scholarly journals Heparin-Binding Epidermal Growth Factor–Like Growth Factor as a Critical Mediator of Tissue Repair and Regeneration

2018 ◽  
Vol 188 (11) ◽  
pp. 2446-2456 ◽  
Author(s):  
Duy T. Dao ◽  
Lorenzo Anez-Bustillos ◽  
Rosalyn M. Adam ◽  
Mark Puder ◽  
Diane R. Bielenberg
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Tissue Repair ◽  
Heparin Binding ◽  
Tissue Repair And Regeneration ◽  
Epidermal Growth

scholarly journals Design of epidermal growth factor immobilization on 3D biocompatible scaffolds to promote tissue repair and regeneration

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Teodora Bavaro ◽  
Sara Tengattini ◽  
Refaya Rezwan ◽  
Enrica Chiesa ◽  
Caterina Temporini ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Tissue Repair ◽  
Peptide Mapping ◽  
Rnase A ◽  
Fibroblast Cells ◽  
Native State ◽  
Tissue Repair And Regeneration ◽  
Epidermal Growth ◽  
Cell Proliferative Activity

AbstractExogenous application of human epidermal growth factor (hEGF) stimulates epidermal wound healing. The aim of this study was to develop bioconjugates based on hEGF mimicking the protein in its native state and thus suitable for tissue engineering applications, in particular for treating skin-related disorders as burns. Ribonuclease A (RNase A) was used to investigate a number of different activated-agarose carriers: cyanogen bromide (CNBr)-activated-agarose and glyoxyl-agarose showed to preserve the appropriate orientation of the protein for receptor binding. EGF was immobilized on these carriers and immobilization yield was evaluated (100% and 12%, respectively). A peptide mapping of unbound protein regions was carried out by LC–MS to take evidence of the residues involved in the immobilization and, consequently, the flexibility and surface accessibility of immobilized EGF. To assess cell proliferative activities, 10, 25, 50, and 100 ng/mL of each immobilized EGF sample were seeded on fibroblast cells and incubated for 24, 48 and 72 h. The immobilized growth factor showed significantly high cell proliferative activity at 50 and 100 ng/mL compared to control and soluble EGF. Although both of the immobilized samples show dose-dependency when seeded with high number of fibroblast cells, CNBr-agarose-EGF showed a significantly high activity at 100 ng/mL and 72 h incubation, compared to glyoxyl-agarose-EGF.

scholarly journals Heparin-binding epidermal growth factor-like growth factor is an autocrine growth factor for human keratinocytes.

1994 ◽  
Vol 269 (31) ◽  
pp. 20060-20066 ◽  
Author(s):  
K. Hashimoto ◽  
S. Higashiyama ◽  
H. Asada ◽  
E. Hashimura ◽  
T. Kobayashi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Human Keratinocytes ◽  
Heparin Binding ◽  
Autocrine Growth ◽  
Autocrine Growth Factor ◽  
Epidermal Growth

scholarly journals Differential Pathways of Angiotensin II-Induced Extracellularly Regulated Kinase 1/2 Phosphorylation in Specific Cell Types: Role of Heparin-Binding Epidermal Growth Factor

2004 ◽  
Vol 18 (8) ◽  
pp. 2035-2048 ◽  
Author(s):  
Bukhtiar H. Shah ◽  
Akin Yesilkaya ◽  
J. Alberto Olivares-Reyes ◽  
Hung-Dar Chen ◽  
László Hunyady ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Types ◽  
Specific Cell ◽  
Heparin Binding ◽  
Epidermal Growth

scholarly journals Membrane-anchored Heparin-binding Epidermal Growth Factor-like Growth Factor Acts as a Tumor Survival Factor in a Hepatoma Cell Line

1997 ◽  
Vol 272 (22) ◽  
pp. 14349-14355 ◽  
Author(s):  
Eiji Miyoshi ◽  
Shigeki Higashiyama ◽  
Takatoshi Nakagawa ◽  
Norio Hayashi ◽  
Naoyuki Taniguchi
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Line ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Heparin Binding ◽  
Survival Factor ◽  
Tumor Survival ◽  
Epidermal Growth
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