scholarly journals A szájüreg baktériumflórájának összefüggése az anastomosiselégtelenség következményeivel nyelőcső- és cardiatumoros betegeknél

2017 ◽  
Vol 158 (1) ◽  
pp. 25-30
Author(s):  
Ákos Balázs ◽  
Beáta Winkler ◽  
Katalin Kristóf ◽  
László Harsányi ◽  
Lívia Bokor
Keyword(s):  
Oral Cavity ◽  
Anastomotic Leak ◽  
Pathogenic Bacteria ◽  
Current Practice ◽  
Surgical Intervention ◽  
Bacterial Flora ◽  
Cavity Method ◽  
Moderate Correlation ◽  
Anastomotic Insufficiency ◽  
Oral Microflora

Abstract: Introduction: In the course of anastomotic insufficiency following resection of esophageal cancers the bacterial compound of the esophageal substance has a remarkable, presumable role in the outcome of complications. Aim: The purpose of this study is to compare the consequences of the anastomotic leak with the bacterial flora of patients’ oral cavity. Method: In this prospective study a total of 131 patients were investigated directly before the surgical intervention taking a bacterial sample. Bacterial flora of patients’ oral cavity was analysed; and the correlation between the consequences of the anastomotic leak and the content of the bacterial flora was examined. Results: Pathogenic bacteria in the oral microflora in 50 cases (38.2%) was found. Statistically significant, moderate correlation was found between the severity of the complication and the incidence of pathogenic bacteria (rs = 0.553; p≤0.05). Conclusions: Pathogenic agent in the microbial flora might induce higher risk and more severe outcome in case of anastomotic leakage and it might be evaluated as a determinative factor. Consideration of the bacterial flora of the oral cavity requires more attention in the preoperative preparation than before and it demands the change of the current practice. Orv. Hetil., 2017, 158(1), 25–30.

Bacteriology of the oral cavity of BALB/c mice

1986 ◽  
Vol 32 (8) ◽  
pp. 673-678 ◽  
Author(s):  
L. Trudel ◽  
L. St-Amand ◽  
M. Bareil ◽  
P. Cardinal ◽  
M. C. Lavoie
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Oral Cavity ◽  
Bacterial Flora ◽  
The Other ◽  
Limited Data ◽  
Oral Flora ◽  
Predominant Species ◽  
Oral Microflora ◽  
Lactobacillus Murinus

To be used as a model in dental research, an animal must fulfil experimental needs and information on the composition and variation of its oral flora must be available. Only limited data are available on the indigenous oral bacterial flora of BALB/c mice. In this work, a total of 671 isolates from different sites (saliva, tongue, teeth, and mucosa) of the oral cavity of BALB/c mice were identified. Only 18 different species were isolated, which indicates the relative simplicity of the flora. The predominant species of the total cultivable flora were "Lactobacillus muritius" (38%), Staphylococcus aureus (37%), Streptococcus faecalis (8%), Staphylococcus sciuri (4%), and Escherichia coli (3%). The other species each represent less than 2% of the flora. "Lactobacillus murinus" is found in greater proportion on mucosa than in the other sites, Staph. aureus predominates in saliva, and Strep. faecalis was found in greater proportion in tooth samples. Statistical analyses, using the minimum percentage of similarity, indicate that there is some variation among the microflora of different mice but that this difference is smaller for mice from the same lot. These results set the basis for the study of the variations of the indigenous oral microflora of BALB/c mice under different conditions.

scholarly journals Dysbiosis of Oral Microflora: A Review

2018 ◽  
Vol 08 (04) ◽  
pp. 034-039
Author(s):  
Mithra N Hegde ◽  
Keyword(s):  
Oral Cavity ◽  
Pathogenic Bacteria ◽  
Human Microbiome ◽  
Health Benefit ◽  
The Body ◽  
Oral Disease ◽  
Oral Microflora ◽  
Environmental Properties ◽  
Metabolic Activities ◽  
Varying Environment

AbstractMicroorganisms being an integral part of human body colonise various sites, with oral cavity being one of the most densely populated environment. Within the oral cavity there are varying environment, properties of which determines the type of microbes colonising the site, while the metabolic activities of these microorganisms later on modifies the environmental properties. These microorganisms when in equilibrium confer health benefit however any alteration in the flora allows the pathogenic bacteria to outgrow in numbers and cause oral disease. Such alteration could be due to various factors. The present review article focuses on the various aspects of oral microbial flora, their role in the body, dysbiosis and factors influencing along with the reestablishment of normal healthy microbiome. A search was made on pubmed and scopus using keywords and 25 relevant articles published during 2000 to 2018 along with their references were included in the study.With increasing knowledge of human microbiome, attempts are made to limit the alteration in oral ecosystem or re-establish the normal healthy flora as a part of prevention or treatment of diseases. This brings about change in approach which initially focused on elimination of microbes to maintaining their equilibrium.

scholarly journals Impact of the Diet on the Formation of Oxidative Stress and Inflammation Induced by Bacterial Biofilm in the Oral Cavity

Materials ◽  
2021 ◽  
Vol 14 (6) ◽  
pp. 1372
Author(s):  
Ilona Rowińska ◽  
Adrianna Szyperska-Ślaska ◽  
Piotr Zariczny ◽  
Robert Pasławski ◽  
Karol Kramkowski ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oral Cavity ◽  
Pathogenic Bacteria ◽  
Soft Tissues ◽  
Bacterial Flora ◽  
Bacterial Biofilm ◽  
Oral Microbiome ◽  
Diffusion Method ◽  
Oral Diseases ◽  
Periodontal Tissues

The diet is related to the diversity of bacteria in the oral cavity, and the less diverse microbiota of the oral cavity may favor the growth of pathogenic bacteria of all bacterial complexes. Literature data indicate that disturbances in the balance of the bacterial flora of the oral cavity seem to contribute to both oral diseases, including periodontitis, and systemic diseases. If left untreated, periodontitis can damage the gums and alveolar bones. Improper modern eating habits have an impact on the oral microbiome and the gut microbiome, which increase the risk of several chronic diseases, including inflammatory bowel disease, obesity, type 2 diabetes, cardiovascular disease and cancer. The subject of our consideration is the influence of the traditional diet on the formation of oxidative stress and inflammation caused by bacterial biofilm in the oral cavity. Through dental, biomedical and laboratory studies, we wanted to investigate the effect of individual nutrients contained in specific diets on the induction of oxidative stress inducing inflammation of the soft tissues in the oral cavity in the presence of residual supra- and subgingival biofilm. In our research we used different types of diets marked as W, T, B, F and noninvasively collected biological material in the form of bacterial inoculum from volunteers. The analyzed material was grown on complete and selective media against specific strains of all bacterial complexes. Additionally, the zones of growth inhibition were analyzed based on the disc diffusion method. The research was supplemented with dental and periodontological indicators. The research was supplemented by the application of molecular biology methods related to bacterial DNA isolation, PCR reactions and sequencing. Such selected methods constitute an ideal screening test for the analysis of oral bacterial microbiota. The obtained results suggest that certain types of diet can be an effective prophylaxis in the treatment of civilization diseases such as inflammation of the oral cavity along with periodontal tissues and gingival pockets.

Reliability of Hammersmith Examination in Prediction of Neurological Outcomes (REHAPENO) - Research Protocol (Preprint)

2018 ◽  
Author(s):  
Mohamed Fleifel ◽  
Rawya Abdelghani ◽  
Mohamed Ameen
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Image ◽  
Current Practice ◽  
Surgical Intervention ◽  
Brain Mri ◽  
Developmental Outcomes ◽  
Research Protocol ◽  
Neurological Outcomes ◽  
Mri Brain ◽  
Brain Ultrasound

BACKGROUND Background: Studying the neurological developmental outcomes and comparing correlations with MRI (Magnetic resonance image) versus the Hammersmith Infant Neurological Examination (HINE) OBJECTIVE Objective: To investigate the non-inferiority of MRI to HINE in infant developmental outcomes METHODS Settings: Hospital settings including pediatrics and neonatal care units Intervention: No medical or surgical intervention is planned, only correlation and extra analyses would take place to standardize the current practice Measurements: HINE, Brain MRI, Brain Ultrasound and developmental outcomes after 12 months RESULTS Results: The observations collected and correlations measured to figure out the reliability of both HINE and MRI in order to figure to what extent can we rely on HINE alone in expecting the developmental outcomes CONCLUSIONS The more reliability would expressed by HINE assessment the accurate expectation of developmental in preterm infants CLINICALTRIAL https://clinicaltrials.gov/ct2/show/NCT03580252

EXPRESIÓN DE P53 EN CULTIVO DE CÉLULAS MADRE AISLADAS A PARTIR DE QUISTE RADICULAR INFLAMATORIO

SCIENTIARVM ◽  
2015 ◽  
Vol 1 (1) ◽  
pp. 33-36
Author(s):  
Francis Wendell Jácobo Valdivia ◽  
◽  
Julio Cesar Bernabé Ortiz ◽  
Javier Valero Quispe ◽  
Ivo Palomino Valverde ◽  
...  
Keyword(s):  
Stem Cells ◽  
Oral Cavity ◽  
Surgical Intervention ◽  
Positive Response ◽  
P53 Gene ◽  
Cell Cytoplasm ◽  
Inflammatory Lesions

The inflammatory root cyst is defined as one of the most frequent inflammatory lesions in the oral cavity where it normally involves the apex of the dental roots, giving frequent pathognomonic clinical and radiological characteristics. The objective of this investigation was to determine the expression of p53 in the culture of isolated stem cells from the inflammatory root cyst. In its methodology, the report of a case of a 69-year-old patient was presented, who underwent surgical intervention to extract pieces 11 and 12, from which the sample was obtained for the isolation and culture of the cyst stem cells. periapical inflammatory, obtaining elongated, flat cells with fibroblastic appearance from day 4 and staining with markers for p53 giving a positive response in the evidence of the p53 gene both in the cytoplasm and in the nucleus of these stem cells. Finally, cells can be obtained from the inflammatory root cyst from 4 to 25 days with the use of a modified protocol, in the end, as a contribution to this article, the evidence of the p53 gene is provided both in the nucleus and in the cell cytoplasm. Keywords: Root cyst, p53 gene, stem cells, isolation and culture

RA05.04: MANAGEMENT & OUTCOMES OF ANASTOMOTIC LEAKS FOLLOWING ESOPHAGECTOMY: A PROSPECTIVE 10-YEAR SINGLE-SITE EXPERIENCE

2018 ◽  
Vol 31 (Supplement_1) ◽  
pp. 28-28
Author(s):  
Joel Lambert ◽  
Sanya Caratella ◽  
Eloise Lawrence ◽  
Bilal Alkhaffaf
Keyword(s):  
Minimally Invasive ◽  
Anastomotic Leak ◽  
Surgical Intervention ◽  
Type I ◽  
Oral Contrast ◽  
Open Approach ◽  
Type Ii ◽  
Anastomotic Leaks ◽  
Type Iii ◽  
Significant Difference

Abstract Background Anastomotic leak after esophagectomy is associated with high levels of morbidity and may impact negatively on oncological outcomes. The aim of this single centre study was to describe our experience in managing these complications Methods From 2007–2017 data was reviewed retrospectively from our prospectively maintained electronic database. All patients underwent either 2 or 3 phase esophagectomy for cancer of the oesophagus or esophago-gastric junction. All histological sub-types and stage of cancer were included in the analysis. Anastomotic leaks were classified according to the Esophagectomy Complications Consensus Group (ECCG) guidelines; type I—conservative management, type II—non-surgical intervention, type III—surgical intervention. Results 224 esophagectomies were included in our analysis (104 (46%) minimally invasive, 120 (54%) open approach). The incidence of all anastomotic leaks was 10% (23/224). Surgical approach did not influence the incidence of anastomotic leak (minimally invasive 10 (43%), open approach 13(57%), P = 0.76). Five patients (22%) had a type I leak, 9 patients (39%) type II and 9 (39%) had a type III leak. There was an increase in the number of leaks managed non-surgically over the last 5 years compared to those in the first five years of our dataset (2012–2017: 11/23 (48%) vs 2007–2012: 4/23 (17%) P = 0.08). The median time for leak diagnosis was 8 days. Most leaks were diagnosed with oral contrast CT 19 (83%). Median hospital stay after anastomotic leak was 58.5 days. Type III leaks were associated with an increased length of stay (median 84 days) compared to type I&II leaks (median (38.5 days) (P = 0.002 95% CI 18.19- 74.41). There was no significant difference in 30-day mortality between type I&II (0 patients) and type III leaks (1 patient) P = 0.260. Conclusion Low mortality rates with anastomotic leak can be achieved. In centres with experienced radiological and endoscopic skills, most anastomotic leaks can be managed non-surgically. Disclosure All authors have declared no conflicts of interest.

Aerobic and Anaerobic Bacterial Flora of the Maternal Cervix and Newborn Gastric Fluid and Conjunctiva: A Prospective Study

PEDIATRICS ◽  
1979 ◽  
Vol 63 (3) ◽  
pp. 451-455
Author(s):  
Itzhak Brook ◽  
Cynthia T. Barrett ◽  
Charles R. Brinkman ◽  
William J. Martin ◽  
Sydney M. Finegold
Keyword(s):  
Pathogenic Bacteria ◽  
Bacterial Flora ◽  
Newborn Infants ◽  
Gastric Fluid ◽  
Viridans Streptococci ◽  
Prolonged Duration ◽  
Bacteroides Fragilis Group ◽  
A Prospective Study ◽  
Aerobic And Anaerobic ◽  
Duration Of Pregnancy

Aerobic and anaerobic cultures were performed on cervical swabs from 35 mothers and their newborn infants' gastric aspirates and conjunctival sacs. Four hundred seventy-nine isolates were obtained; 287 were aerobes and 192 were anaerobes. The cervical and gastric cultures overall yielded a similar flora, but this was not always the case when one compared mothers with their own newborn infants. The conjunctival cultures yielded about half of the number of bacteria per specimen that the gastric contents yielded, and a repeat conjunctival culture done 48 hours later showed a marked reduction in the number of potentially pathogenic bacteria isolated. The predominant aerobes isolated were Staphylococcus epidermidis, diphtheroids, viridans streptococci and Haemophilus vaginalis. The predominant anaerohes were the Bacteroides fragilis group, Propionihacterium acnes, Peptococcus, other Bacteroides and Peptostreptococcus organisms. Statistical analysis revealed significant positive correlation between the isolation of certain groups of organisms and increased duration of pregnancy, increased baby's weight, and prolonged duration of labor.

Evaluation of cultivable aerobic bacterial flora from Russell's viper (Daboia russelii) oral cavity

2019 ◽  
Vol 134 ◽  
pp. 103573 ◽  
Author(s):  
Sujogya Kumar Panda ◽  
Laxmipriya Padhi ◽  
Gunanidhi Sahoo
Keyword(s):  
Oral Cavity ◽  
Bacterial Flora ◽  
Russell’S Viper

Antimicrobial Resistance of Bacterial Flora Associated with Bovine Products in South Africa

1999 ◽  
Vol 62 (6) ◽  
pp. 615-618 ◽  
Author(s):  
THUREYAH MANIE ◽  
VOLKER S. BRÖZEL ◽  
WALTER J. VEITH ◽  
PIETER A. GOUWS
Keyword(s):  
South Africa ◽  
Antibiotic Resistance ◽  
Pathogenic Bacteria ◽  
Bacterial Flora ◽  
Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Selective Pressures ◽  
Unpasteurized Milk ◽  
Plate Counts ◽  
Resistance To Penicillin

The administration of subtherapeutic doses of antibiotics to livestock introduces selective pressures that may lead to the emergence and dissemination of resistant bacteria. This study determined the antibiotic-resistance spectra of the microbial flora found on freshly slaughtered and retail beef and in unpasteurized and pasteurized packaged milk. Staphylococci, Enterobacteriaceae, and isolates from total aerobic plate counts were tested for resistance to vancomycin, streptomycin, methicillin, tetracycline, and gentamicin using the disc diffusion susceptibility test and resistance to penicillin was determined by using oxacillin. A larger proportion of resistance to most antibiotics, except for vancomycin, was displayed by isolates from abattoir samples. The incidence of multiple antibiotic resistance (MAR) pathogenic bacteria is also higher in the abattoir. Resistance genes lost because of lack of selective pressure or resistant flora being replaced by more sensitive flora during processing is the reason for the lower incidence of MAR pathogenic bacteria among retail samples. These resistant bacteria can be transferred to humans through the consumption of rare or raw beef and unpasteurized milk, thus rendering the resultant food-related infections difficult to treat. The present findings clearly demonstrate that antibiotic-resistant bacteria in beef and milk pose a serious problem in South Africa.

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