scholarly journals Free and bound forms of sialic acid in blood plasma of patients with chronic lymphocytic leukemia

2015 ◽  
Vol 6 (2) ◽  
pp. 108-112 ◽  
Author(s):  
O. V. Netronina
Keyword(s):  
Sialic Acid ◽  
Chronic Lymphocytic Leukemia ◽  
Blood Plasma ◽  
Sialic Acids ◽  
Lymphocytic Leukemia ◽  
General Level ◽  
Control Group ◽  
Free Sialic Acid ◽  
In Blood Plasma

The level of free and bound forms of sialic acids (SА) was investigated in chronic lymphocytic leukemia (CLL) and at different stages of receiving chemotherapy. Determination of sialic acid in blood plasma of patients with CLL before treatment were carried out on the first day and 2 months after taking chemotherapy drugs for combination schemes. Hematologically healthy donors represented the control group. Determination of SA was conducted by thiobarbituric method using trichloroacetic acid for distribution of total sialic acids onto free, protein bound sialic acid, and oligo bound sialic acid. Levelof free sialic acid in serum in chronic lymphocytic leukemia was equal to 43.2% compared to the total number of plasma. On the first day of chemotherapy on background of the general level of oligo bound sialic acids we observed increase in free sialic acid by 24.2% compared to patients not receiving treatment. The level of sialic acids increased 2.6 times compared to norm and featured no significant changes at different stages of treatment. The concentration of sialic acids bound to proteins at 73.4% was lower compared to the control group. After receiving chemotherapy on the first day there was a decline of this indicator to 56.2% compared to groups of patients before treatment. Two months after the treatment the level of all parameters under study returned back to the values obtained at the start of treatment. This data can serve as an additional efficiency criterion of the chemotherapy.

scholarly journals Individual features of the pharmacokinetics of fludarabin phosphate in the treatment of patients with chronic lymphocytic leukemia

2021 ◽  
pp. 67-77
Author(s):  
G. G. Rodionov ◽  
I. I. Shantyr ◽  
V. B. Vasilyuk ◽  
E. A. Kolobova ◽  
E. V. Svetkina ◽  
...  
Keyword(s):  
Chronic Lymphocytic Leukemia ◽  
Quantitative Determination ◽  
Blood Plasma ◽  
B Cell ◽  
Individual Variability ◽  
Lymphocytic Leukemia ◽  
Pharmacokinetic Parameters ◽  
Cell Chronic Lymphocytic Leukemia ◽  
In Blood Plasma

Fludarabine is a purine antimetabolite with a pronounced immunosuppressive effect. The inhibitory effect of fludarabine depends on its concentration in blood plasma. In addition, the phenotypic characteristics of patients affect the pharmacokinetic and pharmacodynamic profile of the drug, which necessitates a personalized approach to the dosage regimen. The chromatography-mass spectrometric method for the quantitative determination of 2-fluorine in blood plasma was developed for studying the individual parameters of pharmacokinetics of the international non-proprietary name (INN) fludarabine in patients with B-cell chronic lymphocytic leukemia during the standard course. Such method for the quantitative determination of 2-fluorine in blood plasma was developed and validated in accordance with international requirements. Significant individual variability of the main pharmacokinetic parameters in patients with B-cell chronic lymphocytic leukemia with a single oral administration of the drug with INN fludarabine at a dose of 40 mg/m2 was established, so the coefficient of variability Cmax was 42 %, Tmax — 92 %, AUC0-t — 45 %, Kel — 23 %, T1/2 — 26 %. It should be noted that there is a high interindividual variability of fludarabine, for example, 24 hours after taking the study drug, the maximum and minimum plasma concentrations of the fludarabine metabolite 2-fluoro-ara-A in different in patients with B-cell chronic lymphocytic leukemia differed 9 times. Individual variability of pharmacokinetic parameters characterizing absorption (Cmax/AUC0-t) and total clearance of the active metabolite of fludarabine is statistically significantly associated with a combination of gender and anthropometric factors.

scholarly journals The copper content in the blood plasma of children with different types of short stature

2020 ◽  
Vol 73 (2) ◽  
pp. 266-271
Author(s):  
Tamila V. Sorokman ◽  
Snezhana V. Sokolnyk ◽  
Oleksandra-Maria V. Popelyuk ◽  
Tetyana O. Bezruk ◽  
Olena V. Makarova
Keyword(s):  
Growth Hormone ◽  
Blood Plasma ◽  
Short Stature ◽  
Copper Content ◽  
Copper Level ◽  
Control Group ◽  
Different Types ◽  
Comprehensive Survey ◽  
In Blood Plasma

The aim: to investigate the copper content in blood serum of children with different short stature types. Materials and methods: In the first stage, a comprehensive survey of 258 school children aged 7-11 was carried out in Chernivtsi to assess the prevalence of short stature children of school-age. In the second stage, a comparative study of 42 children aged 3 to 15 years with different types of short stature was conducted. Research: anthropometry, determination of growth hormone, TSH, IGF-1, and copper level in blood plasma. Results: The largest growth rate in children with somatotropic insufficiency, the syndrome of a biologically inactive growth hormone ranged from 4.1 cm/year to 1.6 cm/year, the ones with a family constitutional low-growth – from 5.7 cm/year to 3.3 cm/year. In 57 cases, that makes 1% of children with short stature, the blood plasma level of copper made less and rated 0.89 ± 0.04 μg / ml, which is believed to be lower (pу0,001) the average copper in children of the control group – 1.07 ± 0.02 μg / ml. Indices of copper in blood plasma in children with somatotropic insufficiency were the lowest and averaged 0.75 ± 0.03 μg / l, p<0.05. Conclusions: The lack of copper in the blood plasma is observed in most children with short stature, which indicates the need for appropriate correction.

COMPARATIVE ACTION OF VARIOUS OESTROGENIC COMPOUNDS ON MOUSE VAGINAL SIALIC ACIDS (II)

1969 ◽  
Vol 62 (4) ◽  
pp. 663-670 ◽  
Author(s):  
Lars Carlborg
Keyword(s):  
Sialic Acid ◽  
Response Curve ◽  
Sialic Acids ◽  
Clear Cut ◽  
Suitable Parameter ◽  
Sufficient Degree ◽  
Comparative Action ◽  
Relative Potencies ◽  
Good Agreement

ABSTRACT Oestrogens administered in lower doses than necessary to induce full cornification of the mouse vagina induce mucification. It was shown previously that the degree of mucification could be estimated by quantitative determination of sialic acids. A suitable parameter for oestrogen assay was the measurement of vaginal sialic acid concentration which exhibited a clear cut dose response curve. Eleven assays of various oestrogens were performed with this method. Their estimated relative potencies were in good agreement with other routine oestrogen assays. A statistically sufficient degree of precision was found. The sensitivity was of the same order, or slightly higher, than the Allen-Doisy test.

Two SPRi biosensors for the determination of cathepsin S in blood plasma

Talanta ◽  
2021 ◽  
Vol 225 ◽  
pp. 121900
Author(s):  
Lukasz Oldak ◽  
Anna Sankiewicz ◽  
Beata Żelazowska-Rutkowska ◽  
Bogdan Cylwik ◽  
Zenon Lukaszewski ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Cathepsin S ◽  
In Blood Plasma

scholarly journals DETERMINATION OF TRYPSIN INHIBITOR IN BLOOD PLASMA

1953 ◽  
Vol 204 (1) ◽  
pp. 147-152 ◽  
Author(s):  
Shirley F. McCann ◽  
M. Laskowski
Keyword(s):  
Blood Plasma ◽  
Trypsin Inhibitor ◽  
In Blood Plasma

The convenient UPLS method for the determination of Ceftiofur in blood plasma

2020 ◽  
Author(s):  
Artem V. Baklykov ◽  
Konstantin A. Chistiakov ◽  
Dmitry S. Kopchuk ◽  
Grigory V. Zyryanov ◽  
Gennady L. Rusinov ◽  
...  
Keyword(s):  
Blood Plasma ◽  
In Blood Plasma

scholarly journals Determination of organoarsenic species in blood plasma by HPLC-ICP MS

1999 ◽  
Vol 13 (3) ◽  
pp. 183-187 ◽  
Author(s):  
Les Ebdon ◽  
Andrew Fisher ◽  
Norman B. Roberts ◽  
Magdi Yaqoob
Keyword(s):  
Blood Plasma ◽  
Icp Ms ◽  
In Blood Plasma
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