scholarly journals Coordinate responses to alkaline pH stress in budding yeast

2015 ◽  
Vol 2 (6) ◽  
pp. 182-196 ◽  
Author(s):  
Albert Serra-Cardona ◽  
David Canadell ◽  
Joaquin Arino
Keyword(s):  
Budding Yeast ◽  
Alkaline Ph ◽  
Ph Stress ◽  
Alkaline Ph Stress

scholarly journals Signaling Alkaline pH Stress in the YeastSaccharomyces cerevisiaethrough the Wsc1 Cell Surface Sensor and the Slt2 MAPK Pathway

2006 ◽  
Vol 281 (52) ◽  
pp. 39785-39795 ◽  
Author(s):  
Raquel Serrano ◽  
Humberto Martín ◽  
Antonio Casamayor ◽  
Joaquín Ariño
Keyword(s):  
Cell Surface ◽  
Mapk Pathway ◽  
Alkaline Ph ◽  
Ph Stress ◽  
Alkaline Ph Stress

The role of the Snf1 kinase in the adaptive response of Saccharomyces cerevisiae to alkaline pH stress

2012 ◽  
Vol 444 (1) ◽  
pp. 39-49 ◽  
Author(s):  
Antonio Casamayor ◽  
Raquel Serrano ◽  
María Platara ◽  
Carlos Casado ◽  
Amparo Ruiz ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Glucose Sensing ◽  
Limiting Factors ◽  
Alkaline Ph ◽  
Short Term ◽  
Triple Mutant ◽  
Snf1 Kinase ◽  
High Ph ◽  
Ph Stress ◽  
Alkaline Ph Stress

Alkaline pH stress invokes a potent and fast transcriptional response in Saccharomyces cerevisiae that includes many genes repressed by glucose. Certain mutants in the glucose-sensing and -response pathways, such as those lacking the Snf1 kinase, are sensitive to alkalinization. In the present study we show that the addition of glucose to the medium improves the growth of wild-type cells at high pH, fully abolishes the snf1 alkali-sensitive phenotype and attenuates high pH-induced Snf1 phosphorylation at Thr210. Lack of Elm1, one of the three upstream Snf1 kinases (Tos3, Elm1 and Sak1), markedly increases alkali sensitivity, whereas the phenotype of the triple mutant tos3 elm1 sak1 is even more pronounced than that of snf1 cells and is poorly rescued by glucose supplementation. DNA microarray analysis reveals that about 75% of the genes induced in the short term by high pH are also induced by glucose scarcity. Snf1 mediates, in full or in part, the activation of a significant subset (38%) of short-term alkali-induced genes, including those encoding high-affinity hexose transporters and phosphorylating enzymes. The induction of genes encoding enzymes involved in glycogen, but not trehalose, metabolism is largely dependent of the presence of Snf1. Therefore the function of Snf1 in adaptation to glucose scarcity appears crucial for alkaline pH tolerance. Incorporation of micromolar amounts of iron and copper to a glucose-supplemented medium resulted in an additive effect and allows near-normal growth at high pH, thus indicating that these three nutrients are key limiting factors for growth in an alkaline environment.

Genomic stability disorders from budding yeast to humans

10.2741/s379 ◽  
2013 ◽  
Vol S5 (2) ◽  
pp. 396-411 ◽  
Author(s):  
Nicolas Carlos Hoch
Keyword(s):  
Budding Yeast ◽  
Genomic Stability
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