scholarly journals Targeted Killing of Streptococcus mutans in Biofilms by a Pheromone Guided Antimicrobial Peptide HP30

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Tian XL ◽  
Chen C ◽  
Cyr K ◽  
Dong G ◽  
Salim H ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Streptococcus Mutans ◽  
Targeted Killing

scholarly journals Targeted Killing of Streptococcus mutans by a Pheromone-Guided “Smart” Antimicrobial Peptide

2006 ◽  
Vol 50 (11) ◽  
pp. 3651-3657 ◽  
Author(s):  
Randal Eckert ◽  
Jian He ◽  
Daniel K. Yarbrough ◽  
Fengxia Qi ◽  
Maxwell H. Anderson ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Streptococcus Mutans ◽  
Targeted Delivery ◽  
Wide Spectrum ◽  
Oral Streptococci ◽  
Normal Flora ◽  
Targeted Killing ◽  
Amino Acid Region ◽  
Clinical Consequences ◽  
Species Specific

ABSTRACT Within the repertoire of antibiotics available to a prescribing clinician, the majority affect a broad range of microorganisms, including the normal flora. The ecological disruption resulting from antibiotic treatment frequently results in secondary infections or other negative clinical consequences. To address this problem, our laboratory has recently developed a new class of pathogen-selective molecules, called specifically (or selectively) targeted antimicrobial peptides (STAMPs), based on the fusion of a species-specific targeting peptide domain with a wide-spectrum antimicrobial peptide domain. In the current study, we focused on achieving targeted killing of Streptococcus mutans, a cavity-causing bacterium that resides in a multispecies microbial community (dental plaque). In particular, we explored the possibility of utilizing a pheromone produced by S. mutans, namely, the competence stimulating peptide (CSP), as a STAMP targeting domain to mediate S. mutans-specific delivery of an antimicrobial peptide domain. We discovered that STAMPs constructed with peptides derived from CSP were potent against S. mutans grown in liquid or biofilm states but did not affect other oral streptococci tested. Further studies showed that an 8-amino-acid region within the CSP sequence is sufficient for targeted delivery of the antimicrobial peptide domain to S. mutans. The STAMPs presented here are capable of eliminating S. mutans from multispecies biofilms without affecting closely related noncariogenic oral streptococci, indicating the potential of these molecules to be developed into “probiotic” antibiotics which could selectively eliminate pathogens while preserving the protective benefits of a healthy normal flora.

scholarly journals Antimicrobial peptide GH12 targets Streptococcus mutans to arrest caries development in rats

2018 ◽  
Vol 11 (1) ◽  
pp. 1549921 ◽  
Author(s):  
Yufei Wang ◽  
Yuhao Zeng ◽  
Yuanjing Wang ◽  
Haoran Li ◽  
Sihan Yu ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Streptococcus Mutans

scholarly journals Clinical Efficacy of a Specifically Targeted Antimicrobial Peptide Mouth Rinse: Targeted Elimination of Streptococcus mutans and Prevention of Demineralization

2011 ◽  
Vol 45 (5) ◽  
pp. 415-428 ◽  
Author(s):  
R. Sullivan ◽  
P. Santarpia ◽  
S. Lavender ◽  
E. Gittins ◽  
Z. Liu ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Streptococcus Mutans ◽  
Clinical Efficacy ◽  
Mouth Rinse

scholarly journals Antimicrobial Activity of Protamine-Loaded Calcium Phosphates against Oral Bacteria

Materials ◽  
2019 ◽  
Vol 12 (17) ◽  
pp. 2816 ◽  
Author(s):  
Masashi Fujiki ◽  
Kodai Abe ◽  
Tohru Hayakawa ◽  
Takatsugu Yamamoto ◽  
Mana Torii ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Peptide ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Calcium Phosphates ◽  
Oral Bacteria ◽  
The Other ◽  
Dental Material ◽  
Planktonic Bacteria ◽  
Surrounding Environment

Protamine is an antimicrobial peptide extracted from fish. In this study, we loaded protamine onto dicalcium phosphate anhydride (DCPA), a dental material. Protamine was loaded by stirring DCPA into a protamine solution. To explore the antimicrobial activity of the materials, we cultivated Streptococcus mutans on fabricated discs for 24 h. When S. mutans was cultivated on the discs under no sucrose conditions, the loaded protamine was not released, and the ratio of dead bacteria increased on the surface of P (125) DCPA (half of the saturated level of protamine (125 ppm protamine) was loaded). Aside from P (500) DCPA (saturated level of protamine was loaded), some protamine was released, and the number of planktonic bacteria in the supernatant decreased. Using medium containing 1% sucrose, the release of protamine was promoted from P (125) DCPA due to lowered pH. However, lowering of the pH decreased the antimicrobial activity of protamine. On the other hand, P (500) DCPA released protamine before the pH was lowered, and biofilm formation was inhibited. The loaded protamine expressed antimicrobial activity, both on the surface of the materials and in the surrounding environment. The interaction of loaded protamine with calcium phosphates could promote the application of protamine in the dental field.

Effect of a novel antimicrobial peptide chrysophsin-1 on oral pathogens and Streptococcus mutans biofilms

Peptides ◽  
2012 ◽  
Vol 33 (2) ◽  
pp. 212-219 ◽  
Author(s):  
Wei Wang ◽  
Rui Tao ◽  
Zhongchun Tong ◽  
Yonglin Ding ◽  
Rong Kuang ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Streptococcus Mutans ◽  
Oral Pathogens

scholarly journals Antibacterial activity of a novel antimicrobial peptide [W7]KR12-KAEK derived from KR-12 against Streptococcus mutans planktonic cells and biofilms

Biofouling ◽  
2017 ◽  
Vol 33 (10) ◽  
pp. 835-846 ◽  
Author(s):  
B. R. da Silva ◽  
A. J. S. Conrado ◽  
A. L. Pereira ◽  
F. F. V. Evaristo ◽  
F. V. S. Arruda ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antimicrobial Peptide ◽  
Streptococcus Mutans ◽  
Planktonic Cells

scholarly journals Effects of Antimicrobial Peptide GH12 on the Cariogenic Properties and Composition of a Cariogenic Multispecies Biofilm

2018 ◽  
Vol 84 (24) ◽  
Author(s):  
Wentao Jiang ◽  
Yufei Wang ◽  
Junyuan Luo ◽  
Xinwei Li ◽  
Xuedong Zhou ◽  
...  
Keyword(s):  
Dental Caries ◽  
Antimicrobial Peptide ◽  
Streptococcus Mutans ◽  
Lactic Acid Production ◽  
Minimal Bactericidal Concentration ◽  
Content Type ◽  
Biofilm Model ◽  
Planktonic Bacteria ◽  
Multispecies Biofilm ◽  
Glucan Synthesis

ABSTRACTDental caries is a biofilm-mediated disease that occurs when acidogenic/aciduric bacteria obtain an ecological advantage over commensal species. In previous studies, the effects of the antimicrobial peptide GH12 on planktonic bacteria and monospecies biofilms were confirmed. The objectives of this study were to investigate the effects of GH12 on a cariogenic multispecies biofilm and to preliminarily explain the mechanism. In this biofilm model,Streptococcus mutansATCC 70061 was the representative of cariogenic bacteria, whileStreptococcus gordoniiATCC 35105 andStreptococcus sanguinisJCM 5708 were selected as healthy microbiota. The results showed that GH12 was more effective in suppressingS. mutansthan the other two species, with lower MIC and minimal bactericidal concentration (MBC) values among diverse type strains and clinical isolated strains. Therefore, GH12, at no more than 8 mg/liter, was used to selectively suppressS. mutansin the multispecies biofilm. GH12 at 4 mg/liter and 8 mg/liter reduced the cariogenic properties of the multispecies biofilm in biofilm formation, glucan synthesis, and lactic acid production. In addition, GH12 suppressedS. mutanswithin the multispecies biofilm and changed the bacterial composition. Furthermore, 8 mg/liter GH12 showed a selective bactericidal impact onS. mutans, and GH12 promoted hydrogen peroxide production inS. sanguinisandS. gordonii, which improved their ecological advantages. In conclusion, GH12 inhibited the cariogenic properties and changed the composition of the multispecies biofilm through a two-part mechanism by which GH12 directly suppressed the growth ofS. mutansas well as enhanced the ecological competitiveness ofS. sanguinisandS. gordonii.IMPORTANCEDental caries is one of the most prevalent chronic infectious diseases worldwide, with substantial economic and quality-of-life impacts.Streptococcus mutanshas been considered the principal pathogen of dental caries. To combat dental caries, an antimicrobial peptide, GH12, was designed, and its antibacterial effects on planktonicS. mutansand the monospecies biofilm were confirmed. As etiological concepts of dental caries evolved to include microecosystems, the homeostasis between pathogenic and commensal bacteria and a selective action on cariogenic virulence have increasingly become the focus. The novelty of this research was to study the effects of the antimicrobial peptides on a controlled cariogenic multispecies biofilm model. Notably, the role of an antimicrobial agent in regulating interspecific competition and composition shifts within this multispecies biofilm was investigated. With promising antibacterial and antibiofilm properties, the use of GH12 might be of importance in preventing and controlling caries and other dental infections.

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