Antimicrobial Activity of TiO2 Coatings Prepared by Direct Thermophoretic Deposition of Flame-Synthesized Nanoparticles

MRS Advances ◽  
2016 ◽  
Vol 2 (28) ◽  
pp. 1493-1498 ◽  
Author(s):  
Gianluigi De Falco ◽  
Amalia Porta ◽  
Pasquale Del Gaudio ◽  
Mario Commodo ◽  
Patrizia Minutolo ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Protective Coatings ◽  
Colorimetric Assay ◽  
Antimicrobial Properties ◽  
Titania Nanoparticles ◽  
Rotating Disc ◽  
Step Method ◽  
One Step ◽  
Anatase Nanoparticles

ABSTRACTThis study reports the development of a one-step method for the production of antimicrobial protective coatings for aluminum surfaces with titania nanoparticles. An aerosol flame synthesis system was used to produce monodisperse, ultra-fine TiO2 nanoparticles, which were directly deposited by thermophoresis onto plates of aluminum alloy by means of a rotating disc. Fuel-lean reactor conditions were used to synthesize pure anatase nanoparticles of 3.5 nm in diameter. Substrates were mounted onto the rotating disc that repetitively passes through the flame. Convection due to the rotational motion cooled the substrates, on which particles were deposited as films by thermophoresis. Such a system allowed to obtain submicron coatings of different thickness, by varying the total time of deposition. The antimicrobial activity of TiO2 coatings was tested against the Gram positive bacterium Staphylococcus aureus. To determine the inhibition of biofilms formation, microbes were plated on TiO2 coatings and a semi-quantitative colorimetric assay was performed using crystal violet. The tests showed that the TiO2 coating obtained with tdes=10 s inhibits up to 70-80% Staphylococcus aureus biofilm formation, and the inhibition of biofilms formation was confirmed by means of Scanning Electron Microscopy observation. Also, the antimicrobial properties of the coatings was enhanced by irradiating the samples in the UV region. The results of the present work are promising for using titania films as protective coatings for applications where an antimicrobial activity is required.

scholarly journals Antimicrobial Activity of Cultivable Endophytic and Rhizosphere Fungi Associated with “Mile-a-Minute,” Mikania cordata (Asteraceae)

2020 ◽  
Vol 2020 ◽  
pp. 1-7 ◽  
Author(s):  
Pavithra L. Jayatilake ◽  
Helani Munasinghe
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Pharmaceutical Products ◽  
Diffusion Method ◽  
Molecular Characteristics ◽  
Disk Diffusion Method ◽  
Rhizosphere Fungi

Endophytic and rhizosphere fungi are understood to be aiding the host plant to overcome a range of biotic and abiotic stresses (nutrition depletion, droughts, etc.) hence, they remain to be reservoirs of plethora of natural products with immense use. Consequently, this investigation of endophytic and rhizosphere fungi isolated from Mikania cordata (a perennial vine that is well established in Sri Lanka) for their antimicrobial properties was performed with the aim of future derivation of potential beneficial pharmaceutical products. Leaves, twigs, and roots of M. cordata were utilized to isolate a total of 9 endophytic fungi out of which the highest amount (44%) accounted was from the twigs. A sample of the immediate layer of soil adhering to the root of M. cordata was utilized to isolate 15 rhizosphere fungi. Fusarium equiseti and Phoma medicaginis were endophytes that were identified based on colony and molecular characteristics. The broad spectrum of antimicrobial activity depicted by F. equiseti (MK517551) was found to be significantly greater (p≤0.05, inhibitory against Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 25853) than P. medicaginis (MK517550) (inhibitory against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 25853) as assessed using the Kirby-Bauer disk diffusion method. Trichoderma virens and Trichoderma asperellum were rhizospere fungi that exhibited remarkable antimicrobial properties against the test pathogens chosen for the study. T. asperellum indicated significantly greater bioactivity against all four bacterial pathogens and Candida albicans ATCC 10231 under study. The ranges of minimum inhibitory concentrations (MICs) of the fungi depicting antimicrobial properties were determined. The results obtained suggest that F. equiseti, P. medicaginis, T. asperellum, and T. virens of M. cordata harness bioprospective values as natural drug candidates. This is the first report on isolation and evaluation of the antimicrobial properties of endophytic and rhizosphere fungi of Mikania cordata.

scholarly journals Antimicrobial activity of root canal filling materials

2021 ◽  
Vol 7 (1) ◽  
pp. 01-05
Author(s):  
Jikia Maia
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Antimicrobial Properties ◽  
Root Canal Filling ◽  
Root Canals ◽  
Filling Materials ◽  
Zinc Oxide Eugenol ◽  
Test Culture

The antimicrobial activity of filling materials was studied to improve the quality of treatment of complicated caries in vitro. Resorcin-formalin cement Resodont (“Latus”, Ukraine) - № 1; Endocort zinc oxide-eugenol cement (“Latus”, Ukraine) - No. 2; materials based on zinc oxide eugenol cement with dexamethasone: Endomethasone ivory (Septodont, France) - No. 3 and Endofil ("Produit Dentaires SA", Switzerland) - No. 4. To assess the antimicrobial activity of the studied materials, test microorganism strains were used: Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 6783, Candida albicans ATCC 885-653. As a result of the study, it was established that filling materials have different antimicrobial activity. Resodont has the greatest antimicrobial activity on the test culture. Endomethason is active in relation to the test cultures studied, inferior to Resodont in its antimicrobial properties. Endocort and Endofil have less antimicrobial activity than Resodont and Endomethason, but have approximately equal growth retardation rates. It should be noted that with respect to Staphylococcus aureus, Endocort was found to be almost 1.5 times more active than Endofil. The conducted studies confirm that the materials for filling of root canals by Resodont and Endocort (Latus, Ukraine) have a pronounced antibacterial effect. They are competitive with foreign sealers and are the materials of choice, having a significantly lower cost.

scholarly journals Antimicrobial activity of antiseptics in the prevention of postoperative infectious complications

2021 ◽  
pp. 33-36
Author(s):  
N. A. Bagnyuk ◽  
O. A. Nazarchuk ◽  
Y. M. Babina ◽  
R. M. Chornopyshchuk ◽  
A. V. Kulyk
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Bactericidal Effect ◽  
Infectious Complications ◽  
Antimicrobial Efficacy ◽  
Gram Positive ◽  
Gram Negative ◽  
Clinical Strains ◽  
Enterococcus Spp

Recently, among hospital strains of microorganisms, an increase in the number of antiseptic-resistant strains of opportunistic pathogens has been registered, which significantly affects the effectiveness of these drugs. It is important to study their antimicrobial efficacy to justify rational use. The aim is to conduct a comparative study of the antimicrobial efficacy of antiseptics of decamethoxine, chlorhexidine, polyhexanide. During study we examined the antimicrobial activity against 186 clinical strains of microorganisms (Acinetobacter baumannii, Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus spp., Enterobacter spp.) isolated from patients with infectious complications in the postoperative period. The minimum inhibitory (MIC) and bactericidal concentrations (MBC) of 0.02 % and 0.1 % decamethoxine, 0.05 % chlorhexidine bigluconate, 0.1 % polyhexanide were determined; antimicrobial efficacy of drugs was evaluated by the index of antiseptic activity by conventional methods. The study found high antimicrobial properties of decamethoxine, chlorhexidine, which had a high bactericidal effect on clinical strains of S. aureus, Enterococcus spp., Enterobacter spp. Proved the benefits of antimicrobial activity of the drug based on decamethoxine (p<0.001). The polyhexanide has pronounced antimicrobial properties against A. baumannii, bacteria of the family Enterobactericae, P. aeruginosa. Thus, the leading gram-positive (Staphylococcus aureus, enterococci) and gram-negative pathogens (enterobacteria, acinetobacteria, pseudomonads) are sensitive to polyhexanide, chlorhexidine and the domestic drug decamethoxin, with a probable advantage of the antimicrobial properties of the latter over all gram-positive and most gram-negative microorganisms.

scholarly journals UV Curable Coatings Based on Urethane Acrylates Containing Eugenol and Evaluation of Their Antimicrobial Activity

Coatings ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1556
Author(s):  
Paulina Bednarczyk ◽  
Agnieszka Wróblewska ◽  
Agata Markowska-Szczupak ◽  
Paula Ossowicz-Rupniewska ◽  
Małgorzata Nowak ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Protective Coatings ◽  
Antimicrobial Properties ◽  
Uv Curing ◽  
Industrial Applications ◽  
High Reactivity ◽  
Uv Curable ◽  
Gram Positive Bacteria ◽  
Wide Range ◽  
Short Time

This work presents studies on the obtaining of UV-curable coatings with antimicrobial activity. Urethane acrylates (UAs) have excellent physicochemical properties including high reactivity in systems with radical photoinitiators in the presence of UV radiation and good balance between hardness and flexibility in the formed coatings. At the same time, eugenol is well known as the compound hindering the growth of various microorganisms. Hence, the materials obtained by the modification of UA resins with eugenol can be used to protect various surfaces, especially against microorganisms. This study aimed to examine the influence of the amount of eugenol on the chemical, physical, thermal, and mechanical properties of the obtained UA coatings and find the conditions at which the optimal properties for industrial applications such coatings can be achieved. These materials were successfully obtained. Taking into account that eugenol is a very cheap reactant, and it can be obtained from natural sources by the simple distillation method, the proposed method combined the good points of obtaining protective coatings by UV curing with the utilization of vegetable, renewable reactants (biomass), such as components giving special properties to these materials, in this case, antimicrobial properties. In this study, photoreactive coatings with antimicrobial properties for the following microorganisms: fungi (C. albicans), Gram-positive bacteria (S. epidermidis) as well as Gram-negative bacteria (E. coli), were obtained. The obtained coatings were cured over a short time. They were colorless and characterized by a wide range of properties and applications.

scholarly journals Complete Genome Sequence of the Methicillin-Resistant Staphylococcus aureus Strain SQL1/USA300, Used for Testing the Antimicrobial Properties of Clay Phyllosilicates and Customized Aluminosilicates

2021 ◽  
Vol 10 (45) ◽  
Author(s):  
Enrique G. Medrano ◽  
Shelley E. Haydel
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antimicrobial Properties ◽  
Aureus Strain ◽  
Positive Bacterium ◽  
Methicillin Resistant ◽  
Content Type ◽  
Factors Associated ◽  
Critical Knowledge

Methicillin-resistant Staphylococcus aureus (MRSA) is a Gram-positive bacterium that causes community-acquired and health care-acquired infections. We previously demonstrated that clay phyllosilicates and customized aluminosilicates display antimicrobial activity against the MRSA strain SQL1. The SQL1 annotated genome reveals a USA300 lineage and contributes critical knowledge of the MRSA virulence factors associated with tissue infection.

scholarly journals FORMULATION, CHARACTERIZATION AND DETERMINATION OF ANTIBACTERIAL ACTIVITY OF POMEGRANATE (Punicagranatum) GEL.

2015 ◽  
Vol 7 ◽  
pp. 13
Author(s):  
Afendi Dahlan ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antimicrobial Properties ◽  
Pathogenic Microorganisms ◽  
Wide Range ◽  
Common Skin

revious studies have shown that Punicagranatum (P. granatum) extract exhibited antimicrobial activity against a wide range of pathogenic microorganisms. Thus, the aim of study was to formulate and determine the effectiveness of antimicrobial properties of gel containing P. granatummethanol extract against selected common skin pathogens including Staphylococcus aureus, Staphylococcusepidermidis, Pseudomonas aeruginosaand Candidaalbicans.

scholarly journals Antimicrobial Properties of 6-Bromoeugenol and Eugenol

2016 ◽  
Vol 53 ◽  
pp. 57-64
Author(s):  
Radia Mahboub ◽  
Faiza Memmou
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Inhibitory Concentration ◽  
Antimicrobial Properties ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Bacterial Strains ◽  
Disc Diffusion Method ◽  
Modest Activity

We have studied the antimicrobial properties of 6-bromoeugenol and eugenol by three strains:Pseudomonas aeruginosa(S1),Escherichia coli(S2) andStaphylococcus aureus(S3). We have determined the minimum inhibitory concentration (MIC) for a range of concentrations using the disc diffusion method. We note that all samples present an antimicrobial activity toward the tested bacterial strains at different concentrations (1, 0.5 and 0.25 mg/ml). The 6-bromoeugenol gives modest activity with (S1) and (S3). Eugenol reacts positively with thePseudomonas aeruginosa(S1) at all concentrations and with theEscherichiacoli(S2) at 0.5 mg/ml. We remark that thePseudomonas aeruginosa(S1) is the more sensitive strain thanEscherichiacoli(S2) andStaphylococcus aureus(S3). We have estimated the activity coefficient which has confirmed the antimicrobial activity of the different samples. So, 6-bromoeugenol has shown his efficiency as antimicrobial agent.

scholarly journals Antimicrobial properties of ZnO nanoparticles incorporated in polyurethane varnish

2011 ◽  
Vol 5 (1) ◽  
pp. 41-45 ◽  
Author(s):  
Dusan Zvekic ◽  
Vladimir Srdic ◽  
Maja Karaman ◽  
Milan Matavulj
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Zno Nanoparticles ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Plate Test ◽  
Sacharomyces Cerevisiae

The antimicrobial effect of the ZnO nanoparticles dispersed in polyurethane varnishes was investigated. Antimicrobial activity was evaluated against three bacteria (Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa) and one strain fungi (Sacharomyces cerevisiae) by the 'pour-plate' test. It was shown that the ZnO nanoparticles efficiently inhibit growth of the colonies of Staphylococcus aureus, Pseudomonas aeruginosa and Saccharomyces cerevisiae, but their activity against Escherichia coli was found to be questionable suggesting more detailed research.

Preparation, Characterization of Phosphorus Doped Titania Photocatalysts with High Photocatalystic Properties

2010 ◽  
Vol 113-116 ◽  
pp. 2154-2157 ◽  
Author(s):  
Si Yao Guo ◽  
Jin Bing Sun ◽  
Feng Lu Wang ◽  
Lin Yang ◽  
Feng Zhang ◽  
...  
Keyword(s):  
Photoelectron Spectroscopy ◽  
Titania Nanoparticles ◽  
Molar Ratio ◽  
X Ray Diffraction ◽  
Step Method ◽  
X Ray ◽  
One Step ◽  
Titania Photocatalyst ◽  
Calcination Method ◽  
Doped Titania

Phosphor-doped titania nanoparticles were synthesized by a one step method, which were prepared by conventional calcination method. These samples have much higher photocatalytic activity for methylene blue degradation. The resulting materials were characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), etc. Moreover, we use high P/TiO2 molar ratio to get the most suitable proportion for the synthesis of P-doped titania photocatalyst.

scholarly journals GC/MS Analysis and Antibacterial Activity of the Essential Oil of Moroccan Tetraclinis articulata (Vahl) Masters

2019 ◽  
Vol 8 (4) ◽  
pp. 302-307 ◽  
Author(s):  
Halima Rabib ◽  
Sophia Zougagh ◽  
Mohamed Hsain ◽  
Wadi Badri ◽  
Tayeb Koussa
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Antimicrobial Properties ◽  
Bornyl Acetate ◽  
Average Yield ◽  
Mountain Area ◽  
Plain Area ◽  
Tetraclinis Articulata

The quality and the variability of the chemical composition and antimicrobial properties of essentials oils isolated from Tetraclinis articulata (Vahl) leaves, which were studied from two different regions that are ecologically various. The average yield of essential oil is 0,56 % for the Ras Elma Tazekka region (mountain) and 0,36% for the Debdou region (plain). An analysis made by GC and GC/ MS identified 22 components in Tetraclinis articulata leaves essential oil in the mountain area and 29 components in the plain area. These major components represent the variability of quantity. The percentages following respectively by the two areas are: Bornyl acetate (34,84%; 32,55%), α-Pinene (11,41%; 18,83%), Camphor (11,24 %; 11,31%), Limonene (11,94 %; 8%) and Borneol (8,35%; 7,79%). The antimicrobial activity of T. articulata essential oil was tested against clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli which has been inhibited from the 50μg / mL concentration for the Debdou region (Plain) and 25μg / mL for that of the Ras Elma Tazekka region (Mountain).

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