scholarly journals Бактерицидні та дезінфікуючі властивості деззасобу «Арквадез-плюс»

2017 ◽  
Vol 19 (78) ◽  
pp. 68-73
Author(s):  
O.L. Tishyn ◽  
G.T. Kopijchuk ◽  
R.V. Khomiak ◽  
O.V. Khyrivskyy ◽  
Т.V. Orynchak
Keyword(s):  
Cell Death ◽  
Production Environment ◽  
Spore Form ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Test Culture ◽  
Bactericidal Properties ◽  
Working Solution ◽  
Light Color ◽  
Rigid Surfaces

The results of research bactericidal properties of new domestic disinfectant «Arquadez–plus» which is a transparent light color, without mechanical inclusions, solution with a specific odor, and created on the basis of quaternary ammonium compounds (QAC), in particular, the composition of the disinfectant include: alkyldimethylbenzylammonium chloride and dimethyldipetylammonium chloride. Was found bactericidal dilution (BD), bactericidal concentration (BC), phenol coefficient (PC) and protein index (PI). Most susceptible to the effect of disinfectant was gram-negativ bacteria of E. coli, where cell death came on the 10 and 30 minutes exposures at concentrations of 0.0129 and 0.0092%, respectively. The action of disinfection preparation for gram-positive bacteria S. aureus, cell death came at these exposures at concentrations of 0.0252 and 0.018%, respectively. Microorganisms P. vulgaris were the least susceptible to the action of the disinfectant; the death of the microorganisms came at concentrations of 1,0204 and 0.5206%, for 10 and 30 minute exposures, respectively, and, due to the action of the disinfectant on the test culture of S. typhimurium, the death of cells came under given conditions, at concentrations of 0.5206 and 0.2656%, respectively. The action of disinfection preparation for vegetative cells of B. subtilis cell death came on the 10 and 30 minutes exposures at concentrations of 0.0494 and 0.018% but in the spore form, at concentrations of 0.5206 and 0.3719%, respectively. The disinfectant effect of this disinfectant is 79.4 and 28.9 times higher on microorganisms E. coli and S. aureus, respectively, of the decontaminant effect of phenol, and in the presence of a protein, the activity of the disinfectant is reduced by 1.4 times. The efficacy of disinfection preparation of microorganisms on surfaces in the decontamination of test-objects.It is established that test-cultures of E. coli, S. aureus and P. vulgaris at 0.05% concentration of disinfectant is ineffective and for the test culture of S. typhimurium, its 0.1% concentration is ineffective.For vegetative and spore form of B. subtilis disinfection preparation concentration at 1.0%. Is ineffective at the 0.5–1.0% concentrations is effective for processing rigid surfaces, at preventive disinfection of premises for animals and birds at exposure for 60 minutes, and at spore forms of microorganisms its working concentration should be 2.0%, at exposure in 120 minutes and 3.0% at exposures in 30 minutes or more. Proved that the disinfectant «Arquadez-plus» in a production environment at a concentration of 1.0% by wet surface irrigation facilities for animals and working solution 0.25–0.3 liters per 1 square meter with an exposure of 2 hours, showing high disinfectant properties. 

scholarly journals Bactericidal and disinfective properties of disinfectant «Zyrocko-400-Glucuxid»

2018 ◽  
Vol 20 (83) ◽  
pp. 255-259
Author(s):  
O.L. Tishyn ◽  
R.V. Khomiak ◽  
G.T. Kopijchuk ◽  
Т.V. Orynchak ◽  
O.-I.Y. Galutskiy
Keyword(s):  
Cell Death ◽  
Spore Form ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Test Culture ◽  
Bactericidal Properties ◽  
Light Color ◽  
Ammonium Compounds ◽  
Specific Odor ◽  
Test Objects

The results of research of bactericidal properties of new domestic disinfectant «Zyrocko-400-Glucuxid» which is a transparent light color, without mechanical inclusions, a solution with a specific odor. It is well mixed with water, created on the basis of Quaternary Ammonium Compounds (QAC), glutaraldehyde and purified water. It was found the bactericidal dilution (BD), bactericidal concentration (BC), phenol coefficient (PC) and protein index (PI). Most susceptible to the effect of disinfectant was gramnegativ bacteria of E. coli, where cell death came on the 10 and 30 minutes exposures at concentrations of 0.0691 and 0.0353%, respectively. The action of disinfection preparation for gram-positive bacteria S. aureus, cell death came at these exposures at concentrations of 0.1355 and 0.0691%, respectively. Microorganisms S. typhimurium were the least susceptible to the action of the disinfectant; the death of the microorganisms came at concentrations of 1.4286 and 1.0204%, for 10 and 30 minute exposures, respectively, and, due to the action of the disinfectant on the test culture of P. vulgaris, the death of cells came under given conditions, at concentrations of 0.7289 і 0.5206%, respectively. The action of disinfection preparation for spore form of B. subtilis cell death came on the 10 and 30 minutes exposures at concentrations of 1.4286 і 0.7289%, respectively. The disinfectant effect of this disinfectant is 17.71 і 6.46  times higher on microorganisms E. coli and S. aureus, respectively, of the decontaminant effect of phenol, and in the presence of a protein, the activity of the disinfectant is reduced by 9.04 times. The efficacy of disinfection preparation of microorganisms on surfaces in the decontamination of test-objects. It is established that test-cultures of E. coli, S. aureus and P. vulgaris at 0.05% concentration of disinfectant is ineffective and for the test culture of S. typhimurium, its 0.5% concentration is ineffective. For spore form of B. subtilis disinfection preparation concentration at 1.0%. The preparation «Zуroсko-400-Glucuxide» is effective at disinfection of objects that are subject to a vet control at a concentration of 1.0%, subject to an exposure of 60 minutes or more. For decontamination of objects at spore forms of microorganisms, the working concentration of the insoluble must be 2.0% and above, at exposures of 60 minutes or more and 3.0% at exposures of 30 minutes or more.

scholarly journals Bactericidal and disinfective properties of disinfectant «GK-10»

2018 ◽  
Vol 20 (87) ◽  
pp. 3-7
Author(s):  
O.L. Tishyn ◽  
R.V. Khomiak ◽  
G.T. Kopijchuk ◽  
M.M. Danko ◽  
S.A. Ponomariova
Keyword(s):  
Cell Death ◽  
Animal Origin ◽  
Spore Form ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Light Yellow ◽  
Laboratory Facilities ◽  
Yellow Color ◽  
Bactericidal Properties ◽  
Light Yellow Color

The article presents the results of research on bactericidal properties of the new domestic disinfectant «GK-10», which is a transparent solution of light yellow color, without mechanical inclusions with a specific odor. It is well mixed with water. It is based on alkyl dimethylbenzyl ammonium chloride (QAC) and dodecylbispropylentriamine (1,3-prodadiene) (tertiary amines). Was found bactericidal dilution (BD), bactericidal concentration (BC), phenolic coefficient (FC) and protein index (PI). Thus, the most susceptible to the action of the disinfectant were were gram-negative bacteria E. coli, where cell death occurred at 10 and 30 minute exposures at concentrations of 0.19 and 0.14%, respectively. Due to the action of the disinfectant on the gram-positive bacteria S. aureus, cell death came at these exposures at concentrations 0.27 and 0.19%, respectively. The least susceptible to the action of the disinfectant were vegetative forms of B. subtilis, the death of these bacteria came at concentrations 0.37 and 0.27% at 10 and 30 minutes of exposure, respectively. Effects of disinfectant was over 5.38 and 2.74 times higher on microorganisms of E. coli and S. aureus, respectively, of the decontamination effect of phenol, and in the presence of protein, the activity of the disinfectant is reduced by 6.45 times. The efficiency of the disinfectant for the strains of microorganisms at the disinfestation of the test-object surfaces is determined. It has been established that for test cultures of E. coli and S. aureus 0.05–0.3% of disinfectant concentration is ineffective, and 0.5% its disinfectant concentration for disinfection of hard surfaces in preventive disinfection of premises for animals is insufficient for exposure in 120 minutes. For spore form B. subtilis 1.0% disinfectant concentration was ineffective. 1.0% concentration is effective for aseptic cleaning: slaughterhouses, processing shops, laboratory facilities, means of transporting products of animal origin, soaking clothes before washing, and also for treating surfaces of wood, metal and glazed tile at exposures of 60 minutes and more, and when spore forms of microorganisms, the working concentration of insoluble in disinfection of objects subject to veterinary control should be 3% and above. It has been proved that the disinfectant «GK-10» is effective at a concentration of 0.5% at exposure for 180 minutes and at a concentration of 1.0% at an exposure of 120 minutes for the sanation and preventive disinfection of premises for animals.

scholarly journals Бактерицидні та дезінфікуючі властивості деззасобу «Алкісепт–100»

2017 ◽  
Vol 19 (73) ◽  
pp. 61-65
Author(s):  
O.L. Tishyn ◽  
G.T. Kopijchuk ◽  
R.V. Khomiak ◽  
O.V. Khyrivskyy ◽  
M.M. Danko
Keyword(s):  
Cell Death ◽  
Production Environment ◽  
Protein Activity ◽  
Vegetative Cells ◽  
Spore Form ◽  
E Coli ◽  
Organic Impurities ◽  
Working Solution ◽  
Didecyldimethylammonium Chloride ◽  
Test Cultures

The results of research bactericidal properties of new domestic disinfectant «Alkisept–100» that based on a glutaraldehyde, didecyldimethylammonium chloride, alkyldimethylbenzylammonium chloride and aminotrismethylenephosphonic acid. Was found bactericidal dilution (BD), bactericidal concentration (BC), phenol coefficient (FC) and protein index (PI). Most susceptible to the effect of disinfectant was test-cultures of P. vulgaris, where cell death came on the 10 and 30 minutes exposures at concentrations of 0.26 and 0.18%, respectively. The action of disinfection preparation for gram-positive bacteria S. aureus, vegetative cells of B. subtilis vegetative cells and cultures of S. typhimurium test-cell death drew under these conditions at concentrations of 0.72 and 0.52%, respectively. Gram-negative bacteria E. coli were more susceptible to disinfectant and cell death drew for these exposures at concentrations of 0.52 and 0.37 %, respectively. BD of this disinfectat were 1.96 and 1.4 higher times for E. coli and S. aureus, respectively, from BD phenol, and in the presence of protein activity investigated disinfection preparation is reduced to 2.74 times. The efficacy of disinfection preparation of microorganisms on surfaces in the decontamination of test objects. It is established that test cultures of E. coli and S. aureus (without organic impurities) at 0.1% concentration of disinfectant is ineffective. For spore form of B. subtilis disinfection preparation concentration at 4.0%. Is ineffective At the 1.0–1.5% and higher concentrations is effective for surface treatment of wood, metal and tile that are contaminated with organic substances and objects subject to veterinary controls, and for elimination spore forms of microorganisms at its working concentration of desinfectant should be 5.0% or higher, the exposure in 120 minutes or more. Proved that the disinfectant «Alkisept–100» in a production environment at a concentration of 1.0% by wet surface irrigation facilities for animals and working solution 0.25–0.3 liters per 1 square meter with an exposure of 2 hours, showing high disinfectant properties.

scholarly journals Effect of Satureja montana essential oil on the bactericidal activity of broiler chickens blood serum

2020 ◽  
Vol 224 ◽  
pp. 04023
Author(s):  
V Pashtetsky ◽  
P Ostapchuk ◽  
O Postnikova ◽  
D Zubochenko ◽  
T Kuevda
Keyword(s):  
Essential Oil ◽  
Blood Serum ◽  
Broiler Chickens ◽  
Antimicrobial Effect ◽  
Feed Additive ◽  
Bacterial Biofilms ◽  
E Coli ◽  
Test Culture ◽  
Bactericidal Properties ◽  
Satureja Montana

Essential oils as a feed additive in animal and poultry nutrition should preserve the bactericidal properties of blood and suppress the formation of bacterial biofilms. The study of bacterial biofilms and bacterial activity of blood serum in broiler chickens against the background of the addition of essential oil of Satureja montana to a diet was discussed in the article. Blood serum of broilers that received essential oil with feed suppressed the growth of the bacterial biofilms at S. aureus test culture by 60-72%. It serum had a weaker effect on the bacterial biofilms at E. coli test culture; it reduced the density by 23.5%. This effect began only after the 10th day of inclusions of essential oil. The blood serum of chickens that received essential oil in the form of aqueous solutions had a weak antimicrobial effect against growing the bacterial biofilms at S. aureus test culture; the density was 10% lower than that of the control. The growth of the bacterial biofilms of E. coli test culture was not inhibited.

Antagonistic activity of Bacillus subtilis strains isolated from various sources

2018 ◽  
Vol 8 (2) ◽  
pp. 354-364
Author(s):  
A. N. Irkitova ◽  
A. V. Grebenshchikova ◽  
A. V. Matsyura
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Wild Strain ◽  
Fish Farming ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
E Coli ◽  
Long Period ◽  
Test Culture ◽  
Agar Media

<p>An important link in solving the problem of healthy food is the intensification of the livestock, poultry and fish farming, which is possible only in the adoption and rigorous implementation of the concept of rational feeding of animals. In the implementation of this concept required is the application of probiotic preparations. Currently, there is an increased interest in spore probiotics. In many ways, this can be explained by the fact that they use no vegetative forms of the bacilli and their spores. This property provides spore probiotics a number of advantages: they are not whimsical, easily could be selected, cultivated, and dried. Moreover, they are resistant to various factors and could remain viable during a long period. One of the most famous spore microorganisms, which are widely used in agriculture, is <em>Bacillus subtilis</em>. Among the requirements imposed to probiotic microorganisms is mandatory – antagonistic activity to pathogenic and conditional-pathogenic microflora. The article presents the results of the analysis of antagonistic activity of collection strains of <em>B. subtilis</em>, and strains isolated from commercial preparations. We studied the antagonistic activity on agar and liquid nutrient medias to trigger different antagonism mechanisms of <em>B. subtilis</em>. On agar media, we applied three diffusion methods: perpendicular bands, agar blocks, agar wells. We also applied the method of co-incubating the test culture (<em>Escherichia coli</em>) and the antagonist (or its supernatant) in the nutrient broth. Our results demonstrated that all our explored strains of <em>B. subtilis</em> have antimicrobial activity against a wild strain of <em>E. coli</em>, but to varying degrees. We identified strains of <em>B. subtilis</em> with the highest antagonistic effect that can be recommended for inclusion in microbial preparations for agriculture.</p><p><em><br /></em><em></em></p>

Synthesis of Chalcones and Nucleosides Incorporating [1, 3, 4]Oxadiazolenone Core and Evaluation of their Antifungal and Antibacterial Activities

2020 ◽  
Vol 15 (6) ◽  
pp. 665-679
Author(s):  
Alok K. Srivastava ◽  
Lokesh K. Pandey
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Good Antibacterial Activity

Background: [1, 3, 4]oxadiazolenone core containing chalcones and nucleosides were synthesized by Claisen-Schmidt condensation of a variety of benzaldehyde derivatives, obtained from oxidation of substituted 5-(3/6 substituted-4-Methylphenyl)-1, 3, 4-oxadiazole-2(3H)-one and various substituted acetophenone. The resultant chalcones were coupled with penta-O-acetylglucopyranose followed by deacetylation to get [1, 3, 4] oxadiazolenone core containing chalcones and nucleosides. Various analytical techniques viz IR, NMR, LC-MS and elemental analysis were used to confirm the structure of the synthesised compounds.The compounds were targeted against Bacillus subtilis, Staphylococcus aureus and Escherichia coli for antibacterial activity and Aspergillus flavus, Aspergillus niger and Fusarium oxysporum for antifungal activity. Methods: A mixture of Acid hydrazides (3.0 mmol) and N, Nʹ- carbonyl diimidazole (3.3 mmol) in 15 mL of dioxane was refluxed to afford substituted [1, 3, 4]-oxadiazole-2(3H)-one. The resulted [1, 3, 4]- oxadiazole-2(3H)-one (1.42 mmol) was oxidized with Chromyl chloride (1.5 mL) in 20 mL of carbon tetra chloride and condensed with acetophenones (1.42 mmol) to get chalcones 4. The equimolar ratio of obtained chalcones 4 and β -D-1,2,3,4,6- penta-O-acetylglucopyranose in presence of iodine was refluxed to get nucleosides 5. The [1, 3, 4] oxadiazolenone core containing chalcones 4 and nucleosides 5 were tested to determined minimum inhibitory concentration (MIC) value with the experimental procedure of Benson using disc-diffusion method. All compounds were tested at concentration of 5 mg/mL, 2.5 mg/mL, 1.25 mg/mL, 0.62 mg/mL, 0.31 mg/mL and 0.15 mg/mL for antifungal activity against three strains of pathogenic fungi Aspergillus flavus (A. flavus), Aspergillus niger (A. niger) and Fusarium oxysporum (F. oxysporum) and for antibacterial activity against Gram-negative bacterium: Escherichia coli (E. coli), and two Gram-positive bacteria: Staphylococcus aureus (S. aureus) and Bacillus subtilis(B. subtilis). Result: The chalcones 4 and nucleosides 5 were screened for antibacterial activity against E. coli, S. aureus and B. subtilis whereas antifungal activity against A. flavus, A. niger and F. oxysporum. Compounds 4a-t showed good antibacterial activity whereas compounds 5a-t containing glucose moiety showed better activity against fungi. The glucose moiety of compounds 5 helps to enter into the cell wall of fungi and control the cell growth. Conclusion: Chalcones 4 and nucleosides 5 incorporating [1, 3, 4] oxadiazolenone core were synthesized and characterized by various spectral techniques and elemental analysis. These compounds were evaluated for their antifungal activity against three fungi; viz. A. flavus, A. niger and F. oxysporum. In addition to this, synthesized compounds were evaluated for their antibacterial activity against gram negative bacteria E. Coli and gram positive bacteria S. aureus, B. subtilis. Compounds 4a-t showed good antibacterial activity whereas 5a-t showed better activity against fungi.

scholarly journals A novel bis(pyrazolyl)methane compound as a potential agent against Gram-positive bacteria

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Pedro Seguí ◽  
John J. Aguilera-Correa ◽  
Elena Domínguez-Jurado ◽  
Christian M. Sánchez-López ◽  
Ramón Pérez-Tanoira ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Effect ◽  
Eukaryotic Cell ◽  
Liver Carcinoma ◽  
Gram Positive ◽  
Minimal Inhibitory Concentrations ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Therapeutic Compounds ◽  
Toxic Concentrations

AbstractThis study was designed to propose alternative therapeutic compounds to fight against bacterial pathogens. Thus, a library of nitrogen-based compounds bis(triazolyl)methane (1T–7T) and bis(pyrazolyl)methane (1P–11P) was synthesised following previously reported methodologies and their antibacterial activity was tested using the collection strains of Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa. Moreover, the novel compound 2P was fully characterized by IR, UV–Vis and NMR spectroscopy. To evaluate antibacterial activity, minimal inhibitory concentrations (MICs), minimal bactericidal concentrations (MBCs), minimum biofilm inhibitory concentrations (MBICs), and minimum biofilm eradication concentrations (MBECs) assays were carried out at different concentrations (2–2000 µg/mL). The MTT assay and Resazurin viability assays were performed in both human liver carcinoma HepG2 and human colorectal adenocarcinoma Caco-2 cell lines at 48 h. Of all the synthesised compounds, 2P had an inhibitory effect on Gram-positive strains, especially against S. aureus. The MIC and MBC of 2P were 62.5 and 2000 µg/mL against S. aureus, and 250 and 2000 µg/mL against E. faecalis, respectively. However, these values were > 2000 µg/mL against E. coli and P. aeruginosa. In addition, the MBICs and MBECs of 2P against S. aureus were 125 and > 2000 µg/mL, respectively, whereas these values were > 2000 µg/mL against E. faecalis, E. coli, and P. aeruginosa. On the other hand, concentrations up to 250 µg/mL of 2P were non-toxic doses for eukaryotic cell cultures. Thus, according to the obtained results, the 2P nitrogen-based compound showed a promising anti-Gram-positive effect (especially against S. aureus) both on planktonic state and biofilm, at non-toxic concentrations.

scholarly journals Evaluation of the Antimicrobial Activity of Some Components of the Essential Oils of Plants Used in the Traditional Medicine of the Tehuacán-Cuicatlán Valley, Puebla, México

Antibiotics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 295
Author(s):  
Sebastián Candelaria-Dueñas ◽  
Rocío Serrano-Parrales ◽  
Marisol Ávila-Romero ◽  
Samuel Meraz-Martínez ◽  
Julieta Orozco-Martínez ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Qualitative Evaluation ◽  
Bactericidal Effect ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Diffusion Technique ◽  
Agar Dilution

In Tehuacán-Cuicatlán valley (Mexico), studies have been carried out on the essential oils of medicinal plants with antimicrobial activity and it was found that they present compounds in common such as: α-pinene, β-pinene, carvacrol, eugenol, limonene, myrcene, ocimene, cineole, methyl salicylate, farnesene, and thymol. The goal of this study was to assess the antimicrobial activity of essential oils’ compounds. The qualitative evaluation was carried out by the Kirby Baüer agar diffusion technique in Gram-positive bacteria (11 strains), Gram-negative bacteria (18 strains), and yeasts (8 strains). For the determination of the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), the agar dilution method was used. All the evaluated compounds presented antimicrobial activity. The compounds eugenol and carvacrol showed the largest inhibition zones. Regarding yeasts, the compounds ocimene, cineole, and farnesene did not show any activity. The compounds eugenol, carvacrol, and thymol presented the lowest MIC; bactericidal effect was observed at MIC level for S. aureus 75MR, E. coli 128 MR, and C albicans CUSI, for different compounds, eugenol, carvacrol, and thymol. Finally, this study shows that the essential oils of plants used by the population of Tehuacán-Cuicatlán valley share compounds and some of them have antibacterial and fungicidal activity.

Preparation of bioactive and antibacterial PMMA-based bone cement by modification with quaternary ammonium and alkoxysilane

2021 ◽  
pp. 088532822110044
Author(s):  
Haiyang Wang ◽  
Toshinari Maeda ◽  
Toshiki Miyazaki
Keyword(s):  
Antibacterial Activity ◽  
Methyl Methacrylate ◽  
Bone Cement ◽  
Setting Time ◽  
Antibacterial Properties ◽  
The Body ◽  
Apatite Formation ◽  
Gram Positive ◽  
E Coli ◽  
Gram Positive Bacteria

Bone cement based on poly(methyl methacrylate) (PMMA) powder and methyl methacrylate (MMA) liquid is a very popular biomaterial used for the fixation of artificial joints. However, there is a risk of this cement loosening from bone because of a lack of bone-bonding bioactivity. Apatite formation in the body environment is a prerequisite for cement bioactivity. Additionally, suppression of infection during implantation is required for bone cements to be successfully introduced into the human body. In this study, we modified PMMA cement with γ-methacryloxypropyltrimetoxysilane and calcium acetate to introduce bioactive properties and 2-( tert-butylamino)ethyl methacrylate (TBAEMA) to provide antibacterial properties. The long-term antibacterial activity is attributed to the copolymerization of TBAEMA and MMA. As the TBAEMA content increased, the setting time increased and the compressive strength decreased. After soaking in simulated body fluid, an apatite layer was detected within 7 days, irrespective of the TBAEMA content. The cement showed better antibacterial activity against Gram-negative E. Coli than Gram-positive bacteria; however, of the Gram-positive bacteria investigated, B. subtilis was more susceptible than S. aureus.

scholarly journals Poly(Vinylamine) Derived N-Doped C-Dots with Antimicrobial and Antibiofilm Activities

2021 ◽  
Vol 7 (2) ◽  
pp. 40
Author(s):  
Semiha Duygu Sutekin ◽  
Mehtap Sahiner ◽  
Selin Sagbas Suner ◽  
Sahin Demirci ◽  
Olgun Güven ◽  
...  
Keyword(s):  
Blood Clotting ◽  
Minimum Bactericidal Concentration ◽  
Blood Compatibility ◽  
Weight Ratio ◽  
Gram Negative Bacteria ◽  
Biofilm Inhibition ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Doped Carbon Dots ◽  
Nitrogen Doped Carbon

Nitrogen-doped carbon dots (N-doped C-dots) was synthesized by using poly(vinyl amine) (PVAm) as a nitrogen source and citric acid (CA) as a carbon source via the hydrothermal method. Various weight ratios of CA and PVAm (CA:PVAm) were used to synthesize N-doped C-dots. The N-doped C-dots revealed emission at 440 nm with excitation at 360 nm and were found to increase the fluorescence intensity with an increase in the amount of PVAm. The blood compatibility studies revealed no significant hemolysis for N-doped C-dots that were prepared at different ratios of CA:PVAm for up to 500 μg/mL concentration with the hemolysis ratio of 1.96% and the minimum blood clotting index of 88.9%. N-doped C-dots were found to be more effective against Gram-positive bacteria than Gram-negative bacteria, with the highest potency on Bacillus subtilis (B. subtilis). The increase in the weight ratio of PVAm in feed during C-dots preparation from 1 to 3 leads to a decrease of the minimum bactericidal concentration (MBC) value from 6.25 to 0.75 mg/mL for B. subtilis. Antibiofilm ability of N-doped C-dots prepared by 1:3 ratio of CA:PVAm was found to reduce %biofilm inhibition and eradication- by more than half, at 0.78 mg/mL for E. coli and B. subtilis generated biofilms and almost destroyed at 25 mg/mL concentrations.

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