Formation of Two-Dimensional Crystals of Membrane-Anchored and Water-Soluble Proteins

1993 ◽  
Vol 330 ◽  
Author(s):  
Harald Engelhardt ◽  
T. Scheybani ◽  
W. Von Gustedt ◽  
W. Baumeister
Keyword(s):  
Molecular Sieves ◽  
Soluble Proteins ◽  
Water Soluble ◽  
Biological Macromolecules ◽  
Complex Structures ◽  
Two Dimensional ◽  
Protein Species ◽  
Future Developments ◽  
Multiple Protein ◽  
Support Devices

ABSTRACTThe formation of two-dimensional (2-D) crystals of biological macromolecules is of interest for nanotechnological applications. Protein 2-D crystals may be used as molecular sieves and/or support devices as components of biosensors etc. [1]. Functionally specialized 2-D crystals, containing transport or catalytic proteins, provide a certain function in a highly efficient and vectorial manner. Future developments may allow the design of more complex structures such as multilayers made from different proteins or arrays of functionally linked oligo- or multimeric complexes consisting of multiple protein species [2]. Regular 2-D arrays, either truely crystalline or densely packed molecules, are one of the basic structures taht might be used to construct more sophisticated protein-based devices. 2-D crystals have some interesting features:

Mesoporous Silica Molecular Sieve based Nanocarriers: Transpiring Drug Dissolution Research

2017 ◽  
Vol 23 (3) ◽  
pp. 467-480 ◽  
Author(s):  
Satyanarayan Pattnaik ◽  
Kamla Pathak
Keyword(s):  
Drug Release ◽  
Mesoporous Silica ◽  
Molecular Sieves ◽  
Release Kinetics ◽  
Drug Loading ◽  
Scale Up ◽  
Water Soluble ◽  
Drug Dissolution ◽  
Water Soluble Drugs ◽  
Loading Methods

Background: Improvement of oral bioavailability through enhancement of dissolution for poorly soluble drugs has been a very promising approach. Recently, mesoporous silica based molecular sieves have demonstrated excellent properties to enhance the dissolution velocity of poorly water-soluble drugs. Description: Current research in this area is focused on investigating the factors influencing the drug release from these carriers, the kinetics of drug release and manufacturing approaches to scale-up production for commercial manufacture. Conclusion: This comprehensive review provides an overview of different methods adopted for synthesis of mesoporous materials, influence of processing factors on properties of these materials and drug loading methods. The drug release kinetics from mesoporous silica systems, the manufacturability and stability of these formulations are reviewed. Finally, the safety and biocompatibility issues related to these silica based materials are discussed.

scholarly journals Dietary Antiplatelets: A New Perspective on the Health Benefits of the Water-Soluble Tomato Concentrate Fruitflow®

Nutrients ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 2184
Author(s):  
Niamh O’Kennedy ◽  
Ruedi Duss ◽  
Asim K Duttaroy
Keyword(s):  
Immune Responses ◽  
Cardiovascular Health ◽  
Water Soluble ◽  
Health Conditions ◽  
Specific Reference ◽  
Clinical Settings ◽  
Future Developments ◽  
Tomato Extract ◽  
New Perspective ◽  
Exercise Induced

Our understanding of platelet functionality has undergone a sea change in the last decade. No longer are platelets viewed simply as regulators of haemostasis; they are now acknowledged to be pivotal in coordinating the inflammatory and immune responses. This expanded role for platelets brings new opportunities for controlling a range of health conditions, targeting platelet activation and their interactions with other vascular cells. Antiplatelet drugs may be of wider utility than ever expected but often cause platelet suppression too strong to be used out of clinical settings. Dietary antiplatelets represent a nutritional approach that can be efficacious while safe for general use. In this review, we discuss potential new uses for dietary antiplatelets outside the field of cardiovascular health, with specific reference to the water-soluble tomato extract Fruitflow®. Its uses in different aspects of inflammation and immune function are discussed, highlighting exercise-induced inflammation, mediating the effects of air pollution, and controlling thrombotic aspects of the immune response. Potential future developments in women’s health, erectile dysfunction, and the allergic response indicate how broad the utility of dietary antiplatelets can be.

scholarly journals A thin, deformable, high-performance supercapacitor implant that can be biodegraded and bioabsorbed within an animal body

2021 ◽  
Vol 7 (2) ◽  
pp. eabe3097
Author(s):  
Hongwei Sheng ◽  
Jingjing Zhou ◽  
Bo Li ◽  
Yuhang He ◽  
Xuetao Zhang ◽  
...  
Keyword(s):  
High Performance ◽  
Electronic Devices ◽  
Form Factors ◽  
Time Frame ◽  
Water Soluble ◽  
Two Dimensional ◽  
Medical Electronics ◽  
Thin Structure ◽  
Shed Light

It has been an outstanding challenge to achieve implantable energy modules that are mechanically soft (compatible with soft organs and tissues), have compact form factors, and are biodegradable (present for a desired time frame to power biodegradable, implantable medical electronics). Here, we present a fully biodegradable and bioabsorbable high-performance supercapacitor implant, which is lightweight and has a thin structure, mechanical flexibility, tunable degradation duration, and biocompatibility. The supercapacitor with a high areal capacitance (112.5 mF cm−2 at 1 mA cm−2) and energy density (15.64 μWh cm−2) uses two-dimensional, amorphous molybdenum oxide (MoOx) flakes as electrodes, which are grown in situ on water-soluble Mo foil using a green electrochemical strategy. Biodegradation behaviors and biocompatibility of the associated materials and the supercapacitor implant are systematically studied. Demonstrations of a supercapacitor implant that powers several electronic devices and that is completely degraded after implantation and absorbed in rat body shed light on its potential uses.

The cellular basis of corneal transparency: evidence for ‘corneal crystallins’

1999 ◽  
Vol 112 (5) ◽  
pp. 613-622 ◽  
Author(s):  
J.V. Jester ◽  
T. Moller-Pedersen ◽  
J. Huang ◽  
C.M. Sax ◽  
W.T. Kays ◽  
...  
Keyword(s):  
Cellular Level ◽  
Soluble Proteins ◽  
Water Soluble ◽  
Corneal Tissue ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Corneal Transparency ◽  
Scattering Measurements ◽  
Class 1

In vivo corneal light scattering measurements using a novel confocal microscope demonstrated greatly increased backscatter from corneal stromal fibrocytes (keratocytes) in opaque compared to transparent corneal tissue in both humans and rabbits. Additionally, two water-soluble proteins, transketolase (TKT) and aldehyde dehydrogenase class 1 (ALDH1), isolated from rabbit keratocytes showed unexpectedly abundant expression (approximately 30% of the soluble protein) in transparent corneas and markedly reduced levels in opaque scleral fibroblasts or keratocytes from hazy, freeze injured regions of the cornea. Together these data suggest that the relatively high expressions of TKT and ALDH1 contribute to corneal transparency in the rabbit at the cellular level, reminiscent of enzyme-crystallins in the lens. We also note that ALDH1 accumulates in the rabbit corneal epithelial cells, rather than ALDH3 as seen in other mammals, consistent with the taxon-specificity observed among lens enzyme-crystallins. Our results suggest that corneal cells, like lens cells, may preferentially express water-soluble proteins, often enzymes, for controlling their optical properties.

Selective Transport of Water-Soluble Proteins from Aqueous to Ionic Liquid Phase via a Temperature-Sensitive Phase Change of These Mixtures

2012 ◽  
Vol 65 (11) ◽  
pp. 1548 ◽  
Author(s):  
Yuki Kohno ◽  
Nobuhumi Nakamura ◽  
Hiroyuki Ohno
Keyword(s):  
Phase Change ◽  
Aqueous Phase ◽  
Selective Dissolution ◽  
Selective Extraction ◽  
Soluble Proteins ◽  
Water Soluble ◽  
Temperature Sensitive ◽  
Salting Out ◽  
Key Factor ◽  
The Difference

Mixtures of some ionic liquids (ILs) and water show reversible phase change between a homogeneous mixture and phase-separated state by a small change in temperature. Some water-soluble proteins have been migrated from the aqueous to the IL phase. When tetrabutylphosphonium 2,4,6-trimethylbenzenesulfonate was used as an IL, cytochrome c (Cyt.c) was found to be extracted from the water phase to the IL phase. Conversely, both horseradish peroxidase (HRP) and azurin remained in the aqueous phase. This selective extraction was comprehended to be due to the difference in solubility of these proteins in both phases. The separated aqueous phase contained a small amount of IL, which induced the salting-out of Cyt.c. On the other hand, condensed IL phase promoted the salting-in of Cyt.c. As a result, Cyt.c was preferably dissolved in the hydrated IL phase rather than aqueous phase. In the case of HRP, there was only a salting-out profile upon increasing the concentration of IL, which induced selective dissolution of HRP in the aqueous phase. These results clearly suggest that the profile of salting-out and salting-in for proteins is the key factor to facilitate the selective extraction of proteins from aqueous to the IL phase.

scholarly journals Influence of temperature processing on the constitutive and inducible resistance of rye seedlings to chloride salinization

2021 ◽  
pp. 134-140
Author(s):  
L. A. Chudinova ◽  
◽  
D. R. Yusupov ◽  
Keyword(s):  
Growth Rate ◽  
Sodium Chloride ◽  
Protective Effect ◽  
Soluble Proteins ◽  
Final Concentration ◽  
Water Soluble ◽  
Thermal Pretreatment ◽  
Thermal Hardening ◽  
Influence Of Temperature ◽  
Inducible Synthesis

We studied the growth rate of rye seedlings, as well as the dynamics of the content of soluble proteins and proline in the shoots during their adaptation to sharp (300 mM NaCl once, exposure time 9 days) and gradual (100 mM NaCl, then 100 mM NaCl after 2 days to the final concentration of 400 mM) salinity with sodium chloride in the presence or absence of thermal hardening (+40°C, 3 h). The established dy-namics of the content of proline and soluble proteins in the shoots suggests that the formation of re-sistance to salinity is determined by the high constitutive level of proline, as well as the stress-inducible synthesis of proline and water-soluble proteins. Thermal pretreatment of the seedlings stimulated their constitutive stability to a greater extent. The detected metabolic changes are obviously related to one of the possible mechanisms of the protective effect of thermal hardening on subsequent salinization.

scholarly journals Membrane Proteins Are Dramatically Less Conserved than Water-Soluble Proteins across the Tree of Life

2016 ◽  
Vol 33 (11) ◽  
pp. 2874-2884 ◽  
Author(s):  
Victor Sojo ◽  
Christophe Dessimoz ◽  
Andrew Pomiankowski ◽  
Nick Lane
Keyword(s):  
Membrane Proteins ◽  
Soluble Proteins ◽  
Tree Of Life ◽  
Water Soluble
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