Fluorescence Correlation Spectroscopy Study of Probe Diffusion in Poly(vinyl alcohol) Solutions and Gels

2005 ◽  
Vol 227 (1) ◽  
pp. 221-230 ◽  
Author(s):  
Ariel Michelman-Ribeiro ◽  
Hacène Boukari ◽  
Ralph Nossal ◽  
Ferenc Horkay
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Vinyl Alcohol ◽  
Correlation Spectroscopy ◽  
Poly Vinyl Alcohol ◽  
Spectroscopy Study ◽  
Fluorescence Correlation

Probe Diffusion in Aqueous Poly(vinyl alcohol) Solutions Studied by Fluorescence Correlation Spectroscopy

2007 ◽  
Vol 8 (5) ◽  
pp. 1595-1600 ◽  
Author(s):  
Ariel Michelman-Ribeiro ◽  
Ferenc Horkay ◽  
Ralph Nossal ◽  
Hacène Boukari
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Vinyl Alcohol ◽  
Correlation Spectroscopy ◽  
Poly Vinyl Alcohol ◽  
Fluorescence Correlation

Fluorescence Correlation Spectroscopy Study of TAMRA Diffusion in Poly(vinyl-alcohol) and Ficoll70 Solutions

2005 ◽  
Vol 897 ◽  
Author(s):  
Ariel Michelman-Ribeiro ◽  
Ferenc Horkay ◽  
Ralph Nossal ◽  
Hacene Boukari
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Vinyl Alcohol ◽  
Scaling Law ◽  
Polymer Concentration ◽  
Correlation Spectroscopy ◽  
Poly Vinyl Alcohol ◽  
Translational Diffusion Coefficient ◽  
Fluorescence Correlation ◽  
Solvent Quality ◽  
Extract Information

AbstractWe compare fluorescence correlation spectroscopy (FCS) measurements of the fluorescent nanoparticle, TAMRA, diffusing in non-fluorescent -hence invisible- poly(vinyl-alcohol) (PVA) or Ficoll70 solutions as a function of the polymer concentration, c. We determine changes of the translational diffusion coefficient of TAMRA and fit the data with the universal scaling law (D ∼ exp[−α(c/c*)ν], to extract information about solvent quality. For PVA, we find ν = 0.74, suggesting that water in this case acts as a good solvent, whereas ν = 1.02 in Ficoll70 solutions, indicating theta-like behavior.

Nanoprobe Diffusion in Poly(Vinyl-alcohol) Gels and Solutions: Effects of pH and Dehydration

2014 ◽  
Vol 1622 ◽  
pp. 135-145
Author(s):  
Hacène Boukari ◽  
Candida Silva ◽  
Ralph Nossal ◽  
Ferenc Horkay
Keyword(s):  
Diffusion Coefficient ◽  
Vinyl Alcohol ◽  
Correlation Spectroscopy ◽  
Poly Vinyl Alcohol ◽  
Linear Polymers ◽  
Fluorescence Correlation ◽  
Slowing Down ◽  
Effects Of Ph ◽  
Physical Interactions ◽  
Pva Solutions

ABSTRACTWe report fluorescence correlation spectroscopy (FCS) measurements of the translational diffusion of two fluorescent nanoprobes, rhodamine (R6G) and carboxytetramethylrhodamine (TAMRA), embedded in poly(vinyl alcohol) (PVA) solutions and gels. The diffusion coefficient was measured as a function of the PVA concentration and pH. Furthermore, we designed and built an optical chamber to determine the diffusion coefficient of the nanoprobes within the PVA solutions and gels subjected to controlled dehydration. We find that 1) lowering pH causes an apparent slowing down of the diffusion of the nanoprobes, 2) increase of PVA concentration and crosslink density also induce slowing down of both nanoprobes, and 3) dehydration induces systematic decrease of the diffusion of TAMRA in both solutions and gels. Taken together, these results demonstrate that transient physical interactions between the nanoprobes and the PVA linear polymers have a significant effect upon nanoprobe diffusion.

scholarly journals The oligomeric state and stability of the mannitol transporter, EnzymeIImtl, fromEscherichia coli: A fluorescence correlation spectroscopy study

2006 ◽  
Vol 15 (8) ◽  
pp. 1977-1986 ◽  
Author(s):  
Gertjan Veldhuis ◽  
Mark Hink ◽  
Victor Krasnikov ◽  
Geert van den Bogaart ◽  
Jeroen Hoeboer ◽  
...  
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Spectroscopy Study ◽  
Oligomeric State ◽  
Fluorescence Correlation ◽  
Fromescherichia Coli

scholarly journals Fluorescence correlation spectroscopy study of protein transport and dynamic interactions with clustered-charge peptide adsorbents

2012 ◽  
Vol 25 (8) ◽  
pp. 435-442 ◽  
Author(s):  
Charlisa R. Daniels ◽  
Lydia Kisley ◽  
Hannah Kim ◽  
Wen-Hsiang Chen ◽  
Mohan-Vivekanandan Poongavanam ◽  
...  
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Protein Transport ◽  
Correlation Spectroscopy ◽  
Spectroscopy Study ◽  
Dynamic Interactions ◽  
Fluorescence Correlation
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