In vivo stability of protein coatings on poly lactic co glycolic nanoparticles

MRS Advances ◽  
2016 ◽  
Vol 1 (56) ◽  
pp. 3767-3773 ◽  
Author(s):  
Jordi Llop ◽  
Marco Marradi ◽  
Pengfei Jiang ◽  
Vanessa Gómez-Vallejo ◽  
Zuriñe Baz ◽  
...  
Keyword(s):  
Electrophilic Substitution ◽  
Single Photon ◽  
Photon Emission ◽  
Degradation Process ◽  
Energy Spectra ◽  
Single Photon Emission ◽  
Computerised Tomography ◽  
The Core ◽  
Wide Range

ABSTRACTSubmicron-sized poly(lactide-co-glycolide) nanoparticles (PLGA-NPs) stabilised with bovine serum albumin (BSA) are dual radiolabelled using gamma emitters with different energy spectra incorporated into the core and coating. PLGA core is labelled by encapsulation of 111In-doped iron oxide NPs inside PLGA-NPs during NP preparation, while the BSA coating is labelled by electrophilic substitution using 125I. After intravenous administration into rats, energy-discriminant single-photon emission computerised tomography (SPECT) resolved each radioisotope independently. Imaging revealed different fates for the core and coating, with a fraction of the two radionuclides co-localising in the liver and lungs for long periods of time after administration, suggesting that NPs are stable in these organs. The general methodology reported here represents an excellent alternative for visualising the degradation process of multi-labelled NPs in vivo and can be extended to a wide range of engineered NPs.

Imaging drug action in schizophrenia: research update

2003 ◽  
Vol 18 (S2) ◽  
pp. 32s-37s ◽  
Author(s):  
Marc Laruelle ◽  
Anissa Abi-Dargham
Keyword(s):  
Functional Neuroimaging ◽  
Single Photon ◽  
Photon Emission ◽  
Drug Action ◽  
Emission Tomography ◽  
Single Photon Emission ◽  
Computerised Tomography ◽  
Positron Emission ◽  
And Function

Functional neuroimaging using techniques such as positron emission tomography (PET) and single photon emission computerised tomography (SPECT) provide a direct in vivo assessment of the expression and function of neuroreceptors, transporters and enzymes. This article examines the technical aspects of molecular imaging and the application of those techniques in drug development.

A Pilot Study of Schizophrenia-Like Psychosis in Epilepsy Using Single-Photon Emission Computerised Tomography

1993 ◽  
Vol 163 (1) ◽  
pp. 32-36 ◽  
Author(s):  
E. Jane Marshall ◽  
G. M. S. Syed ◽  
P. B. C. Fenwick ◽  
W. A. Lishman
Keyword(s):  
Pilot Study ◽  
Single Photon ◽  
Photon Emission ◽  
Temporal Region ◽  
Single Photon Emission ◽  
Computerised Tomography ◽  
Matched Group

In a pilot study, SPECT was used to explore differences in rCBF between a group of patients with schizophrenia-like psychoses of epilepsy (SLPE) and a matched group of epileptic controls. Five patients in each group were investigated and those with SLPE showed significant reductions in the index of rCBF in the left medial temporal region. These differences are being investigated further.

Single Photon Emission Computerised Tomography in Chronic Alcoholism

1996 ◽  
Vol 169 (3) ◽  
pp. 348-354 ◽  
Author(s):  
Asli Çepik Kuruoglu ◽  
Zehra Arikan ◽  
Gülin Vural ◽  
Metin Karataş ◽  
Mehmet Araç ◽  
...  
Keyword(s):  
Auditory Evoked Potentials ◽  
Single Photon ◽  
Photon Emission ◽  
Daily Intake ◽  
Chronic Alcoholism ◽  
Low Flow ◽  
Single Photon Emission ◽  
Computerised Tomography ◽  
Drinking Habits ◽  
Personality Features

BackgroundWe examined the functional and structural cerebral changes in chronic alcoholics, analysing their association with personality features and alcohol drinking habits.MethodForty patients with alcohol dependency, including 15 with antisocial personality disorder (ASP) as defined in DSM–III–R and 10 age and sex matched healthy controls were studied after termination of withdrawal symptoms, using high resolution single photon emission tomography (SPECT), cranial computerised tomography (CT) and brainstem auditory evoked potentials (BAEP).ResultsWe found significant reductions in regional cerebral blood flow (rCBF) measurements of alcoholic patients. Low flow in frontal regions encountered in 67.5% of the patients was associated with the duration of alcohol consumption, while no such relation existed with the amount of daily intake. Patients with ASP exhibited more marked frontal hypoperfusion. Significant brain atrophy detected by CT was present in 40% of the patients and did not correlate with frontal hypoperfusion.ConclusionsPatients with ASP are more sensitive to toxic effects of alcohol. Alternatively chronic alcoholism leads to frontal lobe dysfunction recognised as ASP in the clinical setting.

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