scholarly journals Ultrasonic Assisted Seed Priming to Alleviate Aging Damages to Milk Thistle (Silybum marianum) Seeds

2018 ◽  
Vol 10 (2) ◽  
pp. 275-281
Author(s):  
Seyed A. MOOSAVI ◽  
Seyed A. SIADAT ◽  
Adel POSHTDAR ◽  
Fatemeh DIREKVAND
Keyword(s):  
Unsaturated Fatty Acids ◽  
Accelerated Aging ◽  
Aging Treatment ◽  
Seed Priming ◽  
Milk Thistle ◽  
Silybum Marianum ◽  
Ultrasonic Assisted ◽  
Aging Conditions ◽  
Pharmaceutical Properties ◽  
Aged Seeds

Milk thistle is a medicinal plant with high pharmaceutical properties to help relief of liver diseases. In this study, the effects of ultrasonic assisted seeds priming (20, 40, 80 and 160 s) with a frequency of 24 kHz and Power of 400 W was investigated on seed enhancement of aged seeds of Milk thistle. Results of the study, showed that as the aging damages increased, the longer sonication results in the better germination. Root growth was significantly improved using ultrasonic energy. Seeds aged for 24 hours, were exposed to ultrasonic produced for 20 seconds exhibited root length of 10.39 cm which was 5.48 cm with no ultrasonic treatment with the same aging duration. The maximum malondialdehyde activity was observed at the 96-hour aging treatment (87.83 nmol/grFW) while the lowest activity was observed at no aged and 24-hour aged seeds (7.28 nmol/grFW). It is suggested that there is a negative correlation between seedling vigor and MAD activity. It was revealed that fatty acid composition of Silybum marianum seed oil is highly influenced by the aging treatment. The variations of unsaturated fatty acids significantly increased in aged seeds. Our results showed that under accelerated aging conditions, ultrasonic assisted seed priming could not provide satisfactory enhancement to seed germination while no aged seeds germination was significantly improved using ultrasonic assisted seed priming.

scholarly journals Hormone Priming Improves Germination Characteristics and Enzyme Activity of Sorghum Seeds (Sorghum Bicolor L.) Under Accelerated Aging

2013 ◽  
Vol 46 (3) ◽  
pp. 49-56 ◽  
Author(s):  
M.S. Azadi ◽  
S.A. Tabatabaei ◽  
E. Younesi ◽  
M.R. Rostami ◽  
M. Mombeni
Keyword(s):  
Enzyme Activity ◽  
Accelerated Aging ◽  
Aging Treatment ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Germination Index ◽  
Percentage Germination ◽  
Germination Characteristics ◽  
Aged Seeds

Abstract Maximum germination percentage achieves immediately after harvesting and gradually decreases with storage time. Aging is one of the key factors in plant yield loss especially in vegetables. Seed aging is the main problem of seed storage. Application of accelerated aging treatment is used to assess seed vigor and quality. Seed priming enhances seed germination performance after aging. An experiment was conduct in order to investigate the activity of catalase and ascorbate peroxidase during accelerated aging and repair during priming treatment of sorghum seeds. In order to improve germination characteristics in aged seeds with seed priming. Our result showed that seed priming treatments significantly (p≤ 0.01) affected, germination percentage, germination index and means time to germination after aging (0, 3 and 6 days). Increasing aging duration resulted higher reduction in germination characteristics. Priming with gibberelic acid (GA), salicylic acid (SA) and ascorbic acid (ASC) increased germination characteristics of seed aged. The highest germination percentage, germination index, normal seedling percentage and enzyme activity were achieved in control conditions (0 day of aging). Antioxidant activity of aged seeds increased after seed priming.

scholarly journals Introduction Pages

2018 ◽  
Vol 10 (2) ◽  
pp. I-VI
Author(s):  
Radu E. SESTRAS
Keyword(s):  
Life Science ◽  
Seed Priming ◽  
Floristic Diversity ◽  
Forest Types ◽  
Vegetation Analysis ◽  
Silybum Marianum ◽  
Germplasm Collections ◽  
Threat Status ◽  
Forest Division ◽  
Ultrasonic Assisted

Notulae Scientia Biologicae (http://www.notulaebiologicae.ro), Issue 2, Volume 10, 2018: The papers published in this issue (http://www.notulaebiologicae.ro/index.php/nsb/issue/current) represent interesting novelties in different topics of life science. Among the exciting researches, we invite you to find news about: combining microsatellite markers and ampelography for better management of Romanian grapevine germplasm collections; investigation of the breeding mechanism of African yam bean (Sphenostylis stenocarpa); ultrasonic assisted seed priming to alleviate aging damages to milk thistle (Silybum marianum) seeds; floristic diversity, vegetation analysis and threat status of plants in various forest types in Dharmapuri Forest Division, Tamilnadu, Southern India etc. Notulae Scientia Biologicae journal has moved to online-only publication at the start of 2017. At the same time, we maintain our standard publication, as printed form, with 'classic' style - volume, issue, pagination.

scholarly journals The Effect of Seed Priming and Accelerated Aging on Germination and Physiochemical Changes in Milk Thistle (Silybum marianum)

2013 ◽  
Vol 5 (2) ◽  
pp. 204-211 ◽  
Author(s):  
Ghasem PARMOON ◽  
Ali EBADI ◽  
Sodabeh JAHANBAKHSH ◽  
Mahdi DAVARI
Keyword(s):  
Polyphenol Oxidase ◽  
Seedling Growth ◽  
Germination Rate ◽  
Accelerated Aging ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Milk Thistle ◽  
Antioxidants Activity ◽  
Germination Speed

Effects of seed priming and aging on some physiological characteristics of Milk thistle was studied in a factoral experiment based on Complete Randomized Design (CRD). Tratments were included hydro priming (using distilled water), halo priming (0, 1.5, 3, 4.5 and 6% KNO3) and accelerated aging (0, 2, 4 and 6 days under 45°C and 95% humidity) in three replications. Determined parameters were germination charactristics including germination percentage, daily germination speed, mean time of germination, seed vigor index, hypocutile length and hypocutile dry weight. Activity of catalase, peroxidase and polyphenol oxidase were determined at 12 hours after imbibition and seedling stage. According to results of this experiment, germination percentage, seed vigor and seedling growth of seeds were increased under all priming treatments. Improving the catalase and peroxidase activity led to decrease the aging damages. Germination characteristics were improved under both priming treatments at the beginning of germination as well as seedling growth. Polyphenol oxidase activity was increased in the pre-treated seeds but decreased in seedling growth stage. Aging treatments led to reduce the germination percentage, daily germination speed, seed vigor and seedling growth while the germination time was increased. Accelerated aging caused to reduce the germination rate and seedling growth of milk thistle that is probably due to increasing the lipid peroxidation, free radical increment and decreasing the antioxidants activity. The greatest and lowest antioxidants activity, the germination percentage, germination speed and seed vigor were respectively observed under priming using 3% KNO3 concentration and control seeds.

Heat-shock proteins in monitoring aging and heat-induced tolerance in germinating wheat and barley embryos

1998 ◽  
Vol 8 (2) ◽  
pp. 91-98 ◽  
Author(s):  
A. Dell'Aquila ◽  
M. G. Corona ◽  
M. Di Turi
Keyword(s):  
Heat Shock ◽  
Accelerated Aging ◽  
Shock Treatment ◽  
Heat Shock Treatment ◽  
General Decline ◽  
Aging Conditions ◽  
Germination Behaviour ◽  
Induced Tolerance ◽  
Shock Proteins ◽  
Aged Seeds

AbstractWheat and barley seeds were subjected to accelerated aging conditions at 12% moisture content and 35°C temperature over 28 days of storage, and to heat-shock treatment carried out by 4 h of incubation at 40°C following 16 h of imbibition at 20°C. Heat-treated, aged seeds showed altered germination behaviour and increasing leakage electroconductivity as well as a reduced incorporation of [35S]-methionine into embryo proteins, in comparison with the corresponding untreated seeds. Two-dimensional electrophoresis of labelled proteins from embryos gave evidence of further quantitative and qualitative changes: (a) ‘normal’ germination protein synthesis in wheat and barley was slightly modified by age, but following heat-shock treatment a general reduction of most of the control polypeptides occurred; (b) heat-shock response resulted in the production of several HSPs with different MW and pl, but a uniform general decline in their synthesis was not observed. Highly labelled HSPs (e.g., those with MW 66.7 or 89.3–66.7 kDa in wheat or barley, respectively) did not change over the entire period of aging, while some smaller HSPs with MW >29 kDa either decreased in intensity or disappeared. Conversely, specific low molecular weight HSPs (MW 17–14.2 kDa) were synthesized more in extremely aged embryos of both species. The hypothesis that these polypeptides may be used as biochemical markers in monitoring both vigour loss and heat-shock induced tolerance in aged seeds is discussed.

The pathogen of Milk Thistle (Silybum marianum (L.) Gaertn.) in the Czech Republic

2015 ◽  
Vol 5 (1) ◽  
pp. 538-544
Author(s):  
Ivana Safrankova ◽  
Kolackova P ◽  
Rutivckova G
Keyword(s):  
Czech Republic ◽  
Environmental Factors ◽  
Active Compound ◽  
Fungal Species ◽  
Medicinal Herb ◽  
Milk Thistle ◽  
Silybum Marianum ◽  
The Czech Republic

Milk thistle is grown in the Czech Republic as a medicinal herb; silymarin is isolated from its achenes and used for the production of liver and gallbladder medicine. The quality and content of the active compound is influenced not only by environmental factors, but also by pests and pathogens. The occurrence of pests of milk thistle variety Silyb was observed in two localities during the years 2011 2013. In the year 2011 the mycoflora of seeds of four milk thistle varieties was determined. Representatives of 15 species were isolated from the seeds, most of them saprophytic. 21 fungal species were isolated and identified from milk thistle plants during the vegetation; Septoria silybi among the most important ones. Possibilities of protection of milt thistle against pathogens are discussed.

scholarly journals Molecular analysis of genetic diversity in population of Silybum marianum (L.) Gaertn in Egypt

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Marwa Hamouda
Keyword(s):  
Genetic Diversity ◽  
Electrophoretic Pattern ◽  
Issr Markers ◽  
Silybum Marianum ◽  
Inter Simple Sequence Repeats ◽  
Sds Page ◽  
Dna Pattern ◽  
Pharmaceutical Properties ◽  
High Level ◽  
Rapd And Issr

Abstract Background Silybum marianum L. Gaertn is a medicinal plant of unique pharmaceutical properties in the treatment of liver disorders and diabetic nephropathy. Biochemical (SDS-PAGE) and molecular markers such as randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) technologies were used in this work to detect genetic diversity of 14 collections of Silybum marianum population in Egypt. Results The electrophoretic pattern of seed protein gave different molecular weight bands, ranging from 24 to 111 KDa with the presence of unique bands. RAPD results revealed a high level of polymorphism (73.2%) using 12 RAPD primers, but only eight of them gave reproducible polymorphic DNA pattern. Sixteen primers were used in the ISSR method; only ten of them yielded clearly identifiable bands. The percentage of polymorphism is about 80% of the studied samples. Conclusion The obtained data confirmed that SDS-protein, RAPD, and ISSR markers are important tools for genetic analysis for Silybum marianum and recommended to give accurate results.

Field temperature and moisture loads from a building envelope as the basis for accelerated aging of barrier membranes

2019 ◽  
Vol 46 (11) ◽  
pp. 969-978 ◽  
Author(s):  
Marzieh Riahinezhad ◽  
Augusta Eve ◽  
Marianne Armstrong ◽  
Peter Collins ◽  
J.-F. Masson
Keyword(s):  
Building Materials ◽  
National Research Council ◽  
Accelerated Aging ◽  
Bimodal Distribution ◽  
Building Envelope ◽  
Rate Of Change ◽  
Single Family ◽  
Field Exposure ◽  
Aging Conditions ◽  
Materials Used

Temperature and relative humidity (RH) data within the building envelope of a single-family home at the National Research Council of Canada’s Canadian Centre for Housing Technology were collected over five years. We report on the distribution, rate of change, and the limits of temperature and moisture variations for south-easting wall and south-facing wall and roof systems to better understand the in-situ environmental conditions to which building materials and components typical of homes in North America may be subjected. Over an average year, wall temperature varied from −25 °C to +45 °C, and temperature followed a bimodal distribution, with maxima at 0 °C to 5 °C and 15 °C to 20 °C. Each maximum represented about 1100 h of field exposure. Roof temperatures, which spanned a temperature range from −35 °C to 75 °C, did not show a Gaussian distribution but were characterized as being multi-modal. From values of temperature and RH, absolute moisture contents within the building envelope were found to range between 1 and 55 g/m3, with the most common values being 6–8 g/m3. The application of this information is discussed and related to the development of realistic accelerated aging conditions to obtain a more accurate durability assessment of building envelope materials used in Canadian dwellings.

scholarly journals Protective Effects of Milk Thistle (Silybum marianum) against Aflatoxin B1 in Broiler Chicks

2011 ◽  
Vol 24 (7) ◽  
pp. 1011-1018 ◽  
Author(s):  
N. Chand ◽  
Din Muhammad ◽  
F. R. Durrani ◽  
M. Subhan Qureshi ◽  
Sahibzada S Ullah
Keyword(s):  
Aflatoxin B1 ◽  
Milk Thistle ◽  
Broiler Chicks ◽  
Protective Effects ◽  
Silybum Marianum
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