scholarly journals Seroprevalence of Anaplasma phagocytophilum, Ehrlichia spp. and Borrelia burgdorferi infections in horses: first report from Northern Bulgaria – Short communication

2019 ◽  
Vol 67 (2) ◽  
pp. 197-203 ◽  
Author(s):  
Ilia Tsachev ◽  
Magdalena Baymakova ◽  
Nikola Pantchev
Keyword(s):  
Borrelia Burgdorferi ◽  
Anaplasma Phagocytophilum ◽  
Short Communication ◽  
Elisa Test ◽  
Limited Information ◽  
North Central ◽  
Mean Frequency ◽  
First Report ◽  
Granulocytic Anaplasmosis ◽  
Natural Exposure

Lyme borreliosis, granulocytic anaplasmosis and monocytic ehrlichiosis are well studied in humans and dogs. In horses, these diseases are not widely investigated and limited information is available about their occurrence. The purpose of this study was to present the first ELISA-based report on the seroprevalence ofAnaplasma phagocytophilum, Ehrlichiaspp. andBorrelia burgdorferiin horses from Northern Bulgaria. A total of 192 horses were investigated from three regions in Northern Bulgaria (Northwestern, North-Central and Northeastern Bulgaria). All equine sera were tested forA. phagocytophilum, Ehrlichiaspp. andB. burgdorferiantibodies by a commercial rapid ELISA test. Antibodies againstA. phagocytophilumwere found in all the three regions at a mean frequency of 12% (23/192), ranging from 9.38 to 15.63% by region. Antibodies againstEhrlichiaspp. were found in horses from one region (Northeastern) at a rate of 0.5% (1/192). Anti-B. burgdorferiantibodies were detected in all the three regions with a mean frequency of 15.1% (29/192), ranging from 14.06 to 17.19% by region. A co-exposure toA. phagocytophilumandB. burgdorferiwas observed in 6.3% of the cases (12/192). This is the first report on the natural exposure of horses to these bacteria (A. phagocytophilum, Ehrlichiaspp. andB. burgdorferi) in Northern Bulgaria.

scholarly journals Serological Evidence of Natural Exposure to Tick-Borne Pathogens in Horses, Romania

2021 ◽  
Vol 9 (2) ◽  
pp. 373
Author(s):  
Andreea Monica Bogdan ◽  
Mariana Ionita ◽  
Ioan Liviu Mitrea
Keyword(s):  
Confidence Interval ◽  
Borrelia Burgdorferi ◽  
Anaplasma Phagocytophilum ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Serological Evidence ◽  
North Central ◽  
Natural Exposure

The purpose of this study was to investigate the seroprevalence of selected tick-borne-pathogens (TBPs) among Romanian horses. For this, a total of 223 animals originating from north, central, and southeast Romania, including horses from stud farms (n = 118) and working horses (n = 105), were tested using a commercial rapid ELISA-based test. Overall, 10.3% (95% confidence interval (CI): 6.7–15.1%) of the tested horses were seropositive for antibodies (Ab) against Anaplasma phagocytophilum. Additionally, 18.8% (95% CI: 13.9–24.6%) and 0.5% (95% CI: 0.01–2.5%) of horses were seropositive for Ab against Borrelia burgdorferi sensu lato and Ehrlichia spp., respectively. Among the tested horses, 3.1% were seroreactive to two or three pathogens. These findings show the natural exposure of Romanian horses to zoonotic tick-borne pathogens and emphasize the need for further studies to better understand the epidemiology of equine tick-borne diseases in Romania.

scholarly journals Seroprevalence of antibodies against Anaplasma phagocytophilum and Borrelia burgdorferi in horses (Equus caballus) from northern Algeria

2020 ◽  
Vol 64 (3) ◽  
pp. 413-419
Author(s):  
Abdelouahab Laamari ◽  
Naouelle Azzag ◽  
Safia Tennah ◽  
Salima-Yamina Derdour ◽  
Bernard China ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Anaplasma Phagocytophilum ◽  
Bacterial Species ◽  
Antibody Test ◽  
Equus Caballus ◽  
Igg Antibodies ◽  
Blood Samples ◽  
Granulocytic Anaplasmosis ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

AbstractIntroductionHorses (Equus caballus) are susceptible to tick-borne diseases. Two of them, Lyme borreliosis due to Borrelia burgdorferi and granulocytic anaplasmosis due to Anaplasma phagocytophilum were investigated in Algerian horses. The diseases have been less extensively studied in horses and results pertinent to Algeria have not been published.Material and MethodsBlood samples were obtained from 128 horses. IgG antibodies directed against Anaplasma phagocytophilum and Borrelia burgdorferi were detected by an indirect immunofluorescence antibody test (IFAT) and ELISA. The potential effects of age, gender, breed, and health status on seropositivity were also evaluated.ResultsUsing IFAT, 28 (21.8%) and 25 (19.5%) animals were positive for B. burgdorferi and A. phagocytophilum, respectively. Using ELISA, 19 (14.8%) and 33 (25.9%) animals were positive for these bacteria.ConclusionThe study shows that horses in Algeria are exposed or co-exposed to tick-transmitted zoonotic bacterial species.

scholarly journals Retrospective Study Investigating the Seroprevalence of Anaplasma phagocytophilum in Manitoba, Canada: 2011–2014

2016 ◽  
Vol 3 (4) ◽  
Author(s):  
Kamran Kadkhoda ◽  
Ainsley Gretchen
Keyword(s):  
Retrospective Study ◽  
Anaplasma Phagocytophilum ◽  
Missed Opportunities ◽  
Human Granulocytic Anaplasmosis ◽  
First Report ◽  
True Incidence ◽  
Granulocytic Anaplasmosis

AbstractHuman granulocytic anaplasmosis is currently not nationally notifiable in Canada. This for the most part accounts for gross under-estimation of true incidence and prevalence of HGA and would potentially culminate in clinical missed opportunities. To the best of our knowledge, this is the first report on the seroprevalence of Anaplasma phagocytophilum in a Canadian jurisdiction with known established black-legged tick populations.

scholarly journals Granulocytic anaplasmosis in captive ring-tailed lemur (Lemur catta) in Poland

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Łukasz Adaszek ◽  
Anna Wilczyńska ◽  
Jerzy Ziętek ◽  
Marcin Kalinowski ◽  
Oliwier Teodorowski ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Polymerase Chain Reaction Test ◽  
Anaplasma Ovis ◽  
Case Presentation ◽  
Granulocytic Anaplasmosis ◽  
Pcr Product ◽  
Polymerase Chain ◽  
Obligate Intracellular Bacteria ◽  
Chain Reaction Test ◽  
Anaplasma Bovis

Abstract Background Anaplasma are obligate intracellular bacteria and aetiological agents of tick-borne diseases of both veterinary and medical interest. The genus Anaplasma comprises six species: Anaplasma marginale, Anaplasma centrale, Anaplasma ovis, Anaplasma phagocytophilum, Anaplasma bovis and Anaplasma platys. They can infect humans, carnivores, ruminants, rodents, insectivores, birds and reptiles. The aim of this study was to present the first clinical case of granulocytic anaplasmosis in a captive ring-tailed lemur in Poland. Case presentation A 4-year-old female lemur presented anorexia, epistaxis and tick infestation. The microscopic examination of a blood smear revealed morulae in neutrophils. Polymerase chain reaction test and sequencing of obtained PCR product confirmed infection by the GU183908 Anaplasma phagocytophilum strain. Therapeutic protocol included doxycycline (2.5 mg/kg p.o., b.i.d.) for 3 weeks and the lemur recovered within 24 h. Conclusions This is the first report on granulocytic anaplasmosis in a ring-tailed lemur in Europe, indicating that A. phagocytophilum infection must also be considered in differential diagnosis in this animal species, especially in individuals with thrombocytopenia associated with Ixodes ricinus parasitism.

Crinoids from the Muldraugh Member of the Borden Formation in north-central Kentucky (Echinodermata, Lower Mississippian)

2000 ◽  
Vol 74 (6) ◽  
pp. 1072-1082 ◽  
Author(s):  
William I. Ausich ◽  
Alan Goldstein ◽  
Ron Yates
Keyword(s):  
United States ◽  
New Species ◽  
Rank Order ◽  
Species Abundance ◽  
North Central ◽  
First Report ◽  
Eastern Side ◽  
Borden Formation ◽  
Depositional Setting ◽  
Mixed Carbonate

A new late Osagean (Lower Mississippian) crinoid fauna is described from the Muldraugh Member of the Borden Formation of central Kentucky. The Muldraugh Member records a mixed carbonate-siliciclastic tempestite-dominated ramp environment. This is the first report of late Osagean crinoids from this depositional setting on the eastern side of the Eastern Interior Basin of the midcontinental United States. The Muldraugh crinoid fauna contains 66 species and is dominated by advanced cladids. This fauna has similarities to other late Osagean faunas but is distinctive both in species abundance and rank order abundance of species. Eight new species are described from this fauna, includingCamptocrinus nudus, Paradichocrinus ramus, Poteriocrinites horowitzi, Parascytalocrinus pentagonus, Abrotocrinus debrae, Abrotocrinus springeri, Worthenocrinus hardinensis, andMespilocrinus myllos.

scholarly journals CXCR2 Blockade Influences Anaplasma phagocytophilum Propagation but Not Histopathology in the Mouse Model of Human Granulocytic Anaplasmosis

2004 ◽  
Vol 11 (5) ◽  
pp. 963-968 ◽  
Author(s):  
Diana G. Scorpio ◽  
Mustafa Akkoyunlu ◽  
Erol Fikrig ◽  
J. Stephen Dumler
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Tissue Injury ◽  
Treated Group ◽  
Obligate Intracellular Bacterium ◽  
Human Granulocytic Anaplasmosis ◽  
Obligate Intracellular ◽  
Liver Histopathology ◽  
Granulocytic Anaplasmosis ◽  
Infected Cells ◽  
And Control

ABSTRACT Anaplasma phagocytophilum is an obligate intracellular bacterium that infects neutrophils and causes human granulocytic anaplasmosis. Infection induces neutrophil secretion of interleukin-8 or murine homologs and perpetuates infection by recruiting susceptible neutrophils. We hypothesized that antibody blockade of CXCR2 would decrease A. phagocytophilum tissue load by interrupting neutrophil recruitment but would not influence murine hepatic pathology. C3H-scid mice were treated with CXCR2 antiserum or control prior to or on day 14 after infection. Quantitative PCR and immunohistochemistry for A. phagocytophilum were performed and severity of liver histopathology was ranked. Control mice had more infected cells in tissues than the anti-CXCR2-treated group. The histopathological rank was not different between treated and control animals. Infected cells of control mice clustered in tissue more than in treated mice. The results support the hypothesis of bacterial propagation through chemokine induction and confirm that tissue injury is unrelated to A. phagocytophilum tissue load.

scholarly journals Monoinfections caused by Borrelia burgdorferi and Borrelia burgdorferi / Anaplasma phagocytophilum co-infections in forestry workers and farmers

2015 ◽  
Vol 66 (5) ◽  
pp. 645-651 ◽  
Author(s):  
Małgorzata Tokarska-Rodak ◽  
Anna Pańczuk ◽  
Maria Kozioł-Montewka ◽  
Dorota Plewik ◽  
Adam Szepeluk
Keyword(s):  
Borrelia Burgdorferi ◽  
Anaplasma Phagocytophilum ◽  
Forestry Workers

scholarly journals Akutni perikarditis u koinfekciji uzročnicima Anaplasma phagocytophilum i Borrelia burgdorferi

2021 ◽  
Vol 41 (1) ◽  
pp. 27-29
Author(s):  
Samira Knežević ◽  
Irena Slavuljica ◽  
Anamarija Flego Bojić ◽  
Đurđica Cekinović-Grbeša ◽  
Lari Gorup
Keyword(s):  
Borrelia Burgdorferi ◽  
Ixodes Ricinus ◽  
Anaplasma Phagocytophilum

Lajmska borelioza i krpeljni meningoencefalitis (KME) najčešće su bolesti prenosive krpeljima u Republici Hrvatskoj. Isti vektor, krpelj roda Ixodes ricinus, odgovoran je i za prijenos humane granulocitne anaplazmoze (HGA) koja se u našoj zemlji rijetko dokazuje, a prvi slučajevi su potvrđeni 1998. godine u Koprivničko-križevačkoj županiji. HGA se najčešće klinički prezentira vrućicom s leukopenijom, trombocitopenijom, povišenim aminotransferazama i CRP-om, a potvrđuje se serološki, pri čemu se povremeno dokaže koinfekcija s virusom KME i/ili bakterijom Borrelia burgdorferi. Prikazujemo slučaj 44-godišnje bolesnice koja je ambulantno liječena u Klinici za infektivne bolesti Kliničkog bolničkog centra Rijeka zbog vrućice s bicitopenijom (leukopenija, trombocitopenija) i akutnog perikarditisa. Serološkom je obradom dokazana akutna koinfekcija uzročnicima Anaplasma phagocytophilum i Borrelia burgdorferi. Liječenje je provedeno doksiciklinom i nesteroidnim antireumaticima, čime je postignut povoljan klinički odgovor.

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