scholarly journals Isolation and Protein Characterization of Lindane Degrading Root Epiphytic Bacterium Arthrobacter sp. T16 from Typha latifolia

2018 ◽  
Vol 10 (4) ◽  
pp. 559-566 ◽  
Author(s):  
Tanvi SINGH ◽  
Dileep K. SINGH
Keyword(s):  
Average Rate ◽  
Elongation Factor ◽  
Typha Latifolia ◽  
Protein Characterization ◽  
Chloride Ions ◽  
Gas Liquid Chromatography ◽  
Epiphytic Bacteria ◽  
Enrichment Technique ◽  
Amino Acid Binding ◽  
Protein Elongation

Lindane, extensively used as pesticide, causes severe environmental hazard and is a threat to the humanity. The present study aims to assess the capability and mechanism of root epiphytic bacteria of wetland plant Typha latifolia to degrade lindane. Isolation of lindane degrading root epiphytic bacteria was done by standard enrichment technique and lindane degradation analysis was done using Gas Liquid Chromatography. Bacterial strain Arthrobacter sp. T16 was isolated and identified, which showed maximum degradation of 71.2 ± 1.3% of 50 mg l-1 lindane. Lindane biodegradation was accompanied with decrease in pH, increase in chloride ions concentration of culture medium and a positive dechlorination assay. Biodegradation potential of Arthrobacter sp. T16 was also studied at different lindane concentrations. Maximum degradation was observed at 10 mg l-1 lindane followed by 50 mg l-1 and 100 mg l-1 lindane. Lindane biodegradation kinetics study inferred that the average rate of lindane degradation increased with increase in lindane concentration. Lindane induced proteins in Arthrobacter sp. T16 were studied by SDS-PAGE. Distinctive polypeptides came into view in the presence of lindane and were identified as putative ABC transporter periplasmic amino acid-binding protein, elongation factor Tu and trifunctional transcriptional regulator/proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase, each expressed due to lindane stress. This study specifies the potential of phytoremediation in controlling the environmental contamination problem with the help of indigenous organisms present in roots of plants.

Protein elongation factor EEF1A2 is a putative oncogene in ovarian cancer

10.1038/ng904 ◽  
2002 ◽  
Vol 31 (3) ◽  
pp. 301-305 ◽  
Author(s):  
Nisha Anand ◽  
Sabita Murthy ◽  
Gudrun Amann ◽  
Meredith Wernick ◽  
Lisa A. Porter ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Elongation Factor ◽  
Protein Elongation

scholarly journals Transcription and translation in an RNA world

2006 ◽  
Vol 361 (1474) ◽  
pp. 1751-1760 ◽  
Author(s):  
William R Taylor
Keyword(s):  
Amino Acid ◽  
Active Site ◽  
Rna World ◽  
Large Subunit ◽  
Trna Synthetase ◽  
Nucleoside Triphosphate ◽  
Amino Acid Binding ◽  
World Hypothesis ◽  
Protein Elongation ◽  
Rna World Hypothesis

The RNA world hypothesis requires a ribozyme that was an RNA-directed RNA polymerase (ribopolymerase). If such a replicase makes a reverse complementary copy of any sequence (including itself), in a simple RNA world, there is no mechanism to prevent self-hybridization. It is proposed that this can be avoided through the synthesis of a parallel complementary copy. The logical consequences of this are pursued and developed in a computer simulation, where the behaviour of the parallel copy is compared to the conventional reverse complementary copy. It is found that the parallel copy is more efficient at higher temperatures (up to 90°C). A model for the ribopolymerase, based on the core of the large subunit (LSU) of the ribosome, is described. The geometry of a potential active site for this ribopolymerase suggests that it contained a cavity (now occupied by the aminoacyl-tRNA) and that an amino acid binding in this might have ‘poisoned’ the ribopolymerase by cross-reacting with the nucleoside-triphosphate before polymerization could occur. Based on a similarity to the active site components of the class-I tRNA synthetase enzymes, it is proposed that the amino acid could become attached to the nascent RNA transcript producing a variety of aminoacylated tRNA-like products. Using base-pairing interactions, some of these molecules might cross-link two ribopolymerases, giving rise to a precursor of the modern ribosome. A hybrid dimer, half polymerase and half proto-ribosome, could account for mRNA translocation before the advent of protein elongation factors.

The possible interaction of CDA14 and protein elongation factor 1α

2008 ◽  
Vol 1784 (2) ◽  
pp. 312-318 ◽  
Author(s):  
Ying-Fang Yang ◽  
Min-Yuan Chou ◽  
Chia-Yu Fan ◽  
Sung-Fang Chen ◽  
Ping-Chiang Lyu ◽  
...  
Keyword(s):  
Elongation Factor ◽  
Elongation Factor 1Α ◽  
Protein Elongation

Expression of protein elongation factor eEF1A2 predicts favorable outcome in breast cancer

2006 ◽  
Vol 102 (1) ◽  
pp. 31-41 ◽  
Author(s):  
Geeta Kulkarni ◽  
Dmitry A. Turbin ◽  
Anahita Amiri ◽  
Sujeeve Jeganathan ◽  
Miguel A. Andrade-Navarro ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Elongation Factor ◽  
Favorable Outcome ◽  
Protein Elongation

scholarly journals Plitidepsin, an inhibitor of the cell elongation factor eEF1a, and molnupiravir an analogue of the ribonucleoside cytidine, two new chemical compounds with intense activity against SARS-CoV-2

2021 ◽  
Author(s):  
Jordi Reina ◽  
Keyword(s):  
Elongation Factor ◽  
Chemical Compounds ◽  
Cellular Protein ◽  
Lethal Mutagenesis ◽  
Good Target ◽  
Therapeutic Possibility ◽  
Intense Activity ◽  
Protein Elongation ◽  
Natural Inhibitors ◽  
Replicative Cycle

The knowledge of the replicative cycle of SARS-CoV-2 and its interactions with cellular proteins has opened a new therapeutic possibility based on blocking those essential for the virus. The cellular protein elongation factor eEF1A could be a good target. Among its natural inhibitors are didemnins and their related chemical compounds such as plitidepsin. In human cell culture, this compound is capable of inhibiting the virus with a potency 27,5 times that of remdesivir. It must be administered intravenously. Of the ribonucleoside analogues, molnupiravir (MK-4483/EIDD-2801) (hydroxy-cytidine) determines a lethal mutagenesis on SARS-CoV-2. In animals, after oral administration, the pulmonary viral load decreases 25,000 times and when administered as prophylaxis, approximately 100,000 times. It prevents the transmission of the virus and eliminates its presence in the oropharynx. Both chemicals have started Phase I / II human clinical trials

scholarly journals COMPARATIVE ANALYSIS OF FATTY ACIDS COMPOSITION OF COASTAL-WATER TYPHA LATIFOLIA, SUBMERGED CERATOPHYLLUM DEMERSUM AND WATER FORM VERONICA ANAGALLIS-AQUATICA FROM WATER BODIES OF BAIKALIAN REGION

2019 ◽  
pp. 119-128
Author(s):  
Kuz'ma Anatol'yevich Kirichenko ◽  
Tamara Pavlovna Pobezhimova ◽  
Sergey Grigor'yevich Kazanovsky ◽  
Natal'ya Aleksandrovna Sokolova ◽  
Elizaveta Sergeevna Kondrateva ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Coastal Water ◽  
Ecological Status ◽  
Typha Latifolia ◽  
Water Bodies ◽  
Ceratophyllum Demersum ◽  
Gas Liquid Chromatography

The fatty acid comparative essay of high aquatic plants: Typha latifolia L., Ceratophyllum demersum L. and Veronica anagallis-aquatica L. from water bodies of Baikalian region was carried out. For the first time this biochemical parameter of water form V. anagallis-aquatica was compared with that of coastal-water T. latifolia and submerged C. demersum. The fifteen individual molecules of fatty acids with a chain length from 12 to 22 carbon atoms have been identified by the method of gas liquid chromatography – mass spectrometry. The fatty acid composition of investigated species was different. Among the fatty acids saturated and unsaturated molecules was found, the most distributed fatty acids was saturated palmitic (С16:0), steric (С18:0) and unsaturated linoleic (С18:2 w6), α-linolenic (С18:3 w3). In total, the C16 and C18 acids in studied species accounted for 98%. Differences in the composition of fatty acids of the studied species are discussed from the standpoint of the characteristics of fatty acid metabolism depending on the habitat of plants and are considered as one of the mechanisms of their adaptation to late vegetation. Studies of the fatty acid composition of aquatic organisms contribute to the overall strategy of plant adaptation to changing environmental conditions and are important for the ecological and biochemical monitoring of the ecological status of water bodies.

scholarly journals F-actin sequesters elongation factor 1alpha from interaction with aminoacyl-tRNA in a pH-dependent reaction.

1996 ◽  
Vol 135 (4) ◽  
pp. 953-963 ◽  
Author(s):  
G Liu ◽  
J Tang ◽  
B T Edmonds ◽  
J Murray ◽  
S Levin ◽  
...  
Keyword(s):  
Ph Dependence ◽  
Elongation Factor ◽  
Ph Sensitive ◽  
Actin Binding ◽  
Local Concentration ◽  
Competition Binding ◽  
Translational Apparatus ◽  
Protein Elongation ◽  
Trna Binding

The machinery of eukaryotic protein synthesis is found in association with the actin cytoskeleton. A major component of this translational apparatus, which is involved in the shuttling of aa-tRNA, is the actin-binding protein elongation factor 1alpha (EF-1alpha). To investigate the consequences for translation of the interaction of EF-1alpha with F-actin, we have studied the effect of F-actin on the ability of EF-1alpha to bind to aa-tRNA. We demonstrate that binding of EF-1alpha:GTP to aa-tRNA is not pH sensitive with a constant binding affinity of approximately 0.2 microM over the physiological range of pH. However, the sharp pH dependence of binding of EF-1alpha to F-actin is sufficient to shift the binding of EF-1alpha from F-actin to aa-tRNA as pH increases. The ability of EF-1alpha to bind either F-actin or aa-tRNA in competition binding experiments is also consistent with the observation that EF-1alpha's binding to F-actin and aa-tRNA is mutually exclusive. Two pH-sensitive actin-binding sequences in EF-1alpha are identified and are predicted to overlap with the aa-tRNA-binding sites. Our results suggest that pH-regulated recruitment and release of EF-1alpha from actin filaments in vivo will supply a high local concentration of EF-1alpha to facilitate polypeptide elongation by the F-actin-associated translational apparatus.

scholarly journals Comparative mRNA Expression of eEF1A Isoforms and a PI3K/Akt/mTOR Pathway in a Cellular Model of Parkinson’s Disease

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Kawinthra Khwanraj ◽  
Suriyat Madlah ◽  
Khwanthana Grataitong ◽  
Permphan Dharmasaroja
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Mrna Level ◽  
Elongation Factor ◽  
Relative Increase ◽  
Mtor Pathway ◽  
Gene Profiling ◽  
Cellular Model ◽  
Protein Elongation

The PI3K/Akt/mTOR pathway is one of dysregulated pathways in Parkinson’s disease (PD). Previous studies in nonneuronal cells showed that Akt regulation can be increased by eukaryotic protein elongation factor 1 alpha 2 (eEF1A2). eEF1A2 is proposed to contribute protection against apoptotic death, likely through activation of the PI3K/Akt pathway. Whether eEF1A2 plays a role in the prevention of cell death in PD has not been investigated. Recently, gene profiling on dopaminergic neurons from postmortem PD patients showed both upregulation and downregulation of some PI3K and mTOR genes. In this paper, the expression of all gene members of the PI3K/Akt/mTOR pathway in relation to those of the eEF1A isoforms in a cellular model of PD was investigated at the mRNA level. The results showed a similar trend of upregulation of genes of the eEF1A isoforms (eEF1A1andeEF1A2) and of the PI3K (classes I–III)/Akt (Akt1,Akt2, andAkt3)/mTOR (mTORC1andmTORC2) pathway in both nondifferentiated and differentiated SH-SY5Y dopaminergic cells treated with 1-methyl-4-phenylpyridinium (MPP+). Upregulation ofeEF1A2,Akt1, andmTORC1was consistent with the relative increase of eEF1A2, Akt, phospho-Akt, and mTORC1 proteins. The possible role of eEF1A isoforms in the regulation of the PI3K/Akt/mTOR pathway in PD is discussed.

scholarly journals Chemical interplay and complementary adaptative strategies toggle bacterial antagonism and co-existence

2021 ◽  
Author(s):  
Carlos Molina-Santiago ◽  
David Vela-Corcía ◽  
Daniel Petras ◽  
Luis Díaz-Martínez ◽  
Alicia Isabel Pérez-Lorente ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Bacterial Species ◽  
Elongation Factor ◽  
Protein Translation ◽  
Cellular Responses ◽  
Cell Membrane Permeability ◽  
Pseudomonas Chlororaphis ◽  
Bacterial Cells ◽  
Bacterial Antagonism ◽  
Protein Elongation

AbstractBacterial communities are in a continuous adaptive and evolutionary race for survival. A myriad of molecules that kill, defend, or mediate communication between bacterial cells of different lineages shape the final structure of the microbial community. In this work we expand our knowledge on the chemical interplay and specific mutations that modulate the transition from antagonism to co-existence between two plant-beneficial bacteria, Pseudomonas chlororaphis PCL1606 and Bacillus amyloliquefaciens FZB42. We reveal that the bacteriostatic activity of bacillaene produced by Bacillus relies on an interaction with the protein elongation factor FusA and how mutations in this protein lead to tolerance to bacillaene and other protein translation inhibitors. Additionally, we describe how the unspecific tolerance to antimicrobials associated with mutations in the glycerol kinase GlpK is provoked mainly by a decrease of Bacillus cell membrane permeability among other pleiotropic cellular responses. We conclude that nutrient specialization and mutations in basic biological functions are bacterial evolutive and adaptive strategies that lead to the coexistence of two primary competitive bacterial species rather than their mutual eradication.

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