Canine spermatogonial stem cells transfected with GFP reporter gene transplanted in recipient teste mice

2014 ◽  
Author(s):  
Naira Caroline Godoy Pieri ◽  
Souza Aline Fernanda de ◽  
Ana Carolina Furlanetto Mancanares ◽  
Juliana Barbosa Casals ◽  
Kelly Cristine Roballo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Reporter Gene ◽  
Spermatogonial Stem Cells ◽  
Gfp Reporter ◽  
Gfp Reporter Gene

scholarly journals The Impact of GFP Reporter Gene Transduction and Expression on Metabolomics of Placental Mesenchymal Stem Cells Determined by UHPLC-Q/TOF-MS

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Jinfeng Yang ◽  
Nan Wang ◽  
Deying Chen ◽  
Jiong Yu ◽  
Qiaoling Pan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Reporter Gene ◽  
Fluorescent Protein ◽  
Medicine Research ◽  
Gfp Gene ◽  
Placental Mesenchymal Stem Cells ◽  
Gfp Reporter ◽  
Gfp Reporter Gene ◽  
The Impact

Introduction. Green fluorescent protein (GFP) is widely used as a reporter gene in regenerative medicine research to label and track stem cells. Here, we examined whether expressing GFP gene may impact the metabolism of human placental mesenchymal stem cells (hPMSCs). Methods. The GFP gene was transduced into hPMSCs using lentiviral-based infection to establish GFP+hPMSCs. A sensitive 13C/12C-dansyl labeling LC-MS method targeting the amine/phenol submetabolome was used for in-depth cell metabolome profiling. Results. A total of 1151 peak pairs or metabolites were detected from 12 LC-MS runs. Principal component analysis and partial least squares discriminant analysis showed poor separation, and the volcano plots demonstrated that most of the metabolites were not significantly changed when hPMSCs were tagged with GFP. Overall, 739 metabolites were positively or putatively identified. Only 11 metabolites showed significant changes. Metabolic pathway analyses indicated that three of the identified metabolites were involved in nine pathways. However, these metabolites are unlikely to have a large impact on the metabolic pathways due to their nonessential roles and limited hits in pathway analysis. Conclusion. This study indicated that the expression of ectopic GFP reporter gene did not significantly alter the metabolomics pathways covered by the amine/phenol submetabolome.

scholarly journals Insertion of a GFP Reporter Gene in Influenza Virus

2013 ◽  
Vol 29 (1) ◽  
pp. 15G.4.1-15G.4.16 ◽  
Author(s):  
Jasmine T. Perez ◽  
Adolfo García-Sastre ◽  
Balaji Manicassamy
Keyword(s):  
Influenza Virus ◽  
Reporter Gene ◽  
Gfp Reporter ◽  
Gfp Reporter Gene

Response Characteristics of Arsenic-Sensitive Bioreporters Expressing the gfp Reporter Gene

2005 ◽  
Vol 151 (3-4) ◽  
pp. 209-216 ◽  
Author(s):  
Mona Wells ◽  
Michael Gösch ◽  
Hauke Harms ◽  
Jan Roelof van der Meer
Keyword(s):  
Reporter Gene ◽  
Response Characteristics ◽  
Gfp Reporter ◽  
Gfp Reporter Gene

Generation of an NF-κB responsive, GFP-reporter gene mouse using a single copy HPRT gene-targeting system

2001 ◽  
Vol 120 (5) ◽  
pp. A303
Author(s):  
Scott T. Magness ◽  
Maria Pia Russo ◽  
David A. Brenner ◽  
Ryan B. Sartor ◽  
Christian Jobin
Keyword(s):  
Gene Targeting ◽  
Reporter Gene ◽  
Single Copy ◽  
Hprt Gene ◽  
Gfp Reporter ◽  
Gfp Reporter Gene

scholarly journals Cytoplasmically Retargeted HSV1-tk/GFP Reporter Gene Mutants for Optimization of Noninvasive Molecular-Genetic Imaging

Neoplasia ◽  
2003 ◽  
Vol 5 (3) ◽  
pp. 245-254 ◽  
Author(s):  
Vladimir Ponomarev ◽  
Michael Doubrovin ◽  
Inna Serganova ◽  
Tatiana Beresten ◽  
Jelena Vider ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Molecular Genetic ◽  
Gfp Reporter ◽  
Gfp Reporter Gene ◽  
Genetic Imaging

scholarly journals Bioreactor mixing efficiency modulates the activity of a prpoS::GFP reporter gene in E. coli

2009 ◽  
Vol 8 (1) ◽  
pp. 15 ◽  
Author(s):  
Frank Delvigne ◽  
Mathieu Boxus ◽  
Sophie Ingels ◽  
Philippe Thonart
Keyword(s):  
Reporter Gene ◽  
Mixing Efficiency ◽  
E Coli ◽  
Gfp Reporter ◽  
Gfp Reporter Gene
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