In vitro evaluation of bactericidal effect of silver and gold-silver nanoparticles coated with silicon dioxide on Xanthomonas fragariae

MRS Advances ◽  
2017 ◽  
Vol 2 (49) ◽  
pp. 2683-2688
Author(s):  
Juana-Cristina Ibarra-Arán ◽  
Douglas Rodríguez-Martínez ◽  
Eugenio Rodríguez-González ◽  
Jesús-Roberto González-Castillo

ABSTRACTIn this work it was evaluated the bactericidal effect of silver and silver-gold nanoparticles coated with silicon dioxide on Xanthomonas fragariae. Nanoparticles were synthesized by combining laser ablation and chemical synthesis techniques. Irradiating the samples at the same wavelength where nanoparticles exhibit its maximum absorbance is possible to photo induce the bactericidal effect by detonating the surface plasmon resonance. The results showed that the minimum bactericidal concentration (MBC) of silver nanoparticles was 16 μM (referred to [AgNO3]) and for silver-gold nanoparticles were 32 μM (referred to [AgNO3-HAuCl4]) at 60 minutes of irradiation.

2016 ◽  
Vol Volume 11 ◽  
pp. 5003-5014 ◽  
Author(s):  
Pei Dang Liu ◽  
Haizhen Jin ◽  
Zhirui Guo ◽  
Jun Ma ◽  
Jing Zhao ◽  
...  

Author(s):  
Thibault Frippiat ◽  
Charlotte Paindaveine ◽  
Jean-Noel Duprez ◽  
Catherine Delguste ◽  
Jacques Mainil ◽  
...  

MOMENTO ◽  
2021 ◽  
pp. 1-11
Author(s):  
Miguel A. Valverde-Alva ◽  
Jhenry F. Agreda-Delgado ◽  
Juan A. Vega-González ◽  
Juan C. Rodríguez-Soto ◽  
Julio C. Idrogo-Córdova ◽  
...  

The effect of magnetic field of 0.3 T on the concentration, distribution of sizes in suspension and zeta potential of colloidal gold and colloidal silver nanoparticles, obtained by considering the pulsed laser ablation in double distilled water was studied. The magnetic field was transverse to the direction of incidence of the laser radiation and parallel to the surface of a submerged target. An Nd: YAG laser was used (1064 nm in wavelength, 10 ns in duration, repetition rate of 10 Hz and 37 mJ of energy) to ablate targets. The colloids were characterized by inductively coupled plasma optical emission spectroscopy, ultraviolet-visible spectroscopy, dynamic light scattering and zeta potential. Concentration analysis suggested that applying magnetic field of 0.3 T during nanoparticle synthesis leads to higher concentration. Applying magnetic field led to an eleven percent increase in the concentration of the colloid with gold nanoparticles and a five percent increase in the concentration of the colloidal silver nanoparticles. The absorption spectra suggested the presence of spherical nanoparticles. When analyzing the effect of the magnetic field on the hydrodynamic size distribution of the nanoparticles and the zeta potential of the colloids, no significant changes were evidenced. The magnetic confinement of the plasma induced by laser ablation caused changes in the characteristics of the colloids.


2018 ◽  
Vol 5 (1) ◽  
pp. 171113 ◽  
Author(s):  
Valentina Marassi ◽  
Luisana Di Cristo ◽  
Stephen G. J. Smith ◽  
Simona Ortelli ◽  
Magda Blosi ◽  
...  

Silver nanoparticle-based antimicrobials can promote a long lasting bactericidal effect without detrimental toxic side effects. However, there is not a clear and complete protocol to define and relate the properties of the particles (size, shape, surface charge, ionic content) with their specific activity. In this paper, we propose an effective multi-step approach for the identification of a ‘purpose-specific active applicability window’ to maximize the antimicrobial activity of medical devices containing silver nanoparticles (Ag NPs) (such as surface coaters), minimizing any consequent risk for human health (safety by design strategy). The antimicrobial activity and the cellular toxicity of four types of Ag NPs, differing in their coating composition and concentration have been quantified. Through the implementation of flow-field flow fractionation, Ag NPs have been characterized in terms of metal release, size and shape. The particles are fractionated in the process while being left unmodified, allowing for the identification of biological particle-specific contribution. Toxicity and inflammatory response in vitro have been assessed on human skin models, while antimicrobial activity has been monitored with both non-pathogenic and pathogenic Escherichia coli . The main benefit associated with such approach is the comprehensive assessment of the maximal effectiveness of candidate nanomaterials, while simultaneously indexing their properties against their safety.


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