scholarly journals Polyphenolic Profile, Anti-Inflammatory and Antinociceptive Activity of an Extract from Arctium lappa L. Roots

2017 ◽  
Vol 45 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Simona CONEA ◽  
Cristina MOGOSAN ◽  
Oliviu VOSTINARU ◽  
Claudia Crina TOMA ◽  
Ioana CUC HEPCAL ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Writhing Test ◽  
Arctium Lappa ◽  
Paw Oedema ◽  
Antinociceptive Effects ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Polyphenolic Profile ◽  
Dose Dependent ◽  
Rat Paw Oedema

The aim of the study was to assess the polyphenolic profile, anti-inflammatory and antinociceptive activity of Arctium lappa, a medicinal plant traditionally used in the treatment of gout, hepatitis and other inflammatory disorders. Polyphenolic profile of a hydro-glycero-ethanolic extract from Arctium lappa roots (ALE) was evaluated by HPLC-MS method. Anti-inflammatory effect of the Arctium lappa extract (ALE) was determined by carrageenan-induced rat paw oedema test, while antinociceptive effect was determined by acetic acid induced writhing test in mice and Randall Selitto test in rats. HPLC-MS analysis of the extract showed the presence of chlorogenic acid (158.9 μg/mL) and quercitrin (14.4 μg/mL). The administration of ALE reduced the oedema formation in the carrageenan-induced rat paw oedema test, especially at dose of 500 mg/kg, the results being statistically significant and dose-dependent. Also, ALE showed statistically significant and dose-dependent antinociceptive effects in the acetic acid induced writhing test in mice and Randall Selitto test in rats. The results of the pharmacological experiments suggest that the anti-inflammatory and antinociceptive effects of the Arctium lappa extract (ALE) may be related to the ability of polyphenols such as chlorogenic acid to inhibit the synthesis and release of some pro-inflammatory mediators. Our experimental data justify the traditional use of this plant in the management of some inflammatory disease.

scholarly journals Anti-inflammatory and Anti-arthritic Activities ofMerremia tridentate(L.) Hall. f.

2009 ◽  
Vol 6 (4) ◽  
pp. 943-948 ◽  
Author(s):  
M. Kamalutheen ◽  
S. Gopalakrishnan ◽  
T. Syed Ismail
Keyword(s):  
Ethanol Extract ◽  
Albino Rats ◽  
Standard Drug ◽  
Arthritis Model ◽  
Paw Oedema ◽  
Dependent Activity ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Dose Dependent ◽  
Rat Paw Oedema

The various extracts ofMerremia tridentatewere investigated for its anti-inflammatory and anti-arthritic activities in male albino rats. The anti-inflammatory activity was carried out using carrageenan-induced rat paw oedema model and the anti-arthritic activity was carried out using complete Freund’s adjuvant induced arthritis model. Indomethacin (10 mg/kg bw) was used as a standard drug. The ethanol extract ofM. tridentateexhibited significant dose dependent activity in acute inflammation and the doses of 100 mg/kg bw and 200 mg/kg bw produced 38.3% and 42.8% inhibition respectively after 3 h as compared with that of the standard drug which showed 48.5% inhibition. In arthritis model, the doses of 100 mg/kg bw and 200 mg/kg bw of the ethanol extract produced 49.0% and 51.7% inhibition respectively after 19 days when compared with that of the standard drug (55.5%). Both doses of the ethanol extract ofM. tridentateexhibit significant anti-inflammatory and anti-arthritic activities.

scholarly journals Anti-inflammatory activity of Syzygium cumini leaf against experimentally induced acute and chronic inflammations in rodents

2011 ◽  
Vol 1 (1) ◽  
pp. 6 ◽  
Author(s):  
Anirban Roy ◽  
Sanjib Bhattacharya ◽  
Jitendra N. Pandey ◽  
Moulisha Biswas
Keyword(s):  
Inflammatory Activity ◽  
Dependent Manner ◽  
Syzygium Cumini ◽  
Large Size ◽  
Paw Oedema ◽  
Chronic Models ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Dose Dependent ◽  
Rat Paw Oedema

<p><em>Syzygium cumini<strong> </strong></em>(L.) Skeels (Myrtaceae), commonly known as <em>Jam </em>in Bengali, <em>Jamun</em> in Hindi and <em>Black Plum</em> or <em>Black Berry</em> in English, is a large size evergreen tree indigenous to India and is cultivated for its fruits. In the present study, the methanol extract of leaves from <em>S. cumini </em>(MESC) was evaluated for anti-inflammatory activity in experimental acute (carrageenan, histamine and serotonin induced rat paw oedema) and chronic models (cotton pellet induced rat granuloma). In all models, the MESC (100 and 200 mg/kg body wt. p.o.) exhibited significant anti-inflammatory activity (<em>p &lt; 0.001</em>) in a dose dependent manner. These findings revealed that the <em>S. cumini </em>leaf had remarkable acute and chronic anti-inflammatory actions in the tested rodent models.</p>

scholarly journals Anti-allergic and anti-inflammatory activities of black cumin extracts in in vitro and in vivo model systems

2021 ◽  
Author(s):  
Nazma Shaheen ◽  
Afiatul Azam ◽  
Amlan Ganguly ◽  
Saeed Anwar ◽  
Md Sorwer Alam Parvez ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Paw Edema ◽  
Writhing Test ◽  
Black Cumin ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Dose Dependent

Black cumin (Nigella sativa) is a widely used ingredient of traditional medicine for its broad-spectrum pharmacological actions, including anti-allergic, bronchial asthma, and anti-inflammatory properties. We sought to evaluate BC extracts' efficacy for their anti-allergic and anti-inflammatory properties using a comprehensive in vitro, in vivo, and silico experimental setup. To investigate whether BC extract has anti-inflammatory, anti-allergic, and analgesic therapeutic potentials in vitro and in vivo. The activity of BC was assessed through anti-allergic activity on rat basophilic leukemia-2H3 cell line, anti-inflammatory activity on J774.1A cell line, anti-inflammatory activity by carrageenan-induced rat paw edema, analgesic activity by acetic acid-induced writhing test, and ingenuity analysis of the BC extracts in inflammation control. BC exerted potent anti-allergic activity by inhibiting antigen-induced degranulation. An anti-inflammatory effect is shown by inhibiting TNF-α pro-duction. The acetic acid-induced writhing test shown a dose-dependent reduction of writhing number following BC administration. Rat paw edema test shown the dose-dependent reduction of paw edema volume following BC administration. Ingenuity Pathway Analysis (IPA) suggested BC extracts containing ferulic acid, p-coumaric acid, kaempferol, and quercetin can inhibit inflammation. This study suggests that bioactive compounds in BC extract act as an anti-allergic and anti-inflammatory agent by regulating several downstream and upstream inflammation pathways.

scholarly journals Antinociceptive and Anti-Inflammatory Activities ofBridelia retusaMethanolic Fruit Extract in Experimental Animals

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Tekeshwar Kumar ◽  
Vishal Jain
Keyword(s):  
Acetic Acid ◽  
Animal Models ◽  
Methanolic Extract ◽  
Significant Inhibition ◽  
Dependent Manner ◽  
Paw Oedema ◽  
Antinociceptive Effects ◽  
Anti Inflammatory ◽  
Wbc Count ◽  
Dose Dependent

Antinociceptive and anti-inflammatory potentials of methanolic extract ofBridelia retusafruit (BRME) were evaluated against different animal models in rodents. Antinociceptive effects of BRME were assessed in mice using the acetic acid-induced writhing and formalin test. Anti-inflammatory effects of BRME in three different doses, namely, 100, 200, and 400 mg/kg, were evaluated by utilizing different animal models representing various changes associated with inflammation, namely, carrageenan-induced paw oedema, histamine and serotonin-induced paw oedema, arachidonic acid-induced paw oedema, formalin-induced paw oedema, TPA-induced ear oedema, acetic acid-induced vascular permeability, total WBC count in paw fluid, and myeloperoxidase assay. Also BRME was phytochemically evaluated using chromatographic method. The BRME did not exhibit any signs of toxicity up to a dose of 2000 mg/kg. The extract showed statistical significant inhibition of induced nociception and inflammation in dose dependent manner. The higher dose of extract significantly inhibited pain and inflammation against control (P<0.001). HPLC results revealed the presence of gallic acid and ellagic acid as phytoconstituents in BRME and it was proven as anti-inflammatory agents. The present study scientifically demonstrated the antinociceptive and anti-inflammatory potential of fruit ofB. retusamethanolic extract. These effects may be attributed to the presence of polyphenolic phytoconstituents in the extract.

scholarly journals Analgesic and Anti-inflammatory Activities of Triterpenoid Molecules Isolated from the Leaves of Combretum glutinosum Perr. Ex DC (Combretaceae)

2019 ◽  
pp. 1-8
Author(s):  
Madièye Sene ◽  
Daouda Ndiaye ◽  
Abdoulaye Gassama ◽  
Firmin Sylva Barboza ◽  
Mbaye Diagne Mbaye ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Medicinal Plant ◽  
Acetylsalicylic Acid ◽  
Traditional Medicine ◽  
Control Group ◽  
Paw Oedema ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Rat Paw Oedema

Combretum glutinosum Perr. Ex DC (COMBRETACEAE) is a traditional medicinal plant, widely distributed in Senegal and Africa. The aim of that study was to investigate the analgesic and anti-inflammatory activities of two triterpenes, betulonic acid (DN7) and cabraleone (DN12), isolated from the leaves of Combretum glutinosum. Experiments were performed in acetic acid-induced contortions in mice and carrageenan rat paw oedema. DN7 (3 mg/kg, per os) and DN12 (3 mg/kg, per os) significantly prevent contortions in mice. The number of contortions is respectively 30±10 and 32±7 versus 72.6±6.64 in control group (p<0.05, n=5). DN7 (3 mg/kg, per os) significantly prevented the increased rat paw oedema (31.84±6.76 vs 92.72±6.05%) (p<0.05, n=5). DN12 (10 mg/kg, per os) induced rat paw oedema prevention similar to that of acetylsalicylic acid (10 mg/kg, per os) administered in the same conditions (29.28±5.88 vs 30.96±7.25%; ns, n=5). The analgesic and anti-inflammatory activities of triterpenes isolated from C. glutinosum leaves are similar to those of non-steroidal anti-inflammatory drugs such acetylsalicylic acid, justifying the use of this plant in traditional medicine to manage pain and inflammation.

scholarly journals Anti-inflammatory potentials, membrane stabilizing and xanthine oxidase inhibitory activities of Clerodendrum volibule ethanolic leaf extract on carragenaan- induced inflammation in rats

2018 ◽  
Vol 6 (1) ◽  
pp. 7 ◽  
Author(s):  
Olarenwaju Olufunmilayo. ◽  
OLOGE Mary ◽  
Oyemitan Idris ◽  
AKOMOLAFE Rufus ◽  
Akinpelu Bola ◽  
...  
Keyword(s):  
Xanthine Oxidase ◽  
Leaf Extract ◽  
Dependent Manner ◽  
Inflammatory Conditions ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Dose Dependent ◽  
Ethanolic Leaf Extract ◽  
Rat Paw Oedema

The folkloric use of Clerodendrum volubile P Beauv (Verbenaceae) for treatment of inflammatory conditions in the Southern part of Nigeria has been reported. The anti-inflammatory action of the ethanolic leaf extract on carrageenan-induced rat paw oedema, lipoxygenase and xanthine oxidase inhibitory effects, and membrane stabilizing potential were evaluated. The extract reduced carrageenan-induced rat paw oedema in a dose dependent manner compared to control group. C. volubile inhibited xanthine oxidase activity in a dose - dependent manner; at 0.5 mg/ml it exhibited maximum inhibitory activity of 95.48% while the standard drug, allopurinol exhibited 70 % inhibition. The extract significantly inhibited lipoxygenase activity, with highest activity at 0.4 mg/ml while Quercetin showed maximum inhibition of the enzyme at 0.1 mg/ml. The extract and Acetaminophen exhibited maximum membrane stabilizing activity of 91.85 ± 0.50% and 94.32 ± 0.32% at 2.5 mg/ml respectively. These findings provide justification for the traditional use of C. volubile in inflammatory conditions.

scholarly journals Antinociceptive and Anti-Inflammatory Activities of Leaf Methanol Extract ofCotyledon orbiculataL. (Crassulaceae)

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
George J. Amabeoku ◽  
Joseph Kabatende
Keyword(s):  
Acetic Acid ◽  
Reaction Time ◽  
Plant Species ◽  
Methanol Extract ◽  
Thermal Stimulation ◽  
Hot Plate ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Rat Paw Oedema

Leaf methanol extract ofC. orbiculataL. was investigated for antinociceptive and anti-inflammatory activities using acetic acid writhing and hot-plate tests and carrageenan-induced oedema test in mice and rats, respectively.C. orbiculata(100–400 mg/kg, i.p.) significantly inhibited acetic acid-induced writhing and significantly delayed the reaction time of mice to the hot-plate-induced thermal stimulation. Paracetamol (300 mg/kg, i.p.) significantly inhibited the acetic acid-induced writhing in mice. Morphine (10 mg/kg, i.p.) significantly delayed the reaction time of mice to the thermal stimulation produced with hot plate. Leaf methanol extract ofC. orbiculata(50–400 mg/kg, i.p.) significantly attenuated the carrageenan-induced rat paw oedema. Indomethacin (10 mg/kg, p.o.) also significantly attenuated the carrageenan-induced rat paw oedema. The LD50value obtained for the plant species was greater than 4000 mg/kg (p.o.). The data obtained indicate thatC. orbiculatahas antinociceptive and anti-inflammatory activities, justifying the folklore use of the plant species by traditional medicine practitioners in the treatment of painful and inflammatory conditions. The relatively high LD50obtained shows thatC. orbiculatamay be safe in or nontoxic to mice.

Fibrinolytic, Anti-Inflammatory and Cytotoxic Potentialities of Extracts and Chemical Constituents of Manglicolous Lichen, Graphis ajarekarii Patw. & C. R. Kulk

2020 ◽  
Vol 10 (1) ◽  
pp. 87-93 ◽  
Author(s):  
Vinay Bharadwaj Tatipamula ◽  
Girija Sastry Vedula
Keyword(s):  
Protein Denaturation ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Fibrin Clot ◽  
Hydroalcoholic Extract ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Rat Paw Oedema

Background: Lichens which are betide to mangroves are termed as Manglicolous Lichens (ML). As these ML are habituated under stress conditions, they are screened for unique metabolites and biological activities. Objective: The study aimed to establish the chemical and biological profile of ML, Graphis ajarekarii. Methods: The Ethyl Acetate Extract of G. ajarekarii (EAE) was subjected to chromatographic techniques and the obtained isolates were characterized by spectroscopic analysis. The hydroalcoholic extract of G. ajarekarii (AE), EAE, isolates and Hydroalcoholic Extract of host (HE) were evaluated for fibrinolytic (fibrin clot method), in vitro (protein denaturation method) and in vivo (formalin-induced rat paw oedema assay), anti-inflammatory and cytotoxicity (MTT assay) activities. Results: Chemical investigation of the EAE led to the isolation of two known compounds namely atranorin (1) and ribenone (2), which were confirmed by spectral data. The AE and EAE gradually lysed the fibrin clot with 94.54 and 65.07%, respectively, at 24 h. The AE inhibited protein denaturation of about 88.06%, while the standard (Indomethacin) with 93.62%. Similarly, the in vivo antiinflammatory analysis of AE (200 mg/mL) showed potent reduction of rat paw oedema than the standard, whereas EAE and 1 depicted moderate depletion. In addition, the AE revealed prominence inhibition on MCF-7, DU145 and K-562 with IC50 values of 69.5, 42.5 and 38 µg/mL, respectively, whereas the HE exhibited mild inhibitory profile against fibrin clot, inflammation and cancer. Conclusion: From the results, it can be concluded that the G. ajarekarii has an aptitude to act against coagulation, inflammation and cancer cells.

Anti-Inflammatory Constituents of Alchornea cordifolia Leaves

2015 ◽  
Vol 19 ◽  
pp. 60-64
Author(s):  
AA Ahmadu ◽  
A Agunu ◽  
EM Abdurrahman
Keyword(s):  
Medicinal Plant ◽  
West Africa ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Methyl Gallate ◽  
Paw Oedema ◽  
Analgesic Agents ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Rat Paw Oedema

Alchornea cordifolia (Schum and Thonn) Muell. Arg. (Euphorbiaceae) is a traditional medicinal plant widely distributed in West Africa including Nigeria.The plant has been used for ethnomedicinal purposes against wounds, ulcers, and sores. The decoction of the leaves has been reported to provide remedies for bronchial problems, rheumatic pain and cough. From the dichloromethane and ethyl acetate soluble parts of the Methanol leaf extract, two compounds namely Lup-20(29)-en-3c-ol (lupenol) and Methyl 3, 4,5-trihydroxy benzoate (Methyl gallate) were isolated and their structures elucidated. Anti-inflammatory and analgesic properties of the compounds on carrageenan-induced paw oedema and formalin-induced pain in rats showed that compound 2 significantly (P<0.05) inhibit rat paw oedema compared to the standard drugs (Piroxicam and Morphine) used, while on formalin-induced pain in rats, the same trend was observed and were both comparable to Piroxicam and morphine, the standard anti-inflammatory and analgesic agents used, respectively. Compound 1 did not show any significant anti-inflammatory activity compared to control, likewise compound 2. Thus, compound 2, Methyl trihydroxy benzoate, might be responsible for the anti-inflammatory and analgesic properties of this plant.Keywords: Alchornea cordifolia, Anti-inflammatory, Analgesic, Methyl Trihydroxy Benzoate

scholarly journals A New Cytotoxic Sesquiterpene and Three Anti-inflammatory Flavonoids from Egyptian Tanacetum Santolinoides

2007 ◽  
Vol 2 (11) ◽  
pp. 1934578X0700201 ◽  
Author(s):  
Sabrin R. M. Ibrahim ◽  
Jihan M. Badr ◽  
Khalid A. El Sayed ◽  
Diaa T. A. Youssef
Keyword(s):  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Methanolic Extract ◽  
Spectral Characteristics ◽  
Mass Spectral ◽  
Aerial Parts ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Rat Paw Oedema

A re-investigation of the methanolic extract of the aerial parts of Tanacetum santolinoides has resulted in the isolation of a new sesquiterpene, tanacetonic acid (1), along with three known methoxylated flavonoids. The structure of the isolated compounds was secured based on their IR, UV, 1D and 2D (1H-1H COSY, HSQC and HMBC) NMR spectroscopic and FAB mass spectral characteristics. The new sesquiterpene showed a significant cytotoxic activity when tested using the brine shrimp bioassay, while the three flavonoids demonstrated anti-inflammatory effects in the rat paw oedema test.

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