The mobile phase motion in ascending Micellar thin-layer chromatography with normal-phase plates

2011 ◽  
Vol 24 (6) ◽  
pp. 463-469 ◽  
Author(s):  
Alexander Boichenko ◽  
Iryna Makhno ◽  
Anton Renkevich ◽  
Lidia Loginova
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Normal Phase ◽  
Phase Plates ◽  
Layer Chromatography ◽  
Phase Motion

scholarly journals Normal phase thin layer chromatography for aromatic derivatives of 3-chloro-1,4-naphtochinone

2021 ◽  
Vol 4 (1) ◽  
pp. 33-43
Author(s):  
O. Ya. Smirnova ◽  
◽  
Yo. Yo. Yatchyshyn ◽  
S. V. Kolobych ◽  
I. P. Poliuzhyn ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Normal Phase ◽  
Polar Component ◽  
Polar Solvents ◽  
Analyte Molecule ◽  
Binary Mobile Phase ◽  
Layer Chromatography ◽  
Derivatives Of

The chromatographic characteristics were investigated for eight aromatic derivatives of 3-chloro-1,4-naphthoquinone under conditions of normal-phase thin-layer chromatography for benzene-based binary mobile phase and such polar solvents as chloroform, acetone, acetonitrile, methanol and propan-2-ol. The slope of linear retention dependencies for the investigated compounds on the concentration of the polar component in the mobile phase satisfactorily correlates with the area occupied by the adsorbed analyte molecule in the stationary phase. The intercept in the Soczewinski equation depends on the polar component of the mobile phase.

Thin-layer chromatography of aflatoxins and zearalenones with β-cyclodextrins as mobile phase additives

2011 ◽  
Vol 4 (2) ◽  
pp. 113-117 ◽  
Author(s):  
D. Larionova ◽  
I. Goryacheva ◽  
C. Van Peteghem ◽  
S. De Saeger
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Limit Of Quantification ◽  
Mobile Phase Additives ◽  
Phase Plates ◽  
Aflatoxin B ◽  
Layer Chromatography ◽  
Quantitative Fluorescence

The conditions of thin-layer chromatography separation of related aflatoxins and zearalenones in the presence of β-cyclodextrin and 2-hydroxypropyl-β-cyclodextrin were studied. Effects of the stationary phase and mobile phase composition were investigated. Analytical conditions for the separation and simultaneous semi-quantitative fluorescence detection of aflatoxins B1, B2, G1 and G2, zearalenone and α-zearalenol on normal-phase plates (silica gel, polyamide) and reversed-phase plates (C18) with cyclodextrin modified mobile phase were optimised. The limit of quantification was found 2 ng per spot for aflatoxin G1 and aflatoxin B2, 3.5 ng for aflatoxin B1 and aflatoxin G2 and 100 ng per spot for zearalenone and α-zearalenol. Addition of cyclodextrins to the mobile phase allowed a decrease in the amount of toxic solvents, and improved separation characteristics, but did not improve the limit of quantification.

Thin-layer chromatography of laser dyes and dye analogs with cyclodextrins in the mobile phase

Talanta ◽  
1992 ◽  
Vol 39 (8) ◽  
pp. 953-958 ◽  
Author(s):  
I POLITZER ◽  
K CRAGO ◽  
K AMOS ◽  
K MITCHELL ◽  
T HOLLIN
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Laser Dyes ◽  
Layer Chromatography

scholarly journals Normal-phase thin-layer chromatography of some angiotensin converting enzyme (ACE) inhibitors and their metabolites

2009 ◽  
Vol 74 (6) ◽  
pp. 677-688 ◽  
Author(s):  
Jadranka Odovic ◽  
Mirjana Aleksic ◽  
Biljana Stojimirovic ◽  
Dusanka Milojkovic-Opsenica ◽  
Zivoslav Tesic
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ace Inhibitors ◽  
Reversed Phase ◽  
Normal Phase ◽  
Converting Enzyme ◽  
Reversed Phase Chromatography ◽  
Aqueous Solvent ◽  
Layer Chromatography

The separation and chromatographic behaviour of five ACE (angiotensin converting enzyme) inhibitors and their four active metabolites were investigated by normal-phase thin-layer chromatography on silica using several mono- and binary non-aqueous solvent systems. The linear relationship between the RM values and the composition of employed mobile phase was obtained. The hydrophobicity parameters 0M R and C0 were determined from the regression data of the plots, analogous to reversed-phase chromatography. The chromatographically obtained hydrophobicity parameters were correlated with the calculated log P values. The current results were correlated with the lipophilicity of the studied ACE inhibitors and their metabolites, previously estimated by reversed-phase chromatography.

scholarly journals Senyawa Saponin Hasil Isolasi dari Daun Buni (Antidesma bunius (L.) Spreng.)

2018 ◽  
Vol 1 (1) ◽  
pp. 264-270
Author(s):  
Hady Wiraputra ◽  
Marline Nainggolan ◽  
Panal Sitorus
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Preparative Thin Layer Chromatography ◽  
Ash Content ◽  
Hydroxyl Group ◽  
Chloroform Fraction ◽  
Aliphatic Group ◽  
Layer Chromatography

Tanaman buni (Antidesma bunius (L.) Spreng.) secara tradisional telah digunakan untuk hipertensi, takikardia, anemia, sifilis, antikanker, antioksidan, sumber pewarna alami dan antidiabetes. Saponin merupakan senyawa fitokimia yang mempunyai kemampuan membentuk busa dan mengandung aglikon polisiklik yang berikatan dengan satu atau lebih gula. Penelitian ini bertujuan untuk melakukan karakterisasi senyawa saponin hasil isolasi dari daun buni dengan spektrofotometer ultraviolet dan inframerah. Simplisia daun buni dilakukan karakterisasi kemudian diekstraksi dengan cara maserasi bertingkat menggunakan pelarut n-heksana dan etanol 80%. Selanjutnya ekstrak etanol dihidrolisis dengan HCl 2N kemudian difraksi dengan pelarut kloroform. Isolasi dilakukan terhadap fraksi kloroform dengan cara kromatografi lapis tipis preparatif menggunakan fase diam silika gel GF254 dan fase gerak yang sesuai. Isolat yang diperoleh diuji kemurnian dengan KLT 2 arah dan dikarakterisasi menggunakan spektrofotometer ultraviolet dan inframerah. Hasil pemeriksaan karakterisasi simplisia diperoleh kadar air 7,32%, kadar sari larut dalam etanol 52,70%, kadar sari larut dalam air 23,25%, kadar abu total 6,86% dan kadar abu tidak larut dalam asam 0,94%. Pemisahan fraksi kloroform dengan KLT menggunakan fase gerak n-heksana-etilasetat perbandingan 5:5 diperoleh noda 13 dan hasil KLT preparatif diperoleh 2 isolat murni yaitu isolat 1 (ungu merah) dengan Rf 0,92 dan isolat 2 (biru) dengan Rf 0,78. Hasil karakterisasi isolat 1 diperoleh panjang gelombang maksimum pada 208 nm dan dijumpai adanya gugus hidroksil, gugus -CH alifatis, ikatan C=C, gugus –CH2, gugus –CH3, dan gugus C-O. Hasil karakterisasi isolat 2 diperoleh panjang gelombang maksimum pada 204 nm dan adanya gugus hidroksil, gugus -CH alifatis, gugus –CH2, gugus –CH3, dan gugus C-O. Buni (Antidesmabunius (L.) Spreng.) has been traditionally used for the treatment of hypertension, tachycardia, anemia, syphilis, and used asanti-cancer, anti-oxidant, natural dye, and anti-diabetic. Saponin is a phytochemical compound which has capability in forming foam and contains polycyclic aglycone that binds with one or more glucose. This research aimed to conduct the characterization of saponin compound from buni leaves with ultraviolet spectrophotometer and infrared. Buni leaves simplicia was characterizedand extracted using sequential maceration method with n-hexane and 80% ethanol. The ethanol extract was hydrolyzed with HCl 2N and fractionized using chloroform solvent. Isolation of chloroform fraction was done using preparative thin-layer chromatography using silent phase of silica gel GF 254 and suitable mobile phase. Isolates obtained was taken into purity test with two dimensions thin-layer chromatography and characterized using ultraviolet spectrophotometer and infrared. The characterized simplicia resulted with 7.32% of water content, 52.70% of dissolved content in ethanol, 23.25% of dissolvedcontent in water, 6.86% of total ash content, and 0.94% of undissolved ash content in acid. Fractinationof chloroform fraction with thin-layer chromatography using mobile phase ofn-hexane-ethyl acetate with 5:5 ration resulted with 13 spotsand the result of the preparative thin-layer chromatography resulted 2 pure isolates which are isolate 1 (purple-red) with Rf 0.92 and isolate 2 (blue) with Rf 0.78. The characterization of isolate 1 resulted that the maximum wave lengthwas 208 nm with hydroxyl group, –CH aliphatic group, C=C bond, –CH2 group, –CH3 group, and C–O group. The characterization of isolate 2 resulted that the maximum wave lengthwas204 nm with hydroxyl group, –CH aliphatic group, –CH2 group, –CH3 group, and C–O group.

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