scholarly journals Impact of TGF-β family-related growth factors on chondrogenic differentiation of adipose-derived stem cells isolated from lipoaspirates and infrapatellar fat pads of osteoarthritic patients

2018 ◽  
Vol 35 ◽  
pp. 209-224 ◽  
Author(s):  
E López-Ruiz ◽  
◽  
G Jiménez ◽  
W Kwiatkowski ◽  
E Montañez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Chondrogenic Differentiation ◽  
Adipose Derived Stem Cells ◽  
Fat Pads

ERRATUM IN: Influence of different growth factors on chondrogenic differentiation of adipose-derived stem cells in polyurethane-fibrin composites”

2013 ◽  
Vol 36 (1) ◽  
pp. 73-73
Author(s):  
Katrin Froelich ◽  
Lydia E. Setiawan ◽  
Antje Technau ◽  
Mario Ramos Tirado ◽  
Stephan Hackenberg ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Chondrogenic Differentiation ◽  
Adipose Derived Stem Cells

Influence of Different Growth Factors on Chondrogenic Differentiation of Adipose-Derived Stem Cells in Polyurethane-Fibrin Composites

2012 ◽  
Vol 35 (12) ◽  
pp. 1047-1060 ◽  
Author(s):  
Katrin Froelich ◽  
Lydia E. Setiawan ◽  
Antje Technau ◽  
Mario Ramos Tirado ◽  
Stephan Hackenberg ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Chondrogenic Differentiation ◽  
Vocal Cord Palsy ◽  
Cartilage Defects ◽  
Positive Staining ◽  
Adipose Derived Stem Cells ◽  
General Ability ◽  
Collagen Ii ◽  
Acid Glycosaminoglycans

Introduction Chondrogenic differentiation of adipose-derived stem cells (ASCs) has proven to be feasible. To compensate for laryngeal palsy or cartilage defects after surgery or trauma using tissue engineering, a formable and stable scaffold material is mandatory. Methods ASCs were seeded in fibrin-polyurethane scaffolds and cultured in chondrogenic differentiation medium adding the growth factors TGF-□1, TGF-□3, and BMP-2 for up to 35 days. Results Histological examination showed acid glycosaminoglycans in the extracellular matrix in all groups. Immunofluorescence presented positive staining for collagen II, aggrecan, and SOX-9 in the TGF-□1–, TGF-□3–, and BMP-2-group. With Real-time PCR analyses, chondrogenic differentiation became apparent by the expression of the specific genes COL2A1 (collagen II), AGC 1 (aggrecan), and SOX-9, whereas collagen II expression was low in all groups compared to bone marrow-derived stem cells (BMSC) due to reduced chondrogenic ability. Conclusions These findings demonstrate the general ability of ASCs to differentiate into matrix-producing chondrocytes in fibrin-polyurethane scaffolds. However, further experiments are necessary to enhance this chondrogenic potential of ASCs seeded in fibrin-polyurethane scaffolds in order to produce a suitable regeneration method for treating cartilage defects or an implantable medialization material for vocal cord palsy.

Influence of different growth factors on chondrogenic differentiation of adipose-derived stem cells in polyurethane-fibrin composites

2012 ◽  
Vol 35 (12) ◽  
pp. 1047-1060 ◽  
Author(s):  
Katrin Froelich ◽  
Lydia E. Setiawan ◽  
Antje Technau ◽  
Mario Ramos Tirado ◽  
Stephan Hackenberg ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Chondrogenic Differentiation ◽  
Adipose Derived Stem Cells

Electromagnetic fields enhance chondrogenesis of human adipose-derived stem cells in a chondrogenic microenvironment in vitro

2013 ◽  
Vol 114 (5) ◽  
pp. 647-655 ◽  
Author(s):  
Chung-Hwan Chen ◽  
Yi-Shan Lin ◽  
Yin-Chih Fu ◽  
Chih-Kuang Wang ◽  
Shun-Cheng Wu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Electromagnetic Field ◽  
Cell Viability ◽  
Chondrogenic Differentiation ◽  
Collagen Type ◽  
Cartilage Tissue ◽  
Pulse Electromagnetic Field ◽  
Type I ◽  
Adipose Derived Stem Cells ◽  
Pemf Treatment

We tested the hypothesis that electromagnetic field (EMF) stimulation enhances chondrogenesis in human adipose-derived stem cells (ADSCs) in a chondrogenic microenvironment. A two-dimensional hyaluronan (HA)-coated well (2D-HA) and a three-dimensional pellet culture system (3D-pellet) were used as chondrogenic microenvironments. The ADSCs were cultured in 2D-HA or 3D-pellet, and then treated with clinical-use pulse electromagnetic field (PEMF) or the innovative single-pulse electromagnetic field (SPEMF) stimulation. The cytotoxicity, cell viability, and chondrogenic and osteogenic differentiations were analyzed after PEMF or SPEMF treatment. The modules of PEMF and SPEMF stimulations used in this study did not cause cytotoxicity or alter cell viability in ADSCs. Both PEMF and SPEMF enhanced the chondrogenic gene expression (SOX-9, collagen type II, and aggrecan) of ADSCs cultured in 2D-HA and 3D-pellet. The expressions of bone matrix genes (osteocalcin and collagen type I) of ADSCs were not changed after SPEMF treatment in 2D-HA and 3D-pellet; however, they were enhanced by PEMF treatment. Both PEMF and SPEMF increased the cartilaginous matrix (sulfated glycosaminoglycan) deposition of ADSCs. However, PEMF treatment also increased mineralization of ADSCs, but SPEMF treatment did not. Both PEMF and SPEMF enhanced chondrogenic differentiation of ADSCs cultured in a chondrogenic microenvironment. SPEMF treatment enhanced ADSC chondrogenesis, but not osteogenesis, when the cells were cultured in a chondrogenic microenvironment. However, PEMF enhanced both osteogenesis and chondrogenesis under the same conditions. Thus the combination of a chondrogenic microenvironment with SPEMF stimulation can promote chondrogenic differentiation of ADSCs and may be applicable to articular cartilage tissue engineering.

Effects of Three-Dimensional Culture and Growth Factors on the Chondrogenic Differentiation of Murine Embryonic Stem Cells

Stem Cells ◽  
2006 ◽  
Vol 24 (2) ◽  
pp. 284-291 ◽  
Author(s):  
Nathaniel S. Hwang ◽  
Myoung Sook Kim ◽  
Somponnat Sampattavanich ◽  
Jin Hyen Baek ◽  
Zijun Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Embryonic Stem Cells ◽  
Chondrogenic Differentiation ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Murine Embryonic Stem Cells ◽  
Three Dimensional Culture

scholarly journals Norepinephrine induces migration but inhibits chondrogenic differentiation of syonovial adipose-derived stem cells

2018 ◽  
Vol 26 ◽  
pp. S136-S137
Author(s):  
K. El Bagdadi ◽  
F. Zaucke ◽  
A. Meurer ◽  
R.H. Straub ◽  
Z. Jenei-Lanzl
Keyword(s):  
Stem Cells ◽  
Chondrogenic Differentiation ◽  
Adipose Derived Stem Cells

scholarly journals Differential effects of BMP-2 and TGF-β1 on chondrogenic differentiation of adipose derived stem cells

2007 ◽  
Vol 40 (6) ◽  
pp. 809-823 ◽  
Author(s):  
A. T. Mehlhorn ◽  
P. Niemeyer ◽  
K. Kaschte ◽  
L. Muller ◽  
G. Finkenzeller ◽  
...  
Keyword(s):  
Stem Cells ◽  
Chondrogenic Differentiation ◽  
Adipose Derived Stem Cells ◽  
Differential Effects ◽  
Tgf Β1

Effects of scaffold geometry on chondrogenic differentiation of adipose-derived stem cells

2020 ◽  
Vol 110 ◽  
pp. 110733 ◽  
Author(s):  
Kai-Chiang Yang ◽  
Ing-Ho Chen ◽  
Ya-Ting Yang ◽  
Jong-Kai Hsiao ◽  
Chen-Chie Wang
Keyword(s):  
Stem Cells ◽  
Chondrogenic Differentiation ◽  
Adipose Derived Stem Cells

A quick potency assay for osteogenic and chondrogenic differentiation of adipose derived stem cells

2014 ◽  
Vol 31 ◽  
pp. S11
Author(s):  
Eleni Oberbauer ◽  
Florian Hildner ◽  
Ara Hacobian ◽  
Susanne Wolbank ◽  
Carolin Steffenhagen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Chondrogenic Differentiation ◽  
Adipose Derived Stem Cells ◽  
Potency Assay
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