scholarly journals Micropropagation of Inula germanica L. from the Seedlings Explants

2018 ◽  
Vol 46 (1) ◽  
pp. 52-57 ◽  
Author(s):  
Alina TREJGELL ◽  
Monika KAMIŃSKA ◽  
Karolina LISOWSKA ◽  
Andrzej TRETYN
Keyword(s):  
Solid Medium ◽  
Positive Impact ◽  
Shoot Tips ◽  
Fluorescent Light ◽  
First Year ◽  
Naphthaleneacetic Acid ◽  
Multiplication Rate ◽  
Indolebutyric Acid ◽  
Regenerated Plants

This is the first communication of micropropagation system for Inula germanica using seedling explants germinated in vitro. The development of this system gives the possibility of future reintroduction of I. germanica providing a way to stabilize or re-establish its population. Shoot tips and fragments of cotyledons, hypocotyls and roots were isolated from ten-day-old seedlings. Explants were put on MS medium containing 1.0 mg l-1 benzylaminopurine and 0.1 mg l-1 naphthaleneacetic acid and cultured under continuous white fluorescent light (45 μmol.m-2.s-1) at 26 ± 1 °C. The highest percentage of shoot organogenesis (83.3%) was recorded for hypocotyl, while the highest average number of shoots per explant (12.0) was recorded for shoot tips. In subsequent subcultures, multiplication rate decreased to 3.0-4.9 shoots per explant. Less than 19% shoots were able to root on the solid medium without auxins. The highest rooting efficiency (69.3%) was recorded for solid medium supplemented with indolebutyric acid, but growth of roots was inhibited. The percentage of rooted shoots (62.2%) and number of roots per shoot (2.4 per shoot) into the liquid medium were comparable to medium with 0.1 mg·l-1 indolebutyric acid. showing a positive impact on the process of acclimatization. The regenerated plants were able to flowering in the first year after acclimatization. Developed micropropagation system for I. germanica is efficient and can be a useful tool for the active protection of this species.

scholarly journals Micropropagation of White Flowering Eastern Redbud (Cercis canadensis var. alba L.)

1990 ◽  
Vol 8 (4) ◽  
pp. 177-179
Author(s):  
S. Yusnita ◽  
R. L. Geneve ◽  
S. T. Kester
Keyword(s):  
Root System ◽  
Day Treatment ◽  
Shoot Multiplication ◽  
Naphthaleneacetic Acid ◽  
Indolebutyric Acid ◽  
Cercis Canadensis ◽  
Half Strength ◽  
Eastern Redbud ◽  
Being Moved

Abstract A white flowering Eastern redbud (Cercis canadensis var. alba L.) has been successfully micropropagated. Two node explants collected from the initial flush of spring growth were cultured on woody plant medium (WPM). Increased shoot multiplication occurred at 10,15 and 20 μM (2.3, 3.4 and 4.5 ppm) benzyladenine (BA). Microshoots were rooted in vitro on half strength WPM with a 15-day treatment of 100 and 300 μM (18.6 and 55.9 ppm) α-naphthaleneacetic acid (NAA) or 100 and 300 μM (20.3 and 60.9 ppm) indolebutyric acid (IBA) prior to being moved to full strength WPM without growth regulators. Percentage rooting and the mean number of roots per cutting were comparable between NAA and IBA treated microcuttings, however, the subsequent root morphology differed between the two treatments. NAA treated plants developed a coarse, unbranched root system, while IBA treated cuttings developed a more desireable fine, branched root system. Rooted microshoots were successfully acclimated to greenhouse conditions.

scholarly journals Micropropagation and in vitro conservation of Alcantarea nahoumii (Bromeliaceae), an endemic and endangered species of the Brazilian Atlantic Forest

2020 ◽  
Vol 42 ◽  
pp. e52940
Author(s):  
Simone Sacramento dos Santos Silva ◽  
Everton Hilo de Souza ◽  
Fernanda Vidigal Duarte Souza ◽  
Cristina Ferreira Nepomuceno ◽  
Maria Angélica Pereira de Carvalho Costa
Keyword(s):  
Atlantic Forest ◽  
Culture Media ◽  
In Vitro Conservation ◽  
Naphthaleneacetic Acid ◽  
High Survival ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Mature Seeds ◽  
Stem Segments

Alcantarea nahoumii (Leme) J. R. Grant is a species native to the Atlantic Forest that stands out for ornamental purposes. The objective of this work was to evaluate the in vitro germination of A. nahoumii seeds and establish a micropropagation protocol for production of seedlings so as to minimize the effects of predatory extractivism and develop an in vitro conservation system. Mature seeds were disinfested, established in three culture media (MS, MS½ and MS⅓) and incubated at four temperatures (20, 25, 30 and 35ºC) in a germination chamber. In the micropropagation experiment, stem segments were introduced in MS medium supplemented with 0.5 μM of 1-naphthaleneacetic acid (NAA) and 0.0, 2.2, 4.4 and 6.6 μM of 6-benzylaminopurine (BAP). For the in vitro conservation, plantlets were established in MS or MS½ medium supplemented with 15 g L-1 or 30 g L-1 of sucrose. The plants were acclimated with commercial substrate. The highest seed germination percentages were promoted by temperature conditions of 20 and 25ºC, with MS culture medium. The highest multiplication rate of shoots was obtained from the treatment without addition of the growth regulator or when combined with 2.2 μM of BAP + 0.5 μM of NAA. The acclimation of the plants occurred with high survival rate. The species can be conserved in vitro under slow growth condition for 24 months when incubated in MS medium supplemented with 30 g L-1 of sucrose.

scholarly journals Evaluation of the effects of agricultural LED lighting on in vitro propagation of Cordyceps militaris (Link.) Fries

2020 ◽  
Vol 17 (3) ◽  
pp. 473-481
Author(s):  
Do Hong Gam ◽  
Duong Huong Huynh ◽  
Phan Thi Lan Anh ◽  
Nguyen Hoang Duong ◽  
Do Thi Kim Hoa
Keyword(s):  
Growth And Development ◽  
In Vitro Propagation ◽  
Positive Impact ◽  
The Other ◽  
Fluorescent Light ◽  
Led Lighting ◽  
Fresh Biomass ◽  
Red Led ◽  
Led Lights

In this study, the effects of various agricultural LED lights (LED NN), including single red LED (R), single blue LED (B), and four combinations of blue, red, and warm white (W) LED (BR, BRW1, BRW2, BRW3) on the growth and development of C. militaris (Link.) Fries were evaluated in vitro. After 7 days, samples subjected to LED NN showed shorter sporocarp sprouting time and higher sprouting ratio than the control, which was subjected to T5 fluorescent light. After 2 months, LED lights with high red ratio, such as single red LED and LED BR, had suppressing effect on the growth and development of C. militaris (Link.) Fries. On the other hand, combinations of red, blue, and warm white such as LED BRW1, LED BRW2, and LED BRW3 had the positive impact on the growth and development of this fungus. Notably, samples subjected to LED BRW2 reached 5.79 cm in height, fresh biomass of 3.67 g/20 samples. Cordycepin and Adenosine levels were 64.2 and 6.37 mg/100 g fresh mass, respectively. All of studied  indicators were the higher compared to those of the control and other LED lighting schemes. Therefore, it can be conlcuded that LED lighting combination with BRW2 ratio of 1:5:1 and luminous intensity of 45±2 µmol.m-2.s-1 (511,59 Lux) was suitable for the growth and development of C. militaris (Link.) Friesand a potential replacement of fluorescent light for C. militaris (Link.) Friesin vitro propagation.

scholarly journals In vitro Multiplication of Iraca palm (Carludovica palmata Ruíz & Pavón)

2020 ◽  
Vol 73 (1) ◽  
pp. 9039-9046
Author(s):  
Rodrigo Alberto Hoyos Sanchez ◽  
Diego Chicaíza Finley ◽  
Juan Carlos Zambrano Arteaga
Keyword(s):  
Root Formation ◽  
The Other ◽  
Naphthaleneacetic Acid ◽  
Multiplication Rate ◽  
In Vitro Multiplication ◽  
Commercial Interest ◽  
Other Hand ◽  
Number Of Shoots

Carludovica palmata Ruíz & Pavón is a plant that belongs to the Cyclanthaceae family. Its commercial interest is related to the production of fibers for the manufacture of handicrafts, mainly the Panama hat, so it is important to study its propagation. This investigation aimed to determine the effect of 6-benzylaminopurine (BAP) in the formation of new shoots and 1-naphthaleneacetic acid (NAA) in the formation of roots, as well as the adaptation in greenhouse conditions of Carludovica palmata Ruíz & Pavón. In order to find the optimal multiplication rate, 0.5 cm length explants were planted in glass jars with 15 mL of semisolid MS with different concentrations of BAP and cultured under in vitro conditions for 90 days. The multiplication parameters in this stage were number of shoots per explant (NSE), length of shoots (LS), and length of roots (LR) as multiplication parameters. In a similar procedure, the number of roots per explant (NRE), length of roots (LR), and length of plantlets (LP) was determined using different concentrations of NAA. Finally, different substrates were evaluated for the adaptation of plantlets of C. palmata produced in vitro, under greenhouse conditions for 80 days. The highest multiplication rate (17±3 shoots per explant) was obtained with 2.0 mg L-1 of BAP. Root formation occurred efficiently in all treatments, without significant statistical differences between them. On the other hand, the use of substrate soil-t15 was the best treatment for the growth of C. palmata under greenhouse conditions. From the results obtained, it is concluded that C. palmata can be efficiently multiplied under in vitro conditions and did not present problems during the in vivo rooting process.

scholarly journals Plant regeneration from in vitro leaves of four commercial Pyrus species

2008 ◽  
Vol 54 (No. 4) ◽  
pp. 140-148 ◽  
Author(s):  
H. Tang ◽  
Y. Luo ◽  
C. Liu
Keyword(s):  
Plant Regeneration ◽  
Shoot Regeneration ◽  
Pyrus Communis ◽  
Naphthaleneacetic Acid ◽  
Complete Medium ◽  
Regeneration Frequency ◽  
Regenerated Plants ◽  
Equivalent Conditions

An efficient shoot regeneration from in vitro leaf sections of <I>Pyrus communis</I> Bartlett, <I>P. pyrifolia</I> Shenbuzhi, <I>P. bretschneideri</I> Zaosu and <I>P. ussuriensis</I> Manyuanxiang was successfully developed for use in future transgenic studies. On the basis of regeneration frequency and average shoot numbers, optimal shoot regeneration was obtained on leaf sections of <I>P. communis</I> Bartlett when cultured on Murashige and Skoog complete medium containing 6.0 mg/l BA (6-benzyladenine) and 0.1 mg/l NAA (&alpha;-naphthaleneacetic acid), while Quoirin and Lepoivre complete medium supplemented with 1.0 mg/l TDZ [thidiazuron (N-phenyl-N<sup>1</sup>-1,2,3-thiadiazol-5-ylurea)] and 0.1 mg/l NAA was found best for <I>P. pyrifolia</I> Shenbuzhi, and Nitsch and Nitsch complete medium containing 3.0 mg/l TDZ and 0.1 mg/l NAA or 0.2 mg/l IAA was suitable for<I>P. bretschneideri</I> Zaosu or <I>P. ussuriensis</I> Manyuanxiang, respectively. After cutting the leaves into three sections perpendicular to the midrib and culturing under the equivalent conditions, regeneration occurred more frequently on basal sections than middle sections, and no shoots formed on apical sections. A ratio of NH<sup>+</sup><sub>4</sub>-N/NO<sup>-</sup><sub>3</sub>- N of 1:2~1:7 was found beneficial for shoot regeneration. 75.0–87.5% of proliferating shoots formed roots after 4 weeks of transfer to 1/4 strength of Murashige and Skoog complete medium supplemented with 2.5 mg/l IBA (indole-3-butryric acid) and 30.0 g/l sucrose. Regenerated plants were successfully established under greenhouse conditions.

scholarly journals In vitro propagation of sacha inchi through organogenesis

2018 ◽  
Vol 53 (11) ◽  
pp. 1285-1288 ◽  
Author(s):  
Reynaldo Solis ◽  
Danter Cachique ◽  
Juan Carlos Guerrero-Abad ◽  
María Emilia Ruiz Sánchez ◽  
Lourdes Tapia y Figueroa
Keyword(s):  
In Vitro Propagation ◽  
Naphthaleneacetic Acid ◽  
Indolebutyric Acid ◽  
In Vitro Organogenesis ◽  
Apical Meristems ◽  
Hormonal Balance ◽  
Plukenetia Volubilis ◽  
Sacha Inchi

Abstract: The objective of this work was to evaluate hormonal balance in the phases of the in vitro organogenesis of apical meristems of sacha inchi (Plukenetia volubilis). For the establishment and multiplication phases, Murashige & Skoog (MS) media, with different concentrations of benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), were used. For rooting, modified MS media was supplemented with different concentrations of NAA and indolebutyric acid. The differentiation of apical meristems was possible with 0.1 mg L-1 BAP and 0.05 mg L-1 NAA. The best rooting of seedlings is obtained with 0.5 mg L-1 naphthaleneacetic acid and 2.0 mg L-1 indolebutyric acid.

scholarly journals In vitro Mass Clonal Propagation of Spathoglottis plicata Blume

1970 ◽  
Vol 19 (2) ◽  
pp. 151-160 ◽  
Author(s):  
Pinaki Sinha ◽  
M. Lokman Hakim ◽  
M. Firoz Alam
Keyword(s):  
Clonal Propagation ◽  
Nodal Segments ◽  
Fluorescent Light ◽  
Plantlet Formation ◽  
The Third ◽  
Regenerated Plants ◽  
Rooting Medium ◽  
Half Strength ◽  
In Vitro Clonal Propagation

For in vitro clonal propagation of Spathoglottis plicata Blume nodal segments of young shoots were cultured on half strength of MS  with  2% sucrose + 2.0 mg/l BA + 0.5 mg/l NAA + 2 g/l peptone + 15% (v/v) CW + 0.5 g/l AC,  incubated at 24 ± 2ºC under 3000 lux fluorescent light for a 16 hr photoperiod per day. About 19 micro-shoots were induced from the explants within 12 weeks. Subculture of micro-shoots for eight weeks on the same nutrient medium enhanced the number of micro-shoots up to 60. The clumps of the micro-shoots were dissected and cultured on half strength of MS  with 2% sucrose + 2 g/l peptone + 15% (v/v) CW + 0.5 g/l AC + 200 mg/l L-glutamine. The micro-shoot sections elongated to form shoots, and new micro shoots were induced from the base within eight weeks of culture. For plantlet formation the best rooting medium was determined as  half strength of MS  with 2% sucrose + 2 g/l peptone + 15% (v/v) CW + 0.5 g/l AC + 50 g/l banana pulp. After rearing 25 g mixture of urea, TSP and MOP (2 : 1 : 1) were applied per plant at three months intervals. All the regenerated plants blossomed on the third year. Key words: Spathoglottis plicata, Clonal propagation, Acclimation D.O.I. 10.3329/ptcb.v19i2.5432 Plant Tissue Cult. & Biotech. 19(2): 151-160, 2009 (December)

scholarly journals Solasodine accumulation in regenerated plants of Solanum torvum Sw

2010 ◽  
Vol 12 (1) ◽  
pp. 73-79 ◽  
Author(s):  
C.B Moreira ◽  
S.S Lima ◽  
M.A Esquibel ◽  
A Sato
Keyword(s):  
Indoleacetic Acid ◽  
Basal Medium ◽  
Spectrophotometric Assay ◽  
Naphthaleneacetic Acid ◽  
Nodal Segment ◽  
Benzyl Adenine ◽  
Solanum Torvum ◽  
Regenerated Plants ◽  
Great Production

A nodal segment culture was developed in order to assess Solanum torvum Sw. regeneration and solasodine levels. The influence of auxins (indoleacetic acid, 1-Naphthaleneacetic acid) and benzyl adenine on S. torvum growth in micropropagation was investigated. A nodal segment culture was initiated with seeds germinated in MS basal medium added of GA3 and grown in different concentrations of IAA, IAA + BAP and NAA + BAP. Sixty-day-old plants from the in vitro culture were collected, frozen and lyophilized; then, the methyl orange method was used to quantify solasodine for the spectrophotometric assay. The best results regarding plant regeneration and solasodine accumulation were obtained by using the MS basal medium without addition of plant growth regulators; however, there was great production of calluses presenting friable bases. Based on these results, cell cultures can be initiated from such calluses with application of other auxins and cytokinins to enhance solasodine production, besides different elicitors, light intensities and sucrose concentrations.

scholarly journals Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants

2010 ◽  
Vol 32 (2) ◽  
pp. 612-617 ◽  
Author(s):  
Fernanda Vidigal Duarte Souza ◽  
Ana Maria Mascarenhas Eloy Canto ◽  
Antônio da Silva Souza ◽  
Maria Angélica Pereira de Carvalho Costa
Keyword(s):  
Gibberellic Acid ◽  
Culture Medium ◽  
Residual Effect ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Proliferation Medium ◽  
Regenerated Plants ◽  
Subsequent Regeneration ◽  
Previous Step

This work aimed to evaluate the influence of naphthaleneacetic acid (NAA) and gibberellic acid (GA3) plant regulators in in vitro etiolation and subsequent regeneration of the PE x SC-60 pineapple hybrid. Nodal segments of in vitro plants with approximately 5-7 cm height were incubated in basic MS culture medium supplemented with 0.0; 0.5 and 1.0 mg L-1 of naphthaleneacetic acid (NAA) in combination with gibberellic acid (GA3) in concentrations of 0.0; 0.5 and 1.0 mg L-1, and maintained at 27 ºC under dark condition. Evaluations were carried out at 90 and 180 days after incubation period. The best results for length of etiolated stems were obtained with 1.0 mg L-1 of NAA. In the experiment followed by the regeneration, stems with 3 cm from the etiolation treatment, were cultivated in proliferation medium and the number of regenerated plants per treatment was evaluated at 60 days of cultivation. The treatment that promoted the best etiolation of plants also promoted the worst regeneration rates, demonstrating the residual effect of the auxin used in the previous step in the regeneration of plants of the pineapple hybrid evaluated.

scholarly journals Ex situ Conservation of Three Endemic and/or Endangered Dianthus Species

2013 ◽  
Vol 41 (1) ◽  
pp. 73 ◽  
Author(s):  
Victoria CRISTEA ◽  
Liliana JARDA ◽  
Irina HOLOBIUC
Keyword(s):  
Ex Situ Conservation ◽  
Positive Impact ◽  
Botanical Garden ◽  
Ex Situ ◽  
First Year ◽  
Genetic Uniformity ◽  
Current Context ◽  
Different Populations

Within the current context of declining biodiversity, the botanical gardens play an essential role in its conservation. Dianthus callizonus, D. glacialis ssp. gelidus and D. spiculifolius are the species that we seek to preserve in "Alexandru Borza" Botanical Garden of Cluj-Napoca (Romania). Several replicates were collected for each taxon from different populations in order to avoid the genetic uniformity. The material collected from the natural sites, was planted on a rockery, specially designed for this collection in the Botanical Garden. At the time of planting, each individual was sampled for setting up an in vitro collection and further biochemical and molecular analyses. In case of ex situ outdoor conservation of the three Dianthus species, 80.6% of the individuals collected in the field survived during the first year but the percentage decreased drastically after four years. In the case of in situ collected individuals, as well as in the case of in vitro individuals, D. spiculifolius had the best ability to acclimatize in the Botanical Garden, and D. callizonus presented the lowest number of surviving individuals. The ex vitro acclimatization of the plantlets had 80% efficiency at 10ºC, using three different substrates: soil and pearl stone mix 1/1, soil and sand mix 1/1 and pearl stone. All the three species are preserved in vitro, whereas the plantlets are acclimatized outdoors. Ex situ conservation of these species will have a positive impact on the biodiversity conservation.

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