scholarly journals Molecular Genetic Analysis of Chd3 and Polytene Chromosome Region 76B-D in Drosophila melanogaster

Genetics ◽  
2010 ◽  
Vol 185 (3) ◽  
pp. 811-822 ◽  
Author(s):  
Monica T. Cooper ◽  
Alexander W. Conant ◽  
James A. Kennison
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Analysis ◽  
Polytene Chromosome ◽  
Chromosome Region ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis

scholarly journals Toward a molecular genetic analysis of spermatogenesis in Drosophila melanogaster: characterization of male-sterile mutants generated by single P element mutagenesis.

Genetics ◽  
1993 ◽  
Vol 135 (2) ◽  
pp. 489-505 ◽  
Author(s):  
D H Castrillon ◽  
P Gönczy ◽  
S Alexander ◽  
R Rawson ◽  
C G Eberhart ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Analysis ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
P Element ◽  
Male Sterile ◽  
Germline Proliferation ◽  
Complementation Groups ◽  
Mutant Phenotypes

Abstract We describe 83 recessive autosomal male-sterile mutations, generated by single P element mutagenesis in Drosophila melanogaster. Each mutation has been localized to a lettered subdivision of the polytene map. Reversion analyses, as well as complementation tests using available chromosomal deficiencies, indicate that the insertions are responsible for the mutant phenotypes. These mutations represent 63 complementation groups, 58 of which are required for spermatogenesis. Phenotypes of the spermatogenesis mutants were analyzed by light microscopy. Mutations in 12 loci affect germline proliferation, spermatocyte growth, or meiosis. Mutations in 46 other loci disrupt differentiation and maturation of spermatids into motile sperm. This collection of male-sterile mutants provides the basis for a molecular genetic analysis of spermatogenesis.

Molecular genetic analysis of V-ATPase function in Drosophila melanogaster.

1997 ◽  
Vol 200 (2) ◽  
pp. 237-245 ◽  
Author(s):  
J A Dow ◽  
S A Davies ◽  
Y Guo ◽  
S Graham ◽  
M E Finbow ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Analysis ◽  
Plasma Membranes ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
P Element ◽  
Proton Pumps ◽  
Suitable Model ◽  
Animal Cells ◽  
General Utility

V-ATPases are phylogenetically widespread, highly conserved, multisubunit proton pumps. Originally characterised in endomembranes, they have been found to energise transport across plasma membranes in a range of animal cells and particularly in certain epithelia. While yeast is the model of choice for the rapid generation and identification of V-ATPase mutants, it does not allow their analysis in a plasma membrane context. For such purposes, Drosophila melanogaster is a uniquely suitable model. Accordingly, we have cloned and characterised genes encoding several V-ATPase subunits in D. melanogaster and, using P-element technology, we have succeeded in generating multiple new alleles. Reporter gene constructs reveal ubiquitous expression, but at particularly high levels in those epithelial thought to be energised by V-ATPases, and several of the alleles have lethal recessive phenotypes characterised by epithelial dysfunction. These results, while providing the first gene knockouts of V-ATPases in animals, also illustrate the general utility of D. melanogaster as a model for the genetic analysis of ion transport and its control in epithelia.

scholarly journals Molecular Genetic Analysis of the Nested Drosophila melanogaster Lamin C Gene

Genetics ◽  
2005 ◽  
Vol 171 (1) ◽  
pp. 185-196 ◽  
Author(s):  
Sandra R. Schulze ◽  
Beatrice Curio-Penny ◽  
Yuhong Li ◽  
Reza A. Imani ◽  
Lena Rydberg ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Analysis ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis

scholarly journals Repeated molecular genetic analysis in Brugada syndrome revealed a novel disease-associated large deletion in the SCN5A gene

2016 ◽  
Vol 2 (3) ◽  
pp. 261-264 ◽  
Author(s):  
Anders Krogh Broendberg ◽  
Lisbeth Noerum Pedersen ◽  
Jens Cosedis Nielsen ◽  
Henrik Kjaerulf Jensen
Keyword(s):  
Genetic Analysis ◽  
Brugada Syndrome ◽  
Molecular Genetic ◽  
Large Deletion ◽  
Molecular Genetic Analysis ◽  
Scn5a Gene
Sign in / Sign up

Export Citation Format

Share Document