scholarly journals Effects of extracellular DNA from Candida albicans and pneumonia-related pathogens on Candida biofilm formation and hyphal transformation

2014 ◽  
Vol 116 (6) ◽  
pp. 1531-1542 ◽  
Author(s):  
B. Sapaar ◽  
A. Nur ◽  
K. Hirota ◽  
H. Yumoto ◽  
K. Murakami ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Biofilm Formation ◽  
Extracellular Dna ◽  
Candida Biofilm

The Effect of Resin-Based Dental Sealant Containing Vanillin on Biofilm Formation of Candida albicans

2021 ◽  
Vol 904 ◽  
pp. 277-281
Author(s):  
Boonyanit Thaweboon ◽  
Sroisiri Thaweboon
Keyword(s):  
Early Childhood ◽  
Candida Albicans ◽  
Young Children ◽  
Biofilm Formation ◽  
Early Childhood Caries ◽  
Cell Counting ◽  
Dental Sealants ◽  
Dental Sealant ◽  
Candida Biofilm ◽  
Childhood Caries

Early childhood caries (ECC), or baby bottle tooth decay is one of the most common chronic diseases that affect young children at 6 years of age or younger. The disease is resulted from complex interactions between tooth structures and cariogenic microorganisms in dental biofilm after exposure to fermentable carbohydrates through improper feeding practices. This leads to a rapid development of demineralization of teeth shortly after they erupt in the oral cavity. Candida albicans have been reported recently to be associated with the pathogenesis of ECC. One of the general approaches currently used for the prevention of ECC is the application of dental sealant to occlusal (chewing surface) pits and fissures of caries-susceptible teeth. Many types of dental sealants have been extensively used and various antimicrobial agents were introduced to dental sealants to increase their caries preventive effect. The aim of the present study was to evaluate the antimicrobial effect of vanillin incorporated resin-based dental sealant on C. albicans biofilm. Resin-based dental sealants (Clinpro; 3M ESPE, USA) containing different concentrations of vanillin (0%, 0.5%, 1% and 5% w/v) were prepared in 96-well plate. The suspension (107 colony forming unit/mL) of each strain of Candida albicans (ATCC 10231, ATCC 18804 and 2 clinical isolates) was added to saliva-coated sealant resin samples and incubated at 37°C in 5% CO2 atmosphere for 24 h to allow the Candida biofilm formation. The quantity of vital biofilm was determined by WST Microbial Cell Counting Kit (Dojindo Molecular Technologies, USA) at 460 nm. All tests were done in triplicate and repeated three times. Kruskal-Wallis test followed by Dunn’s method were used to account for multiple comparisons. Statistical significance was set at p-value less than 0.05. The results showed that 0.5-5% vanillin-incorporated dental sealant resins showed a significant suppressive effect against all strains of Candida. No significant differences of anti-biofilm effects were found among Candida strains. Approximately 30-50% Candida biofilm reduction was noticed in sealant resins containing vanillin compared with controls without vanillin. In conclusion, the adding of vanillin (0.5-5% w/v) to dental sealant resins is effective in inhibiting biofilm formation of fungus related to early childhood caries, C. albicans. Therefore, the use of dental sealants containing vanillin has the potential to prevent this type of dental caries in young children and biofilm formation by oral Candida. Further clinical studies are required to investigate physical properties of this sealant and clinical evaluation in the patients.

Impact of various finishing and polishing techniques and composite materials on Candida albicans biofilm formation

2019 ◽  
Vol 58 (5) ◽  
pp. 698-702
Author(s):  
Elif Aybala Oktay ◽  
Seyda Ersahan ◽  
Fidan Alakus Sabuncuoglu ◽  
Huseyin Tort ◽  
Serpil Karaoglanoglu
Keyword(s):  
Composite Material ◽  
Candida Albicans ◽  
Composite Materials ◽  
Biofilm Formation ◽  
Composite Resins ◽  
Oral Infections ◽  
Restorative Materials ◽  
Polishing Procedures ◽  
Candida Albicans Biofilms ◽  
Candida Biofilm

Abstract Candida albicans biofilms are commonly associated with severe oral infections. In dentistry, prosthetic and restorative materials are potential structures for the adhesion of C. albicans facilitating the formation of Candida biofilm and infection. Three composite resins (Charisma Classic, Sonic Fill, Estelite ∑ Quick) and two finishing-polishing systems (Biscover LV, Dental Finishing Disc) were evaluated for Candida biofilm formation. A Candida biofilm assay showed that both the resin and the finishing/polishing procedures affect Candida biofilm formation. Specifically, Candida biofilm formation was significantly lower in Sonic Fill resin than both Charisma Classic and Estelite ∑ Quick (P = .021). The type of finishing and polishing procedure also significantly affected the Candida biofilm formation to composite material (P < .001). Candida biofilm formation was more advanced after Biscover LV procedure than Dental Finishing Disc procedure.

scholarly journals Extracellular DNA release confers heterogeneity in Candida albicans biofilm formation

2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Ranjith Rajendran ◽  
Leighann Sherry ◽  
David F Lappin ◽  
Chris J Nile ◽  
Karen Smith ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Biofilm Formation ◽  
Extracellular Dna ◽  
Dna Release

scholarly journals Composition and Biological Activity of Vitis vinifera Winter Cane Extract on Candida Biofilm

2021 ◽  
Vol 9 (11) ◽  
pp. 2391
Author(s):  
Zdeněk Kodeš ◽  
Maria Vrublevskaya ◽  
Markéta Kulišová ◽  
Petr Jaroš ◽  
Martina Paldrychová ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Vitis Vinifera ◽  
Metabolic Activity ◽  
Biofilm Formation ◽  
Total Phenolic ◽  
Plant Polyphenols ◽  
Microscopy Imaging ◽  
Grape Extract ◽  
Potential Source ◽  
Candida Biofilm

Vitis vinifera canes are waste material of grapevine pruning and thus represent cheap source of high-value polyphenols. In view of the fact that resistance of many pathogenic microorganisms to antibiotics is a growing problem, the antimicrobial activity of plant polyphenols is studied as one of the possible approaches. We have investigated the total phenolic content, composition, antioxidant activity, and antifungal activity against Candida biofilm of an extract from winter canes and a commercially available extract from blue grapes. Light microscopy and confocal microscopy imaging as well as crystal violet staining were used to quantify and visualize the biofilm. We found a decrease in cell adhesion to the surface depending on the concentration of resveratrol in the cane extract. The biofilm formation was observed as metabolic activity of Candida albicans, Candida parapsilosis and Candida krusei biofilm cells and the minimum biofilm inhibitory concentrations were determined. The highest inhibition of metabolic activity was observed in Candida albicans biofilm after treatment with the cane extract (30 mg/L) and blue grape extract (50 mg/L). The composition of cane extract was analyzed and found to be comparatively different from blue grape extract. In addition, the content of total phenolic groups in cane extract was three-times higher (12.75 gGA/L). The results showed that cane extract was more effective in preventing biofilm formation than blue grape extract and winter canes have proven to be a potential source of polyphenols for antimicrobial and antibiofilm treatment.

scholarly journals Eradication of Candida albicans Biofilm Viability: In Vitro Combination Therapy of Cationic Carbosilane Dendrons Derived from 4-Phenylbutyric Acid with AgNO3 and EDTA

2021 ◽  
Vol 7 (7) ◽  
pp. 574
Author(s):  
Natalia Gómez-Casanova ◽  
Tania Lozano-Cruz ◽  
Juan Soliveri ◽  
Rafael Gomez ◽  
Paula Ortega ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Medical Devices ◽  
Biofilm Formation ◽  
Second Generation ◽  
Antifungal Agents ◽  
Human Pathogen ◽  
Other Substances ◽  
Phenylbutyric Acid ◽  
Candida Biofilm

Candida albicans is a human pathogen of significant clinical relevance. This pathogen is resistant to different drugs, and most clinical antifungals are not effective against the prevention and treatment of C. albicans infections. As with other microorganisms, it can produce biofilms that serve as a barrier against antifungal agents and other substances, contributing to infection in humans and environmental tolerance of this microorganism. Thus, resistances and biofilm formation make treatment difficult. In addition, the complete eradication of biofilms in implants, catheters and other medical devices, is challenging and necessary to prevent relapses of candidemia. Therefore, it is a priority to find new molecules or combinations of compounds with anti-Candida biofilm activity. Due to the difficulty of treating and removing biofilms, the aim of this study was to evaluate the in vitro ability of different generation of cationic carbosilane dendrons derived from 4-phenylbutyric acid, ArCO2Gn(SNMe3I)m, to eradicate C. albicans biofilms. Here, we assessed the antifungal activity of the second generation dendron ArCO2G2(SNMe3I)4 against C. albicans cells and established biofilms since it managed to seriously damage the membrane. In addition, the combinations of the second generation dendron with AgNO3 or EDTA eradicated the viability of biofilm cells. Alterations were observed by scanning electron microscopy and cytotoxicity was assessed on HeLa cells. Our data suggest that the dendritic compound ArCO2G2(SNMe3I)4 could represent an alternative to control the infections caused by this pathogen.

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