scholarly journals L-ascorbic acid-2-phosphate and Fibrin Substrate Enhances the Chondrogenic Differentiation of Human Adipose-derived Stem Cells

2021 ◽  
Vol 8 (2) ◽  
pp. 310-317
Author(s):  
Imam Rosadi ◽  
Karina Karina ◽  
Viol Dhea Kharisma ◽  
Arif Nur Muhammad Ansori
Keyword(s):  
Stem Cells ◽  
Ascorbic Acid ◽  
Chondrogenic Differentiation ◽  
Adipose Derived Stem Cells

Electromagnetic fields enhance chondrogenesis of human adipose-derived stem cells in a chondrogenic microenvironment in vitro

2013 ◽  
Vol 114 (5) ◽  
pp. 647-655 ◽  
Author(s):  
Chung-Hwan Chen ◽  
Yi-Shan Lin ◽  
Yin-Chih Fu ◽  
Chih-Kuang Wang ◽  
Shun-Cheng Wu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Electromagnetic Field ◽  
Cell Viability ◽  
Chondrogenic Differentiation ◽  
Collagen Type ◽  
Cartilage Tissue ◽  
Pulse Electromagnetic Field ◽  
Type I ◽  
Adipose Derived Stem Cells ◽  
Pemf Treatment

We tested the hypothesis that electromagnetic field (EMF) stimulation enhances chondrogenesis in human adipose-derived stem cells (ADSCs) in a chondrogenic microenvironment. A two-dimensional hyaluronan (HA)-coated well (2D-HA) and a three-dimensional pellet culture system (3D-pellet) were used as chondrogenic microenvironments. The ADSCs were cultured in 2D-HA or 3D-pellet, and then treated with clinical-use pulse electromagnetic field (PEMF) or the innovative single-pulse electromagnetic field (SPEMF) stimulation. The cytotoxicity, cell viability, and chondrogenic and osteogenic differentiations were analyzed after PEMF or SPEMF treatment. The modules of PEMF and SPEMF stimulations used in this study did not cause cytotoxicity or alter cell viability in ADSCs. Both PEMF and SPEMF enhanced the chondrogenic gene expression (SOX-9, collagen type II, and aggrecan) of ADSCs cultured in 2D-HA and 3D-pellet. The expressions of bone matrix genes (osteocalcin and collagen type I) of ADSCs were not changed after SPEMF treatment in 2D-HA and 3D-pellet; however, they were enhanced by PEMF treatment. Both PEMF and SPEMF increased the cartilaginous matrix (sulfated glycosaminoglycan) deposition of ADSCs. However, PEMF treatment also increased mineralization of ADSCs, but SPEMF treatment did not. Both PEMF and SPEMF enhanced chondrogenic differentiation of ADSCs cultured in a chondrogenic microenvironment. SPEMF treatment enhanced ADSC chondrogenesis, but not osteogenesis, when the cells were cultured in a chondrogenic microenvironment. However, PEMF enhanced both osteogenesis and chondrogenesis under the same conditions. Thus the combination of a chondrogenic microenvironment with SPEMF stimulation can promote chondrogenic differentiation of ADSCs and may be applicable to articular cartilage tissue engineering.

scholarly journals Norepinephrine induces migration but inhibits chondrogenic differentiation of syonovial adipose-derived stem cells

2018 ◽  
Vol 26 ◽  
pp. S136-S137
Author(s):  
K. El Bagdadi ◽  
F. Zaucke ◽  
A. Meurer ◽  
R.H. Straub ◽  
Z. Jenei-Lanzl
Keyword(s):  
Stem Cells ◽  
Chondrogenic Differentiation ◽  
Adipose Derived Stem Cells

scholarly journals The Influence of Cell Culture Density on the Cytotoxicity of Adipose-Derived Stem Cells Induced by L-Ascorbic Acid-2-Phosphate

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yuan-Kun Wu ◽  
Yuan-Kun Tu ◽  
Jiashing Yu ◽  
Nai-Chen Cheng
Keyword(s):  
Stem Cells ◽  
Ascorbic Acid ◽  
Cell Sheet ◽  
Metabolic Stress ◽  
Seeding Density ◽  
Cell Contact ◽  
High Dose ◽  
Adipose Derived Stem Cells ◽  
Enhanced Expression ◽  
High Concentrations

AbstractAscorbic acid-2-phosphate (A2-P) is an oxidation-resistant derivative of ascorbic acid that has been widely employed in culturing adipose-derived stem cells (ASCs) for faster expansion and cell sheet formation. While high dose ascorbic acid is known to induce cellular apoptosis via metabolic stress and genotoxic effects, potential cytotoxic effects of A2-P at high concentrations has not been explored. In this study, the relationship between ASC seeding density and A2-P-induced cytotoxicity was investigated. Spheroid-derived ASCs with smaller cellular dimensions were generated to investigate the effect of cell-cell contact on the resistance to A2-P-induced cytotoxicity. Decreased viability of ASC, fibroblast, and spheroid-derived ASC was noted at higher A2-P concentration, and it could be reverted with high seeding density. Compared to control ASCs, spheroid-derived ASCs seeded at the same density exhibited decreased viability in the A2-P-supplemented medium. The expression of antioxidant enzymes (catalase, SOD1, and SOD2) was enhanced in ASCs at higher seeding densities. However, their enhanced expression in spheroid-derived ASCs was less evident. Furthermore, we found that co-administration of catalase or N-acetylcysteine nullified the observed cytotoxicity. Collectively, A2-P can induce ASC cytotoxicity at higher concentrations, which can be prevented by seeding ASCs at high density or co-administration of another antioxidant.

scholarly journals Differential effects of BMP-2 and TGF-β1 on chondrogenic differentiation of adipose derived stem cells

2007 ◽  
Vol 40 (6) ◽  
pp. 809-823 ◽  
Author(s):  
A. T. Mehlhorn ◽  
P. Niemeyer ◽  
K. Kaschte ◽  
L. Muller ◽  
G. Finkenzeller ◽  
...  
Keyword(s):  
Stem Cells ◽  
Chondrogenic Differentiation ◽  
Adipose Derived Stem Cells ◽  
Differential Effects ◽  
Tgf Β1

Effects of scaffold geometry on chondrogenic differentiation of adipose-derived stem cells

2020 ◽  
Vol 110 ◽  
pp. 110733 ◽  
Author(s):  
Kai-Chiang Yang ◽  
Ing-Ho Chen ◽  
Ya-Ting Yang ◽  
Jong-Kai Hsiao ◽  
Chen-Chie Wang
Keyword(s):  
Stem Cells ◽  
Chondrogenic Differentiation ◽  
Adipose Derived Stem Cells

A quick potency assay for osteogenic and chondrogenic differentiation of adipose derived stem cells

2014 ◽  
Vol 31 ◽  
pp. S11
Author(s):  
Eleni Oberbauer ◽  
Florian Hildner ◽  
Ara Hacobian ◽  
Susanne Wolbank ◽  
Carolin Steffenhagen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Chondrogenic Differentiation ◽  
Adipose Derived Stem Cells ◽  
Potency Assay

ERRATUM IN: Influence of different growth factors on chondrogenic differentiation of adipose-derived stem cells in polyurethane-fibrin composites”

2013 ◽  
Vol 36 (1) ◽  
pp. 73-73
Author(s):  
Katrin Froelich ◽  
Lydia E. Setiawan ◽  
Antje Technau ◽  
Mario Ramos Tirado ◽  
Stephan Hackenberg ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Chondrogenic Differentiation ◽  
Adipose Derived Stem Cells
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