Tissue-specific expression of GFP reporter gene in germline driven by GATA-2 promoter and enhancers in zebrafish

2000 ◽  
Vol 45 (1) ◽  
pp. 31-34 ◽  
Author(s):  
Anming Meng ◽  
Shuo Lin
Keyword(s):  
Reporter Gene ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Gfp Reporter ◽  
Gfp Reporter Gene

Tissue-Specific Expression of Rat Light Neurofilament Promoter-Driven Reporter Gene in Transgenic Mice

1993 ◽  
Vol 192 (2) ◽  
pp. 465-470 ◽  
Author(s):  
M. Reeben ◽  
M. Halmekyto ◽  
L. Alhonen ◽  
R. Sinervirta ◽  
M. Saarma ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Reporter Gene ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression

scholarly journals Tissue-specific regulation of the mouse Pkhd1 (ARPKD) gene promoter

2014 ◽  
Vol 307 (3) ◽  
pp. F356-F368 ◽  
Author(s):  
Scott S. Williams ◽  
Patricia Cobo-Stark ◽  
Sachin Hajarnis ◽  
Karam Aboudehen ◽  
Xinli Shao ◽  
...  
Keyword(s):  
Binding Site ◽  
Reporter Gene ◽  
Lacz Reporter ◽  
Polycystic Kidney ◽  
Lacz Reporter Gene ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Endogenous Expression ◽  
Collecting Ducts

Autosomal recessive polycystic kidney disease, an inherited disorder characterized by the formation of cysts in renal collecting ducts and biliary dysgenesis, is caused by mutations of the polycystic kidney and hepatic disease 1 ( PKHD1) gene. Expression of PKHD1 is tissue specific and developmentally regulated. Here, we show that a 2.0-kb genomic fragment containing the proximal promoter of mouse Pkhd1 directs tissue-specific expression of a lacZ reporter gene in transgenic mice. LacZ is expressed in renal collecting ducts beginning during embryonic development but is not expressed in extrarenal tissues. The Pkhd1 promoter contains a binding site for the transcription factor hepatocyte nuclear factor (HNF)-1β, which is required for activity in transfected cells. Mutation of the HNF-1β-binding site abolishes the expression of the lacZ reporter gene in renal collecting ducts. Transgenes containing the 2.0-kb promoter and 2.7 kb of additional genomic sequence extending downstream to the second exon are expressed in the kidney, intrahepatic bile ducts, and male reproductive tract. This pattern overlaps with the endogenous expression of Pkhd1 and coincides with sites of expression of HNF-1β. We conclude that the proximal 2.0-kb promoter is sufficient for tissue-specific expression of Pkhd1 in renal collecting ducts in vivo and that HNF-1β is required for Pkhd1 promoter activity in collecting ducts. Additional genomic sequences located from exons 1-2 or elsewhere in the gene locus are required for expression in extrarenal tissues.

Tissue-specific expression patterns of nuclear receptors

2013 ◽  
Author(s):  
AL Bookout ◽  
Y Jeong ◽  
M Downes ◽  
RT Yu ◽  
RM Evans ◽  
...  
Keyword(s):  
Nuclear Receptors ◽  
Expression Patterns ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression
Sign in / Sign up

Export Citation Format

Share Document