Nanoprobe Diffusion in Poly(Vinyl-alcohol) Gels and Solutions: Effects of pH and Dehydration

2014 ◽  
Vol 1622 ◽  
pp. 135-145
Author(s):  
Hacène Boukari ◽  
Candida Silva ◽  
Ralph Nossal ◽  
Ferenc Horkay
Keyword(s):  
Diffusion Coefficient ◽  
Vinyl Alcohol ◽  
Correlation Spectroscopy ◽  
Poly Vinyl Alcohol ◽  
Linear Polymers ◽  
Fluorescence Correlation ◽  
Slowing Down ◽  
Effects Of Ph ◽  
Physical Interactions ◽  
Pva Solutions

ABSTRACTWe report fluorescence correlation spectroscopy (FCS) measurements of the translational diffusion of two fluorescent nanoprobes, rhodamine (R6G) and carboxytetramethylrhodamine (TAMRA), embedded in poly(vinyl alcohol) (PVA) solutions and gels. The diffusion coefficient was measured as a function of the PVA concentration and pH. Furthermore, we designed and built an optical chamber to determine the diffusion coefficient of the nanoprobes within the PVA solutions and gels subjected to controlled dehydration. We find that 1) lowering pH causes an apparent slowing down of the diffusion of the nanoprobes, 2) increase of PVA concentration and crosslink density also induce slowing down of both nanoprobes, and 3) dehydration induces systematic decrease of the diffusion of TAMRA in both solutions and gels. Taken together, these results demonstrate that transient physical interactions between the nanoprobes and the PVA linear polymers have a significant effect upon nanoprobe diffusion.

Probe Diffusion in Aqueous Poly(vinyl alcohol) Solutions Studied by Fluorescence Correlation Spectroscopy

2007 ◽  
Vol 8 (5) ◽  
pp. 1595-1600 ◽  
Author(s):  
Ariel Michelman-Ribeiro ◽  
Ferenc Horkay ◽  
Ralph Nossal ◽  
Hacène Boukari
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Vinyl Alcohol ◽  
Correlation Spectroscopy ◽  
Poly Vinyl Alcohol ◽  
Fluorescence Correlation

Fluorescence Correlation Spectroscopy Study of TAMRA Diffusion in Poly(vinyl-alcohol) and Ficoll70 Solutions

2005 ◽  
Vol 897 ◽  
Author(s):  
Ariel Michelman-Ribeiro ◽  
Ferenc Horkay ◽  
Ralph Nossal ◽  
Hacene Boukari
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Vinyl Alcohol ◽  
Scaling Law ◽  
Polymer Concentration ◽  
Correlation Spectroscopy ◽  
Poly Vinyl Alcohol ◽  
Translational Diffusion Coefficient ◽  
Fluorescence Correlation ◽  
Solvent Quality ◽  
Extract Information

AbstractWe compare fluorescence correlation spectroscopy (FCS) measurements of the fluorescent nanoparticle, TAMRA, diffusing in non-fluorescent -hence invisible- poly(vinyl-alcohol) (PVA) or Ficoll70 solutions as a function of the polymer concentration, c. We determine changes of the translational diffusion coefficient of TAMRA and fit the data with the universal scaling law (D ∼ exp[−α(c/c*)ν], to extract information about solvent quality. For PVA, we find ν = 0.74, suggesting that water in this case acts as a good solvent, whereas ν = 1.02 in Ficoll70 solutions, indicating theta-like behavior.

scholarly journals Nanofibre Electrospinning Poly(vinyl alcohol) and Cellulose Composite Mats Obtained by Use of a Cylindrical Electrode

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Anna Sutka ◽  
Silvija Kukle ◽  
Janis Gravitis ◽  
Rimvydas Milašius ◽  
Jolanta Malašauskienė
Keyword(s):  
Vinyl Alcohol ◽  
Steam Explosion ◽  
Combined Treatment ◽  
Poly Vinyl Alcohol ◽  
Cylindrical Electrode ◽  
Cellulose Nanofibres ◽  
Hemp Fibres ◽  
Pva Solutions ◽  
Atr Spectroscopy ◽  
Cellulose Composite

A study of nanofibre composites obtained by electrospinning from poly(vinyl alcohol) (PVA) solutions of steam exploded hemp fibres and shives is reported. A combined treatment of steam explosion (SE), ball milling, and high-intensity ultrasound (HIUS) is applied to prepare cellulose nanofibres (CNF) from hemp fibres (CNF-F) and shives (CNF-S). The reflectance Fourier transform infrared (FTIR ATR) spectroscopy is used to analyze the obtained PVA/CNF composite mats. Morphology of the PVA/CNF composites was studied by scanning electron microscopy (SEM).

Monitoring human parvovirus B19 virus-like particles and antibody complexes in solution by fluorescence correlation spectroscopy

2004 ◽  
Vol 385 (1) ◽  
pp. 87-93 ◽  
Author(s):  
J. Toivola ◽  
P. O. Michel ◽  
L. Gilbert ◽  
T. Lahtinen ◽  
V. Marjomäki ◽  
...  
Keyword(s):  
Diffusion Coefficient ◽  
Serum Sample ◽  
Acute Phase ◽  
Fluorescence Correlation Spectroscopy ◽  
Parvovirus B19 ◽  
Correlation Spectroscopy ◽  
Human Parvovirus B19 ◽  
Fluorescence Correlation ◽  
Average Diffusion Coefficient ◽  
Average Diffusion

AbstractFluorescence correlation spectroscopy (FCS) was used in monitoring human parvovirus B19 virus-like particle (VLP) antibody complexes from acute phase and pastimmunity serum samples. The Oregon Green 488-labeled VLPs gave an average diffusion coefficient of 1.7x10exp-7 cm(2)s(-1) with an apparent hydrodynamic radius of 14 nm. After incubation of the fluorescent VLPs with an acute phase serum sample, the mobility information obtained from the fluorescence intensity fluctuation by autocorrelation analysis showed an average diffusion coefficient of 1.5x10exp-8 cm(2)s(-1), corresponding to an average radius of 157 nm. In contrast, incubation of the fluorescent VLPs with a pastimmunity serum sample gave an average diffusion coefficient of 3.5x10exp-8 cm(2)s(-1) and a radius of 69 nm. A control serum devoid of B19 antibodies caused a change in the diffusion coefficient from 1.7x10exp-7 to 1.6x10exp-7 cm(2)s(-1), which is much smaller than that observed with acute phase or pastimmunity sera. Thus, VLP-antibody complexes with different diffusion coefficients could be identified for the acute phase and pastimmunity sera. FCS measurement of VLPimmune complexes could be useful in distinguishing between antibodies present in acute phase or past-immunity sera as well as in titration of the VLPs.

scholarly journals Molecular diffusion in ternary poly(vinyl alcohol) solutions

2021 ◽  
Author(s):  
Katarzyna Majerczak ◽  
Ophelie Squillace ◽  
Zhiwei Shi ◽  
Zhanping Zhang ◽  
Zhenyu J. Zhang
Keyword(s):  
Rhodamine B ◽  
Vinyl Alcohol ◽  
Molecular Diffusion ◽  
Chemical Species ◽  
Correlation Spectroscopy ◽  
Molecular Probe ◽  
Poly Vinyl Alcohol ◽  
Industrial Complex ◽  
Crowding Effect ◽  
Diffusion Characteristics

AbstractThe diffusion kinetics of a molecular probe—rhodamine B—in ternary aqueous solutions containing poly(vinyl alcohol), glycerol, and surfactants was investigated using fluorescence correlation spectroscopy and dynamic light scattering. We show that the diffusion characteristics of rhodamine B in such complex systems is determined by a synergistic effect of molecular crowding and intermolecular interactions between chemical species. The presence of glycerol has no noticeable impact on rhodamine B diffusion at low concentration, but significantly slows down the diffusion of rhodamine B above 3.9% (w/v) due to a dominating steric inhibition effect. Furthermore, introducing surfactants (cationic/nonionic/anionic) to the system results in a decreased diffusion coefficient of the molecular probe. In solutions containing nonionic surfactant, this can be explained by an increased crowding effect. For ternary poly(vinyl alcohol) solutions containing cationic or anionic surfactant, surfactant—polymer and surfactant—rhodamine B interactions alongside the crowding effect of the molecules slow down the overall diffusivity of rhodamine B. The results advance our insight of molecular migration in a broad range of industrial complex formulations that incorporate multiple compounds, and highlight the importance of selecting the appropriate additives and surfactants in formulated products.

scholarly journals A Comprehensive Review of Fluorescence Correlation Spectroscopy

2021 ◽  
Vol 9 ◽  
Author(s):  
Lan Yu ◽  
Yunze Lei ◽  
Ying Ma ◽  
Min Liu ◽  
Juanjuan Zheng ◽  
...  
Keyword(s):  
Diffusion Coefficient ◽  
Fluorescence Correlation Spectroscopy ◽  
Pair Correlation ◽  
Time Correlation ◽  
Correlation Spectroscopy ◽  
Local Concentration ◽  
Fluorescence Correlation ◽  
Pros And Cons

Fluorescence correlation spectroscopy (FCS) is a powerful technique for quantification of molecular dynamics, and it has been widely applied in diverse fields, e.g., biomedicine, biophysics, and chemistry. By time-correlation of the fluorescence fluctuations induced by molecules diffusing through a focused light, FCS can quantitatively evaluate the concentration, diffusion coefficient, and interaction of the molecules in vitro or in vivo. In this review, the basic principle and implementation of FCS are introduced. Then, the advances of FCS variants are reviewed, covering dual-color FCCS, multi-focus FCS, pair correlation function (pCF), scanning FCS, focus-reduced FCS, SPIM-FCS, and inverse-FCS. Besides, the applications of FCS are demonstrated with the measurement of local concentration, hydrodynamic radius, diffusion coefficient, and the interaction of different molecules. Lastly, a discussion is given by summarizing the pros and cons of different FCS techniques, as well as the outlooks and perspectives of FCS.

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