Marine Adhesive Containing Nanocomposite Hydrogel with Enhanced Materials and Bioadhesive Properties

2013 ◽  
Vol 1569 ◽  
pp. 33-38 ◽  
Author(s):  
Yuan Liu ◽  
Hao Zhan ◽  
Sarah Skelton ◽  
Bruce P. Lee
Keyword(s):  
Network Formation ◽  
Gelation Time ◽  
Work Of Adhesion ◽  
Tissue Adhesive ◽  
Nanocomposite Hydrogel ◽  
Adhesive Properties ◽  
Covalent Bonds ◽  
Materials Properties ◽  
Lap Shear ◽  
Mussel Adhesive

ABSTRACT4-arm poly(ethylene glycol) end-capped with mimics of adhesive moiety found in mussel adhesive protein, dopamine, was combined with a biocompatible nano-silicate, Laponite, in creating a nanocomposite hydrogel with improved materials and adhesive properties. Dopamine’s ability to form both irreversible covalent (cohesive and interfacial) and reversible physical (with Laponite) crosslinks was exploited in creating an injectable tissue adhesive. Incorporation of Laponite did not interfere with the curing of the adhesive. In some instances, increasing Laponite content reduced gelation time as dopamine-Laponite bond reduced the required number of covalent bonds needed for network formation. Incorporation of Laponite also increased compressive materials properties (e.g., max strength, energy to failure, etc.) of the nanocomposite without compromising its compliance as strain at failure was also increased. From lap shear adhesion test using wetted pericardium as the substrate, incorporating Laponite increased work of adhesion by 5 fold over that of control. Strong, physical bonds formed between dopamine and Laponite increased bulk materials properties, which contributed to the enhanced adhesive properties.

scholarly journals Formulation and Functional Properties of Whey Protein-Based Tissue Adhesive Using Totarol as an Antimicrobial Agent

Processes ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 496
Author(s):  
Yifan Hou ◽  
Xiaonan Zhang ◽  
Cuina Wang ◽  
Mingruo Guo
Keyword(s):  
Antimicrobial Agent ◽  
Whey Protein ◽  
Bonding Strength ◽  
Crosslinking Agent ◽  
Tissue Adhesive ◽  
Plate Count ◽  
Tissue Adhesives ◽  
Adhesive Properties ◽  
Lap Shear ◽  
Shear Bonding Strength

Tissue adhesives have been widely used in surgical procedures. Compared to traditional surgical sutures, tissue adhesives provide fast bonding experiences and full closure of wounds. However, current tissue adhesives are mostly fossil-based synthetic products. Therefore, it is of great significance to explore the use of natural materials in tissue adhesives. Whey is a low-end byproduct of cheese manufacturing. Whey protein, a group of small globular proteins, can exhibit adhesive properties if their structures are modified by physical or chemical means. The objectives of this study were to investigate the functional and structural properties of whey protein-based tissue adhesive, along with the antibacterial effect of totarol, a natural antimicrobial agent. Whey protein isolate (WPI) solutions (25%–33% protein) were mixed with different levels (0.1%–0.3% w/w) of totarol. The mixtures were analyzed for total plate count and yeast and mold count. The lap-shear bonding strength was tested after the WPI-totarol solutions were mixed with a crosslinking agent, glutaraldehyde (GTA). The lap-shear bonding strength of the tissue adhesive was about 20 kPa, which is comparable to that of a commercial BioGlue®. The microstructures of the mixtures were analyzed by scanning electron microscopy (SEM).

Preparation and Characterization of Drug-Loaded Thermosensitive Hydrogels

2013 ◽  
Author(s):  
L. Saeednia ◽  
A. Usta ◽  
R. Asmatulu
Keyword(s):  
Drug Delivery ◽  
Controlled Release ◽  
Body Temperature ◽  
Network Formation ◽  
Gelation Time ◽  
The Body ◽  
Antibacterial Drug ◽  
Natural Polymers ◽  
3D Network ◽  
Thermosensitive Hydrogels

Hydrogels are the promising classes of polymeric drug delivery systems with the controlled release rates. Among them, injectable thermosensitive hydrogels with transition temperature around the body temperature have been wildly considered. Chitosan is one of the most abundant natural polymers, and its biocompatibility and biodegradability makes it a favorable thermosensitive hydrogel that has been attracted much attention in biomedical field worldwide. In this work, a thermosensitive and injectable hydrogel was prepared using chitosan and β-glycerophosphate (β-GP) incorporated with an antibacterial drug (gentamycin). This drug loaded hydrogel is liquid at room temperature, and becomes more solidified gel when heated to the body temperature. Adding β-GP into chitosan and drug molecules and heating the overall solution makes the whole homogenous liquid into gel through a 3D network formation. The gelation time was found to be a function of temperature and concentration of β-GP. This thermosensitive chitosan based hydrogel system was characterized using FTIR and visual observation to determine the chemical structure and morphology. The results confirmed that chitosan/(β-GP) hydrogels could be a promising controlled-release drug delivery system for many deadly diseases.

First Aldol Cross-Linked Hyaluronic Acid Hydrogel: Fast and Hydrolytically Stable Hydrogel with Tissue Adhesive Properties

2019 ◽  
Vol 11 (41) ◽  
pp. 38232-38239 ◽  
Author(s):  
Daniel Bermejo-Velasco ◽  
Sandeep Kadekar ◽  
Marcus Vinicius Tavares da Costa ◽  
Oommen P. Oommen ◽  
Kristofer Gamstedt ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Tissue Adhesive ◽  
Adhesive Properties ◽  
Hyaluronic Acid Hydrogel

Rapid in situ cross-linking of hydrogel adhesives based on thiol-grafted bio-inspired catechol-conjugated chitosan

2017 ◽  
Vol 32 (5) ◽  
pp. 612-621 ◽  
Author(s):  
Zhiwen Zeng ◽  
Xiumei Mo
Keyword(s):  
Soft Tissues ◽  
Sodium Periodate ◽  
Oxidation Mechanism ◽  
Thiol Group ◽  
In Vitro Cytotoxicity ◽  
Tissue Adhesive ◽  
Molar Ratio ◽  
Adhesive Properties

In this paper, a novel chitosan derivative, thiol-grafting bio-inspired catechol-conjugated chitosan was synthesized. The chemical structure of the synthesized catechol-conjugated chitosan was verified by 1H NMR, and its contents of thiol group and catechol group were determined by UV-vis spectrum. Four percent of catechol-conjugated chitosan aqueous solution could form hydrogels rapidly in situ in 1 min or less with the addition of sodium periodate. Rheological studies showed that the mechanical properties depend on the concentrations of catechol-conjugated chitosan and the molar ratio of sodium periodate to catechol groups. Additionally, the adhesive properties of the resulting adhesives were evaluated, and the adhesion strength of obtained adhesives was as high as 50 kPa because of the complex and multiple interactions, especially the anti-oxidation mechanism of thiol group. The in vitro cytotoxicity assays demonstrated an excellent biocompatibility of the catechol-conjugated chitosan hydrogels. Benefiting from the in situ fast cured, desired mechanical strength, biocompatibility and relatively high adhesion performance, these properties suggested that catechol-conjugated chitosan hydrogel adhesives have potential applications as tissue adhesive for soft tissues.

Physical and Adhesive Properties of Cyanoacrylate-Based β-TCP Composites

2007 ◽  
Vol 330-332 ◽  
pp. 419-422 ◽  
Author(s):  
G.S. Lee ◽  
Sang Bae Lee ◽  
Doug Youn Lee ◽  
Kyeong Jun Park ◽  
S.O. Kim ◽  
...  
Keyword(s):  
Compressive Strength ◽  
Citric Acid ◽  
Bond Strength ◽  
Shear Bond Strength ◽  
Setting Time ◽  
Tissue Adhesive ◽  
Adhesive Properties ◽  
Composite Systems ◽  
Filling Materials

Histoacryl® (N-butyl-2-cyanoacrylate) has been widely utilized as a tissue adhesive. The aim of this study was to evaluate the physical and adhesive properties of newly developed cyanoacrylate-based β-TCP composite systems. The β-TCP powder was modified on the surface with citric acid to make this material mixed with cyanoacrylate easily. The setting time of acidtreated β-TCP/ Histoacryl® systems was dramatically prolonged and the polymerization heat was significantly decreased compared to that of untreated β-TCP/Histoacryl® system. The shear bond strength of cyanoacrylate-based β-TCP composites decreased with addition of acid-treated β-TCP filler. The compressive strength of β-TCP/Histoacryl® composites increased strongly with increasing the amount of acid-treated β-TCP filler. The cytotoxicity of the β-TCP/Histoacryl® composites decreased with the increasing of the amount of added β-TCP. These results indicated that our novel β-TCP/Histoacryl® composites had the great potential to serve as adhesives or filling materials in the dental field.

scholarly journals Expression of Functional Recombinant Mussel Adhesive Protein Mgfp-5 in Escherichia coli

2004 ◽  
Vol 70 (6) ◽  
pp. 3352-3359 ◽  
Author(s):  
Dong Soo Hwang ◽  
Hyo Jin Yoo ◽  
Jong Hyub Jun ◽  
Won Kyu Moon ◽  
Hyung Joon Cha
Keyword(s):  
Escherichia Coli ◽  
Adhesion Force ◽  
Soluble Form ◽  
Material Surface ◽  
Adhesive Properties ◽  
Affinity Ligand ◽  
Mussel Adhesive Proteins ◽  
Mussel Adhesive ◽  
Aqueous Conditions ◽  
Adhesive Proteins

ABSTRACT Mussel adhesive proteins have been suggested as a basis for environmentally friendly adhesives for use in aqueous conditions and in medicine. However, attempts to produce functional and economical recombinant mussel adhesive proteins (mainly foot protein type 1) in several systems have failed. Here, the cDNA coding for Mytilus galloprovincialis foot protein type 5 (Mgfp-5) was isolated for the first time. Using this cDNA, we produced a recombinant Mgfp-5 fused with a hexahistidine affinity ligand, which was expressed in a soluble form in Escherichia coli and was highly purified using affinity chromatography. The adhesive properties of purified recombinant Mgfp-5 were compared with the commercial extracted mussel adhesive Cell-Tak by investigating adhesion force using atomic force microscopy, material surface coating, and quartz crystal microbalance. Even though further macroscale assays are needed, these microscale assays showed that recombinant Mgfp-5 has significant adhesive ability and may be useful as a bioadhesive in medical or underwater environments.

Effect of Process Variable on the Gelation Time of Sulfonated Polyacrylamide Nanocomposite Hydrogel

2014 ◽  
Vol 35 (5) ◽  
pp. 663-671 ◽  
Author(s):  
Mahsa Baghban Salehi ◽  
Mohsen Vafaie Sefti ◽  
Ebrahim Vasheghani-Farahani ◽  
Asefe Mousavi Moghadam
Keyword(s):  
Gelation Time ◽  
Process Variable ◽  
Nanocomposite Hydrogel ◽  
Sulfonated Polyacrylamide

Study on Bone Cell Adaptability of α-TCP/HAp Functionally Graded Porous Beads for Biomaterials Application

2010 ◽  
Vol 76 ◽  
pp. 143-146
Author(s):  
S. Ohtake ◽  
T. Asaoka ◽  
K. Furukawa ◽  
T. Ushida ◽  
T. Tateishi
Keyword(s):  
Bone Cells ◽  
High Strength ◽  
Bone Cell ◽  
Functionally Graded ◽  
The Body ◽  
Tissue Adhesive ◽  
Adhesive Properties ◽  
Living Body ◽  
Porous Beads

Porous beads of bioactive ceramics such as HAp, TCP are considered to be promising as excellent scaffolds for cultivating bone cells. To realize this type of beads which maintains the function of scaffold with sufficient strength up to growth of new bone, and is expected to absorbed completely after the growth, a-TCP/ HAp functionally graded porous beads were fabricated. HAp is bioactive material which has both high strength and better tissue-adhesive properties, but that is not readily absorbed by the human body. On the contrary, a-TCP is highly bioabsorbable; it is quickly absorbed by the body, and, therefore, disappears before bone is completely replaced. Fabricated new beads are composed of a-TCP at the center and HAp at the surface, to control the solubility in living body. Bone cell adaptability of these beads were confirmed in vitro.

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