scholarly journals COMPARISON OF A RAPID ENZYME-LINKED IMMUNOSORBANT ASSAY TEST WITH AN INDIRECT IMMUNOFLUORESCENT ANTIBODY TEST IN DIAGNOSING EHRLICHIA AND ANAPLASMA INFECTIONS IN DOGS

2019 ◽  
Vol 17 (4) ◽  
pp. 346-352
Author(s):  
Kr. Gospodinova ◽  
G. Zhelev ◽  
V. Petrov
Keyword(s):  
Serum Antibody ◽  
Rapid Test ◽  
Antibody Test ◽  
Diagnostic Value ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Immunofluorescent Antibody ◽  
Antibody Titers ◽  
Indirect Immunofluorescent ◽  
Immunofluorescent Antibody Test

PURPOSE: The objective of this study is to compare the diagnostic value of commercial enzyme-linked immunosorbent assay (ELISA) with indirect immunofluorescent antibody test (IFA) in detecting immunoglobulin-G (IgG) antibodies to Ehrlichia canis and Anaplasma phagocytophilum. METHODS: Seventy-four serum samples, obtained from dogs believed to be naturally infected with E. canis or A. phagocytophilum, were analyzed. RESULTS: By ELISA, 48 (64.9%) samples were found positive for IgG to E. canis, 10 (13.5%) to A. phagocytophilum, 12 (16.2%) to both E. canis and A. phagocytophilum, and in 4 (5.4%) samples no presence of antibodies was detected. The number of serologically positive dogs for IgG was 44 (59.5%) to E. canis, 10 (13.5%) to A. phagocytophilum, 16 (21.6%) to both E. canis and A. phagocytophilum, and 4 (5.4%) were determined negative by means of IFA. In most samples the antibody titer did not exceed 1:80 but in 5 it reached a level of 1:320, and in other 4 of even above 1:640. CONCLUSIONS: This study shows that IFA assay is more sensitive than commercial ELISA rapid test when serum antibody titers are low.

scholarly journals Serodiagnosis of Visceral Leishmaniasis in Northeastern Italy: Evaluation of Seven Serological Tests

2020 ◽  
Vol 8 (12) ◽  
pp. 1847
Author(s):  
Margherita Ortalli ◽  
Daniele Lorrai ◽  
Paolo Gaibani ◽  
Giada Rossini ◽  
Caterina Vocale ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Western Blot ◽  
Rapid Test ◽  
Antibody Test ◽  
Screening Tests ◽  
Serum Samples ◽  
Serological Tests ◽  
Northeastern Italy ◽  
Indirect Immunofluorescent ◽  
Immunofluorescent Antibody Test

This study compares the performance of seven assays, including two ELISA (Leishmania ELISA IgG + IgM, Vircell Microbiologists; Leishmania infantum IgG ELISA, NovaTec), three rK39-based immunochromatographic tests (rK39-ICTs) (Leishmania Dipstick Rapydtest, Apacor; On Site Leishmania IgG/IgM Combo Rapid Test, CTK Biotech; LEISHMANIA Strip quick Test, Cypress Diagnostic), one indirect immunofluorescent antibody test (IFAT) (Leishmania-Spot IF, BioMérieux), and one western blot (WB) (Leishmania WESTERN BLOT IgG, LDBio Diagnostics) for serodiagnosis of visceral leishmaniasis (VL). Serum samples from 27 VL patients living in northeastern Italy were analyzed, as well as the serum samples from 50 individuals in whom VL diagnosis was excluded. The WB and the IFAT had 96% sensitivity, followed by the ELISA (63% and 74%, respectively). The rK39-ICT exhibited the worst performance among the serological tests, with sensitivities ranging from 52% to 70%. By combining selected ELISA/ICT, the sensitivity of VL detection reached 89%. IFAT and WB outperformed ELISA and rK39-ICT by possessing optimal sensitivity, but their high cost and complexity of execution would not allow their employment as screening tests. In conclusion, the combination of easy-to-perform tests, such as ICT and ELISA, could improve sensitivity in the serodiagnosis of Mediterranean VL.

scholarly journals Intra-uterine exposure of horses to Sarcocystis spp. antigens

2016 ◽  
Vol 68 (2) ◽  
pp. 271-275 ◽  
Author(s):  
A.M. Antonello ◽  
G.C. Cadore ◽  
F.L. Pivoto ◽  
G. Camillo ◽  
P. Braunig ◽  
...  
Keyword(s):  
Specific Antibody ◽  
Antibody Test ◽  
Immunoblot Analysis ◽  
Antibody Responses ◽  
Southern Brazil ◽  
Serum Samples ◽  
Immunofluorescent Antibody ◽  
Sarcocystis Spp ◽  
Indirect Immunofluorescent ◽  
Immunofluorescent Antibody Test

The aim of this study was to examine the intra-uterine exposure to Sarcocystis spp. antigens, determining the number of foals with detectable concentrations of antibodies against these agents in the serum, before colostrum ingestion and collect data about exposure of horses to the parasite. Serum samples were collected from 195 thoroughbred mares and their newborns in two farms from southern Brazil. Parasite specific antibody responses to Sarcocystis antigens were detected using the indirect immunofluorescent antibody test (IFAT) and immunoblot analysis. In 84.1% (159/189) of the pregnant mares and in 7.4% (14/189) of foals we detected antibodies anti-Sarcocystis spp. by IFAT. All samples seropositive from foals were also positive in their respective mares. Serum samples of seropositive foals by IFAT, showed no reactivity on the immunoblot, having as antigens S. neurona merozoites. In conclusion, the intra-uterine exposure to Sarcocystis spp. antigens in horses was demonstrated, with occurrence not only in mares, but also in their foals, before colostrum ingestion these occurrences were reduced.

scholarly journals Toxoplasma gondii antibodies on domiciled cats from Lages municipality, Santa Catarina State, Brazil

2010 ◽  
Vol 19 (4) ◽  
pp. 268-269 ◽  
Author(s):  
Luciana Dalla Rosa ◽  
Anderson Barbosa de Moura ◽  
Natascha Trevisani ◽  
Alessandra Pereira Medeiros ◽  
Amélia Aparecida Sartor ◽  
...  
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Rural Areas ◽  
Antibody Test ◽  
Southern Brazil ◽  
Santa Catarina ◽  
Factors Associated ◽  
Immunofluorescent Antibody ◽  
Indirect Immunofluorescent ◽  
Immunofluorescent Antibody Test

Sera were collected from 300 domiciled cats from the municipality of Lages, Southern Brazil, to determine the prevalence of Toxoplasma gondii antibodies and risk factors associated. Tests for T. gondii antibodies were performed using indirect immunofluorescent antibody test (IFAT). Positive reactions with titers >1:64 were found in 43 (14.33%) cats. A significant number of seropositive cats were >6 month old (p = 0.03758) and had access to the streets or/and rural areas (p = 0.04185). The results indicate that T. gondii is widespread in cats in Lages with a prevalence of 14.33%.

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