scholarly journals First detection of Pasteurella multocida type B:2 in Hungary associated with systemic pasteurellosis in backyard pigs

2015 ◽  
Vol 63 (2) ◽  
pp. 141-156 ◽  
Author(s):  
Barbara Ujvári ◽  
Levente Szeredi ◽  
László Pertl ◽  
Gergely Tóth ◽  
Károly Erdélyi ◽  
...  
Keyword(s):  
Small Area ◽  
Pasteurella Multocida ◽  
Sequence Type ◽  
Type B ◽  
Chain Reaction ◽  
Haemorrhagic Septicaemia ◽  
Gene Typing ◽  
Polymerase Chain ◽  
Serotype B ◽  
Backyard Pigs

This is the first report of Pasteurella multocida type B in Hungarian pigs. This disease was observed in backyard-raised pigs in three households within a small area. Neither the source of the infection nor the epidemiological connection between any of the premises could be determined. The most consistent lesion was dark red discolouration of the skin of the ventral neck and brisket, with accompanying oedema and haemorrhages. The morbidity was low and lethality relatively high, with three dead (50%) and two euthanised (33%) out of six affected animals. A total of three isolates of P. multocida (P55, P56 and P57) were cultured from these cases and examined in detail. These were identified as P. multocida ssp. multocida biovar 3. All were toxA negative and belonged to serotype B:2. Multilocus sequence typing was used to assign these to a new sequence type (ST61) that is closely related to other haemorrhagic septicaemia causing strains of P. multocida regardless of the host. M13 polymerase chain reaction and virulence-associated gene typing also show that type B strains form a highly homogeneous, distinct phylogenic group within P. multocida.

scholarly journals Molecular identification by multiplex polymerase chain reaction of Pasteurella multocida in cattle and buffaloes in Baghdad

2014 ◽  
Vol 38 (1) ◽  
pp. 99-106
Author(s):  
Ihab G. M. AL-Shemmari
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Pcr ◽  
Multiplex Polymerase Chain Reaction ◽  
Pasteurella Multocida ◽  
Type B ◽  
Chain Reaction ◽  
Blood Samples ◽  
Nasal Swabs ◽  
Polymerase Chain ◽  
B 31

The aim of this study was to identify pasteurella multocida and their types by PCR in cattle’s and buffaloesi bagdad from March to August 2012 on 204 animals , including 102 cattle and 102 buffaloes at slaughter houses from Baghdad .Blood samples and nasal swaps were collected , before slaughtering and lung tissues of slaughtered animal , and from 54 clinically suspected cases of pasteurellosis , including 27 bovines ,and 27 buffaloes the samples taken included blood and nasal swabs . Pasteurellamultocida were isolated from 94 animals include 49 cattle 45 buffaloes. The typing of the isolates by multiplex PCR for genotyping Pasteuerllamultocida revealed 93 isolates of type B , 31 from cattle and 62 from buffaloes ,and 81 isolates of type A , 55 from cattle and 26 from buffaloes .

scholarly journals Polymerase Chain Reaction Detection of Pasteurella multocida Type B:2 in Mice Following Oral Inoculation

2013 ◽  
Vol 8 (3) ◽  
pp. 493-501 ◽  
Author(s):  
Faez Firdaus Je ◽  
Abdinasir Yusuf Osma ◽  
Lawan Adamu ◽  
Mohd Syamil Moh ◽  
Abdul Rahman Oma ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pasteurella Multocida ◽  
Type B ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Oral Inoculation ◽  
Polymerase Chain

scholarly journals Molecular detection of Pasteurella multocida Type B causing haemorrhagic septicemia in cattle and buffaloes of Bangladesh

2016 ◽  
Vol 27 (2) ◽  
pp. 175-179 ◽  
Author(s):  
MS Ara ◽  
MT Rahman ◽  
M Akhtar ◽  
M Rahman ◽  
KHMNH Nazir ◽  
...  
Keyword(s):  
Morphological Study ◽  
Pasteurella Multocida ◽  
Molecular Detection ◽  
Vaccine Candidate ◽  
Clinical Samples ◽  
Effective Vaccine ◽  
Type B ◽  
Chain Reaction ◽  
Specific Primers ◽  
Polymerase Chain

Hemorrhagic septicemia (HS) is an acute septicemic disease that primarily affects cattle and buffaloes. The disease is caused by Pasteurella multocida sero types B:2 and E:2. The objective of this study was to isolate P. multocida from clinical cases and to confirm its identity using polymerase chain reaction (PCR) based approach. Clinical samples of two suspected cases of haemorrhagic septicemia of cattle and buffalo from Mymensingh and Rajshahi districts respectively were collected. Two isolates were isolated from these suspected cases and primarily identified as P. multocida based on morphological study, staining properties, and cultural and biochemical characteristics. The isolates were confirmed initially as P. multocida at genus level by PCR using genus specific primers. Later, the isolates were confirmed as P. multocida type B, the causal agent of haemorrhagic septicemia, by PCR with primers specific for P. multocida type B. These isolated organisms can be used as vaccine candidate for the production of effective vaccine against haemorrhagic septicemia.Progressive Agriculture 27 (2): 175-179, 2016

scholarly journals Identification of Neisseria gonorrhoeae isolates with a recombinant porA gene in Scotland, United Kingdom, 2010 to 2011

2012 ◽  
Vol 17 (9) ◽  
Author(s):  
K Eastick ◽  
A Winter ◽  
S Jamdar
Keyword(s):  
Polymerase Chain Reaction ◽  
United Kingdom ◽  
Neisseria Gonorrhoeae ◽  
Sequence Type ◽  
The Other ◽  
Chain Reaction ◽  
Polymerase Chain

Three isolates of Neisseria gonorrhoeae have been identified in Scotland in 2010 and 2011, which lack sequences in the porA pseudogene commonly used as the target for confirmatory gonorrhoea polymerase chain reaction assays. Two isolates were clustered temporally and geographically and have the same sequence type and porA sequence. A similar strain was reported in Australia during early 2011. The other Scottish isolate was identified separately and is different in sequence type and porA sequence.

scholarly journals Mass spectrometric analysis of lipid A obtained from the lipopolysaccharide of Pasteurella multocida

RSC Advances ◽  
2020 ◽  
Vol 10 (51) ◽  
pp. 30917-30933
Author(s):  
Abdul Tawab ◽  
Noor Akbar ◽  
Mujtaba Hasssan ◽  
Fazale Habib ◽  
Aamir Ali ◽  
...  
Keyword(s):  
Causative Agent ◽  
Pasteurella Multocida ◽  
Lipid A ◽  
Water Buffalo ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Type B ◽  
Haemorrhagic Septicaemia

LC/MS-based variant profiling of lipid A component of endotoxic lipopolysaccharides of Pasteurella multocida type B:2, a causative agent of haemorrhagic septicaemia in water buffalo and cattle.

scholarly journals Precision of genotyping of Epstein-Barr virus by polymerase chain reaction using three gene loci (EBNA-2, EBNA-3C, and EBER): predominance of type A virus associated with Hodgkin's disease [published erratum appears in Blood 1993 Oct 1;82(7):2268]

Blood ◽  
1993 ◽  
Vol 81 (12) ◽  
pp. 3372-3381 ◽  
Author(s):  
JC Lin ◽  
SC Lin ◽  
BK De ◽  
WC Chan ◽  
BL Evatt ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cell Lines ◽  
Epstein Barr Virus ◽  
Point Mutations ◽  
Type A ◽  
Type B ◽  
Chain Reaction ◽  
Barr Virus ◽  
Polymerase Chain ◽  
Epstein Barr

Abstract To precisely determine the genotype of Epstein-Barr virus (EBV) in Hodgkin's disease (HD), we simultaneously analyzed three divergent gene loci (EBNA-2, EBNA-3C, and EBER) that distinguish type A and B viruses. The primers designed to amplify these three gene loci encompass either type-specific deletion sequences (EBNA-2 and EBNA-3C) or type-specific point mutations (EBER) that identify the virus strain based on the sizes of the polymerase chain reaction (PCR)-amplified products or the mobility shifts in single-strand conformation polymorphism analysis. The locations of point mutations were identified by direct sequencing of the PCR-amplified DNA. We analyzed 15 EBV-infected cell lines and found a good correlation between EBNA-2 and EBNA-3C typing results. In contrast, approximately 33% of the cell lines analyzed maintained type A sequences in EBNA-2 and EBNA-3C genes while carrying type B sequences in the EBER region. Data obtained from analysis of cell lines served as a reference for studying HD samples. EBV DNA was detected in about 70% of HD. Among the EBV-positive samples, 56% were associated with type A virus, 13% with type B, and 31% with dual viral sequences. Thus, type A virus is predominant in HD. Based on the histology, the frequencies of EBV positivity were 83%, 71%, and 33% for mixed cellularity, nodular sclerosis, and lymphocyte predominance, respectively. The detection of high frequency of both type A and B sequences in HD may provide a lead in investigating the role of dual viral infection in EBV pathogenesis.

Phenotypic and genotypic analysis of Flavobacterium psychrophilum isolates from Ontario salmonids with bacterial coldwater disease

2008 ◽  
Vol 54 (8) ◽  
pp. 619-629 ◽  
Author(s):  
Shohreh Hesami ◽  
Katie J. Allen ◽  
Devon Metcalf ◽  
Vaughn E. Ostland ◽  
Janet I. MacInnes ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Rainbow Trout ◽  
16S Rrna ◽  
Sequence Type ◽  
Chain Reaction ◽  
Flavobacterium Psychrophilum ◽  
Genotypic Analysis ◽  
Polymerase Chain ◽  
Coldwater Disease ◽  
Bacterial Coldwater Disease

Flavobacterium psychrophilum is the causative agent of bacterial coldwater disease (BCWD) and rainbow trout fry syndrome. BCWD has a considerable economic impact on aquaculture operations in Ontario, Canada, and our limited understanding of the population structure and epidemiology of F. psychrophilum isolates is an impediment to the development of improved management strategies. Seventy-five 16S rRNA gene and gyr polymerase chain reaction positive isolates of F. psychrophilum that had been collected over a 16-year period from farmed salmonids with tail rot, necrotic myositis, and osteochondrosis were characterized morphologically, biochemically, and genotypically. Although the isolates were homogeneous by preliminary biochemical and phenotypic characterization, two distinct biovars were found by API ZYM testing. As well, four restriction pattern types were detected by 16S rRNA polymerase chain reaction – restriction fragment length polymorphism analysis and there was a significant (P < 0.001) correlation between biovar I and digestion with MaeIII and between biovar II and digestion with MnlI or no site (P < 0.05). Further heterogenity was detected by sequence analysis of a 194 bp stem loop 3 region of rRNA. Nine sequence types were identified; 40/46 biovar I isolates were sequence type “a”, while 21/32 biovar II isolates belonged to either sequence type “c” or “d”. More than one biovar and genotype was identified among the strains recovered from separate fish sampled from three groups of rainbow trout ( Oncorhynchus mykiss ) experiencing BCWD mortality events. No association was found between genotype or biovar and type of disease. Taken together, these data suggest that F. psychrophilum from Ontario can be grouped into two major lineages based on biovar and 16S rRNA polymorphisms, and although three major strain types were most frequently isolated in this study, it appears that the population of F. psychrophilum with pathogenic potential is quite heterogeneous.

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