scholarly journals An Organic Solvent-Tolerant Alkaline Lipase from Cold-Adapted Pseudomonas mandelii: Cloning, Expression, and Characterization

2013 ◽  
Vol 77 (2) ◽  
pp. 320-323 ◽  
Author(s):  
Junsung KIM ◽  
Sei-Heon JANG ◽  
ChangWoo LEE
Keyword(s):  
Organic Solvent ◽  
Alkaline Lipase ◽  
Cold Adapted ◽  
Pseudomonas Mandelii ◽  
Organic Solvent Tolerant ◽  
Solvent Tolerant

scholarly journals Cold-adapted organic solvent tolerant alkalophilic family I.3 lipase from an Antarctic Pseudomonas

2016 ◽  
Vol 92 ◽  
pp. 1266-1276 ◽  
Author(s):  
Menega Ganasen ◽  
Norhayati Yaacob ◽  
Raja Noor Zaliha Raja Abd Rahman ◽  
Adam Thean Chor Leow ◽  
Mahiran Basri ◽  
...  
Keyword(s):  
Organic Solvent ◽  
Cold Adapted ◽  
Organic Solvent Tolerant ◽  
Solvent Tolerant

Transesterification of Waste Cooking Oil by an Organic Solvent-Tolerant Alkaline Lipase from Streptomyces sp. CS273

2013 ◽  
Vol 172 (3) ◽  
pp. 1377-1389 ◽  
Author(s):  
Poonam Mander ◽  
Hah-Young Yoo ◽  
Seung Wook Kim ◽  
Yun Hee Choi ◽  
Seung Sik Cho ◽  
...  
Keyword(s):  
Organic Solvent ◽  
Waste Cooking Oil ◽  
Alkaline Lipase ◽  
Streptomyces Sp ◽  
Cooking Oil ◽  
Organic Solvent Tolerant ◽  
Solvent Tolerant

Purification and characterization of a novel organic solvent-tolerant and cold-adapted lipase from Psychrobacter sp. ZY124

Extremophiles ◽  
2018 ◽  
Vol 22 (2) ◽  
pp. 287-300 ◽  
Author(s):  
Yue Zhang ◽  
Fangling Ji ◽  
Jingyun Wang ◽  
Zhongji Pu ◽  
Bo Jiang ◽  
...  
Keyword(s):  
Organic Solvent ◽  
Purification And Characterization ◽  
Cold Adapted ◽  
Organic Solvent Tolerant ◽  
Solvent Tolerant

scholarly journals New Recombinant Cold-Adapted and Organic Solvent Tolerant Lipase from Psychrophilic Pseudomonas sp. LSK25, Isolated from Signy Island Antarctica

2019 ◽  
Vol 20 (6) ◽  
pp. 1264 ◽  
Author(s):  
Leelatulasi Salwoom ◽  
Raja Raja Abd. Rahman ◽  
Abu Salleh ◽  
Fairolniza Mohd. Shariff ◽  
Peter Convey ◽  
...  
Keyword(s):  
Organic Solvent ◽  
Organic Solvents ◽  
Rice Bran Oil ◽  
Lipolytic Activity ◽  
Pseudomonas Sp ◽  
Lipase Gene ◽  
Cold Adapted ◽  
Organic Solvent Tolerant ◽  
Wide Range ◽  
Solvent Tolerant

In recent years, studies on psychrophilic lipases have become an emerging area of research in the field of enzymology. The study described here focuses on the cold-adapted organic solvent tolerant lipase strain Pseudomonas sp. LSK25 isolated from Signy Station, South Orkney Islands, maritime Antarctic. Strain LSK25 lipase was successfully cloned, sequenced, and over-expressed in an Escherichia coli system. Sequence analysis revealed that the lipase gene of Pseudomonas sp. LSK25 consists of 1432 bp, lacks an N-terminal signal peptide and encodes a mature protein consisting of 476 amino acids. The recombinant LSK25 lipase was purified by single-step purification using Ni-Sepharose affinity chromatography and had a molecular mass of approximately 65 kDa. The final recovery and purification fold were 44% and 1.3, respectively. The LSK25 lipase was optimally active at 30 °C and at pH 6. Stable lipolytic activity was reported between temperatures of 5–30 °C and at pH 6–8. A significant enhancement of lipolytic activity was observed in the presence of Ca2+ ions, the organic lipids of rice bran oil and coconut oil, a synthetic C12 ester and a wide range of water immiscible organic solvents. Overall, lipase strain LSK25 is a potentially desirable candidate for biotechnological application, due to its stability at low temperatures, across a range of pH and in organic solvents.

An organic solvent-tolerant alkaline lipase from Streptomyces sp. CS268 and its application in biodiesel production

2012 ◽  
Vol 17 (1) ◽  
pp. 67-75 ◽  
Author(s):  
Poonam Mander ◽  
Seung Sik Cho ◽  
Jaya Ram Simkhada ◽  
Yun Hee Choi ◽  
Da Jeong Park ◽  
...  
Keyword(s):  
Organic Solvent ◽  
Biodiesel Production ◽  
Alkaline Lipase ◽  
Streptomyces Sp ◽  
Organic Solvent Tolerant ◽  
Solvent Tolerant
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