Modular Peptide-Based Hybrid Nanoprobes for Bio-Imaging and Bio-Sensing

2014 ◽  
Vol 1621 ◽  
pp. 155-161 ◽  
Author(s):  
Banu Taktak Karaca ◽  
James Meyer ◽  
Sarah VanOosten ◽  
Mark Richter ◽  
Candan Tamerler
Keyword(s):  
Self Assembly ◽  
Copper Ions ◽  
Copper Ion ◽  
Gold Surface ◽  
Protein Arrays ◽  
Recognition Element ◽  
Ion Sensing ◽  
Wide Range ◽  
Biological Recognition ◽  
Assembly Technique

ABSTRACTThe self-organization of functional proteins directly onto solid materials is attractive to a wide range of biomaterials and systems that need to accommodate a biological recognition element. In such systems, inorganic binding peptides may be an essential component due to their high affinity and selective binding features onto different types of solid surfaces. This study demonstrates a peptide-enabled self-assembly technique for designing well-defined protein arrays over a metal surface. To illustrate this concept, we designed a fusion protein that simultaneously displays a red fluorescence protein (DsRed-monomer), which is highly selective for copper ions, and a gold binding peptide AuBP. The peptide tag, AuBP, self-directs the organization of DsRed-monomer protein onto a gold surface and forms arrays built upon an efficient control of the organic/inorganic interface at the molecular level. The peptide-assisted design offers a modular approach for fabrication of fluorescent-based protein arrays with copper ion sensing ability.

scholarly journals X-Ray Crystallographic Analysis, EPR Studies, and Computational Calculations of a Cu(II) Tetramic Acid Complex

2017 ◽  
Vol 2017 ◽  
pp. 1-10
Author(s):  
Dimitrios Matiadis ◽  
Dimitrios Tsironis ◽  
Valentina Stefanou ◽  
Olga Igglessi–Markopoulou ◽  
Vickie McKee ◽  
...  
Keyword(s):  
Self Assembly ◽  
Copper Ions ◽  
Copper Ion ◽  
Spectroscopic Properties ◽  
Crystallographic Analysis ◽  
Computational Techniques ◽  
Tetramic Acid ◽  
Acid Complex ◽  
X Ray ◽  
Computational Calculations

In this work we present a structural and spectroscopic analysis of a copper(II) N-acetyl-5-arylidene tetramic acid by using both experimental and computational techniques. The crystal structure of the Cu(II) complex was determined by single crystal X-ray diffraction and shows that the copper ion lies on a centre of symmetry, with each ligand ion coordinated to two copper ions, forming a 2D sheet. Moreover, the EPR spectroscopic properties of the Cu(II) tetramic acid complex were also explored and discussed. Finally, a computational approach was performed in order to obtain a detailed and precise insight of product structures and properties. It is hoped that this study can enrich the field of functional supramolecular systems, giving place to the formation of coordination-driven self-assembly architectures.

HYBRID SEMICONDUCTING QUANTUM DOTS–METALLIC NANOPARTICLES ARRAYS FOR POSSIBLE NANOPHOTONIC DEVICES

2011 ◽  
Vol 10 (04n05) ◽  
pp. 1113-1118
Author(s):  
M. HARIDAS ◽  
J. K. BASU
Keyword(s):  
Quantum Dots ◽  
Self Assembly ◽  
Metallic Nanoparticles ◽  
Quantum Information Processing ◽  
Imaging Techniques ◽  
Large Area ◽  
Nanophotonic Devices ◽  
Wide Range ◽  
Potential Applications ◽  
Assembly Technique

Arrays of quantum dots and hybrid arrays of semiconducting quantum dots and metallic nanoparticles have wide range of potential applications from nanophotonics to quantum information processing. Creating such arrays with well-defined morphology and order over a large area is a challenge. We present a reliable method for constructing such arrays using simple self assembly technique. The reliability of the method is verified using AFM. The emission properties of such system are studied using high resolution imaging techniques and we have given the possible explanation for the observed phenomena.

The Relationship between Growth Speed and Ambient Humidity in Convective Self-assembly

2010 ◽  
Vol 1273 ◽  
Author(s):  
Hans D. Robinson ◽  
Kai Chen ◽  
Stefan V. Stoianov
Keyword(s):  
Growth Rate ◽  
Self Assembly ◽  
Standard Technique ◽  
Crystal Growth Rate ◽  
Growth Speed ◽  
Linear Dependency ◽  
Ambient Relative Humidity ◽  
Wide Range ◽  
Assembly Technique ◽  
The Relationship

AbstractWe present a variation of a standard convective self-assembly technique, where the drying meniscus is restricted by a straight-edge located approximately 100 μm above the substrate adjacent to the drying zone. We find this technique to yield films at roughly twice the growth rate compared to the standard technique. We attribute this to differing local evaporation rates in the two cases. We also investigate how the crystal growth rate depends on ambient relative humidity and find a clear linear dependency, which we attribute to the length of the drying zone being constant over a wide range of humidities.

scholarly journals A Disposable Copper (II) Ion Biosensor Based on Self-Assembly of L-Cysteine on Gold Nanoparticle-Modified Screen-Printed Carbon Electrode

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Wong Pooi See ◽  
Sheila Nathan ◽  
Lee Yook Heng
Keyword(s):  
Gold Nanoparticle ◽  
Self Assembly ◽  
Copper Ions ◽  
Effective Means ◽  
Copper Ion ◽  
Differential Pulse ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Preconcentration Time ◽  
Screen Printed

A disposable copper (II) ion biosensor based on self-assembly of L-cysteine on gold nanoparticle-modified screen-printed carbon electrode was fabricated. The electrode was modified by attaching gold nanoparticles onto the surface of screen-printed carbon electrode through seed mediated growth method followed by self-assembly of L-cysteine. As demonstrated by differential pulse voltammetry, the sensor exhibited high sensitivity to copper (II) ion down to ppb (parts per billion) levels. Optimization of various experimental parameters such as pH, buffer concentration, and preconcentration time, which influenced the performance of the biosensor, was investigated. The sensor demonstrated a wide linear response range from 10 to 0.005 ppm(r=0.9870), with a lower detection limit of 8 ppb using 10 min of preconcentration time. The sensor based on screen-printed electrode provides a cost-effective means of application of copper ion sensor for the detection of ppb level of copper ions in water.

scholarly journals A Rare Natural Benzo[k,l]xanthene as a Turn-Off Fluorescent Sensor for Cu2+ Ion

2020 ◽  
Vol 21 (18) ◽  
pp. 6933
Author(s):  
Giuseppe Floresta ◽  
Nunzio Cardullo ◽  
Carmela Spatafora ◽  
Antonio Rescifina ◽  
Corrado Tringali
Keyword(s):  
Fluorescent Probe ◽  
Fluorescence Intensity ◽  
Limit Of Detection ◽  
Copper Ions ◽  
Fluorescent Sensor ◽  
Copper Ion ◽  
Living Cells ◽  
A Value ◽  
Wide Range ◽  
Catechol Group

Rapid and efficient analyses of copper ions are crucial to providing key information for Cu2+ in living cells because of their biological importance. In this study, we reported one new turn-off fluorescent sensor for Cu2+ with a benzo[k,l]xanthene core, which served as an efficient cation sensor for copper ion over a wide range of other cations (Na+, K+, Ag+, Hg2+, Cd2+, Co2+, Ni2+, Zn2+, Mg2+, and Fe3+) owing to the catechol group in the aromatic core. The sensor showed selectivity for Cu2+ over other ions; the logKβ for Cu2+ binding to compound 1 had a value of 13.265. In the presence of Cu2+, sensor 1 provided significant fluorescence decrement; Co2+, and Ni2+ caused a fluorescence decrement when employed at a higher concentration than Cu2+, while Na+, K+, Hg2+, Cd2+, Zn2+, and Mg2+ metal ions produced only minor changes in fluorescence intensity. Fluorescence experiments demonstrate that compound 1 may have an application as a fluorescent probe for detecting Cu2+ with a limit of detection of 0.574 µM.

scholarly journals A Binary Supramolecular Assembly with Intense Fluorescence Emission, High pH Stability, and Cation Selectivity: Supramolecular Assembly-Induced Emission Materials

Research ◽  
2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Xu Wang ◽  
Xin-Yue Lou ◽  
Xiao-Yu Jin ◽  
Feng Liang ◽  
Ying-Wei Yang
Keyword(s):  
Self Assembly ◽  
Inclusion Complexation ◽  
Fluorescence Emission ◽  
Blue Emission ◽  
Supramolecular Assembly ◽  
Supramolecular System ◽  
Cation Selectivity ◽  
Ion Sensing ◽  
Wide Range ◽  
Ammonium Binding

We construct a fluorescent supramolecular system (TPE-Q4⊂ DSP5) of excellent tolerance to a wide range of pH by the facile self-assembly of a new pillar[5]arene bearing disulfonated arms (DSP5) with an AIE-active tetraphenylethene-based tetratopic guest bearing four quaternary ammonium binding sites (TPE-Q4), which exhibits strong blue emission even in dilute aqueous solutions along with much higher quantum yield and longer fluorescence lifetime than TPE-Q4 itself. This appreciable property can be attributed to the supramolecular assembly-induced emission (SAIE) mechanism endowed by the host-guest inclusion complexation based on synthetic macrocycles. Remarkably, the enhanced fluorescence of the supramolecular assembly is quenched efficiently and exclusively by ferric ions in water with a high Stern–Volmer formula constant of 1.3 × 105  mol-1, demonstrating the excellent cation selectivity and visualized responsiveness in ion sensing and detection.

Factors Affecting Molecular Self-Assembly and Its Mechanism

2012 ◽  
Vol 9 (1) ◽  
pp. 43 ◽  
Author(s):  
Hueyling Tan
Keyword(s):  
Self Assembly ◽  
Building Blocks ◽  
Molecular Engineering ◽  
Molecular Structures ◽  
Polymer Science ◽  
New Approach ◽  
Factors Affecting ◽  
Dna Structures ◽  
Self Assembling ◽  
Wide Range

Molecular self-assembly is ubiquitous in nature and has emerged as a new approach to produce new materials in chemistry, engineering, nanotechnology, polymer science and materials. Molecular self-assembly has been attracting increasing interest from the scientific community in recent years due to its importance in understanding biology and a variety of diseases at the molecular level. In the last few years, considerable advances have been made in the use ofpeptides as building blocks to produce biological materials for wide range of applications, including fabricating novel supra-molecular structures and scaffolding for tissue repair. The study ofbiological self-assembly systems represents a significant advancement in molecular engineering and is a rapidly growing scientific and engineering field that crosses the boundaries ofexisting disciplines. Many self-assembling systems are rangefrom bi- andtri-block copolymers to DNA structures as well as simple and complex proteins andpeptides. The ultimate goal is to harness molecular self-assembly such that design andcontrol ofbottom-up processes is achieved thereby enabling exploitation of structures developed at the meso- and macro-scopic scale for the purposes oflife and non-life science applications. Such aspirations can be achievedthrough understanding thefundamental principles behind the selforganisation and self-synthesis processes exhibited by biological systems.

scholarly journals Polymer cyclization for the emergence of hierarchical nanostructures

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Chaojian Chen ◽  
Manjesh Kumar Singh ◽  
Katrin Wunderlich ◽  
Sean Harvey ◽  
Colette J. Whitfield ◽  
...  
Keyword(s):  
Self Assembly ◽  
Three Dimensional ◽  
Hierarchical Structures ◽  
Structural Similarity ◽  
Vital Role ◽  
Nanomaterial Synthesis ◽  
Wide Range ◽  
Linear Poly ◽  
Polymer Folding ◽  
Dynamics Simulations

AbstractThe creation of synthetic polymer nanoobjects with well-defined hierarchical structures is important for a wide range of applications such as nanomaterial synthesis, catalysis, and therapeutics. Inspired by the programmability and precise three-dimensional architectures of biomolecules, here we demonstrate the strategy of fabricating controlled hierarchical structures through self-assembly of folded synthetic polymers. Linear poly(2-hydroxyethyl methacrylate) of different lengths are folded into cyclic polymers and their self-assembly into hierarchical structures is elucidated by various experimental techniques and molecular dynamics simulations. Based on their structural similarity, macrocyclic brush polymers with amphiphilic block side chains are synthesized, which can self-assemble into wormlike and higher-ordered structures. Our work points out the vital role of polymer folding in macromolecular self-assembly and establishes a versatile approach for constructing biomimetic hierarchical assemblies.

scholarly journals An Investigation on Design and Characterization of a Highly Selective LED Optical Sensor for Copper Ions in Aqueous Solutions

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1099
Author(s):  
Sheng-Chun Hung ◽  
Chih-Cheng Lu ◽  
Yu-Ting Wu
Keyword(s):  
Aqueous Solutions ◽  
Metal Ions ◽  
Copper Ions ◽  
Incident Light ◽  
Copper Ion ◽  
Intensity Change ◽  
Optical Absorbance ◽  
Sensing System ◽  
Optic Sensor ◽  
Cu Ions

The optical characteristics of copper ion detection, such as the photometric absorbance of specific wavelengths, exhibit significant intensity change upon incident light into the aqueous solutions with different concentrations of metal ions due to the electron transition in the orbit. In this study, we developed a low-cost, small-size and fast-response photoelectric sensing prototype as an optic sensor for copper (Cu) ions detection by utilizing the principle of optical absorption. We quantified the change of optical absorbance from infra-red (IR) light emitting diodes (LEDs) upon different concentrations of copper ions and the transmitted optical signals were transferred to the corresponding output voltage through a phototransistor and circuit integrated in the photoelectric sensing system. The optic sensor for copper (Cu) ions demonstrated not only excellent specificity with other metal ions such as cadmium (Cd), nickel (Ni), iron (Fe) and chloride (Cl) ions in the same aqueous solution but also satisfactory linearity and reproducibility. The sensitivity of the preliminary sensing system for copper ions was 29 mV/ppm from 0 to 1000 ppm. In addition, significant ion-selective characteristics and anti-interference capability were also observed in the experiments by the proposed approach.

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