Comparison of the KKU-DCIP Diagnostic Kit to the Standard Hemoglobin Electrophoresis Method for Detection of Hemoglobin E Carriers

2004 ◽  
Vol 10 (3) ◽  
pp. 189-190 ◽  
Author(s):  
SUPHAN SOOGARUN ◽  
SANONG SIRIMONGKOLSAKUL ◽  
BOONSRI MAHAKITTIKUL ◽  
VIROJ WIWANITKIT ◽  
PAWEENA PRADNIWAT ◽  
...  
Keyword(s):  
Hemoglobin E ◽  
Electrophoresis Method

Molecular and Hematological Characterization of Hemoglobin Hope/Hemoglobin E and Hemoglobin Hope/a-Thalassemia 2 in Thai Patients

2004 ◽  
Vol 10 (4) ◽  
pp. 215-220 ◽  
Author(s):  
SUNISA CHUNPANICH ◽  
SUPAN FUCHAROEN ◽  
KANOKWAN SANCHAISURIYA ◽  
GOONNAPA FUCHAROEN ◽  
KASIAN KAM-ITSARA
Keyword(s):  
Hemoglobin E

scholarly journals Denaturation of proteins and nucleic acids by thermal-gradient electrophoresis

1981 ◽  
Vol 197 (1) ◽  
pp. 105-109 ◽  
Author(s):  
D R Thatcher ◽  
B Hodson
Keyword(s):  
Conformational Changes ◽  
Polyacrylamide Gel Electrophoresis ◽  
Complex Mixture ◽  
Effect Of Temperature ◽  
Electrophoretic Method ◽  
Denatured State ◽  
Double Stranded Dna ◽  
Electrophoresis Method ◽  
Dna Restriction ◽  
Irreversible Unfolding

A polyacrylamide-gel-electrophoresis method has been developed that permits the analysis of conformational changes that occur during the thermal denaturation of macromolecules. A stable transverse temperature gradient was produced in an aluminium heating jacket clamped around a vertical polyacrylamide slab gel. After temperature equilibration, gels were loaded with either a layer of protein solution (20-200 micrograms/gel) or a solution of double-stranded DNA (20 micrograms/gel) and electrophoresis begun. At the end of the run the gels were stained and the effect of temperature on mobility observed. The technique proved informative both for the irreversible unfolding of proteins (Drosophila alcohol dehydrogenase and lactic acid dehydrogenase) and for a protein that was reversibly denatured by heat (beta-lactamase). In the latter case a clear transition between the native enzyme and a slower-migrating denatured state was observed. The patterns obtained were analogous to the type produced by the transverse-urea-gradient-electrophoretic method of Creighton [(1979) J. Mol. Biol. 129, 253-264]. The method also resolved a complex mixture of double-stranded-DNA restriction-digest fragments.

scholarly journals The Hemoglobin E Thalassemias

2012 ◽  
Vol 2 (8) ◽  
pp. a011734-a011734 ◽  
Author(s):  
S. Fucharoen ◽  
D. J. Weatherall
Keyword(s):  
Hemoglobin E

scholarly journals Development of a highly sensitive three-dimensional gel electrophoresis method for characterization of monoclonal protein heterogeneity

2013 ◽  
Vol 438 (2) ◽  
pp. 117-123 ◽  
Author(s):  
Keiichi Nakano ◽  
Shogo Tamura ◽  
Kohei Otuka ◽  
Noriyasu Niizeki ◽  
Masahiko Shigemura ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Three Dimensional ◽  
Monoclonal Protein ◽  
Highly Sensitive ◽  
Electrophoresis Method

Interaction of Hemoglobin E with Other Abnormal Hemoglobins

2011 ◽  
Vol 126 (4) ◽  
pp. 246-248 ◽  
Author(s):  
Eunice Sindhuvi Edison ◽  
Ramachandran V. Shaji ◽  
Mammen Chandy ◽  
Alok Srivastava
Keyword(s):  
Hemoglobin E
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