactericidal effect on E. carotovora cells at 30 µg mL–1. Transmission electron microscopy showed that BLS-treated cells presented wrinkled bacterial surfaces and shrinkage of the whole cell, indicating plasmolysis. Erwinia carotovora cells treated with BLS were analyzed by FTIR showing differences in the 1390 cm–1 and 1250–1220 cm–1 bands, corresponding to assignments of membrane lipids. BLS was effective in preventing E. carotovora spoilage on potato tubers, reducing the symptoms of soft rot at 240 µg mL–1 and higher concentrations. Soft rot development was completely blocked at 3.7 mg mL–1. This BLS showed potential to protect potato tubers during storage. Key words: bacteriocin, plant pathogen, potato, soft rot, storage.

scholarly journals Assessing Susceptibility of Carrot Roots to Bacterial Soft Rot

HortScience ◽  
1992 ◽  
Vol 27 (9) ◽  
pp. 1020-1022 ◽  
Author(s):  
Barbara Michalik ◽  
Philipp W. Simon ◽  
Warren H. Gabelman
Keyword(s):  
Screening Method ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Bacterial Suspension ◽  
Bacterial Soft Rot ◽  
Severe Damage ◽  
Cross Sectional ◽  
Severity Of Disease ◽  
Disease Damage ◽  
Consistent Response

Four methods for screening carrot (Daucus carota L.) germplasm for resistance to bacterial soft rot were compared. There were differences in resistance among strains, with most severe damage caused by Erwinia carotovora pv. carotovora SR 394 (L.R. Jones) Holland and Erwinia carotovora pv. atroseptica SR 159 (van Hall) Jennison. Inoculation of cross-sectional root slices with bacteria applied in suspension-soaked paper disks produced the most consistent response. The severity of disease damage was proportional to bacterial suspension concentration. With the development of a standard screening method, it may be possible for breeders to breed carrots with reduced susceptibility to soft rot.

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