scholarly journals Simultaneous HPLC Assay of Paracetamol and Sulfapyridine as Markers for Estimating Gastrointestinal Transit of Amphotericin B-containing Nanoparticles in Rat Plasma

2015 ◽  
Author(s):  
Hilda A ◽  
Nashiru B ◽  
Kah-Hay Y
Keyword(s):  
Amphotericin B ◽  
Gastrointestinal Transit ◽  
Rat Plasma ◽  
Hplc Assay

scholarly journals A sensitive HPLC-FLD method for the quantification of 6-O-demethylmenisporphine isolated from Menispermi Rhizoma in rat plasma

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jinxia Wei ◽  
Jia Shao ◽  
Yanan Li ◽  
Yubo Li
Keyword(s):  
Mobile Phase ◽  
Internal Standard ◽  
Rat Plasma ◽  
Hplc Assay ◽  
Isocratic Elution ◽  
Fluorescence Detector ◽  
Limit Of Quantification ◽  
The Mean ◽  
Preclinical Safety ◽  
Concentration Levels

Abstract Background To investigate the pharmacokinetics of 6-O-demethylmenisporphine, an oxoisoaporphine alkaloid with significant anti-tumor activities and isolated from Menispermi Rhizoma, a novel and sensitive HPLC assay was established for 6-O-demethylmenisporphine quantification in rat plasma. Methods Peak responses were detected by a highly selective and sensitive fluorescence detector with 426-nm excitation and 514-nm emission wavelengths. Curcumin was employed as the internal standard (IS). A Capcell Pak C18 column (150 mm × 4.6 mm i.d., 5 μm) and an isocratic elution procedure with a flow rate of 1.0 mL/min were used to exclude the endogenous interfering substance. Acetonitrile-water (68:32, v/v) containing 1% formic acid was employed as mobile phase. A 7-point calibration curve that covered the concentration range of 10–2500 ng/mL was constructed. Results A good linearity was observed with a correlation coefficient (r) of 0.9993. The lower limit of quantification for 6-O-demethylmenisporphine was 10 ng/mL. The mean recoveries of analyte in rat plasma exceeded 80.5%. The precision at four concentration levels was within 11.3% and the accuracy ranged from − 7.6 to 6.7%. Conclusion Using this new HPLC-FLD method, the investigation of plasma samples from rats following oral dosing of neat compound and Menispermi Rhizoma extract was successfully conducted. The results will provide a reference for the evaluation of preclinical safety of 6-O-demethylmenisporphine.

HPLC assay for determination of amphotericin B in biological samples

2008 ◽  
Vol 22 (4) ◽  
pp. 402-407 ◽  
Author(s):  
R. Espada ◽  
J. M. Josa ◽  
S. Valdespina ◽  
M. A. Dea ◽  
M. P. Ballesteros ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Biological Samples ◽  
Hplc Assay

An HPLC assay utilizing solid-phase extraction for CI-1010, an alkylating radiosensitizer, in rat plasma

1997 ◽  
Vol 16 (1) ◽  
pp. 47-55 ◽  
Author(s):  
William W. Bullen ◽  
David T. Rossi ◽  
Keith L. Hoffman ◽  
Ajit Suri ◽  
Chetan D. Lathia
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Rat Plasma ◽  
Phase Extraction ◽  
Hplc Assay

Quantitation of paclitaxel in micro-sample rat plasma by a sensitive reversed-phase HPLC assay

2003 ◽  
Vol 31 (2) ◽  
pp. 283-289 ◽  
Author(s):  
L.Z Wang ◽  
P.C Ho ◽  
H.S Lee ◽  
H.K Vaddi ◽  
Y.W Chan ◽  
...  
Keyword(s):  
Rat Plasma ◽  
Reversed Phase ◽  
Hplc Assay ◽  
Reversed Phase Hplc

scholarly journals A Gastrointestinal Transit Study on Amphotericin B-Loaded Solid Lipid Nanoparticles in Rats

2015 ◽  
Vol 16 (4) ◽  
pp. 871-877 ◽  
Author(s):  
Hilda Amekyeh ◽  
Nashiru Billa ◽  
Kah-Hay Yuen ◽  
Sherlyn Lim Sheau Chin
Keyword(s):  
Amphotericin B ◽  
Solid Lipid Nanoparticles ◽  
Gastrointestinal Transit ◽  
Lipid Nanoparticles ◽  
Solid Lipid ◽  
Transit Study
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