scholarly journals Influence of seasonal factors on carp fish immune reactivity

2019 ◽  
Vol 9 (3) ◽  
pp. 168-173
Author(s):  
O. P. Rudenko ◽  
R. P. Paranyak ◽  
N. A. Kovalchuk ◽  
L. P. Kit ◽  
N. I. Hranovich ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
Functional Activity ◽  
Abiotic Factors ◽  
Immune Reactivity ◽  
Receptor Density ◽  
Seasonal Factors ◽  
Different Seasons ◽  
Degree Of Differentiation ◽  
Carp Fish

The article presents data about influence of seasonal factors on immune reactivity in carp scaly, carp framey, and carp. We studied the blood that was taken from the heart of fish in different seasons: spring (May), summer (August) and autumn (October). The research was carried out at the Lviv department of the Institute of Fisheries of the National Academy of Agrarian Science, Velykyj Ljubin in three groups of two-year-old fish. The received data give reason to think that the number of T- and B-lymphocytes in the blood of carp fish and their functional activity to a large extent depends on the influence of seasonal factors (temperature of the medium, oxygen concentration, duration of light day). This is points to the probable increase in the number of T-lymphocytes (common, active, theophylline-sensitive and theophylline-resistant) and a decrease in B-lymphocytes in the blood of investigated fish in the summer and, especially in the autumn period of research. In this case, an increase in the functional activity of T-lymphocytes was revealed due to the redistribution of the receptor apparatus of the immunocompetent cells. In particular, a reduction in the number of blood of inactive T-lymphocytes in functionally and an increase in cells with low and medium receptor density. Regarding the degree of differentiation of B-lymphocytes, the decrease in their amount in the blood of the studied individuals occurred due to the increase of “zero” and low-conductivity EAS-RUL and reduction of the subpopulation with average receptor density. We suggested that the abiotic factors can modulate the immune response of carp fish.

scholarly journals Influence of immunomodulators of natural origin on cellular immunity indices in blood of broiler chicken under stress

2015 ◽  
Vol 6 (1) ◽  
pp. 36-39
Author(s):  
S. Grabovskyi ◽  
O. Grabovska
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
Functional Activity ◽  
Broiler Chicken ◽  
Alcohol Solution ◽  
T And B Lymphocytes ◽  
Animal Origin ◽  
Natural Origin ◽  
Cell Immunity ◽  
Chicken Blood

The paper deals with researching of T- and B-lymphocytes relative quantity and functional activity in broiler chicken blood after using of animal origin immunomodulators in conditions of pre-slaughter stress. The authors determined the relative amount of T- and B-lymphocytes and their individual populations in the reaction of spontaneous rоsetting with the sheep erythrocytes in blood. Besides, the differentiated count of rоsetting lymphocytes with the various degree of functional activity was conducted. The spleen extract (70% alcohol solution in volume of 1.4 ml per chicken) was added to the diet of broiler chicken of experimental groups by aerosol method. This extract was obtained with/ without ultrasound application. 70% alcohol solution in the same volume and using the same method was added to the diet of broiler chicken of the control group five days before slaughter. The authors have not established probable increase of T-lymphocytes general quantity in broiler chicken blood in both experimental groups. It is shown that pre-slaughter stress in broiler chicken caused by weaning has immuno-suppressive effect on T- and B-lymphocytes in blood, which is accompanied by their quantity and functional activity decrease. T- and B-lymphocytes amount and functional activity of T- and B-cell immunity was stimulated after adding immunomodulators of natural origin to broiler chicken diet. Spleen extract polyamines as immunomodulators and antistressors most effectively influenced on some of cell immunity indices before slaughter – it is necessary to note the increase in T-helper lymphocytes in the broiler chickens blood caused by lymphocytes with medium (6–10) – by 18% (Р < 0.05) and high density receptors (М) – by 35% (Р < 0.05) compared to the control. It is shown that decrease of T-lymphocytes quantity in broiler chicken blood is caused by lymphocytes with law (3–5) – by 22% (Р < 0.01) and high (M) – by 11% (Р < 0.05) density receptors with increase of medium density receptors (6–10) – by 15% (Р < 0.05) compared to the control. The results obtained in the experiment can be used in the investigations of cell immunity indices of farm animals in order to increase the organism resistance and to correct their pre-slaughter stress. 

scholarly journals ВПЛИВ ІМУНОМОДУЛЯТОРІВ НА ПОКАЗНИКИ КЛІТИННОГО ІМУНІТЕТУ ЛОШАТ ВЕРХОВИХ ПОРІД

2016 ◽  
Vol 18 (3(71)) ◽  
pp. 45-49
Author(s):  
I.P. Кrytsia
Keyword(s):  
T Cells ◽  
Immune System ◽  
T Lymphocytes ◽  
Functional Activity ◽  
T Helper Cells ◽  
Hematological Parameters ◽  
Immune Reactivity ◽  
T Helper ◽  
Physiological Level ◽  
Helper Cells

To maintain a body at sufficient physiological level the effective functioning of the immune system, which determines the resistance and immune reactivity of animals, is necessary. In our studies in newborn foals indicators of cellular immunity were mature. During the studying of foals of all ages were established the reduction of hematological parameters in animals months of age.The use of immunomodulators prevents the immunodeficiency in animals. Immunomodulators introduction for animals normalizes T–immune system, in particular, increases the number of leucocytes in the blood, lymphocytes of certain populations, especially teofilin–resistant subpopulation of T–helper cells, increases the functional activity of lymphocytes.Under influence of ribotan revealed a trend to the increasing of T–lymphocytes by 0.2 – 1.2% (0.4 – 2.3%), respectively in Purebred Saddle and Ukrainian Saddle breeds. Results of the content of T–helper and T–suppressor cells in foals blood after ribotan administration showed that the use of immunomodulators not only increases the number of T–helper cells, but restores the ratio T–h / T–s, which returned to the optimal rate (1.9). Analyzing the functional status of T–lymphocytes during the application of immunomodulators was found the probable increase of the number of activated T–lymphocytes in Purebred Saddle foals more than 2–fold (P <0.01) and trend to increase of these cells in Ukrainian Saddle foals. In relation to thermostable T–lymphocytes, was note that the trend to the most optimal level of these cells installed in foals after administration of ribotan (values within 3 – 4%). The increasing in number of thermostable T–cells more than 4% indicates an increase power of suppressor T–cells population, indicating the inhibition of T–helper cells, and therefore the production of antibodies. Thus, the use of ribotan in dose of 1 ml / animal for three days leads to an increasing in 1.4 – 4.5% of the number of leukocytes in the blood of experimental group of foals compared with control animals. Under influence of ribotan in the blood of foals increases cell (number of T–lymphocytes in 0.4 – 2.3%) and functional activity (T–active lymphocytes in 2.3 times; P < 0.05) T–immune system. Under influence of cycloferon in the blood of foals increases the functional activity of T–immune system (the number of T–active lymphocytes in 16.7 – 25%; P < 0.05). 

scholarly journals PECULIARITIES OF POST-FIRM REHABILITATION PIGLES DEPENDING ON THE SEASON OF BIRTH

2021 ◽  
pp. 122-128
Author(s):  
N. V. Efanova ◽  
L. M. Osina ◽  
S. V. Batalova
Keyword(s):  
Immune System ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Immune Status ◽  
Season Of Birth ◽  
B Lymphopoiesis ◽  
T Helper ◽  
Low Level ◽  
Different Seasons ◽  
Activated T Lymphocytes

The features of the rehabilitation of the immune system of piglets after weaning were studied in connection with different seasons of farrowing. To fulfill this goal, 4 groups were formed from the offspring of sows of the SM-1 breed of Novosibirsk selection. The 1st group included piglets from winter farrowings, and the 2nd, 3rd and 4th groups, respectively, young animals born in spring, summer and autumn. Each group consisted of 30 animals. Studies of indicators of the immune status were carried out before weaning and 30 days after weaning. Weaning of piglets from sows with their subsequent regrouping in the educational farm «Tulinskoye» is carried out at the age of 60 days. The research results have shown that the state of the immune status of 2- and 3-month-old pigs is seasonal. Piglets born in autumn show the best indicators of leukopoiesis, T-, B-lymphopoiesis, the formation of functionally mature T-lymphocytes, activated T-lymphocytes and antitelogenesis. The second position in terms of the intensity of leukopoiesis, antitelogenesis and B-lymphopoiesis is occupied by young animals born in summer. Piglets raised in winter differ from their peers in a low level of IgM synthesis, while piglets born in spring have the lowest total numbers of T-lymphocytes, T-helper inductors, T-killer suppressors, B-lymphocytes and IgG. A month after weaning, animals, regardless of the season of birth, showed a statistically significant decrease in T-lymphopoiesis. The activity of the T-link of the immune system decreased most significantly in young animals obtained in spring. However, in contrast to their peers, youngsters born in the spring showed a statistically significant increase in blood IgM and IgG concentrations. In piglets born in winter, summer and autumn, the activity of IgM synthesis, on the contrary, decreased, while the formation of IgG remained at the same level.

scholarly journals FEATURES OF THE PHENOTYPE OF REGULATORY T CELLS IN EARLY AND ADVANCED RHEUMATOID ARTHRITIS

2018 ◽  
Vol 56 (4) ◽  
pp. 423-428
Author(s):  
A. S. Avdeeva ◽  
Yu. P. Rubtsov ◽  
T. V. Popkova ◽  
D. T. Dyikanov ◽  
A. P. Aleksankin ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cells ◽  
T Lymphocytes ◽  
Regulatory T Cells ◽  
B Lymphocytes ◽  
Functional Activity ◽  
Lower Percentage ◽  
Advanced Stage ◽  
Early Ra ◽  
Cd4 Lymphocyte

Objective: to analyze the levels of CD3+, CD3+CD4+, CD3+CD8+, and CD3-CD56+ T lymphocytes, FoxP3+ regulatory T cells (Treg), and CD19+ B lymphocytes in patients with early and advanced rheumatoid arthritis (RA). Subjects and methods. The investigation enrolled 45 patients previously untreated with methotrexate (MTX-naive) who had early RA and 15 patients who had advanced RA. Immunofluorescence staining and multicolor flow cytometry assays were used to estimate the percentage and absolute (abs) counts of CD3+, CD3+CD4+, CD3+CD8+, CD3-CD16+CD56+, CD19+, Treg(FoxP3+CD25+; surface CD152+; intracellular CD152+; FoxP3+CD127; CD25+CD127-; FoxP3+ICOS+; FoxP3+CD154+; and FoxP3+CD274+. Results and discussion. The patients with early RA were found to have a lower percentage of FoxP3+CD25+ cells and lower percentages and abs counts of FoxP3+ ICOS+ cells, FoxP3+CD154+ cells, and FoxP3+ CD274+ T cells than healthy donors (p<0.05 in all cases). The patients with advanced RA were also recorded to have a lower percentage of FoxP3+CD25+ cells and lower percentages and abs contents of FoxP3+ ICOS+ cells, FoxP3+CD154+ cells, and FoxP3+ CD274+ T cells (p><0.05 in all cases). The patients with advanced RA compared to those with early RA had a higher content of CD4+ lymphocytes (50.7 [44.4; 53.1] and 45.0 [38.0; 49.2]) and lower percentages of CD25+CD127- T lymphocytes (5.0 [4.0; 5.7] and 6.5 [5.1; 7.9] respectively; p><0.05 in all cases). Conclusion. Patients with RA (with the early or advanced stage of the disease) show a decrease in both the counts and functional activity of Treg. The patients with advanced RA compared with those with early RA showed an increase in CD4+ lymphocyte counts and a decrease in CD25+CD127- cell levels, which suggests that there are more pronounced impairments in Treg homeostasis in advanced RA. Keywords: early rheumatoid arthritis; T lymphocytes; B lymphocytes; regulatory T cells><0.05 in all cases). The patients with advanced RA were also recorded to have a lower percentage of FoxP3+CD25+ cells and lower percentages and abs contents of FoxP3+ ICOS+ cells, FoxP3+CD154+ cells, and FoxP3+ CD274+ T cells (p<0.05 in all cases). The patients with advanced RA compared to those with early RA had a higher content of CD4+ lymphocytes (50.7 [44.4; 53.1] and 45.0 [38.0; 49.2]) and lower percentages of CD25+CD127- T lymphocytes (5.0 [4.0; 5.7] and 6.5 [5.1; 7.9] respectively; p><0.05 in all cases). Conclusion. Patients with RA (with the early or advanced stage of the disease) show a decrease in both the counts and functional activity of Treg. The patients with advanced RA compared with those with early RA showed an increase in CD4+ lymphocyte counts and a decrease in CD25+CD127- cell levels, which suggests that there are more pronounced impairments in Treg homeostasis in advanced RA. Keywords: early rheumatoid arthritis; T lymphocytes; B lymphocytes; regulatory T cells><0.05 in all cases). The patients with advanced RA compared to those with early RA had a higher content of CD4+ lymphocytes (50.7 [44.4; 53.1] and 45.0 [38.0; 49.2]) and lower percentages of CD25+CD127- T lymphocytes (5.0 [4.0; 5.7] and 6.5 [5.1; 7.9] respectively; p<0.05 in all cases). Conclusion. Patients with RA (with the early or advanced stage of the disease) show a decrease in both the counts and functional activity of Treg. The patients with advanced RA compared with those with early RA showed an increase in CD4+ lymphocyte counts and a decrease in CD25+CD127- cell levels, which suggests that there are more pronounced impairments in Treg homeostasis in advanced RA. Keywords: early rheumatoid arthritis; T lymphocytes; B lymphocytes; regulatory T cells><0.05 in all cases). Conclusion. Patients with RA (with the early or advanced stage of the disease) show a decrease in both the counts and functional activity of Treg. The patients with advanced RA compared with those with early RA showed an increase in CD4+ lymphocyte counts and a decrease in CD25+CD127- cell levels, which suggests that there are more pronounced impairments in Treghomeostasis in advanced RA. 

scholarly journals High levels ofbcl-2 protein in circulating t lymphocytes, but not b lymphocytes, of patients with systemic lupus erythematosus

1994 ◽  
Vol 37 (10) ◽  
pp. 1423-1430 ◽  
Author(s):  
Martin Aringer ◽  
Winfried Wintersberger ◽  
Carl W. Steiner ◽  
Hans Kiener ◽  
Elisabeth Presterl ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Lupus Erythematosus ◽  
Systemic Lupus

scholarly journals T lymphocyte-dependent B lymphocyte proliferative response to antigen. I Genetic restriction of the stimulation of B lymphocyte proliferation.

1981 ◽  
Vol 153 (4) ◽  
pp. 871-882 ◽  
Author(s):  
H Y Tse ◽  
J J Mond ◽  
W E Paul
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
T Lymphocyte ◽  
Lymphocyte Proliferation ◽  
B Lymphocyte ◽  
Antigen Specificity ◽  
Apparent Lack ◽  
The Face ◽  
Stimulation Of

For the purpose of examining more closely the interaction between T and B lymphocytes, we have developed an in vitro T lymphocyte-dependent B lymphocyte proliferation assay. Proliferation of B lymphocytes in response to antigen was found to depend on the presence of primed T lymphocytes; the B lymphocytes could be derived from nonprimed animals. It appears that these B cells were nonspecifically recruited to proliferate. This nonspecific recruitment, however, was found to be Ir-gene restricted in that B lymphocytes from B10.S mice, which are genetic nonresponders to the polymer Glu60-Ala30-Tyr10 (GAT), could not be stimulated by GAT-primed (responder X nonresponder) F1 T cells. The apparent lack of antigen specificity in the face of Ir gene-restricted T-B interaction may have important implications in our understanding of the recognition unit(s) on T lymphocytes.

scholarly journals DC-SIGN on B Lymphocytes Is Required For Transmission of HIV-1 to T Lymphocytes

2006 ◽  
Vol 2 (7) ◽  
pp. e70 ◽  
Author(s):  
Giovanna Rappocciolo ◽  
Paolo Piazza ◽  
Craig L Fuller ◽  
Todd A Reinhart ◽  
Simon C Watkins ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
Hiv 1

scholarly journals Correction: DC-SIGN on B Lymphocytes Is Required for Transmission of HIV-1 to T Lymphocytes

2006 ◽  
Vol 2 (8) ◽  
pp. e88 ◽  
Author(s):  
Giovanna Rappocciolo ◽  
Paolo Piazza ◽  
Craig L. Fuller ◽  
Todd A. Reinhart ◽  
Simon C. Watkins ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
Hiv 1

scholarly journals Activity of the T- and B-system of the cell immunity of animals under conditions of oxidation stress and effects of the liposomal drug

2017 ◽  
Vol 7 (4) ◽  
pp. 536-541 ◽  
Author(s):  
M. Khariv ◽  
B. Gutyj ◽  
N. Ohorodnyuk ◽  
O. Vishchur ◽  
I. Khariv ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Cellular Immunity ◽  
Relative Number ◽  
The Body ◽  
T And B Lymphocytes ◽  
Oxidation Stress ◽  
Liposomal Preparation ◽  
Experimental Group

The results of research on the influence of the elaborated complex immunotropic drug containing butafosfan, interferon, thistle and fat-soluble vitamins A, D3, E in the form of a liposomal emulsion on the activity of T- and B-cell chains of immunity in rats under the conditions of action on the body of oxidative stress are presented. It has been established that the introduction of 50% tetrachloromethane into the rats of the first and second experimental groups, with a dose of 0.25 ml per 100 g of body weight, causes oxidative stress in them which negatively affects the cellular immunity and functional activity of immunocompetent blood cells. Immunosuppressive effects of oxidative stress were manifested by a decrease in the blood of rats in the first and second experimental groups of the number of T- and B-lymphocytes and their regulatory subpopulations mainly on the 2nd and 5th day of the study. At the same time, in the blood of rats of the first experimental group in all research periods a decrease in the relative number of common, active and theophylline-resistant T-lymphocytes, as well as B-lymphocytes was observed with a noticeable increase in the number of their undifferentiated forms. At the same time, the obtained data suggest the positive effect of butafosfan, interferon, thistle and vitamins A, D3, E in the liposomal preparation on the relative amount of T- and B-lymphocytes and on the redistribution of avidity in the direction of strengthening the receptor field of plasma membranes immunocompetent cells. It was found that the normalization on the 2nd day of blood level in the second experimental group of common T-lymphocytes occurred due to the secondary forms of the blood and active T-lymphocytes by changes in the number of low-avid forms. In addition to the indicators characterizing the cellular immunity of rats, the components of the liposomal preparation showed regulatory influence on the humoral link of the immune response. In particular, on the 10th day of research in blood of rats of the second experimental group a tendency towards an increase in the relative number of B-lymphocytes and an increase in the number of cells with low and medium density of receptors was found, which, under the conditions of oxidative stress, indicates an increase in the body's ability to actively synthesize protective antibodies

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