scholarly journals Silver nanoparticles as a medical device in healthcare settings: a five-step approach for candidate screening of coating agents

2018 ◽  
Vol 5 (1) ◽  
pp. 171113 ◽  
Author(s):  
Valentina Marassi ◽  
Luisana Di Cristo ◽  
Stephen G. J. Smith ◽  
Simona Ortelli ◽  
Magda Blosi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Specific Activity ◽  
Bactericidal Effect ◽  
Ag Nps ◽  
Healthcare Settings ◽  
Coating Agents ◽  
Human Skin Models ◽  
Specific Contribution

Silver nanoparticle-based antimicrobials can promote a long lasting bactericidal effect without detrimental toxic side effects. However, there is not a clear and complete protocol to define and relate the properties of the particles (size, shape, surface charge, ionic content) with their specific activity. In this paper, we propose an effective multi-step approach for the identification of a ‘purpose-specific active applicability window’ to maximize the antimicrobial activity of medical devices containing silver nanoparticles (Ag NPs) (such as surface coaters), minimizing any consequent risk for human health (safety by design strategy). The antimicrobial activity and the cellular toxicity of four types of Ag NPs, differing in their coating composition and concentration have been quantified. Through the implementation of flow-field flow fractionation, Ag NPs have been characterized in terms of metal release, size and shape. The particles are fractionated in the process while being left unmodified, allowing for the identification of biological particle-specific contribution. Toxicity and inflammatory response in vitro have been assessed on human skin models, while antimicrobial activity has been monitored with both non-pathogenic and pathogenic Escherichia coli . The main benefit associated with such approach is the comprehensive assessment of the maximal effectiveness of candidate nanomaterials, while simultaneously indexing their properties against their safety.

scholarly journals Facile synthesis of Silver nanoparticles with medicinal grass and its biological assessment

2019 ◽  
Author(s):  
Satheeshkumar Balu ◽  
Swetha Andra ◽  
Saranya Kannan ◽  
Manisha vidyavathy S ◽  
Murugesan Muthalagu
Keyword(s):  
Silver Nanoparticles ◽  
Bactericidal Effect ◽  
Inhibitory Effect ◽  
Biological Assessment ◽  
Ag Nps ◽  
E Coli ◽  
Mg63 Cells ◽  
Vis Spectroscopy ◽  
First Time

Biogenic synthesis of silver nanoparticles (SS-Ag NPs) using the extract of Saccharum spontaneum (SS) via green chemistry route was investigated for their in-vitro cytotoxicity on osteoblast-like cells and bactericidal effect. Synthesized SS-Ag NPs were analyzed using TEM, FTIR, XRD and UV Vis spectroscopy techniques. The biomolecules present in S. spontaneum were responsible for the reduction and capping of silver nanoparticles. A heterogenous mixture of SS-Ag NPs which consists of triangle, hexagonal, spherical and rod-like structures was obtained. Furthermore, the synthesized SS-Ag NPs were tested for in a broad spectrum of antibacterial activity against E. coli, S. aureus, K. pneumonia and P. aeruginosa and it showed significant inhibitory effect. For the first time, the enhancement in the proliferation rate of MG63 cells with SS-Ag NPs is reported.

Biosynthesis of Silver Nanoparticles by Punica Granatum Peel Extract and their Biological Activity on different Pathogenic Bacteria

2021 ◽  
Vol 19 (9) ◽  
pp. 38-45
Author(s):  
Hussein H. Al-Turnachy ◽  
Fadhilk. alibraheemi ◽  
Ahmed Abd Alreda Madhloom ◽  
Zahraa Yosif Motaweq ◽  
Nibras Yahya Abdulla
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Biological Activity ◽  
Pathogenic Bacteria ◽  
Punica Granatum ◽  
Gram Positive ◽  
Gram Negative ◽  
Inhibition Zones ◽  
Peel Extract

The present study was included the assessment of the antimicrobial activity of AgNPs synthesized by Punica granatum peel extract against pathogenic bacteria by testing warm aqueous P. granatum peel extract and silver nanoparticles. Punica granatum indicated potency for AgNP extracellular nanobiosynthesis after addition of silver nitrate (AgNO3) 4mM to the extract supernatant, in both concentrations (100mg and 50mg). The biogenic AgNPs showed potency to inhibit both gram-negative and gram-positive bacterial growth. Zons of inhibition in (mm) was lesser in gram-positive than gram-negative bacteria. The resulted phytogenic AgNPs gave higher biological activity than warm aqueous Punica granatum peel extract. The inhibition zone of the phytogenic AgNPs on E. coli reached 17.53, 22.35, and 26.06 mm at (0.1, 0.5, and 1) mg/ml respectively. While inhibition zones of Punica warm aqueous extract reached 5.33, 10.63, and 16.08 mm at the same concentrations. phytogenic AgNPs gave smaller inhibition zones in gram-positive than gram- negative. Cytotoxic activity of the phytogenic AgNPs was assayed in vitro agaist human blood erythrocytes (RBCs), spectroscopic results showed absorbance at 540 nm hemolysis was observed. In general, AgNPs showed least RBCs hemolysis percentage, at 1 mg/ml concentration, hemolysis percentage was (4.50%). This study, concluded that the Punica granatum peel extract has the power of synthses of AgNPs characterized by broad spectrum antimicrobial activity with cyto-toxicity proportional to AgNPs concentration.

Silver nanoparticles used to counter Monilinia fructicola fungicide-resistance and reduce fungicide environmental footprint 

2021 ◽  
Author(s):  
Constantinos Chrysikopoulos ◽  
Anastasios A. Malandrakis ◽  
Nektarios Kavroulakis ◽  
Anthi Stefanarou
Keyword(s):  
Silver Nanoparticles ◽  
Control Plant ◽  
Resistance Mutation ◽  
Silver Ions ◽  
Apple Fruit ◽  
Ag Nps ◽  
Gene Target ◽  
Benzimidazole Fungicides

<div><span>The potential of silver nanoparticles (Ag-NPs) to control plant pathogen <em>Monilia</em><em>fructicola </em>and to deter environmental contamination by reducing fungicide doses was evaluated <em>in vitro </em>and <em>in vivo. </em> </span>F<span>ungitoxicity screening </span>of <em><span>M. fructicola </span></em><span>isolates resulted in the detection of 18 benzimidazole-resistant (BEN-R) isolates with reduced sensitivity to fungicides  thiophanate methyl (TM)  and carbendazim. All resistant isolates caried the E198A resistance mutation in their </span><em><span>β</span>-</em>tubulin gene, target site of the benzimidazole fungicides. <span>Ag-NPs could effectively control both sensitive (BEN-S) and resistant isolates while the combination of Ag-NPs with TM significantly enhanced their fungitoxic effect both <em>in vitro </em>and in apple fruit tests. The positive correlation observed between Ag-NPs and TM+Ag-NPs treatments indicates a mixture-enhanced Ag-NPs activity/availability as a possible mechanism of synergy. No correlation between Ag-NPs  and AgNO<sub>3 </sub>could  be found suggesting difference(s) in the fungitoxic mechanism of action between Nps and their bulk/ionic counterparts. Indications of the involvement of energy (ATP) metabolism in the mode of action of Ag-NPs were also evident by the synergy observed between Ag-NPs and the </span>oxidative phosphorylation<span>-uncoupler fluazinam (FM) against both BEN-R and BEN-S phenotypes. The role of silver ions release on the inhibitory action of Ag-NPs against the fungusis probably limited since the AgNPs/NaCl combination enhanced fungitoxicity, a fact that could not be justified by the expected binding of silver with chlorine ions. Concluding, Ag-NPs can be effectively used as a means of controlling both BEN-S and BEN-R <em>M. </em><em>fructicola </em>isolates </span>while <span>their combination with conventional fungicides should aid anti-resistant strategies and reduce the environmental impact of synthetic fungicides by reducing effective doses to the control the pathogen.</span></div>

Antimicrobial activity and inhibition of biofilm formation in vitro and on human dentine by silver nanoparticles/carboxymethyl-cellulose composites

2020 ◽  
Vol 120 ◽  
pp. 104943
Author(s):  
Elizabeth Madla-Cruz ◽  
Myriam De la Garza-Ramos ◽  
César I. Romo-Sáenz ◽  
Patricia Tamez-Guerra ◽  
Marco A. Garza-Navarro ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Carboxymethyl Cellulose ◽  
Biofilm Formation ◽  
Cellulose Composites ◽  
Human Dentine

scholarly journals In Vitro Bactericidal Activity of Human β-Defensin 3 against Multidrug-Resistant Nosocomial Strains

2006 ◽  
Vol 50 (2) ◽  
pp. 806-809 ◽  
Author(s):  
Giuseppantonio Maisetta ◽  
Giovanna Batoni ◽  
Semih Esin ◽  
Walter Florio ◽  
Daria Bottai ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bactericidal Activity ◽  
Stenotrophomonas Maltophilia ◽  
Bactericidal Effect ◽  
Multidrug Resistant ◽  
Bacterial Strains ◽  
Gram Negative

ABSTRACT The antimicrobial activity of human β-defensin 3 (hBD-3) against multidrug-resistant clinical isolates of Staphylococcus aureus, Enterococcus faecium, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Acinetobacter baumannii was evaluated. A fast bactericidal effect (within 20 min) against all bacterial strains tested was observed. The presence of 20% human serum abolished the bactericidal activity of hBD-3 against gram-negative strains and reduced the activity of the peptide against gram-positive strains.

scholarly journals Synthesis and biochemical characterization of silver nanoparticles grafted chitosan (Chi-Ag-NPs): in vitro studies on antioxidant and antibacterial applications

2020 ◽  
Vol 2 (4) ◽  
Author(s):  
Pavan Kumar Dara ◽  
R. Mahadevan ◽  
P. A. Digita ◽  
S. Visnuvinayagam ◽  
Lekshmi R. G. Kumar ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Biochemical Characterization ◽  
In Vitro Studies ◽  
Ag Nps

Thermo-chemical induced production of silver nanoparticles (Ag-NPs) and their antimicrobial activity towards human pathogens

2017 ◽  
Vol 10 (5) ◽  
pp. 1322
Author(s):  
S. Krishnakumar ◽  
R. Divya ◽  
N.R. Kanchana Devi ◽  
G. Keerthana ◽  
A. Ancy Judi
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Human Pathogens ◽  
Ag Nps

scholarly journals Silver nanoparticles induce the cardiomyogenic differentiation of bone marrow derived mesenchymal stem cells via telomere length extension

2021 ◽  
Vol 12 ◽  
pp. 786-797
Author(s):  
Khosro Adibkia ◽  
Ali Ehsani ◽  
Asma Jodaei ◽  
Ezzatollah Fathi ◽  
Raheleh Farahzadi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Bone Marrow ◽  
Silver Nanoparticles ◽  
Mesenchymal Stem Cells ◽  
Telomere Length ◽  
Cardiac Markers ◽  
Ag Nps ◽  
Cardiomyogenic Differentiation

Finding new strategies for the treatment of heart failures using stem cells has attracted a lot of attention. Meanwhile, nanotechnology-based approaches to regenerative medicine hypothesize a possible combination of stem cells and nanotechnology in the treatment of diseases. This study aims to investigate the in vitro effect of silver nanoparticles (Ag-NPs) on the cardiomyogenic differentiation of bone marrow-derived mesenchymal stem cells (BM-MSCs) through detection of cardiac markers. For this purpose, MSCs were isolated from bone marrow resident and differentiated to the cardiac cells using a dedicated medium with Ag-NPs. Also, the cardiomyogenic differentiation of BM-MSCs was confirmed using immunocytochemistry. Then, real-time PCR and western blotting assay were used for measuring absolute telomere length (TL) measurement, and gene and protein assessment of the cells, respectively. It was found that 2.5 µg/mL Ag-NPs caused elongation of the telomeres and altered VEGF, C-TnI, VWF, SMA, GATA-4, TERT, and cyclin D protein and gene expression in the cardiomyogenically differentiated BM-MSCs. Also, there was a significant increase in the protein and gene expression of Wnt3 and β-catenin as main components of pathways. We concluded that Ag-NPs could change the in vitro expression of cardiac markers of BM-MSCs via the Wnt3/β-catenin signaling pathway.

scholarly journals Silver Nanoparticles: Green Synthesis, Characterization, Blood Compatibility and Protoscolicidal Efficacy against Echinococcus granulosus

2021 ◽  
Vol 41 (03) ◽  
pp. 393-399
Author(s):  
Parwin Jalal Jalil
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Silver Nanoparticles ◽  
Echinococcus Granulosus ◽  
Blood Compatibility ◽  
Ag Nps ◽  
X Ray ◽  
Hydatid Fluid ◽  
Scanning Electron

Spillage of protoscoleces within hydatid fluid during surgery for hydatid cyst is the main reason for its recurrence. Therefore, to inactivate the protoscoleces, various scolicidal substances have been tested. However, novel and more efficient agents are needed owing to several associated complications. This study focused on the effects of green synthetic Silver Nanoparticles (AgNPs) from Zizyphus spina- christi leaves on Echinococcus granulosus protoscoleces. Also, to evaluate the blood compatibility of Ag NPs. The Ag NPs were identified by ultraviolet-visible (UV-Visible) spectrophotometer, X-ray diffraction (XRD), Scanning electron microscopy imaging, and Energy-dispersive X-ray spectroscopy (EDX). Hydatid fluid was aspirated aseptically from cysts of infected sheep liver. The protoscoleces were exposed to Ag NPs at several concentrations. Also, scanning electron microscopy for ultrastructural changes and in vitro erythrocytes lysis was performed. The Ag NPs were spherical; the particles' size reached 50 nm, and presented a surface plasmon peak around 460 nm. The current study's findings indicated the powerful in vitro scolicidal efficacy of the green biosynthesized AgNPs. Several morphological alterations were observed on the protoscoleces by optical and scanning electron microscopy. Lysis of RBCs at different doses of Ag NPs was significantly (P≤0.05) less than the positive control value, thus proposing its biocompatibility. This work suggests that chemicals like polyphenols present in the extract of Z. spina- christi act as reducing and stabilizers agents to create Ag NPs Nevertheless, further investigations are needed to investigate the Ag NPs scolicidial effects in animal models.

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