scholarly journals Mast cell distribution around the needle tract following acupuncture in Zusanli (ST36) acupoint in rats

2019 ◽  
Vol 22 (1) ◽  
pp. 91-98 ◽  
Author(s):  
N. D Dimitrov ◽  
D. Y. Atanasova ◽  
N. S. Tomov ◽  
Y. A. Staykova-Pirovska ◽  
I. G. Ivanova ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Mast Cell ◽  
Connective Tissue ◽  
Cell Distribution ◽  
Striated Muscles ◽  
Needle Tract ◽  
Transmission Electron ◽  
Subcutaneous Connective Tissue

The aim of this study was to investigate mast cell (MCs) distribution in the vicinity of the needle tract formed after acupuncture in Zusanli (ST36) acupoint in rats. MCs were detected by histochemistry, immunohistochemistry and transmission electron microscopy, and evaluated quantitatively. It was established that after acupuncture in ST36 acupoint the integrity of the epithelium, dermis, subcutaneous connective tissue, fascia, epimysium and striated muscles was disrupted and folded to the direction of the needle tract. In the thickened connective tissue MCs were observed close to the needle tract, without visible differences in their number along the tract, but most of them were with signs of degranulation, possibly due to acupuncture. It could be presumed that acupuncture in ST36 caused recruitment and activation of MCs followed by degranulation which most probably influenced the local microenvironment.

Magnesium and sulfur in mast cell and basophil granules of lower vertebrates in relation with histamine

1992 ◽  
Vol 50 (2) ◽  
pp. 1596-1597
Author(s):  
Kenichi Takaya
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Mast Cell ◽  
Mast Cells ◽  
Tree Frog ◽  
X Ray ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Fresh Frozen ◽  
Ultrathin Sections

Mast cell and basophil granules of the vertebrate contain heparin or related sulfated proteoglycans. Histamine is also present in mammalian mast cells and basophils. However, no histamine is detected in mast cell granules of the amphibian or fish, while it is shown in those of reptiles and birds A quantitative x-ray microanalysis of mast cell granules of fresh frozen dried ultrathin sections of the tongue of Wistar rats and tree frogs disclosed high concentrations of sulfur in rat mast cell granules and those of sulfur and magnesium in the tree frog granules. Their concentrations in tree frog mast cell granules were closely correlated (r=0.94).Fresh frozen dried ultrathin sections and fresh air-dried prints of the tree frog tongue and spleen and young red-eared turtle (ca. 6 g) spleen and heart blood were examined by a quantitative energy-dispersive x-ray microanalysis (X-650, Kevex-7000) for the element constituents of the granules of mast cells and basophils. The specimens were observed by transmission electron microscopy (TEM) (80-200 kV) and followed by scanning transmission electron microscopy (STEM) under an analytical electron microscope (X-650) at an acceleration voltage of 40 kV and a specimen current of 0.2 nA. A spot analysis was performed in a STEM mode for 100 s at a specimen current of 2 nA on the mast cell and basophil granules and other areas of the cells. Histamine was examined by the o-phthalaldehyde method.

Fine structure of dendritic cells in the epithelial cell layer of the ox tongue

1990 ◽  
Vol 48 (3) ◽  
pp. 528-529
Author(s):  
Kan Kobayashi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Dendritic Cells ◽  
Epithelial Cell ◽  
Connective Tissue ◽  
Cell Layer ◽  
Transmission Electron ◽  
Epithelial Cell Layer ◽  
Scanning Electron

It is known that some kinds of dendritic cells are distributed in the epithelial cell layer of mucous membrane consisting of stratified squamous epithelium. In the process of exfoliation of the epithelial layer from the underlying connective tissue, dendritic cell bodies exposed on the ruptured surface of the epithelium were detected by scanning electron microscopy. These cells were also observed by light microscopy as well as by transmission electron microscopy.Dorsal mucous membrane of the ox tongue was fixed in Karnovsky's fixative or in 10% formalin. For scanning electron microscopy samples were immersed in 3N-HCl solution for 2-3 weeks at room temperature. The epithelial cell layer was removed from the underlying connective tissue layer1). They were postfixed in tannic acid and then 1% OsO4 for 1 hr. After dehydration in an ethanol series, the specimens were dried by passing through t-butylalcohol, coated with platinum-palladium and observed under an S-800 scanning electron microscope. For transmission electron microscopy, small pieces of the fixed tissue were post-fixed in 1% OsO4 for 1.5 hr and then embedded in Araldite-Epon.

Ultrastructure of Lymphatic Stomata in the Tunica Vaginalis of Humans

2013 ◽  
Vol 19 (6) ◽  
pp. 1405-1409 ◽  
Author(s):  
Jiaxiong Wang ◽  
Zepeng Ping ◽  
Tingting Jiang ◽  
Hongyuan Yu ◽  
Chong Wang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Connective Tissue ◽  
Tumor Metastasis ◽  
Lymphatic System ◽  
Mesothelial Cell ◽  
Tunica Vaginalis ◽  
Pathological Conditions ◽  
Transmission Electron ◽  
Naoh Solution

AbstractLymphatic stomata are small openings of lymphatic capillaries on the surface of the mesothelium that lines the serous cavity and have the function of active absorption. They play an important role in physiological and pathological conditions. The cavity of the tunica vaginalis is a typical serous cavity of the testis, but the lymphatic stomata of the tunica vaginalis of humans have never been reported. Here, we studied their ultrastructure by scanning and transmission electron microscopy. The submesothelial connective tissue with foramina was investigated after the mesothelial cells were digested using NaOH solution. We found the lymphatic stomata in cuboidal mesothelial cell regions of the parietal layer of the tunica vaginalis of humans with a diameter of about 1–2 μm. Sometimes, closed lymphatic stomata could be observed. Our study is the first to report the existence of lymphatic stomata of the tunica vaginalis of humans. We found that the tunica vaginalis cavity is connected with the lymphatic system through the stomata, which might provide a morphological basis for the drainage of hydrocele and tumor metastasis of the tunica vaginalis of humans.

Carbon dioxide laser turbinectomy. An electron microscopic study

1997 ◽  
Vol 111 (10) ◽  
pp. 931-934 ◽  
Author(s):  
Samy Elwany ◽  
M. Hesham Abdel-Moneim
Keyword(s):  
Electron Microscopy ◽  
Carbon Dioxide ◽  
Transmission Electron Microscopy ◽  
Allergic Rhinitis ◽  
Connective Tissue ◽  
Clinical Response ◽  
Carbon Dioxide Laser ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Non Allergic Rhinitis

AbstractTen patients with chronic non-allergic rhinitis and presenting mainly with nasal obstruction due to hypertrophied inferior turbinates were subjected to laser turbinectomy using CO2. Tiny biopsies were taken, at the time of surgery as well as one monthlater, and were processed for transmission electron microscopy. The ultrastructural observations included: early epithelial loss which wasfollowed by prompt regeneration of healthy epithelium, decreased number and activity of the seromucinous glands, fibrosis of the connective tissue stroma, as well as diminished number and congestion of the cavernous blood spaces. These observations were discussed in view of the excellent clinical response of the patients.

Elemental composition of mast cell granules of the rat and tree frog: X-ray Microanalysis on fresh frozen dried ultrathin sections

1990 ◽  
Vol 48 (3) ◽  
pp. 356-357
Author(s):  
Kenichi Takaya
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Mast Cell ◽  
Mast Cells ◽  
Pancreatic Acinar Cells ◽  
Tree Frog ◽  
X Ray ◽  
Transmission Electron ◽  
Fresh Frozen ◽  
Ultrathin Sections

Electron probe x-ray microanalysis using fresh air-dried spreads revealed electrolyte elements in the granules of platelets, mast cells, pancreatic acinar cells and melanocytes. Mast cell granules of the rat and tree frog are quantitatively compared by an energy dispersive spectrometry (EDS) using fresh frozen dried ultrathin sections.Adult Wistar rats (ca. 250 g) and tree frogs (Hyla arborea japonica) of both sexes were used. Fresh frozen dried smears of rat peritoneal mast cells were prepared on the collodion-membrane covered titanium grid. Fresh frozen dried ultrathin sections of the tongue of the rat and tree frog were made by the metal contact method employing the rapid freezing apparatus (RF-2) cooled with liquid nitrogen. Ultrathin cryosections (60 nmj were cut at 163 K, transferred to the frozen specimen treating apparatus (FD-2) for freeze-drying in high vacuum at 173 K for 74-96 h. The specimens were observed first by 200kV transmission electron microscopy (TEM) and then under the scanning analytical electron microscope (X-650) for scanning transmission electron microscopy (STEM) images at an acceleration voltage of 40 kV and a specimen current of 0.2 nA.

scholarly journals Premature ovarian failure: morphological and ultrastructural aspects

1994 ◽  
Vol 112 (2) ◽  
pp. 534-538 ◽  
Author(s):  
Mauro Abi Haidar ◽  
Edmund Chada Baracat ◽  
Manuel de Jesus Simões ◽  
Gustavo Rubino de Azevedo Focchi ◽  
Joaquim Evêncio Neto ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Connective Tissue ◽  
Corpus Luteum ◽  
Premature Ovarian Failure ◽  
Ovarian Follicles ◽  
Ovarian Failure ◽  
Primordial Follicles ◽  
Dense Connective Tissue ◽  
Transmission Electron

The authors documented by means of light and transmission electron microscopy that the ovaries of women with premature ovarian failure (POF) displayed dense connective tissue and rare corpora albicantia. Eight of the ten studied cases did not present ovarian follicles; in two cases, it was verified the presence of ovarian follicles, atypical primordial follicles and in one case, a corpus luteum was identified (after stimulation with exogenous gonadotrophin). Regarding the ultrastructural analysis, it was noted that the fibroblasts were united one to each other by cellular prolongations that formed a woof, constituting a cellular syncicius.

Techniques for Transmission Electron Microscopy of Fiber-Matrix Interfaces in Aluminum Alloy-Boron Composites by Ion Erosio

1971 ◽  
Vol 29 ◽  
pp. 204-205
Author(s):  
G. G. Shaw
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Aluminum Alloy ◽  
Electron Beam ◽  
Pure Aluminum ◽  
Ion Milling ◽  
Transmission Electron ◽  
Fiber Matrix ◽  
Ion Erosion ◽  
Row Of Fibers

The morphology and composition of the fiber-matrix interface can best be studied by transmission electron microscopy and electron diffraction. For some composites satisfactory samples can be prepared by electropolishing. For others such as aluminum alloy-boron composites ion erosion is necessary.When one wishes to examine a specimen with the electron beam perpendicular to the fiber, preparation is as follows: A 1/8 in. disk is cut from the sample with a cylindrical tool by spark machining. Thin slices, 5 mils thick, containing one row of fibers, are then, spark-machined from the disk. After spark machining, the slice is carefully polished with diamond paste until the row of fibers is exposed on each side, as shown in Figure 1.In the case where examination is desired with the electron beam parallel to the fiber, preparation is as follows: Experimental composites are usually 50 mils or less in thickness so an auxiliary holder is necessary during ion milling and for easy transfer to the electron microscope. This holder is pure aluminum sheet, 3 mils thick.

Direct Observation of Heat Exchanger Deposits by Cross Sectioning

1967 ◽  
Vol 25 ◽  
pp. 364-365
Author(s):  
R. W. Anderson ◽  
D. L. Senecal
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Heat Exchanger ◽  
Direct Observation ◽  
Cross Sections ◽  
Preparation Method ◽  
Specimen Preparation ◽  
Flowing Water ◽  
Transmission Electron ◽  
Deposit Layer

A problem was presented to observe the packing densities of deposits of sub-micron corrosion product particles. The deposits were 5-100 mils thick and had formed on the inside surfaces of 3/8 inch diameter Zircaloy-2 heat exchanger tubes. The particles were iron oxides deposited from flowing water and consequently were only weakly bonded. Particular care was required during handling to preserve the original formations of the deposits. The specimen preparation method described below allowed direct observation of cross sections of the deposit layers by transmission electron microscopy.The specimens were short sections of the tubes (about 3 inches long) that were carefully cut from the systems. The insides of the tube sections were first coated with a thin layer of a fluid epoxy resin by dipping. This coating served to impregnate the deposit layer as well as to protect the layer if subsequent handling were required.

Phase Transformations in Ti-7w/oMo-3w/oMe Alloy

1974 ◽  
Vol 32 ◽  
pp. 514-515
Author(s):  
S. Fujishiro
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Transmission Electron Microscopy ◽  
Tensile Strength ◽  
Mechanical Processing ◽  
Ray Method ◽  
X Ray ◽  
Transmission Electron ◽  
Water Quench ◽  
Alpha Beta

The mechanical properties of three titanium alloys (Ti-7Mo-3Al, Ti-7Mo- 3Cu and Ti-7Mo-3Ta) were evaluated as function of: 1) Solutionizing in the beta field and aging, 2) Thermal Mechanical Processing in the beta field and aging, 3) Solutionizing in the alpha + beta field and aging. The samples were isothermally aged in the temperature range 300° to 700*C for 4 to 24 hours, followed by a water quench. Transmission electron microscopy and X-ray method were used to identify the phase formed. All three alloys solutionized at 1050°C (beta field) transformed to martensitic alpha (alpha prime) upon being water quenched. Despite this heavily strained alpha prime, which is characterized by microtwins the tensile strength of the as-quenched alloys is relatively low and the elongation is as high as 30%.

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