Disposable Smart Plastic Biochips For Clinical Diagnostics

2002 ◽  
Vol 729 ◽  
Author(s):  
Chong H. Ahn ◽  
Jin-Woo Choi ◽  
Sanghyo Kim ◽  
Young-Soo Sohn ◽  
Aniruddha Puntambekar ◽  
...  
Keyword(s):  
Passive Control ◽  
Point Of Care ◽  
Low Cost ◽  
Microfluidic System ◽  
Clinical Diagnostics ◽  
Plastic Substrate ◽  
Driving Mechanisms ◽  
Care Health ◽  
Diagnostic Applications ◽  
On Chip

AbstractThis paper presents an overview of the development of novel disposable smart plastic fluidic biochips for clinical diagnostic applications. The biochip is manufactured using a low-cost, rapid turn around injection molding/embossing process on a plastic substrate. The plastic fluidic biochip uses a novel sPROMs (structurally programmable microfluidic system) approach to achieve passive control of fluidic sequencing [1-2]. The plastic biochip also uses an on-chip pressurized air source for fluidic movement thus eliminating the need for active driving mechanisms and allowing for a truly disposable approach. Furthermore, electrochemical biosensors are also integrated on-chip to analyze various metabolically significant parameters such as PO2(partial pressure of oxygen), Glucose, Lactate,and pH. The fluidic biochip is being developed for point-of-care health monitoring applications where parameters such as small size, simplicity of operation, disposability, reduced cross-contamination are vital. The issues mentioned above are successfully addressed using the approach of this work and are discussed in this paper.

scholarly journals DESIGN AND CONSTRUCTION OF A LAB-ON-A-PAPER FOR LOW-COST AND DISPOSABLE POINT-OF CARE DIAGNOSTICS

2017 ◽  
Vol 5 (1) ◽  
pp. 26
Author(s):  
Afnidar Afnidar ◽  
Bambang Kuswandi
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Diagnostic System ◽  
Microfluidic System ◽  
Clinical Diagnostics ◽  
Detection Zone ◽  
Power Sources ◽  
Point Of Care Diagnostics ◽  
Paper Based Microfluidics ◽  
Analysis System

Abstract This paper presents a low-cost and disposable paper based microfluidic analysis system for point-of-care diagnostics. Detection is achieved by using a colorimetric or visual indicator. Immobilized specific reagent or enzymes designed for the parameter under consideration act as capture molecules on the surface of the detection zone. The sensor is integrated into a microfluidic system made of paper (cellulose). An additional component of the analysis system is a capillary unit which is used to introduce the analyte to the detection zone. For this purpose well- defined, millimeter-sized channel, comprising hydrophobic polymer bounded onto hydrophilic paper was created. Then the detection zone was coated with a sensitive reagent layer as a sensor region. The paper based microfluidics also called lab on paper, has been fabricated using screen printing technology as the basis for low-cost, disposable, portable and technically simple fabrication for mass production. Microfluidics in paper make it feasible to run single, dual or even multiple clinical analyses on one strip of paper while still using only small volumes of a single sample. The capability of lab on paper for detection of importance clinical analyte protein in urine, saliva and blood samples has been demonstrate successfully. Lab on paper as a diagnostic system is small, disposable, and easy to use and requires no external equipment, reagents, or power sources. This kind of diagnostic system is attractive for use in developing countries, in the field, or as a low-cost alternative to more-advanced technologies already used in clinical diagnostics. Keywords: Lab-on-a-paper, Point-of-care, Visual detection, Clinical diagnostic, Disposable sensor

scholarly journals Design and Manufacturing of a Disposable, Cyclo-Olefin Copolymer, Microfluidic Device for a Biosensor †

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 1178 ◽  
Author(s):  
Jorge Prada ◽  
Christina Cordes ◽  
Carsten Harms ◽  
Walter Lang
Keyword(s):  
Microfluidic Device ◽  
Heating Temperature ◽  
Low Cost ◽  
Reaction Chamber ◽  
Microfluidic System ◽  
Heating Process ◽  
Design And Manufacturing ◽  
On Chip ◽  
Working Parameters ◽  
Auto Fluorescence

This contribution outlines the design and manufacturing of a microfluidic device implemented as a biosensor for retrieval and detection of bacteria RNA. The device is fully made of Cyclo-Olefin Copolymer (COC), which features low auto-fluorescence, biocompatibility and manufacturability by hot-embossing. The RNA retrieval was carried on after bacteria heat-lysis by an on-chip micro-heater, whose function was characterized at different working parameters. Carbon resistive temperature sensors were tested, characterized and printed on the biochip sealing film to monitor the heating process. Off-chip and on-chip processed RNA were hybridized with capture probes on the reaction chamber surface and identification was achieved by detection of fluorescence tags. The application of the mentioned techniques and materials proved to allow the development of low-cost, disposable albeit multi-functional microfluidic system, performing heating, temperature sensing and chemical reaction processes in the same device. By proving its effectiveness, this device contributes a reference to show the integration potential of fully thermoplastic devices in biosensor systems.

scholarly journals Rapid and Sensitive Detection of miRNA Based on AC Electrokinetic Capacitive Sensing for Point-of-Care Applications

Sensors ◽  
2021 ◽  
Vol 21 (12) ◽  
pp. 3985
Author(s):  
Nan Wan ◽  
Yu Jiang ◽  
Jiamei Huang ◽  
Rania Oueslati ◽  
Shigetoshi Eda ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Clinical Diagnostics ◽  
Detection Methods ◽  
Capacitive Sensing ◽  
Application Potential ◽  
Mirna Detection ◽  
Mirna 25 ◽  
Low Sensitivity

A sensitive and efficient method for microRNAs (miRNAs) detection is strongly desired by clinicians and, in recent years, the search for such a method has drawn much attention. There has been significant interest in using miRNA as biomarkers for multiple diseases and conditions in clinical diagnostics. Presently, most miRNA detection methods suffer from drawbacks, e.g., low sensitivity, long assay time, expensive equipment, trained personnel, or unsuitability for point-of-care. New methodologies are needed to overcome these limitations to allow rapid, sensitive, low-cost, easy-to-use, and portable methods for miRNA detection at the point of care. In this work, to overcome these shortcomings, we integrated capacitive sensing and alternating current electrokinetic effects to detect specific miRNA-16b molecules, as a model, with the limit of detection reaching 1.0 femto molar (fM) levels. The specificity of the sensor was verified by testing miRNA-25, which has the same length as miRNA-16b. The sensor we developed demonstrated significant improvements in sensitivity, response time and cost over other miRNA detection methods, and has application potential at point-of-care.

Point-of-Care Systems for Rapid DNA Quantification in Oncology

2008 ◽  
Vol 94 (2) ◽  
pp. 216-225 ◽  
Author(s):  
Marco Bianchessi ◽  
Sarah Burgarella ◽  
Marco Cereda
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Semiconductor Industry ◽  
Point Of Care Testing ◽  
Lab On Chip ◽  
Diagnostic Assays ◽  
Care Systems ◽  
Point Of Care Systems ◽  
The Common ◽  
On Chip

The development of new powerful applications and the improvement in fabrication techniques are promising an explosive growth in lab-on-chip use in the upcoming future. As the demand reaches significant levels, the semiconductor industry may enter in the field, bringing its capability to produce complex devices in large volumes, high quality and low cost. The lab-on-chip concept, when applied to medicine, leads to the point-of-care concept, where simple, compact and cheap instruments allow diagnostic assays to be performed quickly by untrained personnel directly at the patient's side. In this paper, some practical and economical considerations are made to support the advantages of point-of-care testing. A series of promising technologies developed by STMicroelectronics on lab-on-chips is also presented, mature enough to enter in the common medical practice. The possible use of these techniques for cancer research, diagnosis and treatment are illustrated together with the benefits offered by their implementation in point-of-care testing.

scholarly journals Design and Manufacturing of a Disposable, Cyclo-Olefin Copolymer, Microfluidic Biosensor

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 810
Author(s):  
Jorge Prada ◽  
Christina Cordes ◽  
Carsten Harms ◽  
Walter Lang
Keyword(s):  
Heating Temperature ◽  
Low Cost ◽  
Reaction Chamber ◽  
Microfluidic System ◽  
Heating Process ◽  
Design And Manufacturing ◽  
Microfluidic Biosensor ◽  
On Chip ◽  
Auto Fluorescence ◽  
Reaction Processes

This contribution outlines the design and manufacturing of a biosensor for retrieval and detection of bacteria RNA. The device is fully made of Cyclo-Olefin Copolymer (COC), which features low auto-fluorescence, biocompatibility and manufacturability by hot-embossing. The RNA retrieval is carried on after bacteria heat-lysis by an on-chip micro-heater. Two additional carbon resistive temperature sensors printed on the biochip sealing film monitor the heating process. RNA is hybridized with capture probes on the reaction chamber surface and identification is achieved by detection of fluorescence tags. The application of the mentioned techniques and materials facilitates the development of low-cost, disposable albeit multi-functional microfluidic system, performing heating, temperature sensing and chemical reaction processes in the same device. By proving its effectiveness, this device contributes a reference to show the potential of fully thermoplastic devices as biosensors.

scholarly journals Whole Blood Immunoassay Based on Centrifugal Bead Sedimentation

2011 ◽  
Vol 57 (5) ◽  
pp. 753-761 ◽  
Author(s):  
Ulrich Y Schaff ◽  
Greg J Sommer
Keyword(s):  
Whole Blood ◽  
Single Channel ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Microfluidic System ◽  
Blood Samples ◽  
Reactive Protein ◽  
Immunoassay Technique ◽  
Microfluidic Immunoassay

BACKGROUND Centrifugal “lab on a disk” microfluidics is a promising avenue for developing portable, low-cost, automated immunoassays. However, the necessity of incorporating multiple wash steps results in complicated designs that increase the time and sample/reagent volumes needed to run assays and raises the probability of errors. We present proof of principle for a disk-based microfluidic immunoassay technique that processes blood samples without conventional wash steps. METHODS Microfluidic disks were fabricated from layers of patterned, double-sided tape and polymer sheets. Sample was mixed on-disk with assay capture beads and labeling antibodies. Following incubation, the assay beads were physically separated from the blood cells, plasma, and unbound label by centrifugation through a density medium. A signal-laden pellet formed at the periphery of the disk was analyzed to quantify concentration of the target analyte. RESULTS To demonstrate this technique, the inflammation biomarkers C-reactive protein and interleukin-6 were measured from spiked mouse plasma and human whole blood samples. On-disk processing (mixing, labeling, and separation) facilitated direct assays on 1-μL samples with a 15-min sample-to-answer time, <100 pmol/L limit of detection, and 10% CV. We also used a unique single-channel multiplexing technique based on the sedimentation rate of different size or density bead populations. CONCLUSIONS This portable microfluidic system is a promising method for rapid, inexpensive, and automated detection of multiple analytes directly from a drop of blood in a point-of-care setting.

scholarly journals Biosensors-on-Chip: An Up-to-Date Review

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 6013
Author(s):  
Cristina Chircov ◽  
Alexandra Cătălina Bîrcă ◽  
Alexandru Mihai Grumezescu ◽  
Ecaterina Andronescu
Keyword(s):  
Neurodegenerative Disorders ◽  
System Development ◽  
Point Of Care ◽  
Quantitative Information ◽  
Recent Application ◽  
Physical Chemical ◽  
Target Analytes ◽  
Diagnosis Of Cancer ◽  
Diagnostic Applications ◽  
On Chip

Generally, biosensors are designed to translate physical, chemical, or biological events into measurable signals, thus offering qualitative and/or quantitative information regarding the target analytes. While the biosensor field has received considerable scientific interest, integrating this technology with microfluidics could further bring significant improvements in terms of sensitivity and specificity, resolution, automation, throughput, reproducibility, reliability, and accuracy. In this manner, biosensors-on-chip (BoC) could represent the bridging gap between diagnostics in central laboratories and diagnostics at the patient bedside, bringing substantial advancements in point-of-care (PoC) diagnostic applications. In this context, the aim of this manuscript is to provide an up-to-date overview of BoC system development and their most recent application towards the diagnosis of cancer, infectious diseases, and neurodegenerative disorders.

On–Chip Droplets Sensing using Capacitive Technique

2013 ◽  
Vol 61 (2) ◽  
Author(s):  
Mohamad Faizal Abdullah ◽  
P. L. Leow ◽  
M. A. Abd Razak ◽  
F. K. Che Harun
Keyword(s):  
Low Cost ◽  
Microfluidic Channel ◽  
High Sensitivity ◽  
Microfluidic Devices ◽  
Capacitive Sensor ◽  
Sample Volume ◽  
Microfluidic System ◽  
Coplanar Electrodes ◽  
On Chip ◽  
Significant Attention

Significant attention has been given on the development of droplets–based microfluidic system because of its potential and apparent advantages. Beside the advantages of reducing the sample volume, it’s also offer less time consuming for the analysis. Optical and fluorescence among the famous method that was used in detection of droplets but they are normally bulky, expensive and not easily accessed. This paper proposed a simple, low cost and high sensitivity for droplets sensing in microfluidic devices by using capacitive sensor. Coplanar electrodes are used to form a capacitance through the microfluidic channel. The design of eight pair of electrodes was used to detect the presence of a droplet. Changes in capacitance due to the presence of a droplet in the sensing area is detected and used to trigger the microscope to capture the image of detected droplets in microchannel. The measurement of droplets detected and counting are displayed through a LABVIEW interface in the real time.

scholarly journals Developments in Transduction, Connectivity and AI/Machine Learning for Point-of-Care Testing

Sensors ◽  
2019 ◽  
Vol 19 (8) ◽  
pp. 1917 ◽  
Author(s):  
Shane O’Sullivan ◽  
Zulfiqur Ali ◽  
Xiaoyi Jiang ◽  
Reza Abdolvand ◽  
M Selim Ünlü ◽  
...  
Keyword(s):  
Machine Learning ◽  
Data Analytics ◽  
Point Of Care ◽  
Developed Countries ◽  
Clinical Diagnostics ◽  
Least Developed Countries ◽  
Point Of Care Testing ◽  
Middle Income ◽  
Lab On Chip ◽  
On Chip

We review some emerging trends in transduction, connectivity and data analytics for Point-of-Care Testing (POCT) of infectious and non-communicable diseases. The patient need for POCT is described along with developments in portable diagnostics, specifically in respect of Lab-on-chip and microfluidic systems. We describe some novel electrochemical and photonic systems and the use of mobile phones in terms of hardware components and device connectivity for POCT. Developments in data analytics that are applicable for POCT are described with an overview of data structures and recent AI/Machine learning trends. The most important methodologies of machine learning, including deep learning methods, are summarised. The potential value of trends within POCT systems for clinical diagnostics within Lower Middle Income Countries (LMICs) and the Least Developed Countries (LDCs) are highlighted.

scholarly journals A Single-Bead-Based, Fully Integrated Microfluidic System for High-Throughput CD4+T Lymphocyte Enumeration

2017 ◽  
Vol 23 (2) ◽  
pp. 134-143 ◽  
Author(s):  
Xianbo Qiu ◽  
Junhui Zhang ◽  
Shisong Gong ◽  
Dong Wang ◽  
Shan Qiao ◽  
...  
Keyword(s):  
T Lymphocyte ◽  
High Throughput ◽  
Point Of Care ◽  
Low Cost ◽  
Ccd Camera ◽  
Microfluidic System ◽  
Magnetic Control ◽  
Magnetic Actuation ◽  
Fully Integrated ◽  
Single Bead

A single-bead-based, fully integrated microfluidic system has been developed for high-throughput CD4+T lymphocyte enumeration at point-of-care testing. Instead of directly counting CD4+T lymphocytes, CD4+T lymphocyte enumeration is achieved by quantitatively detecting CD4 antigen from the lysed blood sample with a functionalized polycarbonate single bead based on chemiluminescence. To implement the sandwiched chemiluminescence immunoassay with reduced nonspecific binding, a streamlined microfluidic chip with multiple reaction chambers is developed to allow each reaction step to be completed in an independent chamber where reagent is pre-stored. With simple magnetic control, the single bead with an embedded ferrous core can be consecutively transported between each of two adjacent chambers for different reactions. Meanwhile, enhanced mixing can be achieved by moving the single bead back and forth inside one chamber with magnetic actuation. High-throughput detection can be performed when a linear actuation stage is adopted to introduce synchronous magnetic control to multiple single beads in parallel microfluidic chips. A sensitive charge-coupled device (CCD) camera is adopted for high-throughput chemiluminescence detection from multiple single beads. Experimental results show that with the fully integrated microfluidic system, easy-to-operate, accurate, low-cost, immediate, and high-throughput CD4+T lymphocyte enumeration can be successfully achieved at resource-poor settings.

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