Preparation of Nanoparticle-containing Aligned Collagen Fibers for Dense Connective Tissue Repair and Regeneration

2012 ◽  
Vol 1417 ◽  
Author(s):  
Xingguo Cheng ◽  
Sapna A. Desai
Keyword(s):  
Growth Factor ◽  
Tissue Repair ◽  
Collagen Fiber ◽  
Human Platelet ◽  
Electrochemical Process ◽  
Collagen Fibers ◽  
Factor H ◽  
Composite Fibers ◽  
Tissue Repair And Regeneration ◽  
Connective Tissue Repair

ABSTRACTNanoparticles (NPs) loaded with human platelet-derived growth factor (h-PDGF) were prepared and characterized. These NPs were co-assembled with collagen to form highly aligned NP-collagen composite fibers by using an electrochemical process. PDGF can be released gradually from either NPs or aligned NP-collagen fibers. This investigation demonstrated a novel way to fabricate highly aligned composite fibers, which can locally release a growth factor in a controlled and gradual manner, potentially avoiding the fast clearance of the growth factor from the implantation site. Thus, the aligned NP-collagen fiber is a novel and promising implantation material for tendon/ligament repair and regeneration.

scholarly journals Design of epidermal growth factor immobilization on 3D biocompatible scaffolds to promote tissue repair and regeneration

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Teodora Bavaro ◽  
Sara Tengattini ◽  
Refaya Rezwan ◽  
Enrica Chiesa ◽  
Caterina Temporini ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Tissue Repair ◽  
Peptide Mapping ◽  
Rnase A ◽  
Fibroblast Cells ◽  
Native State ◽  
Tissue Repair And Regeneration ◽  
Epidermal Growth ◽  
Cell Proliferative Activity

AbstractExogenous application of human epidermal growth factor (hEGF) stimulates epidermal wound healing. The aim of this study was to develop bioconjugates based on hEGF mimicking the protein in its native state and thus suitable for tissue engineering applications, in particular for treating skin-related disorders as burns. Ribonuclease A (RNase A) was used to investigate a number of different activated-agarose carriers: cyanogen bromide (CNBr)-activated-agarose and glyoxyl-agarose showed to preserve the appropriate orientation of the protein for receptor binding. EGF was immobilized on these carriers and immobilization yield was evaluated (100% and 12%, respectively). A peptide mapping of unbound protein regions was carried out by LC–MS to take evidence of the residues involved in the immobilization and, consequently, the flexibility and surface accessibility of immobilized EGF. To assess cell proliferative activities, 10, 25, 50, and 100 ng/mL of each immobilized EGF sample were seeded on fibroblast cells and incubated for 24, 48 and 72 h. The immobilized growth factor showed significantly high cell proliferative activity at 50 and 100 ng/mL compared to control and soluble EGF. Although both of the immobilized samples show dose-dependency when seeded with high number of fibroblast cells, CNBr-agarose-EGF showed a significantly high activity at 100 ng/mL and 72 h incubation, compared to glyoxyl-agarose-EGF.

scholarly journals Injectable Biodegradable Polyurethane Scaffolds with Release of Platelet-derived Growth Factor for Tissue Repair and Regeneration

2008 ◽  
Vol 25 (10) ◽  
pp. 2387-2399 ◽  
Author(s):  
Andrea E. Hafeman ◽  
Bing Li ◽  
Toshitaka Yoshii ◽  
Katarzyna Zienkiewicz ◽  
Jeffrey M. Davidson ◽  
...  
Keyword(s):  
Growth Factor ◽  
Tissue Repair ◽  
Platelet Derived Growth Factor ◽  
Tissue Repair And Regeneration ◽  
Biodegradable Polyurethane

scholarly journals Heparin-Binding Epidermal Growth Factor–Like Growth Factor as a Critical Mediator of Tissue Repair and Regeneration

2018 ◽  
Vol 188 (11) ◽  
pp. 2446-2456 ◽  
Author(s):  
Duy T. Dao ◽  
Lorenzo Anez-Bustillos ◽  
Rosalyn M. Adam ◽  
Mark Puder ◽  
Diane R. Bielenberg
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Tissue Repair ◽  
Heparin Binding ◽  
Tissue Repair And Regeneration ◽  
Epidermal Growth
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