The Influence of Metalized Graphene Oxide/Reduced Graphene Oxide and Sulfonated Polystyrene on Dental Pulp Stem Cell Differentiation and Protein Adsorption

MRS Advances ◽  
2017 ◽  
Vol 2 (19-20) ◽  
pp. 1059-1070 ◽  
Author(s):  
Rachel Sacks ◽  
Gila Schein ◽  
Rebecca Isseroff ◽  
Vincent Ricotta ◽  
Marcia Simon ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Stem Cell ◽  
Protein Adsorption ◽  
Dental Pulp ◽  
Stem Cell Differentiation ◽  
Sulfonated Polystyrene ◽  
Reduced Graphene ◽  
Human Dental Pulp ◽  
Osteogenic Induction ◽  
Cell Growth And Differentiation

ABSTRACTHuman dental pulp stem cells (DPSCs) can differentiate, showing potential for regenerative medicine. Designing artificial surfaces with properties appropriate for the initiation of extracellular matrix (ECM) adsorption and organization is a critical step in tissue engineering and can greatly impact protein adhesion. Sulfonated polystyrene (SPS), used as a scaffold for tissue development, stimulates protein adsorption due to the increased negative charge of sulfonate.Graphene and graphene oxide (GO) sheets enhance stem cell growth and differentiation because they are soft membranes with “high in-plane” stiffness and have the potential to be transferable and implantable platforms. This project functionalized GO and reduced GO (RGO) with gold or silver nanoparticles, mixing with SPS to investigate their combined impact on DPSC differentiation and protein adsorption, hypothesizing that this combination supplies more charges to better absorb the proteins to the surface and stimulate differentiation.Results indicate that proteins of cells plated on the gold-RGO/SPS surfaces were the most highly adsorbed and most densely packed. Additionally, the cell moduli data indicated that the metal-RGO solutions substantially induced a change in modulus even more than Dexamethasone, a glucocortoid known to enhance this process in DPSCs. This suggests that the metal-RGO solutions may be instrumental in osteogenic induction.

scholarly journals Etched 3D-Printed Polycaprolactone Constructs Functionalized with Reduced Graphene Oxide for Enhanced Attachment of Dental Pulp-Derived Stem Cells

Pharmaceutics ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2146
Author(s):  
Austin J. Bow ◽  
Thomas J. Masi ◽  
Madhu S. Dhar
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Stem Cell ◽  
Reduced Graphene Oxide ◽  
Dental Pulp ◽  
Fluorescent Protein ◽  
Acetic Acid Solution ◽  
Cell Populations ◽  
Reduced Graphene

A core challenge in the field of tissue engineering is the ability to establish pipeline workflows for the design and characterization of scaffold technologies with clinically translatable attributes. The parallel development of biomaterials and stem cell populations represents a self-sufficient and streamlined approach for establishing such a pipeline. In the current study, rat dental pulp stem cell (rDPSC) populations were established to assess functionalized polycaprolactone (PCL) constructs. Initial optimization and characterization of rDPSC extraction and culture conditions confirmed that cell populations were readily expandable and demonstrated surface markers associated with multi-potency. Subset populations were transduced to express DsRed fluorescent protein as a mechanism of tracking both cells and cell-derived extracellular matrix content on complex scaffold architecture. Thermoplastic constructs included reduced graphene oxide (rGO) as an additive to promote cellular attachment and were further modified by surface etching a weak acetic acid solution to roughen surface topographical features, which was observed to dramatically improve cell surface coverage in vitro. Based on these data, the modified rGO-functionalized PCL constructs represent a versatile platform for bone tissue engineering, capable of being applied as a standalone matrix or in conjunction with bio-active payloads such as DPSCs or other bio-inks.

scholarly journals A Mini Review Focused on the Recent Applications of Graphene Oxide in Stem Cell Growth and Differentiation

2018 ◽  
Author(s):  
Alexander Halim ◽  
Qing Luo ◽  
Yang Ju ◽  
Guanbin Song
Keyword(s):  
Stem Cells ◽  
Graphene Oxide ◽  
Stem Cell ◽  
Cell Growth ◽  
Disease Modeling ◽  
Biological Effects ◽  
Stem Cell Differentiation ◽  
Differentiation Potential ◽  
The World ◽  
Cell Growth And Differentiation

Stem cells are undifferentiated cells which can give rise to any types of cells in our body. Hence, they have been utilized for various applications such as drug testing and disease modeling. However, for the successful of those applications, the survival and differentiation of stem cells into specialized lineages should be well controlled. Growth factors and chemical agents are the most common signals to promote the proliferation and differentiation of stem cells. However, those approaches holds several drawbacks such as the negative side effects, degradation or denaturation, and expensive. To address such limitations, nanomaterials have been recently used as a better approach for controlling stem cells behaviors. Graphene oxide is the derivative of graphene, the first 2D materials in the world. Recently, due to its extraordinary properties and great biological effects on stem cells, many scientists around the world have utilized graphene oxide to enhance the differentiation potential of stem cells. In this mini review, we highlight the key advances about the effects of graphene oxide on controlling stem cell growth and various types of stem cell differentiation. We also discuss the possible molecular mechanisms of graphene oxide in controlling stem cell growth and differentiation.

scholarly journals A Mini Review Focused on the Recent Applications of Graphene Oxide in Stem Cell Growth and Differentiation

Nanomaterials ◽  
2018 ◽  
Vol 8 (9) ◽  
pp. 736 ◽  
Author(s):  
Alexander Halim ◽  
Qing Luo ◽  
Yang Ju ◽  
Guanbin Song
Keyword(s):  
Stem Cells ◽  
Graphene Oxide ◽  
Stem Cell ◽  
Cell Growth ◽  
Disease Modeling ◽  
Biological Effects ◽  
Stem Cell Differentiation ◽  
Differentiation Potential ◽  
The World ◽  
Cell Growth And Differentiation

Stem cells are undifferentiated cells that can give rise to any types of cells in our body. Hence, they have been utilized for various applications, such as drug testing and disease modeling. However, for the successful of those applications, the survival and differentiation of stem cells into specialized lineages should be well controlled. Growth factors and chemical agents are the most common signals to promote the proliferation and differentiation of stem cells. However, those approaches holds several drawbacks such as the negative side effects, degradation or denaturation, and expensive. To address such limitations, nanomaterials have been recently used as a better approach for controlling stem cells behaviors. Graphene oxide is the derivative of graphene, the first two-dimensional (2D) materials in the world. Recently, due to its extraordinary properties and great biological effects on stem cells, many scientists around the world have utilized graphene oxide to enhance the differentiation potential of stem cells. In this mini review, we highlight the key advances about the effects of graphene oxide on controlling stem cell growth and various types of stem cell differentiation. We also discuss the possible molecular mechanisms of graphene oxide in controlling stem cell growth and differentiation.

scholarly journals Graphene: A Versatile Carbon-Based Material for Bone Tissue Engineering

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Nileshkumar Dubey ◽  
Ricardo Bentini ◽  
Intekhab Islam ◽  
Tong Cao ◽  
Antonio Helio Castro Neto ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Stem Cell ◽  
Cell Attachment ◽  
High Surface ◽  
High Surface Area ◽  
Reduced Graphene ◽  
Proliferation And Differentiation ◽  
Cell Growth And Differentiation ◽  
Large Bone ◽  
Carbon Based

The development of materials and strategies that can influence stem cell attachment, proliferation, and differentiation towards osteoblasts is of high interest to promote faster healing and reconstructions of large bone defects. Graphene and its derivatives (graphene oxide and reduced graphene oxide) have received increasing attention for biomedical applications as they present remarkable properties such as high surface area, high mechanical strength, and ease of functionalization. These biocompatible carbon-based materials can induce and sustain stem cell growth and differentiation into various lineages. Furthermore, graphene has the ability to promote and enhance osteogenic differentiation making it an interesting material for bone regeneration research. This paper will review the important advances in the ability of graphene and its related forms to induce stem cells differentiation into osteogenic lineages.

Glycometabolic reprogramming associated with the initiation of human dental pulp stem cell differentiation

2015 ◽  
Vol 40 (3) ◽  
pp. 308-317 ◽  
Author(s):  
Linyan Wang ◽  
Li Cheng ◽  
Huning Wang ◽  
Hongying Pan ◽  
Hui Yang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Dental Pulp ◽  
Stem Cell Differentiation ◽  
Human Dental Pulp ◽  
Dental Pulp Stem Cell
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