scholarly journals The analysis of the genetic polymorphism of Chlorella vulgaris Beyer. culture growing in the presence of sodium selenite, zinc sulfate and chromium chloride

Algologia ◽  
2021 ◽  
Vol 31 (2) ◽  
pp. 113-125
Author(s):  
O.I. Bodnar ◽  
◽  
I.O. Andreev ◽  
M.Z. Prokopiak ◽  
N.M. Drobyk ◽  
...  
Keyword(s):  
Trace Elements ◽  
Genetic Polymorphism ◽  
Chlorella Vulgaris ◽  
Pcr Analysis ◽  
Algal Culture ◽  
Algae Cultivation ◽  
Inter Simple Sequence Repeats ◽  
The Media ◽  
Metabolic Reactions ◽  
And Control

The use of microalgae for the economic needs and the commercial goals determines the areas of the scientific researches that will make it possible to increase their productivity. It is also important to direct the metabolism of the algae to the activating of certain synthetic processes in order to obtain the desired compounds. The metals and non-metals, entering into the cell, have a high biochemical activity. These elements modify the metabolic reactions in general and the metabolic reactions related to the functioning of the genome of microalgae cells. Aim. The aim was to study the genetic polymorphism of Chlorella vulgaris under the action of such trace elements as selenium, zinc and chromium in order to optimize the methods of algae cultivation and the obtaining of the beneficial compounds. Methods. The hydrobiological methods of algae cultivation, DNA isolation method by Rogers S. and Bendich A. (1985), PCR-analysis with ISSR (inter simple sequence repeats)- and IRAP-markers (inter-retransposon amplified polymorphism) have been used. Results. For all samples of C. vulgaris 109 DNA-fragments were obtained and 42 of them were polymorphic (38.5%). Jacquard distances (DJ) between the samples of C. vulgaris culture (cultures are grown on the media with different elements compositions and control (standard conditions) were 0.232 (only selenite), 0.206 (selenite and zinc) and 0.300 (selenite and chromium). Conclusions. Probably the genetic modifications of C. vulgaris cells are caused by the additional introduction of the microelements into the culture medium. The genetic polymorphism of the algae grown on media with various trace elements and their combinations was like the genetic polymorphism of the unicellular green algae grown in the natural conditions. It indicates the absence of significant genotoxic effects of the trace elements and high metabolic and genetic plasticity of algal culture.

THE HORMONAL COMPOSITION OF MILK IN PRODUCTIVE ANIMALS AND ITS SAFETY FOR HUMANS

2020 ◽  
Vol 1 (3) ◽  
pp. 313-321
Author(s):  
P.A. Popov ◽  
◽  
V.S. Babunova ◽  
Keyword(s):  
Trace Elements ◽  
Growth Factor ◽  
Dairy Products ◽  
The Body ◽  
The Other ◽  
Raw Material ◽  
Important Food ◽  
The Past ◽  
Highly Sensitive ◽  
And Control

Hormones are an integral part of milk and throughout lactation, the content of certain hormones is unstable. Hormones regulate the process of starting lactation of animals, the lactation process itself, and also the other functions of the body. Milk is of great importance for the growth of young animals and the formation of immunity. Milk is a special product in the diet and is an important food and raw material for the production of dairy products for people. It contains a large amount of protein, fat, carbohydrates, vitamins and trace elements in biologically available form. But at the same time, over the past few years, more and more evidence has emerged that hormones in dairy products can impact on human health. Thus, some estrogens and insulin-like growth factor IGF-1 are involved in the initiation and provocation of breast, prostate and endometrial tumors. That’s why, it is necessary to normalize and control the content of certain hormones in milk with highly sensitive methods.

scholarly journals PO-055 Changes of trace elements in skeletal muscle and serum of rats after exercise-induced injury

2018 ◽  
Vol 1 (3) ◽  
Author(s):  
Jingyun Liu ◽  
Qun Zuo
Keyword(s):  
Skeletal Muscle ◽  
Trace Elements ◽  
Nitric Acid ◽  
Vena Cava ◽  
The Body ◽  
Emission Spectrometry ◽  
Control Group ◽  
Exercise Induced ◽  
And Control ◽  
Cu And Zn

Objective This study is to investigate the changes of trace elements (Cu, Fe, Zn, Se, Mg) in serum and skeletal muscle of rats after skeletal muscle injury induced by downhill running, and to find out the change regularity of trace elements in the body after exercise injury. To provide experimental basis for how to use trace elements supplements reasonably. Methods Fifty-four healthy male Sprague-Dawley rats aged 8 weeks were randomly divided into two groups: control group (C, N=6) and exercise group (E, N=48, include: 0 h group, 6 h group, 12 h group, 24 h group, 48 h group, 72 h group, 1- week group and 2- week group). The rats in exercise groups run down a 16°incline at 16m/min for 90 minutes. At the end of the exercise, the rats were killed at 0 h, 6 h, 12 h, 24 h, 48 h, 72 h, 1 week and 2 weeks, respectively. The serum was got from the inferior vena cava blood and diluted by 1% nitric acid. The muscle was got from the right side of the rat's sural which were digested by concentrated nitric acid and 30% hydrogen peroxide in 75℃water bath for 20mins. The content of trace elements in muscle and serum were measured by inductively coupled plasma atomic emission spectrometry (ICP-MS). All the data are analyzed and processed by SPSS22.0 statistical software. Results (1) The contents of trace elements in serum showed: Cu, Zn, Mg, Se decreased immediately after exercise, but the Cu still increased to reach a peak at 24h after decreasing, and after 2 weeks the content of Cu was slightly lower than pre-exercise level. However, the content of Zn did not elevate again, it continued declined to the lowest at 24h which was significantly lower than control group (P < 0.05). And after 2 weeks, Zn did not return to the pre-exercise level. The changes of Mg, Se in serum was not statistically significant. There is no difference between 0h and control groups in content of Fe, after that Fe decreased continually and appeared the least value at 24h, the differences between immediate group and control group were statistically significant (P < 0.05). Fe returned to the pre-exercise level after 2 weeks. (2) The contents of trace elements in muscle showed: Most of trace elements increased to the maximum level at 6 h, after that Mg, Fe, Cu decreased to the lowest value at 72 h which were significant lower than 0h group or 6h group (P < 0. 05). ALL the trace elements were lower than pre-exercise level. There was no statistical difference in the content of Se in muscle. Conclusions (1) The different changes of trace elements in skeletal muscle and serum after exercise injury may be due to the redistribution of trace elements caused by the body adaptability. (2) The most obviously changes of trace element in serum and muscle are Cu and Zn. Both of them did not return to the pre-exercise level after 2 weeks, it suggests that the supplement include Cu and Zn may play an important role in recovering after exercise-induced injury.

scholarly journals World local government control and reference value for Vietnam

2021 ◽  
Vol 63 (4) ◽  
pp. 29-33
Author(s):  
Dang Phuc Vu ◽  
◽  
Thi Thanh Nga Nguyen ◽  
Keyword(s):  
Local Government ◽  
Local Governments ◽  
Central Government ◽  
Reference Value ◽  
Social Institutions ◽  
Local Power ◽  
Government Control ◽  
The People ◽  
The Media ◽  
And Control

Control of local governments in countries around the world is very diverse,but mainly divided into two categories: 1) control of state agencies (central government control, court control, control of local power representations); 2) control of social institutions including political party control over local government, control of organizations and public associations, control of the media, and control of the people. The paper focuses on analysing the controlling local governments in some countries, thereby giving reference values for Vietnam.

scholarly journals Injection of ligand-free gold and silver nanoparticles into murine embryos does not impact pre-implantation development

2014 ◽  
Vol 5 ◽  
pp. 677-688 ◽  
Author(s):  
Ulrike Taylor ◽  
Wiebke Garrels ◽  
Annette Barchanski ◽  
Svea Peterson ◽  
Laszlo Sajti ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Embryo Development ◽  
Internal Control ◽  
Blastocyst Stage ◽  
Pcr Analysis ◽  
Gold And Silver Nanoparticles ◽  
Free Gold ◽  
Ligand Free ◽  
Murine Embryos ◽  
And Control

Intended exposure to gold and silver nanoparticles has increased exponentially over the last decade and will continue to rise due to their use in biomedical applications. In particular, reprotoxicological aspects of these particles still need to be addressed so that the potential impacts of this development on human health can be reliably estimated. Therefore, in this study the toxicity of gold and silver nanoparticles on mammalian preimplantation development was assessed by injecting nanoparticles into one blastomere of murine 2 cell-embryos, while the sister blastomere served as an internal control. After treatment, embryos were cultured and embryo development up to the blastocyst stage was assessed. Development rates did not differ between microinjected and control groups (gold nanoparticles: 67.3%, silver nanoparticles: 61.5%, sham: 66.2%, handling control: 79.4%). Real-time PCR analysis of six developmentally important genes (BAX, BCL2L2, TP53, OCT4, NANOG, DNMT3A) did not reveal an influence on gene expression in blastocysts. Contrary to silver nanoparticles, exposure to comparable Ag+-ion concentrations resulted in an immediate arrest of embryo development. In conclusion, the results do not indicate any detrimental effect of colloidal gold or silver nanoparticles on the development of murine embryos.

scholarly journals Study of Chlorella vulgaris sedimentation process

2018 ◽  
Vol 240 ◽  
pp. 05023 ◽  
Author(s):  
Agnieszka Patyna ◽  
Małgorzata Płaczek ◽  
Stanisław Witczak
Keyword(s):  
Chlorella Vulgaris ◽  
Dry Mass ◽  
Separation Process ◽  
Cultivation Condition ◽  
Microalgal Biomass ◽  
Algae Biomass ◽  
Algae Cultivation ◽  
Sedimentation Process ◽  
Microalgae Harvesting

The paper reports the results of Chlorella vulgaris sedimentation process including description of cultivation condition of microalgal biomass. The process of algae cultivation was carried out in photobioreactor comprising systems of carbon dioxide supply, mixing and artificial LED illumination. The growth of microalgae was determined alternatively in three ways by measuring the amount of dry mass over time, counting the cells and measurement of optical density by use of a spectrophotometer. Algae biomass with different concentration was subjected to the separation process by gravity. This led to the determination of the characteristic of sedimentation process for different concentrations and cell sizes. The experimental results indicate that sedimentation process offers a tool with a potential application for microalgae harvesting.

Twentieth-Century Influences on the Development in Britain of Services for Child and Adolescent Psychiatry

1991 ◽  
Vol 159 (1) ◽  
pp. 53-68 ◽  
Author(s):  
Christopher J. Wardle
Keyword(s):  
Rapid Development ◽  
Child And Adolescent Psychiatry ◽  
Therapeutic Interventions ◽  
Teaching Hospitals ◽  
The Past ◽  
Child Guidance ◽  
Wide Range ◽  
The Media ◽  
Post War ◽  
And Control

Modern comprehensive multidisciplinary mental-health services for children and adolescents have four origins: psychology from 1890, psychoanalysis from 1906, the child-guidance movement from 1920, and the children's departments of psychiatric teaching hospitals from 1930. Post-war changes in society and reform, especially the NHS Act 1946, contributed to rapid development of services and an increasingly wide range of sophisticated therapeutic interventions; professional and interdisciplinary associations and trans-Atlantic exchange were also influential. In the last three decades a succession of official inquiries, reports, legislation and reorganisations have had a damaging effect. Children and their services have been prey tocauses célèbres,fashion and the exaggerated fads and foibles of the media and politicians; they have thrived best when society and their carers were tolerant, and loving, sought good qualities to augment, not evil to exorcise, and succeeded in balancing structure and control with flexibility and freedom to grow. Planners should review the past before acting.

scholarly journals Canada’s Invisible Nationality Policy: Creating Ethnicity, Managing Populations, Imagining a Nation

2014 ◽  
Vol 24 (2) ◽  
pp. 263-310
Author(s):  
Evgeny Efremkin
Keyword(s):  
Critical Discourse Analysis ◽  
Racial Differences ◽  
Race And Ethnicity ◽  
Social Order ◽  
Economic Status ◽  
Public Officials ◽  
Nationality Policy ◽  
Ethnic Populations ◽  
The Media ◽  
And Control

This article explores the process of national identity construction at Canadian borders in the 1930s. Based on the examination of customs declaration forms (transatlantic ship manifests) of arriving passengers at the port of Quebec in the first half of the 1930s, as well as Royal Canadian Mountain Police (RCMP) files, I suggest that the Canadian bureaucracy, informed by social Darwinist views of race and ethnicity, and the Anglophone bourgeois concern over modernization brought upon by the forces of industrialization and urbanization, developed an elaborate system of categorization of Canada’s population according to prescribed criteria of ethnicity, nationality, and race. Utilizing critical discourse analysis, I argue that in efforts to “know” and control its growing population, the Canadian state developed a rigid, albeit an invisible nationality policy. Although there was never an official nationality policy in place in Canada in the 1930s, public officials, the media, and security agencies not only operated with an acute awareness of national and ethno-racial differences in the society, but also worked to reinforce such divisions in attempts to maintain the social order and the cultural, social, and economic status quo. In this work, I imply that border officials were directly responsible for constructing specific representations of Canada’s ethnic populations, all within the context of an impending need to control the population of a rapidly modernizing society, where the Canadian community had to be made “knowable,” familiar, and recognizable in the official discourse.

Sign in / Sign up

Export Citation Format

Share Document