scholarly journals ЗМІНИ БІЛКОВИХ ПОКАЗНИКІВ КРОВІ КОРОПА ЗА ВИКОРИСТАННЯ КОМПЛЕКСУ СИМБІОНТНИХ МІКРООРГАНІЗМІВ

2016 ◽  
Vol 18 (3(70)) ◽  
pp. 181-184
Author(s):  
T. Mazur ◽  
I. Garkusha
Keyword(s):  
Lactobacillus Acidophilus ◽  
Total Protein ◽  
Fish Farming ◽  
Antagonistic Effect ◽  
The Body ◽  
Blood Protein ◽  
Physiological Status ◽  
Blood Levels ◽  
Accurate Information ◽  
The Impact

In modern conditions the intensification of cultivation and fish farming industrial fish farming today is based on the principles of technological pipeline. One way of improving the technology of cultivation and breeding of fish and maintain normal physiological status is the use of probiotic microorganisms. Fruits included in their composition can produce different active substances disposed of harmful metabolic products provide an antagonistic effect on pathogens. It is known that the use of probiotics affect the cellular and biochemical composition of blood, including the performance level of protein in the blood. The purpose of these studies was to determine the impact of complex probiotic microorganism Bacillus subtillis and Lactobacillus acidophilus compared with the use of these monocultures of microorganisms on the blood protein fractions of common carp. Through monitoring of total protein in serum may receive the most accurate information about the immune status carp. It was found that the inclusion in the diet of carp probiotic complex consisting of Bacillus subtilis and Lactobacillus acidophilus vplyvayea more positive on blood levels of total protein and its fractions in the serum of carp than using the latest in a monoculture. Besides data indicate intensification of metabolic processes in the body of the fish. However, the increase in γ–globulins shows a positive effect probiotynoho complex on humoral immunity factor carp. 

scholarly journals Absorption of Polyunsaturated Fatty Acid (PUFA) Is Related to IgG Blood Levels of Neonatal Pigs during the First 48 Hours Postpartum

2020 ◽  
Vol 2020 ◽  
pp. 1-8 ◽  
Author(s):  
Kateryna Pierzynowska ◽  
Jarosław Woliński ◽  
Björn Weström ◽  
Radosław Jazwiec ◽  
Halyna Shmigel ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Infant Formula ◽  
Polyunsaturated Fatty Acid ◽  
Body Weight Gain ◽  
Dietary Guidelines ◽  
The Body ◽  
Blood Levels ◽  
Human Infants ◽  
Neonatal Pigs ◽  
The Impact

The current study is aimed at highlighting the impact of enterally or parenterally applied immunoglobulins (Igs) on polyunsaturated fatty acid (PUFA) absorption in newborn pigs. Piglets were chosen as the appropriate model since they are born agammaglobulinemic and any effects of Ig addition can thus be easily monitored. Twenty-one, new born piglets were used in the study. Plasma levels of PUFAs, ARA, DHA, and EPA dropped (similarly to that seen in human infants) by between 40 and 50% in newborn, unsuckled piglets fed an infant formula for 48 h. However, piglets fed the same infant formula but supplied with immunoglobulins (Igs) either orally, by feeding piglets with swine or bovine colostrum, or intravenously, by i.u.a. (intraumbilical artery) infusion of swine or human Ig preparations or swine serum, demonstrated improved growth and PUFA levels similar to those observed at birth. The significant positive correlation was found between the body weight gain, as well as levels of ARA and EPA, and plasma immunoglobulins concentration. These results indicate the importance of the presence of Ig in the blood for appropriate absorption of dietary PUFAs and probably other nutrients in newborn piglets. This may have an impact on the dietary guidelines for human neonates, especially those born prematurely with low plasma Ig levels, since PUFAs are important factors for brain development in early life.

scholarly journals Impact des extraits éthyliques des feuilles de Hexalobus Monopétalus sur lemétabolisme protéiques des rats Wistar

2018 ◽  
Vol 6 (01) ◽  
Author(s):  
Akotegnon Rodrigue ◽  
Michodjehoun Loetitia ◽  
Behanzin Justin ◽  
Houeze Elisabeth ◽  
Sezan Alphonse
Keyword(s):  
Uric Acid ◽  
Total Protein ◽  
Wistar Rats ◽  
Polyphenolic Compounds ◽  
Blood Levels ◽  
Phytochemical Screening ◽  
Duration Of Treatment ◽  
Anthracene Derivatives ◽  
The Impact

The aim of this study was to evaluate the impact of the ethyl extract of dried leaves of Hexalobus monopetalus on protein metabolism in Wistar rats. After a phytochemical screening of extracts, total protein, urea, uric acid and creatinine were measured spectrophotometrically at six (06) sets of three (03) Wistar rats each. Batches 2 to 6 were given with orally administered respectively the daily doses of: 0.25 mg / kg, 0.5mg / kg, 1mg / kg, 1.5mg / kg and 2.5mg kg body weight of the extracts (for 14 days). The lot1 witness received distilled water instead of the extract. The results of the phytochemical screening revealed the presence polyphenolic compounds (gallic tannins, catechin tannin or condensed, anthocyanins, and leuco anthocyanins), flavonoids, mucilages reducing compounds, alkaloids, certain anthracene derivatives ( free anthracene, the O-glycosides), Steroids, and quinone derivatives. It was observed a significant increase (p ˂ 0.05) blood levels of total protein and uric acid and a significant (p ˂ 0.05) blood urea and creatinine levels in treated plots to extracts. These observed variations did not depend on the administered dose of the extracts but were depending on the duration of treatment; they were outstanding from the 7th day of treatment. The long term use of ethyl extract of dried leaves of Hexalobus monopetalus could compromise the liver function and the kidneys and may inhibit apoptosis in Wistar rats.

Trace Elements Burden in Geriatric Hemodialysis Patients: A Prospective Multicenter Collaborative Study

1997 ◽  
Vol 20 (10) ◽  
pp. 553-556 ◽  
Author(s):  
K.Y. Hung ◽  
C.Y. Ho ◽  
Y.M. Kuo ◽  
S.H. Lee ◽  
S.J. Hseih ◽  
...  
Keyword(s):  
Aging Process ◽  
Collaborative Study ◽  
Atomic Absorption Spectrophotometry ◽  
The Body ◽  
Blood Levels ◽  
Blood Concentrations ◽  
Normal Limits ◽  
Starting Point ◽  
Cellulose Membranes ◽  
The Impact

Introduction Hemodialysis therapy and the aging process bave been reported to interfere with the trace element (TE) status in the body. This multicenter collaborative study on blood levels of TE in geriatric hemodialysis (HD) patients was carried out with an aim to clarify the impact of the aging process and HD therapy on the TE status in such patients. Methods One hundred and fifty-one HD patients (65 male, 86 female), all aged over 60 (68.2±3.86 y), and 112 elderly controls (58 male, 54 female) with a mean age of 67.5±3.03 were enrolled. All patients underwent standard HD for at least 6 months. The artificial kidneys used were hollow-fiber dialyzers of cellulose membranes. Water used for HD was prepared by reverse osmosis. Blood was collected in the morning prior to dialysis and after fasting. Atomic absorption spectrophotometry was applied to measure blood levels of Pb, Cd and Hg as well as plasma levels of Cu, Zn and Al. Data are presented as mean ± SEM. Student's t-test and linear regression were applied for statistics. Results Our geriatric chronic HD patients showed a marked elevation of blood Al and decreased plasma Zn concentrations. The blood levels of Cu, Cd, Pb and Hg were within normal limits. The blood concentrations of Al, Cu and Pb were significantly higher in HD patients than in the normal elderly controls, whereas Cd and Hg were similar in both groups, and Zn were lower in HD patients. Plasma Zn value decreased as dialysis duration increased, however there was a reversely linear correlation between plasma Zn and age. Conclusions This data reported can be regarded as a starting point and may serve as an insight to further studies on TE imbalances in geriatric and chronic HD populations.

scholarly journals Strategy for trout feeding with Aller aqua feeds

2018 ◽  
Vol 20 (84) ◽  
Author(s):  
I. Y. Bobel ◽  
J. I. Pivtorak
Keyword(s):  
Growth Rate ◽  
Mouth Opening ◽  
Fish Farming ◽  
Maximum Growth ◽  
The Body ◽  
Feeding Strategies ◽  
Natural Food ◽  
Young Fish ◽  
Nitrogen And Phosphorus ◽  
The Impact

Due to the fact that the cost of forage for trout is up to 60% of all costs for its cultivation, the problem of rational use and saving has a special place. In the planned production the selection of the appropriate feed and feeding strategies are very important. Two extremely important coefficients of the indicator should be taken into consideration: coefficient of growth rate and fodder coefficient. The maximum growth rate is not correlates with the best fodder coefficient. All Allerg aqua production feeds are made in the form of ellipsoid granules. According to Aller aqua, the ellipsoid shape of the granules can significantly improve feed and, consequently, reduce its loss. When immersed in water, granular feeds at the expense of the ellipsoid form carry chaotic movements that attract fish to their capture. In addition, the granules of the ellipsoid form in the production process are better saturated with fat. Feeding trout it is necessary to take into account that the size of the granules for young fish in the length of 4–20 cm should be from 2.2 to 2.6% of the body length (according to Smith), which corresponds to the mouth opening size and the distance between the gill stamens in rainbow trout. The onset of feeding in time leads to the proper formation of the esophagus, which allows further intensive feeding. Provision of high quality fodder is one of the most important conditions for successful industrial fish farming. In conditions where the fish is devoid of natural food, the metabolism is controlled by humans and depends on the balance, quality and quantity of feed supplied to the fish. That is the reason of great opportunities for increasing the rate of growth of fish, with minimal expenses for mixed fodder, reducing the mortality of young fish, improving the quality of producers, and in general improving the efficiency of all fish farming processes. In order to determine with great accuracy the norms of feeding, it is necessary to carry out the control of weighing of fish. The size of the sample depends on the size of the pool and the amount of fish. The sampling should be from 50 to 100% of the general population in small containers and at least 1% in large pools. Fish is best fed often, in small portions. The smaller the fish, the more it needs to be fed. If necessary, it is needed to use special therapeutic granulated food or a mixture of therapeutic ingredients to add to the wet granules. Fish should be fed every day, since breaks lead to a decrease in its mass. It is necessary to create optimal conditions for cultivation and to carry out constant monitoring of water temperature and its fluctuations, oxygen content in water, pH, atmospheric pressure, etc. The impact of feed on the environment is also closely related to the value of the feed factor. The main products of fish metabolism that are isolated in aqueous medium, it is nitrogen and phosphorus. The Aller Aqua Feeding Program for trout includes the following: The amount of feed fed with trout depends on the temperature and size of the fish. These factors affect the amount of ration feeding.

STUDY OF THE EFFECT OF FEED ADDITIVE OF SODIUM HUMATE FOR STERLET BY THE CAGE METHOD OF GROWING

2021 ◽  
Vol 12 (4) ◽  
Author(s):  
N. V. Polishchuk ◽  
B. Yu. Kovalenko ◽  
N. I. Vovk ◽  
I. S. Kononenko ◽  
V. O. Kovalenko
Keyword(s):  
Growth Rate ◽  
Age Groups ◽  
Fish Farming ◽  
Feed Additives ◽  
The Body ◽  
Biologically Active ◽  
Feed Additive ◽  
Fish Feed ◽  
Sodium Humate ◽  
The Impact

The article presents the results of an experiment to assess the effect of feed additive of humates on the growth rate and survival of different age groups of sterlet (Acipenser ruthenus L., 1758) and the conversion of fish feed for keeping in aquaculture. Based on the analysis of sources of scientific and technical information, it was found that humic drugs have long been used in crop production as growth stimulants and microfertilizers, and in animal husbandry - to normalize metabolism, treatment of digestive diseases and general stimulating effect on the body as a whole. Analysis of data on the use of humates in aquaculture indicated the presence of a positive effect of these compounds on individual cultivation objects. The small amount of information on the use of humates in fish farming has led to the conclusion that this direction of fish farming intensification is still insufficiently studied, and that studies of the impact of humic substances on aquaculture objects are not comprehensive and mainly concern the assessment of individual effects of biologically active action of the compounds on fish. The available information gave grounds to speculate about the prospects for the use of humates in sturgeon farming. To test this assumption, a series of experiments was conducted on the feeding of sterlet feed with the addition of sodium humate. The research was conducted in 2018, 2020 and 2021, in the production conditions of the cage fishery, located on the Kaniv Reservoir. The effect of different concentrations of sodium humate feed additives on one- and two year old sterlet was evaluated. It was found that the addition of sodium humate to fish feed in concentrations of 60, 100, 120 and 200 mg / kg of feed did not significantly affect the growth rate of sterlet. Thus, the advantage of experimental variants over the control for this indicator was insignificant, in the range of 1.0-1.6%. The effect of sodium humate on the survival of this year sterlet, for their cultivation in gardens. At the same time, the best, by 6.7-17.4%, feed conversion with the addition of humates by one- and two-year-old sterlet was noted. According to the results of the experiments, the best result was obtained in the variant using a feed additive of humates at a concentration of 200 mg / kg of feed. It is considered promising to continue the study of the impact of feed additives of humates on the growth and survival of valuable aquaculture objects under different housing conditions, in particular in closed recirculation aquasystems (RAS), as well as finding the optimal concentration of these additives in feed for different species and age groups of fish.

ОСОБЕННОСТИ БЕЛКОВОГО ОБМЕНА У БЫКОВ-ПРОИЗВОДИТЕЛЕЙ РАЗНЫХ ВОЗРАСТОВ

2019 ◽  
Author(s):  
N.V. BOGOLYUBOVA ◽  
I.V. GUSEV ◽  
R.A. RYKOV ◽  
N.A. KOMBAROVA
Keyword(s):  
Reference Values ◽  
Total Protein ◽  
Metabolic Profile ◽  
The Body ◽  
Farm Animals ◽  
Blood Levels ◽  
Serum Samples ◽  
Health And Nutrition ◽  
Relationship Of ◽  
The Relationship

Для разработки референтных значений биохимических показателей, характеризующих нормальное течение обменных процессов, исследованы пробы сыворотки крови быков-производителей голштинской породы (n=102), разделенных на 4 группы по возрасту и содержащихся в условиях племпредприятия Московской области. В сыворотке крови определяли концентрацию общего белка, альбумина, мочевины, креатинина, активность аланинаминотрансферазы (АЛТ), аспартатаминотрансферазы (АСТ). Установлено, что с возрастом быков достоверно увеличивается содержание в крови общего белка. У животных 2—4 лет этот показатель увеличился на 11% (P<0,01), 4—6 лет — на 19,1% (P<0,01), более 6 лет — на 21,5% (P<0,001) по сравнению с быками до 2-летнего возраста. Это связано с повышением глобулиновой фракции. С возрастом быков в связи с увеличением их живой массы отмечалось повышение концентрации креатинина в крови, а также мочевины. У производителей 4—6-летнего возраста содержание мочевины в крови увеличивалось на 47,9% (P<0,001) по сравнению с быками до 2 лет. Повышение активности АСТ у быков-производителей с возрастом можно связать с повышенной нагрузкой на печень и сердечно-сосудистую систему в связи с их производственным использованием. Полученные данные о метаболическом профиле производителей разных возрастов помогут на практике проводить мониторинг и корректировку состояния животных, а также в разработке эталонных значений для оценки здоровья и уровня питания.Questions of the relationship of indicators of protein metabolism in the body of bulls with age can serve as accumulative material for the development of reference values of biochemical indicators characterizing the normal course of metabolic processes. The serum samples of the Holstein breeding bulls (n=102), divided into 4 groups by age and contained in the conditions of the “Head Center for the Reproduction of Farm Animals”, were investigated. The following parameters were determined in blood serum: concentration of total protein, albumin, urea, creatinine, activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST). As a result of research, it has been established that with age, bulls reliably increase blood levels of total protein. In the 2—4 year old bulls, this indicator increased by 11% (P<0.01), 4-6 years old — by 19.1% (P<0.01), and over 6 years old — by 21.5% (P<0,001) compared with animals under 2 years. This was associated with an increase in the globulin fraction. With increasing age of the bulls, an increase in the concentration of creatinine in the blood was observed, which is associated with an increase in body weight of animals. Noted the increase in urea in the blood of the bulls with age. Thus, in the blood of bulls of 4—6 years of age, this indicator increased by 47.9% (P<0.001) as compared with analogues up to 2 years. An increase in AST activity in bulls with age can be associated with an increased load on the liver and the cardiovascular system due to their production use. The obtained data on the metabolic profile of the bulls of different ages will help in practice to monitor and adjust the condition of the animals and to develop reference values for assessing health and nutrition levels. Key

scholarly journals EFFECT OF GOLD AND NICKEL NANOPARTICLES ON IMMUNE PROTEINS OF TOXOPLASMOSIS PATIENTS

2017 ◽  
Vol 10 (10) ◽  
pp. 162
Author(s):  
Sura A Abdulsattar ◽  
Abdullah H Drywall
Keyword(s):  
Gold Nanoparticles ◽  
Immune System ◽  
Total Protein ◽  
Nickel Nanoparticles ◽  
Specific Activity ◽  
Immune Defense ◽  
The Body ◽  
Medical Applications ◽  
Dual Effect ◽  
The Impact

  Objective: An interaction between a nanoparticle and the immune system is considered desirable when it may lead to various beneficial medical applications such as vaccines, delivery of drugs, antigens or therapeutics for inflammatory and autoimmune disorders. This study aims to evaluate the impact of some immune proteins by the presence of gold and nickel nanoparticles in sera of toxoplasmosis patients.Methods: A total of 20 patients women with toxoplasmosis aged (20-40 year) attending Central Health Laboratory in Baghdad city for the period (November 2016 to February 2017) and 20 age-matched of women healthy as a control were included in this study.Results: A non-significant increase in the activity and specific activity of protease in the sera of women infected with toxoplasmosis were observed in comparison to their values in the healthy (p˃0.05). While a non-significant decrease in total protein values was reported. The results of effect of nanoparticles showed a decrease in the level of the enzyme in the presence of nanoparticles by 41.3%, while the results showed that inhibition of nickel nanoparticles was greater and 43.8%. The results indicated that IgA, IgG, and IgM levels were decreased in the presence of gold nanoparticles. In the same context, both IgA and IgG were shown to be inhibitory in the presence of nickel nanoparticles while the results showed activation of the IgM level with nickel nanoparticles.Conclusion: We conclude from this study that other studies are required to confirm our results and therefore support the possibility of using nickel nanoparticles as a support for the treatment of toxoplasmosis through its dual effect first by reducing the protease, which is essential for survival of the parasite and second by activating IgM level, which enhances the immune defense of the body against the parasite.

scholarly journals Вплив препарату «Трифузол» на функціональний стан печінки у курчат за еймеріозної інвазії

2017 ◽  
Vol 19 (77) ◽  
pp. 76-79
Author(s):  
M. Kryshtalska ◽  
V. Hunchak ◽  
B. Gutyj
Keyword(s):  
Liver Function ◽  
Blood Serum ◽  
Total Protein ◽  
Physiological Role ◽  
The Body ◽  
Blood Proteins ◽  
Physiological Variables ◽  
Blood Biochemical ◽  
Vital Functions ◽  
The Impact

In the process of vital functions chicken blood proteins play an important physiological role in the complex processes of metabolism. Changes in blood biochemical composition of chickens according to experimental invasions reflect the development of pathological and immune processes and point on the stress of the body, which makes it possible objectively evaluate the impact of various factors on the organism of infested chickens. We have found that chickens infected eymeriozic invasion in blood serum was a low level of total protein and especially its albumin fraction. This indicates inhibition of protein synthesized liver function. In return the content of globulin in blood serum of sick chickens was 12% higher. On the background of the general hypoproteinemia was found a significant disparity between albumin and globulins in blood serum of sick chickens, as indicated by a decrease in the A/T ratio. After applying for the treatment of chickens affected eymeriozic invasion, drug «Brovafom», due to its antiprotozoic action the influence of toxins eliminates on the liver and intestinal mucosa. However, the protein synthesized function of liver at 28 day of the experiment in experimental chickens has not been completely restored. In applying the drug «Trifuzol» to chickens from the research group D3, for eymeriozic invasion we have found the level increase in total protein and albumin fractions, and also level lowering of globulin in serum of chickens throughout the experiment. At the 21st and 28th day of research in chickens from the experimental group we observe the indices normalization of protein synthesized function of the liver. Within physiological variables was also A/T ratio. Combined use of Brovafom and Trifuzol to experimental chickens for eymeriozic invasion, contributed to better normalization in protein synthesized of liver function, than the use of Brovafom.

scholarly journals Influence of the liposomal preparation Butaintervite on protein synthesis function in the livers of rats under the influence of carbon tetrachloride poisoning

2016 ◽  
Vol 7 (2) ◽  
pp. 123-126
Author(s):  
M. I. Hariv ◽  
B. V. Gutyj
Keyword(s):  
Oxidative Stress ◽  
Protein Synthesis ◽  
Carbon Tetrachloride ◽  
Total Protein ◽  
The Body ◽  
Control Group ◽  
Blood Levels ◽  
Carbon Tetrachloride Poisoning ◽  
Liposomal Preparation ◽  
Experimental Group

This article presents the results of research into the influence of the complex liposomal preparation Butaintervit on protein synthesis function in the livers of rats under the influence of carbon tetrachloride poisoning. Intramuscular injection of carbon tetrachloride into rats at a dose of 0.25 ml per100 gof body weight causes antigenic load on the body and leads to disruption of protein synthesis function in the liver. This is shown by reduction in blood levels of total protein and its fractions. Thus, the level of albumin in the serum of rats under the conditions of oxidative stress was 70% lower than in clinically healthy animals. However, the level of total protein in the serum was only 10% lower. This is because, along with the decrease of albumin content in the serum, the levels of globulin protein fraction increased by 8.8%. This has led to albumin/globulin disparities in the serum of sick animals. As a result, the value of A/G coefficient was 0.28 ± 0.03, compared to 0.52 ±0.02 inclinically healthy rats. For the normalization of functional state of the liver under oxidative stress it is advisable to apply the liposomal preparation Butaintervite, which in its structure contains butafosfan, interferon, thistle and vitamins A, D and E. Under conditions of oxidative stress and under the action of the liposomal preparation in the rats from the second experimental group we have found significant increase in the levels of total protein and albumins and a decrease in serum globulin in the animals on the fifth and tenth days of the experiment. On the fourteenth day of the experiment under the conditions of oxidative stress and under the action of the liposomal preparation in the rats from the second experimental group the normalization of protein synthesis function in the liver was observed. The level of indicators of total protein, albumin, globulin and the coefficient of albumin/globulin compared with the control group of animals were within normal values.

scholarly journals The effect of dietary protein on reproduction in the mare. II. Growth of foals, body mass of mares and serum protein concentration of mares during the anovulatory, transitional and pregnant periods

1997 ◽  
Vol 68 (3) ◽  
Author(s):  
F.E. Van Niekerk ◽  
C.H. Van Niekerk
Keyword(s):  
Body Mass ◽  
Serum Protein ◽  
Total Protein ◽  
Protein Intake ◽  
National Research Council ◽  
The Body ◽  
Blood Protein ◽  
Total Serum Protein ◽  
Total Serum ◽  
Total Protein Intake

The effect of 4 different diets, in terms of protein quantity and quality, on total serum protein (TSP), albumin and globulin was investigated. Non-pregnant mares that were not lactating (n = 36), pregnant mares that had foaled (n = 24) and their foals (n = 24) were used in this study. Daily total protein intake had no effect on blood protein concentrations in the mares. Total protein intake and quality (available essential amino-acids) did affect the body mass of mares during lactation. When mares were fed the minimum recommended (National Research Council 1989) total daily protein, foal mass decreased by approximately 25 % at weaning compared to the foals whose dams were on a higher level of protein intake. The TSP concentrations of foals at birth were on average 10 g/ℓ lower than those of the mares. Albumin concentrations of foals during the first 60 days of life were on average 2-3 g/ℓ lower than those of the mares. Globulin concentrations of foals were approximately 5 g/ℓ lower than those of mares at weaning.

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