Investigation of the catabolism of 7-hydroxycoumarin in Pseudomonas mandelii 7HK4 bacteria

2021 ◽  
Author(s):  
◽  
Arūnas Krikštaponis
Keyword(s):  
Pseudomonas Mandelii

Exemplar Abstract for Pseudomonas mandelii Verhille et al. 1999.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Pseudomonas Mandelii

Molecular cloning and biochemical characterization of a NAD-dependent sorbitol dehydrogenase from cold-adapted Pseudomonas mandelii

2021 ◽  
Author(s):  
Quynh DangThu ◽  
Thu-Thuy Nguyen ◽  
Sei-Heon Jang ◽  
ChangWoo Lee
Keyword(s):  
Biochemical Characterization ◽  
Size Exclusion ◽  
Sorbitol Dehydrogenase ◽  
High Catalytic Activity ◽  
Active Site Residues ◽  
Tertiary Structures ◽  
Cold Adapted ◽  
Pseudomonas Mandelii ◽  
Catalytic Active Site ◽  
Exclusion Chromatography

Abstract Sugar alcohols (polyols) have important roles as nutrients, anti-freezing agents, and scavengers of free radicals in cold-adapted bacteria, but the characteristics of polyol dehydrogenases in cold-adapted bacteria remain largely unknown. In this study, based on the observation that a cold-adapted bacterium Pseudomonas mandelii JR-1 predominantly utilized D-sorbitol as its carbon source, among the four polyols examined (D-galactitol, D-mannitol, D-sorbitol, or D-xylitol), we cloned and characterized a sorbitol dehydrogenase (SDH, EC 1.1.1.14) belonging to the short-chain dehydrogenase/reductase family from this bacterium (the SDH hereafter referred to as PmSDH). PmSDH contained Asn111, Ser140, Tyr153, and Lys157 as catalytic active site residues and existed as a ∼67 kDa dimer in size-exclusion chromatography. PmSDH converted D-sorbitol to D-fructose using NAD+ as a coenzyme and, vice versa, D-fructose to D-sorbitol using NADH as a coenzyme. PmSDH maintained its conformational flexibility, secondary and tertiary structures, and thermal stability at 4–25°C. At 40°C, PmSDH was rapidly denatured. These results indicate that PmSDH, which has a flexible structure and a high catalytic activity at colder temperatures, is well-suited to sorbitol utilization in the cold-adapted bacterium P. mandelii JR-1.

scholarly journals Psychrotrophic Pseudomonas mandelii CBS-1 produces high levels of poly-β-hydroxybutyrate

SpringerPlus ◽  
2013 ◽  
Vol 2 (1) ◽  
pp. 335 ◽  
Author(s):  
Rongpeng Li ◽  
Yuji Jiang ◽  
Xinfeng Wang ◽  
Jingjing Yang ◽  
Yuan Gao ◽  
...  
Keyword(s):  
Pseudomonas Mandelii

Structure of the O-polysaccharide of Pseudomonas mandelii CYar1 containing 3,6-dideoxy-4-C-[(S)-1-hydroxyethyl]-d-xylo-hexose (yersiniose A)

2013 ◽  
Vol 381 ◽  
pp. 138-141 ◽  
Author(s):  
Anna N. Kondakova ◽  
Marina S. Drutskaya ◽  
Alexander S. Shashkov ◽  
Sergei A. Nedospasov ◽  
Vladimir N. Akimov ◽  
...  
Keyword(s):  
Pseudomonas Mandelii

scholarly journals Complete Genome Sequence of Pseudomonas sp. Strain MM213, an Isolate from a Brookside in Bielefeld, Germany

2022 ◽  
Author(s):  
Bart Verwaaijen ◽  
Özgülen Cevahir ◽  
Fabian Hitz ◽  
Jacqueline Römmich ◽  
Donat Wulf
Keyword(s):  
Genome Size ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Gc Content ◽  
Pseudomonas Sp ◽  
Protein Coding ◽  
Coding Sequences ◽  
Content Type ◽  
Pseudomonas Mandelii

Here, we report the complete genome sequence of Pseudomonas sp. strain MM213 of the Pseudomonas mandelii group, which was isolated from a brookside in Bielefeld, Germany. The genome size is 6,746,355 bp, with a GC content of 59.4% and 6,145 predicted protein-coding sequences.

scholarly journals Effect of pH and Temperature on Denitrification Gene Expression and Activity in Pseudomonas mandelii

2009 ◽  
Vol 75 (12) ◽  
pp. 3903-3911 ◽  
Author(s):  
Saleema Saleh-Lakha ◽  
Kelly E. Shannon ◽  
Sherri L. Henderson ◽  
Claudia Goyer ◽  
Jack T. Trevors ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fold Increase ◽  
Low Ph ◽  
Effect Of Ph ◽  
Liquid Cultures ◽  
Ph Values ◽  
Reverse Transcription Pcr ◽  
Pseudomonas Mandelii ◽  
Denitrification Gene ◽  
Denitrification Activity

ABSTRACT Pseudomonas mandelii liquid cultures were studied to determine the effect of pH and temperature on denitrification gene expression, which was quantified by quantitative reverse transcription-PCR. Denitrification was measured by the accumulation of nitrous oxide (N2O) in the headspace in the presence of acetylene. Levels of gene expression of nirS and cnorB at pH 5 were 539-fold and 6,190-fold lower, respectively, than the levels of gene expression for cells grown at pH 6, 7, and 8 between 4 h and 8 h. Cumulative denitrification levels were 28 μmol, 63 μmol, and 22 μmol at pH 6, 7, and 8, respectively, at 8 h, whereas negligible denitrification was measured at pH 5. P. mandelii cells grown at 20°C and 30°C exhibited 9-fold and 94-fold increases in levels of cnorB expression between 0 h and 2 h, respectively, and an average 17-fold increase in levels of nirS gene expression. In contrast, induction of cnorB and nirS gene expression for P. mandelii cells grown at 10°C did not occur in the first 4 h. Levels of cumulative denitrification at 10 h were 6.6 μmol for P. mandelii cells grown at 10°C and 20°C and 30 μmol for cells grown at 30°C. Overall, levels of cnorB and nirS expression were relatively insensitive to pH values over the range of pH 6 to 8 but were substantially reduced at pH 5, whereas gene expression was sensitive to temperature, with induction and time to achieve maximum gene expression delayed as the temperature decreased from 30°C. Low pH and temperature negatively affected denitrification activity.

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