EXPRESIÓN DE P53 EN CULTIVO DE CÉLULAS MADRE AISLADAS A PARTIR DE QUISTE RADICULAR INFLAMATORIO

SCIENTIARVM ◽  
2015 ◽  
Vol 1 (1) ◽  
pp. 33-36
Author(s):  
Francis Wendell Jácobo Valdivia ◽  
◽  
Julio Cesar Bernabé Ortiz ◽  
Javier Valero Quispe ◽  
Ivo Palomino Valverde ◽  
...  
Keyword(s):  
Stem Cells ◽  
Oral Cavity ◽  
Surgical Intervention ◽  
Positive Response ◽  
P53 Gene ◽  
Cell Cytoplasm ◽  
Inflammatory Lesions

The inflammatory root cyst is defined as one of the most frequent inflammatory lesions in the oral cavity where it normally involves the apex of the dental roots, giving frequent pathognomonic clinical and radiological characteristics. The objective of this investigation was to determine the expression of p53 in the culture of isolated stem cells from the inflammatory root cyst. In its methodology, the report of a case of a 69-year-old patient was presented, who underwent surgical intervention to extract pieces 11 and 12, from which the sample was obtained for the isolation and culture of the cyst stem cells. periapical inflammatory, obtaining elongated, flat cells with fibroblastic appearance from day 4 and staining with markers for p53 giving a positive response in the evidence of the p53 gene both in the cytoplasm and in the nucleus of these stem cells. Finally, cells can be obtained from the inflammatory root cyst from 4 to 25 days with the use of a modified protocol, in the end, as a contribution to this article, the evidence of the p53 gene is provided both in the nucleus and in the cell cytoplasm. Keywords: Root cyst, p53 gene, stem cells, isolation and culture

scholarly journals A Review of the Pathogenesis of Osteoarthritis and the Use of Intra-articular Platelet Therapy for Joint Disease in Animals and Humans

2014 ◽  
Vol 5 ◽  
pp. BTRI.S14578 ◽  
Author(s):  
Girolamo A. Ortolano ◽  
Barry Wenz
Keyword(s):  
Health Care ◽  
Stem Cells ◽  
Surgical Intervention ◽  
Domestic Animals ◽  
Medical Intervention ◽  
Joint Disease ◽  
Therapeutic Approaches ◽  
Disease Modifying ◽  
Care Costs ◽  
Clinical Problems

Osteoarthritis (OA) is the most prevalent musculoskeletal disease in humans and domestic animals. It causes significant clinical problems and substantial health care costs. In the absence of disease-modifying medical intervention, therapy is currently restricted to palliative measures prior to surgical intervention. We review the pathogenesis, as well as conservative and emerging restorative therapeutic approaches, including cytokines, stem cells, and platelets. The various methods of platelet concentrate preparations and their reported outcomes are discussed. Data collected from the use of intra-articular platelet therapy (IAPT) in dogs are reviewed, which suggest that this approach may delay or in some cases even obviate the need for surgical intervention.

Dormancy activation mechanism of oral cavity cancer stem cells

Tumor Biology ◽  
2015 ◽  
Vol 36 (7) ◽  
pp. 5551-5559 ◽  
Author(s):  
Xiang Chen ◽  
Xin Li ◽  
Baohong Zhao ◽  
Dehao Shang ◽  
Ming Zhong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Oral Cavity ◽  
Oral Cavity Cancer ◽  
Activation Mechanism

Speech and Resonance Disorders Related to Cleft Palate and Velopharyngeal Dysfunction: A Guide to Evaluation and Treatment

2014 ◽  
Vol 15 (2) ◽  
pp. 57-74 ◽  
Author(s):  
Ann W. Kummer
Keyword(s):  
Oral Cavity ◽  
Cleft Palate ◽  
Speech Therapy ◽  
Surgical Intervention ◽  
Speech Language Pathologists ◽  
Practical Information ◽  
Nasal Emission ◽  
Structural Anomalies

Children with speech and/or resonance disorders due to structural anomalies present challenges for speech-language pathologists in all settings. The purpose of this article is to provide current, practical information regarding the evaluation and treatment of children with speech/resonance disorders secondary to cleft palate and noncleft causes of velopharyngeal dysfunction. Simple “no-tech” procedures for assessment will be described so that the reader will be able to determine which children will benefit from therapy and which will require surgical intervention. Finally, specific speech therapy techniques will be given for changing placement from the pharynx to the oral cavity, thus eliminating phoneme-specific nasal emission.

Stem Cells in the Oral Cavity

2017 ◽  
Vol 8 (3) ◽  
pp. 99-103
Author(s):  
Chadi Torbay ◽  
Fadl Khaled
Keyword(s):  
Stem Cells ◽  
Oral Cavity

scholarly journals Basal stem cells contribute to squamous cell carcinomas in the oral cavity

2013 ◽  
Vol 34 (5) ◽  
pp. 1158-1164 ◽  
Author(s):  
Xiao-Han Tang ◽  
Theresa Scognamiglio ◽  
Lorraine J. Gudas
Keyword(s):  
Stem Cells ◽  
Oral Cavity ◽  
Squamous Cell ◽  
Squamous Cell Carcinomas

scholarly journals The oral cavity – potential source of stem cells

2017 ◽  
Vol 71 (0) ◽  
pp. 0-0 ◽  
Author(s):  
Rafał Brożek ◽  
Maciej Kurpisz ◽  
Ryszard Koczorowski
Keyword(s):  
Stem Cells ◽  
Oral Cavity ◽  
Current Knowledge ◽  
Easy Access ◽  
Moral Issues ◽  
Stomatognathic System ◽  
Dental Origin ◽  
The Common ◽  
And Function ◽  
Real Chance

The purpose of this review is to present the current knowledge regarding the hierarchy of stem cells originating from the oral cavity, which could have a potential value when applied to regenerative stomatology. It must be particularly emphasized that the heterogenous nature of its biology and function within oral compartment may predispose them to different types of applications. Stem cells can be perceived as immature, primitive and unspecialized types of cells with the ability to proliferate, self-renew and differentiate into specialized progeny according to the compartmental signaling. Their presence in tissue reservoirs was already discovered in many organs and tissues as well as in the stomatognathic system. The oral cavity appears to be an exceptionally attractive site to acquire stem cells. The common presence and easy access to these cells in dental and peridental tissues provides a real chance to apply them for therapeutic purposes. Such an opportunity would also be neutral to bioethical and moral issues, assuming autologous stem cells employment. Many authors suspect that stem cells have epigenetic memory, so some of their features can be inherited through generations. They are not connected, however, with DNA sequence modifications. It is, therefore, justified to apply the cells, which have the oral cavity as their natural reservoir, in interventions associated with tissue engineering within the stomatognathic system. An increasing number of clinical trials, among which the number of randomized studies with large group of patients is progressively carried out, allows for a prediction that shortly therapeutic methods based on stem cells of dental origin may be implemented to the routine repertoire of clinical practice.

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