scholarly journals Role of AP-1 transcriptional factor in development of oxidative and nitrosative stress in periodontal tissues during systemic inflammatory response

2019 ◽  
Vol 91 (1) ◽  
pp. 80-85 ◽  
Author(s):  
A. M. Yelins’ka ◽  
◽  
O. Ye. Akimov ◽  
V. O. Kostenko ◽  
◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Nitrosative Stress ◽  
Transcriptional Factor ◽  
Systemic Inflammatory Response ◽  
Oxidative And Nitrosative Stress ◽  
Periodontal Tissues

scholarly journals QUERCETIN LIMITS THE DEVELOPMENT OF OXIDATIVE-NITROSATIVE STRESS IN PERIODONTAL TISSUES IN DIFFERENT MODELS SIMULATING SYSTEMIC INFLAMMATORY RESPONSE

2019 ◽  
Vol 19 (4) ◽  
pp. 83-87
Author(s):  
A.M. Yelins’ka ◽  
S.M. Nazarenko ◽  
V.O. Kostenko
Keyword(s):  
Inflammatory Response ◽  
Nadph Oxidase ◽  
Nitrosative Stress ◽  
Soft Tissues ◽  
Salmonella Typhi ◽  
No Synthase ◽  
Systemic Inflammatory Response ◽  
Water Soluble ◽  
Soluble Form ◽  
Periodontal Tissues

The study was carried out to investigate the effect produced by water-soluble form of quercetin (corvitin) on the indices of the development of oxidative-nitrosative stress in periodontal soft tissues of rats subjected to systemic inflammatory response. This condition was simulated by using two models: one was induced by the Salmonella typhi lipopolysaccharide (LPS) administration (in a dose of 0.4 μg / kg of body wt three times through the 1st week and once a week for the next 7 weeks), as well as on the 14th day after a moderate craniocerebral injury (CCI). Applying corvitin in a dose of 500 μg / kg (10 μg / kg recalculated as quercetin) every third day starting from the 30th day of the experiment with the use of pyrogenalum reduced the production of superoxide anion radical (.О) by NADPH-dependent electron transport chains (endoplasmic reticulum and NO-synthase) by 18.1%, but did not considerably affect the mitochondrial chain. .О production by leukocyte NADPH-oxidase was by 16.7% lower. The total NO synthase (NOS) activity in periodontal tissues decreased by 40.6%, and the content of peroxynitrite ions was inferior to the relevant result of the group received pyrogenalum by 13.9%. Administration of corvitin in a dose of 500 mg/kg for 7 days after CCI modeling reduced the (.О) production by NADPH-dependent chains by 20.9%, and by mitochondria by 31.7% on the 14th day of post-traumatic period. .О production by leukocyte NADPH-oxidase was by 35.8% lower. NOS activity in periodontal tissues decreased by 45.8%, the content of peroxynitrite ions was inferior to the relevant value in the group with modeled CCI by 25.7%. This suggests the conclusion that applying water-soluble form of quercetin in conditions of systemic inflammatory response limits the signs of oxidative-nitrosative stress in periodontal soft tissues of rats.

Possible role of increased oxidant stress in multiple organ failure after systemic inflammatory response syndrome

2003 ◽  
Vol 31 (4) ◽  
pp. 1048-1052 ◽  
Author(s):  
Takeshi Motoyama ◽  
Kazufumi Okamoto ◽  
Ichirou Kukita ◽  
Masamichi Hamaguchi ◽  
Yoshihiro Kinoshita ◽  
...  
Keyword(s):  
Systemic Inflammatory Response Syndrome ◽  
Inflammatory Response ◽  
Multiple Organ Failure ◽  
Organ Failure ◽  
Oxidant Stress ◽  
Multiple Organ ◽  
Systemic Inflammatory Response

scholarly journals The role of oxidative and nitrosative stress in the pathology of osteoarthritis: Novel candidate biomarkers for quantification of degenerative changes in the knee joint

2018 ◽  
Vol 10 (2) ◽  
Author(s):  
Alexander Franz ◽  
Laura Joseph ◽  
Constantin Mayer ◽  
Jan-Frieder Harmsen ◽  
Holger Schrumpf ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Synovial Fluid ◽  
Knee Joint ◽  
Human Serum ◽  
Synovial Membrane ◽  
Nitrosative Stress ◽  
Control Groups ◽  
Oxidative And Nitrosative Stress ◽  
And Oxidative Stress

Osteoarthritis (OA) is the most frequently diagnosed joint disorder worldwide with increasing prevalence and crucial impact on the quality of life of affected patients through chronic pain, decreasing mobility and invalidity. Although some risk factors, such as age, obesity and previous joint injury are well established, the exact pathogenesis of OA on a cellular and molecular level remains less understood. Today, the role of nitrosative and oxidative stress has not been investigated conclusively in the pathogenesis of OA yet. Therefore, the objective of this study was to identify biological substances for oxidative and nitrosative stress, which mirror the degenerative processes in an osteoarthritic joint. 69 patients suffering from a diagnosed knee pain participated in this study. Based on the orthopedic diagnosis, patients were classified into an osteoarthritis group (OAG, n=24) or in one of two control groups (meniscopathy, CG1, n=11; anterior cruciate ligament rupture, CG2, n=34). Independently from the study protocol, all patients underwent an invasive surgical intervention which was used to collect samples from the synovial membrane, synovial fluid and human serum. Synovial biopsies were analyzed histopathologically for synovitis (Krenn-Score) and immunohistochemically for detection of end products of oxidative (8-isoprostane F2α) and nitrosative (3-nitrotyrosine) stress. Additionally, the fluid samples were analyzed for 8-isoprostane F2α and 3-nitrotyrosine by competitive ELISA method. The analyzation of inflammation in synovial biopsies revealed a slight synovitis in all three investigated groups. Detectable concentrations of 3-nitrotyrosine were reported in all three investigated groups without showing any significant differences between the synovial biopsies, fluid or human serum. In contrast, significant increased concentrations of 8-isoprostane F2α were detected in OAG compared to both control groups. Furthermore, our data showed a significant correlation between the histopathological synovitis and oxidative stress in OAG (r=0.728, P<0.01). There were no significant differences between the concentrations of 8-isoprostane F2α in synovial fluid and human serum. The findings of the current study support the hypothesis that oxidative and nitrosative stress are components of the multi-factory pathophysiological formation of OA. It seems reasonable that an inflammatory process in the synovial membrane triggers the generation of oxidative and nitrosative acting substances which can lead to a further degradation of the articular cartilage. Based on correlations between the observed degree of inflammation and investigated biomarkers, especially 8-isoprostane F2α seems to be a novel candidate biomarker for OA. However, due to the finding that also both control groups showed increased concentrations of selected biomarkers, future studies have to validate the diagnostic potential of these biomarkers in OA and in related conditions of the knee joint.

The Role of IL-27 in the Systemic Inflammatory Response That Accompanies Preterm Labour

Inflammation ◽  
2021 ◽  
Author(s):  
Youwen Mei ◽  
Yuxin Ran ◽  
Zheng Liu ◽  
Yunqian Zhou ◽  
Jie He ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Preterm Labour ◽  
Systemic Inflammatory Response
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