Super-Critical-CO2 De-ECM Process

MRS Advances ◽  
2018 ◽  
Vol 3 (40) ◽  
pp. 2391-2397 ◽  
Author(s):  
Diana Cho ◽  
Seungwon Chung ◽  
Jaeseok Eo ◽  
Namsoo P. Kim
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Engineering Application ◽  
Biochemical Properties ◽  
The Body ◽  
Host Responses ◽  
Tissue Engineering Application ◽  
Decellularized Extracellular Matrix ◽  
Tissue Repair And Regeneration ◽  
High Degree

ABSTRACTExtracellular Matrix (ECM), a natural biomaterials, have recently garnered attention in tissue engineering for their high degree of cell proliferative capacity, biocompatibility, biodegradability, and tenability in the body. Decellularization process offers a unique approach for fabricating ECM-based natural scaffold for tissue engineering application by removing intracellular contents in a tissue that could cause any adverse host responses. The effects of Supercritical carbon dioxide (Sc-CO2) treatment on the histological and biochemical properties of the decellularized extracellular matrix (de-ECM) were evaluated and compared with de-ECM from conventional decellularization process to see if it offers significantly reduced treatment times, complete decellularization, and well preserved extracellular matrix structure. The study has shown that a novel method of using supercritical fluid extraction system indeed removed all unnecessary residues and only leaving ECM. The potential of Sc-CO2 de-ECM progressed as a promising approach in tissue repair and regeneration.

scholarly journals Polymeric Scaffolds in Tissue Engineering Application: A Review

2011 ◽  
Vol 2011 ◽  
pp. 1-19 ◽  
Author(s):  
Brahatheeswaran Dhandayuthapani ◽  
Yasuhiko Yoshida ◽  
Toru Maekawa ◽  
D. Sakthi Kumar
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Engineering Application ◽  
Biologically Active ◽  
Porous Scaffolds ◽  
Tissue Engineering Application ◽  
Tissue Engineering Scaffolds ◽  
Matrix Deposition ◽  
Bioactive Agents ◽  
Regenerate Tissue

Current strategies of regenerative medicine are focused on the restoration of pathologically altered tissue architectures by transplantation of cells in combination with supportive scaffolds and biomolecules. In recent years, considerable interest has been given to biologically active scaffolds which are based on similar analogs of the extracellular matrix that have induced synthesis of tissues and organs. To restore function or regenerate tissue, a scaffold is necessary that will act as a temporary matrix for cell proliferation and extracellular matrix deposition, with subsequent ingrowth until the tissues are totally restored or regenerated. Scaffolds have been used for tissue engineering such as bone, cartilage, ligament, skin, vascular tissues, neural tissues, and skeletal muscle and as vehicle for the controlled delivery of drugs, proteins, and DNA. Various technologies come together to construct porous scaffolds to regenerate the tissues/organs and also for controlled and targeted release of bioactive agents in tissue engineering applications. In this paper, an overview of the different types of scaffolds with their material properties is discussed. The fabrication technologies for tissue engineering scaffolds, including the basic and conventional techniques to the more recent ones, are tabulated.

scholarly journals Marine Based Biomaterials in Dental Regeneration

2020 ◽  
Vol 5 (10) ◽  
pp. 443-448
Author(s):  
Dr. Mekha Grace Varghese ◽  
Dr. Thomas George V. ◽  
Dr. Nebu George Thomas ◽  
Dr. Alenya Mary Pyas ◽  
Dr. Arimboor Maymol Francis
Keyword(s):  
Tissue Engineering ◽  
Biomedical Application ◽  
Engineering Application ◽  
The Body ◽  
Local Drug Delivery ◽  
Tissue Engineering Application ◽  
Natural Polymers ◽  
Dental Tissue ◽  
Signalling Molecules ◽  
Periodontal Tissues

The novel approach of tissue engineering aims at regenerating the functional alveolar or periodontal tissues through a series of key events that is modulated by the use of scaffolds, cells and signalling molecules. Many synthetic and natural polymers have been used as tissue engineering constructs so far with varying results in regeneration. Developing a biomaterial to replace the damaged tissue is of paramount importance for effective regeneration. Due to its rich biodiversity, marine environment yields structures with immense potential for biomedical application. These bio molecules offer many applications in cartilage and bone tissue engineering, dental tissue regeneration, wound healing and local drug delivery system. These substances are usually nontoxic, bio compatible and well tolerated by the body, which boost their efficacy for tissue engineering application. In this article, we are trying to brief the various marine based biomaterials used in dental regeneration, their possible sources and clinical applications.

scholarly journals Electrospun Nanofibers and their Application in Tissue Repair and Engineering

2020 ◽  
Author(s):  
Sareh Arjmand ◽  
Alireza Partovi Baghdadeh ◽  
Amin Hamidi ◽  
Seyed Omid Ranaei Siadat
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
High Surface ◽  
Engineering Application ◽  
Volume Ratio ◽  
Biologically Active ◽  
Dimensional Structure ◽  
Porous Scaffolds ◽  
Tissue Engineering Application ◽  
Effective Parameters

Introduction: Tissue engineering is the repair and replacement of damaged tissues and requires a combination of cells, growth factor and porous scaffolds. Scaffolds, as one of the main components in tissue engineering, are used as a template for tissue regeneration and induction and guidance of growth of the new and biologically active tissues. An ideal scaffold in tissue engineering, imitating an extracellular matrix, provides a suitable environment for adhesion, growth and cell proliferation. Scaffolds have also been used as the carriers for the controlled delivery of drugs and proteins. Variety of porous scaffolds, fabricated from biological and synthetic materials and using different manufacturing methods, have been introduced. Among them nanofibrous scaffolds have attracted great attention due to remarkable advantages including the highly porous three-dimensional structure with interconnected cavities which enable the transportation of food and waste materials, as well as high surface to volume ratio. So far, different methods and techniques have been introduced for production of scaffolds with structures similar to the extracellular matrix. Amongst them electrospinning, due to easiness and more control over effective parameters, are preferred. The present study make a review about the used materials and various methods of nanofibrous scaffold fabrication using electrospinning technology, with emphasis on the use of tissue engineering application. It also discussed about the progress and challenges ahead and the goals and perspective presented for this approach.

Coating of Laponite on PLA Nanofibrous for Bone Tissue Engineering Application

2021 ◽  
Vol 29 (3) ◽  
pp. 191-198
Author(s):  
Zahra Orafa ◽  
Shiva Irani ◽  
Ali Zamanian ◽  
Hadi Bakhshi ◽  
Habib Nikukar ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Engineering Application ◽  
Tissue Engineering Application ◽  
Bone Tissue Engineering Application

Electrospun nano-bio membrane for bone tissue engineering application- a new approach

2020 ◽  
Vol 249 ◽  
pp. 123010 ◽  
Author(s):  
Senthil Rethinam ◽  
Bahri Basaran ◽  
Sumathi Vijayan ◽  
Ali Mert ◽  
Oğuz Bayraktar ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Engineering Application ◽  
Tissue Engineering Application ◽  
New Approach ◽  
Bone Tissue Engineering Application
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