scholarly journals Phospho-regulation and nucleocytoplasmic trafficking of CrzA in response to calcium and alkaline-pH stress inAspergillus nidulans

2013 ◽  
Vol 89 (3) ◽  
pp. 532-551 ◽  
Author(s):  
Patricia Hernández-Ortiz ◽  
Eduardo A. Espeso
2006 ◽  
Vol 281 (52) ◽  
pp. 39785-39795 ◽  
Author(s):  
Raquel Serrano ◽  
Humberto Martín ◽  
Antonio Casamayor ◽  
Joaquín Ariño

2012 ◽  
Vol 444 (1) ◽  
pp. 39-49 ◽  
Author(s):  
Antonio Casamayor ◽  
Raquel Serrano ◽  
María Platara ◽  
Carlos Casado ◽  
Amparo Ruiz ◽  
...  

Alkaline pH stress invokes a potent and fast transcriptional response in Saccharomyces cerevisiae that includes many genes repressed by glucose. Certain mutants in the glucose-sensing and -response pathways, such as those lacking the Snf1 kinase, are sensitive to alkalinization. In the present study we show that the addition of glucose to the medium improves the growth of wild-type cells at high pH, fully abolishes the snf1 alkali-sensitive phenotype and attenuates high pH-induced Snf1 phosphorylation at Thr210. Lack of Elm1, one of the three upstream Snf1 kinases (Tos3, Elm1 and Sak1), markedly increases alkali sensitivity, whereas the phenotype of the triple mutant tos3 elm1 sak1 is even more pronounced than that of snf1 cells and is poorly rescued by glucose supplementation. DNA microarray analysis reveals that about 75% of the genes induced in the short term by high pH are also induced by glucose scarcity. Snf1 mediates, in full or in part, the activation of a significant subset (38%) of short-term alkali-induced genes, including those encoding high-affinity hexose transporters and phosphorylating enzymes. The induction of genes encoding enzymes involved in glycogen, but not trehalose, metabolism is largely dependent of the presence of Snf1. Therefore the function of Snf1 in adaptation to glucose scarcity appears crucial for alkaline pH tolerance. Incorporation of micromolar amounts of iron and copper to a glucose-supplemented medium resulted in an additive effect and allows near-normal growth at high pH, thus indicating that these three nutrients are key limiting factors for growth in an alkaline environment.


2015 ◽  
Vol 2 (6) ◽  
pp. 182-196 ◽  
Author(s):  
Albert Serra-Cardona ◽  
David Canadell ◽  
Joaquin Arino

2011 ◽  
Vol 35 (2) ◽  
pp. 256-261
Author(s):  
Wen-Xiang LI ◽  
Jun XIE ◽  
Rui SONG ◽  
Hong ZOU ◽  
Shan-Gong WU ◽  
...  

2013 ◽  
Vol 18 (3) ◽  
pp. 556-564 ◽  
Author(s):  
Yun WANG ◽  
Jian LI ◽  
Jitao LI ◽  
Yuying HE ◽  
Zhiqiang CHANG ◽  
...  

1985 ◽  
Vol 17 (10) ◽  
pp. 219-227 ◽  
Author(s):  
T. Omura ◽  
H. K. Shin ◽  
A. Ketratanakul

Coliphages are among the most promising indicators of viral inactivation efficiency of wastewater treatment. Therefore, it is important to investigate the behaviour of coliphages in oxidation ponds from the viewpoint of predicting the inactivation of infectious viruses. In this study, numbers of coliphages were measured in oxidation ponds consisting of a series of facultative and maturation ponds. In parallel with this investigation, the effects of temperature and pH on the behaviour of coliphages were examined in the laboratory, employing three species of coliform bacteria as host cells. The field investigation showed that there was positive correlation between counts of coliphages and those of coliform bacteria, and that more than 99% of coliphages were inactivated. The inactivation efficiency of coliphages in the facultative pond was much higher than in the maturation pond. The results of the laboratory experiments indicated that at 30°C more than 99% of the coliform group were destroyed in 7 days of incubation and that coliphages counts increased from 105/100 ml to 107/100 ml with a lag time of 3 days. Greater reduction of the coliform count was obtained at higher incubation temperatures. It was observed that the coliphages possessed greater ability to attack coliform bacteria at acidic rather than alkaline pH.


2015 ◽  
Vol 22 (12) ◽  
pp. 1066-1075 ◽  
Author(s):  
Adriana Magalhães ◽  
Rayner Queiroz ◽  
Izabela Bastos ◽  
Jaime Santana ◽  
Marcelo Sousa ◽  
...  

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