scholarly journals Quick cytogenetic screening of breeding bulls using flow cytometric sperm DNA histogram analysis

2016 ◽  
Vol 64 (3) ◽  
pp. 372-379
Author(s):  
Szabolcs Nagy ◽  
Péter J. Polgár ◽  
Magnus Andersson ◽  
András Kovács
Keyword(s):  
Semen Quality ◽  
Size Variation ◽  
Genome Size Variation ◽  
Dna Analyses ◽  
Flow Cytometric ◽  
Cytogenetic Aberrations ◽  
Sperm Dna ◽  
Series Of Experiments ◽  
Baseline Values ◽  
Dna Histogram

The aim of the present study was to test the FXCycle PI/RNase kit for routine DNA analyses in order to detect breeding bulls and/or insemination doses carrying cytogenetic aberrations. In a series of experiments we first established basic DNA histogram parameters of cytogenetically healthy breeding bulls by measuring the intraspecific genome size variation of three animals, then we compared the histogram profiles of bulls carrying cytogenetic defects to the baseline values. With the exception of one case the test was able to identify bulls with cytogenetic defects. Therefore, we conclude that the assay could be incorporated into the laboratory routine where flow cytometry is applied for semen quality control.

scholarly journals Flow cytometric analysis of genome size variation in some Passiflora species

Hereditas ◽  
2004 ◽  
Vol 141 (1) ◽  
pp. 31-38 ◽  
Author(s):  
MARGARETE MAGALHÃES SOUZA ◽  
GUADALUPE PALOMINO ◽  
TELMA NAIR SANTANA PEREIRA ◽  
MESSIAS GONZAGA PEREIRA ◽  
ALEXANDRE PIO VIANA
Keyword(s):  
Genome Size ◽  
Flow Cytometric Analysis ◽  
Size Variation ◽  
Genome Size Variation ◽  
Flow Cytometric

scholarly journals Genome size variation in Deschampsia cespitosa sensu lato (Poaceae) in Eurasia

2022 ◽  
Vol 308 (1) ◽  
Author(s):  
Josef Greimler ◽  
Eva M. Temsch ◽  
Zhiqing Xue ◽  
Hanna Weiss-Schneeweiss ◽  
Polina Volkova ◽  
...  
Keyword(s):  
Genome Size ◽  
Size Variation ◽  
Genome Size Variation ◽  
Geographic Scale ◽  
Reproduction Mode ◽  
Ploidy Levels ◽  
Deschampsia Cespitosa ◽  
Flow Cytometric ◽  
Significant Difference ◽  
Disturbed Habitats

AbstractThe grass Deschampsia cespitosa is a variable taxon out of which many varieties, subspecies and endemic species have been separated. In this paper, the variation in genome size (GS) and ploidy of this grass including several of its subspecies and two related species in Eurasia was investigated by flow cytometric (FCM) measurements. GS and ploidy data were also related to specific environments and reproduction mode. Ploidy levels found by FCM were confirmed by chromosome counts of diploid (2n = 28) and tetraploid (2n = 52) samples. Seminiferous (seed bearing) D. cespitosa was mainly diploid (GS between 3.754 and 5.438 pg/1C). GS variation in diploids showed a geographic pattern with a significant difference (H = 41,441, P < 0.001) between European (median = 4.377 pg) and Asian (median = 4.881 pg) accessions. Genome size (1C) in tetraploids ranged from 7.9426 to 9.0399 pg. Tetraploid seminiferous D. cespitosa was found mostly in disturbed habitats in western and southern Europe, while tetraploids in Asia were registered in wet Arctic habitats. Genome size (1C between 8.3278 and 8.8603 pg) of the pseudoviviparous plants (spikelets produce plantlets asexually) of wet habitats in central and northern Europe indicated tetraploidy. A putative triploid (GS 6.6817 pg) was detected in Iceland. Summing up, we found a high variation in GS on the geographic scale with significant regional differences in diploid D. cespitosa. Among the tetraploids, the asexually reproducing plants were bound to specific habitats, while the seminiferous plants showed a habitat preference similar to the diploids.

scholarly journals Genome Size Variation among and within Camellia Species by Using Flow Cytometric Analysis

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e64981 ◽  
Author(s):  
Hui Huang ◽  
Yan Tong ◽  
Qun-Jie Zhang ◽  
Li-Zhi Gao
Keyword(s):  
Genome Size ◽  
Flow Cytometric Analysis ◽  
Size Variation ◽  
Genome Size Variation ◽  
Flow Cytometric ◽  
Camellia Species

Flow cytometric DNA analyses of frozen samples from children's solid tumors

Pathology ◽  
1993 ◽  
Vol 25 (4) ◽  
pp. 388-393 ◽  
Author(s):  
Christine M. Smyth ◽  
Megan A. Helmer ◽  
Luciano Dalla Pozza ◽  
Peter B Rowe
Keyword(s):  
Solid Tumors ◽  
Dna Analyses ◽  
Flow Cytometric

Documenting Intraspecfic Genome Size Variation in Soybean

Crop Science ◽  
2004 ◽  
Vol 44 (1) ◽  
pp. 261 ◽  
Author(s):  
A. Lane Rayburn ◽  
D. P. Biradar ◽  
R. L. Nelson ◽  
R. McCloskey ◽  
K. M. Yeater
Keyword(s):  
Genome Size ◽  
Size Variation ◽  
Genome Size Variation

scholarly journals Genome size variation in deep-sea amphipods

2017 ◽  
Vol 4 (9) ◽  
pp. 170862 ◽  
Author(s):  
H. Ritchie ◽  
A. J. Jamieson ◽  
S. B. Piertney
Keyword(s):  
Life History ◽  
Genome Size ◽  
Deep Sea ◽  
Research Effort ◽  
Life History Strategy ◽  
Size Variation ◽  
Genome Size Variation ◽  
New Hebrides ◽  
Contrast Analysis ◽  
Independent Contrast

Genome size varies considerably across taxa, and extensive research effort has gone into understanding whether variation can be explained by differences in key ecological and life-history traits among species. The extreme environmental conditions that characterize the deep sea have been hypothesized to promote large genome sizes in eukaryotes. Here we test this supposition by examining genome sizes among 13 species of deep-sea amphipods from the Mariana, Kermadec and New Hebrides trenches. Genome sizes were estimated using flow cytometry and found to vary nine-fold, ranging from 4.06 pg (4.04 Gb) in Paralicella caperesca to 34.79 pg (34.02 Gb) in Alicella gigantea . Phylogenetic independent contrast analysis identified a relationship between genome size and maximum body size, though this was largely driven by those species that display size gigantism. There was a distinct shift in the genome size trait diversification rate in the supergiant amphipod A. gigantea relative to the rest of the group. The variation in genome size observed is striking and argues against genome size being driven by a common evolutionary history, ecological niche and life-history strategy in deep-sea amphipods.

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