Prevention of Candida biofilm formation over polystyrene by plasma polymerization technique

2020 ◽  
Vol 10 (4) ◽  
pp. 667-673
Author(s):  
Gizem Kaleli-Can ◽  
Elvan Hortaç-İştar ◽  
Hatice Ferda Özgüzar ◽  
Mehmet Mutlu ◽  
Hasan Cenk Mirza ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Plasma Polymerization ◽  
Candida Biofilm

scholarly journals Prevention of Candida biofilm formation over polystyrene by plasma polymerization technique – CORRIGENDUM

2020 ◽  
Vol 10 (4) ◽  
pp. 704-704
Author(s):  
Gizem Kaleli-Can ◽  
Elvan Hortaç-İştar ◽  
Hatice Ferda Özgüzar ◽  
Mehmet Mutlu ◽  
Hasan Cenk Mirza ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Plasma Polymerization ◽  
Candida Biofilm

scholarly journals The Effect of Zuccagnia punctata, an Argentine Medicinal Plant, on Virulence Factors from Candida Species

2014 ◽  
Vol 9 (7) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Nuño Gabriela ◽  
Alberto María Rosa ◽  
Zampini Iris Catiana ◽  
Cuello Soledad ◽  
Ordoñez Roxana Mabel ◽  
...  
Keyword(s):  
Virulence Factors ◽  
Candida Species ◽  
Biofilm Formation ◽  
Germ Tube ◽  
Fungal Infections ◽  
Tube Formation ◽  
Planktonic Cells ◽  
Invasive Potential ◽  
Invasion Mechanisms ◽  
Candida Biofilm

Zuccagnia punctata Cav. has been used as a traditional medicine in Argentina for the treatment of bacterial and fungal infections. In this study, we evaluated the ability of Z. punctata extract (ZpE) and compounds isolated from it to inhibit the growth and virulence factors of Candida species. ZpE showed inhibitory activity against planktonic cells of all assayed Candida species with MIC values of 400 μg/mL and with MFC values between 400 and 1,200 μg/mL. The principal identified compounds by HPLC-MS/MS and UV-VIS were chalcones (2′,4′-dihydroxy-3′-methoxychalcone, 2′,4′- dihydroxychalcone), flavones (galangin, 3,7-dihydroxyflavone and chrysin) and flavanones (naringenin, 7-hydroxyflavanone and pinocembrine). These compounds were more effective as inhibitors than the extracts upon biofilm formation as well as on preformed Candida biofilm and yeast germ tube formation. Furthermore, ZpE and chalcones are able to inhibit exoenzymes, which are responsible for the invasion mechanisms of the pathogens. All these effects could moderate colonization, thereby suppressing the pathogen invasive potential. Our results indicate that ZpE and chalcones could be used in antifungal therapy.

scholarly journals Effects of extracellular DNA from Candida albicans and pneumonia-related pathogens on Candida biofilm formation and hyphal transformation

2014 ◽  
Vol 116 (6) ◽  
pp. 1531-1542 ◽  
Author(s):  
B. Sapaar ◽  
A. Nur ◽  
K. Hirota ◽  
H. Yumoto ◽  
K. Murakami ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Biofilm Formation ◽  
Extracellular Dna ◽  
Candida Biofilm

scholarly journals Effect of Clove and Thyme Essential Oils on Candida Biofilm Formation and the Oil Distribution in Yeast Cells

Molecules ◽  
2019 ◽  
Vol 24 (10) ◽  
pp. 1954 ◽  
Author(s):  
Katarzyna Rajkowska ◽  
Paulina Nowicka-Krawczyk ◽  
Alina Kunicka-Styczyńska
Keyword(s):  
Essential Oils ◽  
Biofilm Formation ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Yeast Cells ◽  
Confocal Laser ◽  
Minimal Inhibitory Concentrations ◽  
Candida Sp ◽  
A Cell ◽  
Candida Biofilm

Candida biofilm structure is particularly difficult to eradicate, since biofilm is much more resistant to antifungal agents than planktonic cells. In this context, a more effective strategy seems to be the prevention of biofilm formation than its eradication. The aim of the study was to examine whether the process of initial colonization of materials (glass, polyethylene terephthalate, polypropylene) by food-borne Candida sp. can be impeded by clove and thyme essential oils, used at their minimal inhibitory concentrations. In the presence of clove oil, 68.4–84.2% of the yeast tested showed a statistically significant reduction in biofilm formation, depending on the material. After treatment with thyme oil, statistically significant decrease in biofilm cell numbers was observed for 63.2–73.7% of yeasts. Confocal laser scanning microscopy showed diverse compounds of clove and thyme oils that were disparately located in C. albicans cell, on a cell wall and a cell membrane, in cytoplasm, and in vacuoles, depicting the multidirectional action of essential oils. However, essential oils that were used in sub-inhibitory concentration were sequestrated in the yeast vacuoles, which indicate the activation of Candida defense mechanisms by cell detoxification. Clove and thyme essential oils due to their anti-biofilm activity can be efficiently used in the prevention of the tested abiotic surfaces colonization by Candida sp.

The Effect of Resin-Based Dental Sealant Containing Vanillin on Biofilm Formation of Candida albicans

2021 ◽  
Vol 904 ◽  
pp. 277-281
Author(s):  
Boonyanit Thaweboon ◽  
Sroisiri Thaweboon
Keyword(s):  
Early Childhood ◽  
Candida Albicans ◽  
Young Children ◽  
Biofilm Formation ◽  
Early Childhood Caries ◽  
Cell Counting ◽  
Dental Sealants ◽  
Dental Sealant ◽  
Candida Biofilm ◽  
Childhood Caries

Early childhood caries (ECC), or baby bottle tooth decay is one of the most common chronic diseases that affect young children at 6 years of age or younger. The disease is resulted from complex interactions between tooth structures and cariogenic microorganisms in dental biofilm after exposure to fermentable carbohydrates through improper feeding practices. This leads to a rapid development of demineralization of teeth shortly after they erupt in the oral cavity. Candida albicans have been reported recently to be associated with the pathogenesis of ECC. One of the general approaches currently used for the prevention of ECC is the application of dental sealant to occlusal (chewing surface) pits and fissures of caries-susceptible teeth. Many types of dental sealants have been extensively used and various antimicrobial agents were introduced to dental sealants to increase their caries preventive effect. The aim of the present study was to evaluate the antimicrobial effect of vanillin incorporated resin-based dental sealant on C. albicans biofilm. Resin-based dental sealants (Clinpro; 3M ESPE, USA) containing different concentrations of vanillin (0%, 0.5%, 1% and 5% w/v) were prepared in 96-well plate. The suspension (107 colony forming unit/mL) of each strain of Candida albicans (ATCC 10231, ATCC 18804 and 2 clinical isolates) was added to saliva-coated sealant resin samples and incubated at 37°C in 5% CO2 atmosphere for 24 h to allow the Candida biofilm formation. The quantity of vital biofilm was determined by WST Microbial Cell Counting Kit (Dojindo Molecular Technologies, USA) at 460 nm. All tests were done in triplicate and repeated three times. Kruskal-Wallis test followed by Dunn’s method were used to account for multiple comparisons. Statistical significance was set at p-value less than 0.05. The results showed that 0.5-5% vanillin-incorporated dental sealant resins showed a significant suppressive effect against all strains of Candida. No significant differences of anti-biofilm effects were found among Candida strains. Approximately 30-50% Candida biofilm reduction was noticed in sealant resins containing vanillin compared with controls without vanillin. In conclusion, the adding of vanillin (0.5-5% w/v) to dental sealant resins is effective in inhibiting biofilm formation of fungus related to early childhood caries, C. albicans. Therefore, the use of dental sealants containing vanillin has the potential to prevent this type of dental caries in young children and biofilm formation by oral Candida. Further clinical studies are required to investigate physical properties of this sealant and clinical evaluation in the patients.

scholarly journals Inhibition of biofilm formation on polystyrene substrates by atmospheric pressure plasma polymerization of siloxane‐based coatings

2021 ◽  
Author(s):  
Rodolfo Múgica‐Vidal ◽  
Elisa Sainz‐García ◽  
Ignacio Muro‐Fraguas ◽  
Ana Sainz‐García ◽  
Ana González‐Marcos ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Biofilm Formation ◽  
Plasma Polymerization ◽  
Atmospheric Pressure Plasma ◽  
Pressure Plasma

Impact of various finishing and polishing techniques and composite materials on Candida albicans biofilm formation

2019 ◽  
Vol 58 (5) ◽  
pp. 698-702
Author(s):  
Elif Aybala Oktay ◽  
Seyda Ersahan ◽  
Fidan Alakus Sabuncuoglu ◽  
Huseyin Tort ◽  
Serpil Karaoglanoglu
Keyword(s):  
Composite Material ◽  
Candida Albicans ◽  
Composite Materials ◽  
Biofilm Formation ◽  
Composite Resins ◽  
Oral Infections ◽  
Restorative Materials ◽  
Polishing Procedures ◽  
Candida Albicans Biofilms ◽  
Candida Biofilm

Abstract Candida albicans biofilms are commonly associated with severe oral infections. In dentistry, prosthetic and restorative materials are potential structures for the adhesion of C. albicans facilitating the formation of Candida biofilm and infection. Three composite resins (Charisma Classic, Sonic Fill, Estelite ∑ Quick) and two finishing-polishing systems (Biscover LV, Dental Finishing Disc) were evaluated for Candida biofilm formation. A Candida biofilm assay showed that both the resin and the finishing/polishing procedures affect Candida biofilm formation. Specifically, Candida biofilm formation was significantly lower in Sonic Fill resin than both Charisma Classic and Estelite ∑ Quick (P = .021). The type of finishing and polishing procedure also significantly affected the Candida biofilm formation to composite material (P < .001). Candida biofilm formation was more advanced after Biscover LV procedure than Dental Finishing Disc procedure.

Secretory products of Escherichia coli biofilm modulate Candida biofilm formation and hyphal development

2013 ◽  
Vol 4 (3) ◽  
pp. 186-199 ◽  
Author(s):  
H. M. H. N. Bandara ◽  
Becky P. K. Cheung ◽  
Rory M. Watt ◽  
Li Jian Jin ◽  
Lakshman P. Samaranayake
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Hyphal Development ◽  
Secretory Products ◽  
Candida Biofilm

scholarly journals Effect of serum concentration on Candida biofilm formation on acrylic surfaces

Mycoses ◽  
2000 ◽  
Vol 43 (3-4) ◽  
pp. 139-143 ◽  
Author(s):  
Nikawa ◽  
Nishimura ◽  
Makihira ◽  
Hamada ◽  
Sadamori ◽  
...  
Keyword(s):  
Serum Concentration ◽  
Biofilm Formation ◽  
Candida Biofilm
Sign in / Sign up

Export Citation Format

Share Document