Bioanalysis of free and liposomal Amphotericin B in rat plasma using solid phase extraction and protein precipitation followed by LC-MS/MS

2018 ◽  
Vol 158 ◽  
pp. 288-293 ◽  
Author(s):  
Chong Su ◽  
Hong Yang ◽  
Hui Sun ◽  
John Paul Fawcett ◽  
Dong Sun ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Amphotericin B ◽  
Liposomal Amphotericin ◽  
Solid Phase ◽  
Rat Plasma ◽  
Protein Precipitation ◽  
Liposomal Amphotericin B ◽  
Phase Extraction

Rapid and simple determination of nicorandil in rat plasma using a solid-phase extraction column

1990 ◽  
Vol 528 ◽  
pp. 509-516 ◽  
Author(s):  
Yutaka Gomita ◽  
Katsushi Furuno ◽  
Kohei Eto ◽  
Tamotsu Fukuda ◽  
Yasunori Araki ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Rat Plasma ◽  
Extraction Column ◽  
Phase Extraction ◽  
Solid Phase Extraction Column

scholarly journals A Modified Method for Determination of Lumefantrine in Human Plasma by HPLC-UV and Combination of Protein Precipitation and Solid-Phase Extraction: Application to a Pharmacokinetic Study

2010 ◽  
Vol 5 ◽  
pp. ACI.S4431 ◽  
Author(s):  
Liusheng Huang ◽  
Patricia S. Lizak ◽  
Anura L. Jayewardene ◽  
Florence Marzan ◽  
Ming-Na Tina Lee ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Human Plasma ◽  
Solid Phase ◽  
Internal Standard ◽  
Pharmacokinetic Interaction ◽  
Gradient Elution ◽  
Protein Precipitation ◽  
Phase Extraction ◽  
Modified Method

An HPLC-UV method was developed and validated for the determination of lumefantrine in human plasma. Lumefantrine and its internal standard halofantrine were extracted from plasma samples using protein precipitation with acetonitrile (0.2% perchloric acid) followed by solid-phase extraction with Hypersep C8 cartridges. Chromatographic separation was performed on a Zorbax SB-CN HPLC column (3.0 × 150 mm, 3.5 μm) with water/methanol (0.1% TFA) as the mobile phases in a gradient elution mode. Detection was performed using UV/vis detector at λ = 335 nm. The method showed to be linear over a range of 50-10,000 ng/mL with acceptable intra- and inter-day precision and accuracy. The mean recoveries were 88.2% for lumefatrine and 84.5% for the I.S. The internal standard halofantrine is readily available from commercial sources. This method was successfully applied to a pharmacokinetic interaction study between a first-line antimalarial combination (artemether—lumefantrine) and antiretroviral therapy.

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