scholarly journals Identification of neuropeptide Y in superior cervical ganglion neurons that project to the oesophagus – A combined immunohistochemical labelling and retrograde tracing study in pigs

2019 ◽  
Vol 67 (1) ◽  
pp. 98-105 ◽  
Author(s):  
Liliana Rytel ◽  
Anna Snarska ◽  
Slawomir Gonkowski ◽  
Joanna Wojtkiewicz ◽  
Ottó Szenci ◽  
...  
Keyword(s):  
Neuropeptide Y ◽  
Superior Cervical Ganglion ◽  
Active Substance ◽  
Retrograde Tracing ◽  
Gi Tract ◽  
Neuronal Tracing ◽  
Cervical Oesophagus ◽  
Cervical Ganglion ◽  
Extrinsic Innervation ◽  
Ganglion Neurons

Neuropeptide Y (NPY) is a neuronal active substance taking part in the regulation of gastrointestinal (GI) tract activity. This study used retrograde neuronal tracing and immunofluorescence methods to analyse NPY-positive neurons located in superior cervical ganglion and supplying the cervical oesophagus in the pig. The presence of NPY was observed in 30% of all neurons supplying the part of oesophagus studied. Probably the number of Fast Blue (FB) positive cells depends on the area of the wall injected with FB and the fragment of oesophagus studied. Therefore, the obtained results indicate that the described peptide is an important factor in the extrinsic innervation of this part of the GI tract.

Immunohistochemical characterization of superior cervical ganglion neurons supplying porcine parotid salivary gland

2011 ◽  
Vol 500 (1) ◽  
pp. 57-62 ◽  
Author(s):  
Joanna Wojtkiewicz ◽  
Judyta K. Juranek ◽  
Ireneusz Kowalski ◽  
Marek Bladowski ◽  
Jarosław Całka ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Superior Cervical Ganglion ◽  
Parotid Salivary Gland ◽  
Cervical Ganglion ◽  
Ganglion Neurons

Labelling and recording from dissociated target-specific rat superior cervical ganglion neurons

1992 ◽  
Vol 135 (2) ◽  
pp. 210-214 ◽  
Author(s):  
Jennifer I. Luebke ◽  
Forrest F. Weight ◽  
Luis G. Aguayo
Keyword(s):  
Superior Cervical Ganglion ◽  
Cervical Ganglion ◽  
Ganglion Neurons

scholarly journals Nerve Fibers Containing Neuropeptide Y in the Cerebrovascular Bed: Immunocytochemistry, Radioimmunoassay, and Vasomotor Effects

1987 ◽  
Vol 7 (1) ◽  
pp. 45-57 ◽  
Author(s):  
L. Edvinsson ◽  
J. R. Copeland ◽  
P. C. Emson ◽  
J. McCulloch ◽  
R. Uddman
Keyword(s):  
Neuropeptide Y ◽  
Blood Vessels ◽  
Superior Cervical Ganglion ◽  
Cerebral Arteries ◽  
Nerve Fibers ◽  
Calcium Entry Blocker ◽  
Cervical Ganglion ◽  
Prostaglandin F ◽  
6 Hydroxydopamine

Perivascular nerve fibers containing neuropeptide Y (NPY)-like immunoreactivity were identified around cerebral blood vessels of human, cat, guinea pig, rat, and mouse. The major cerebral arteries were invested by dense plexuses; veins, small arteries, and arterioles were accompanied by few fibers. Removal of the superior cervical ganglion resulted in a reduction of NPY-like material in pial vessels and dura mater. Pretreatment with 6-hydroxydopamine or reserpine reduced the number of visible NPY fibers and the concentration of NPY in rat cerebral vessels. Sequential immuno-staining with antibodies toward dopamine-β-hydroxylase (DBH) (an enzyme involved in the synthesis of noradrenaline) and NPY revealed an identical localization of DBH and NPY in nerve cell bodies in the superior cervical ganglion and in perivascular fibers of pial blood vessels, suggesting their coexistence. Administration of NPY in vitro resulted in concentration-dependent contractions that were not modified by a sympathectomy. The contractions induced by noradrenaline, 5-hydroxytryptamine, and prostaglandin F2α and the dilator responses to calcitonin gene-related peptide were not modified by NPY in rat cerebral arteries. However, the constrictor response to NPY was reduced by 70% in the presence of the calcium entry blocker nifedipine, and abolished following incubation in a calcium-free buffer. These data suggest an interaction of NPY at a postsynaptic site, which for induction of contraction may open calcium channels in the sarcolemma of cerebral arteries.

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