Amino acid and glucose profiles of bovine oviduct epithelial cell secretions in response to 17[beta]-oestradiol and progesterone

2014 ◽  
Author(s):  
Constantine A Simintiras ◽  
Thozhukat Sathyapalan ◽  
Henry J Leese ◽  
Roger Sturmey
Keyword(s):  
Amino Acid ◽  
Epithelial Cell ◽  
Glucose Profiles ◽  
Oviduct Epithelial Cell

Characterization of bovine oviduct epithelial cell monolayers cultured under serum-free conditions

1995 ◽  
Vol 31 (9) ◽  
pp. 664-670 ◽  
Author(s):  
A. Van Langendonckt ◽  
A. Vansteenbrugge ◽  
C. Dessy-Doize ◽  
J. E. Flechon ◽  
G. Charpigny ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Serum Free ◽  
Cell Monolayers ◽  
Oviduct Epithelial Cell ◽  
Epithelial Cell Monolayers ◽  
Serum Free Conditions

Polarity of taurine transport in cultured renal epithelial cell lines: LLC-PK1 and MDCK

1993 ◽  
Vol 265 (1) ◽  
pp. F137-F145 ◽  
Author(s):  
D. P. Jones ◽  
L. A. Miller ◽  
R. W. Chesney
Keyword(s):  
Amino Acid ◽  
Epithelial Cell ◽  
Mdck Cell ◽  
Amino Acid Transport ◽  
Acid Transport ◽  
Renal Epithelial Cell ◽  
Amino Acid Transport System ◽  
Taurine Transport ◽  
Taurine Concentration ◽  
Epithelial Cell Lines

We characterized taurine transport in two continuous renal epithelial cell lines: LLC-PK1, derived from the proximal tubule of the pig, and the Madin-Darby canine kidney (MDCK), which was originated from the distal tubule of the dog. In the LLC-PK1 cell line, taurine transport is greatest at the apical surface of the cell, whereas in the MDCK cell line taurine transport is greatest at the basolateral surface. Both apical and basolateral surfaces of LLC-PK1 and MDCK cells exhibit an adaptive response to the extracellular taurine concentration (medium taurine concentration). Only the basolateral surface of the MDCK cell responded to hyperosmolality with increased taurine accumulation. This indicates differential control of the beta-amino acid transport system by substrate and external tonicity. The function of the beta-amino acid transport system may be different depending on the cell. In the LLC-PK1 cell, there is net transepithelial movement of taurine and changes in transporter activity in response to supply of substrate. In contrast, taurine accumulation by the MDCK cell appears to be a mechanism for adaptation to osmotic stress.

In vitro developmental potential of bovine zygotes in oviduct epithelial cell co-culture systems

1990 ◽  
Vol 33 (1) ◽  
pp. 223 ◽  
Author(s):  
J.E. Ellington ◽  
P.B. Farrell ◽  
E.W. Carney ◽  
M.E. Simkin ◽  
R.H. Foote
Keyword(s):  
Epithelial Cell ◽  
Developmental Potential ◽  
Culture Systems ◽  
Oviduct Epithelial Cell

scholarly journals Bovine Oviduct Epithelial Cell-Derived Culture Media and Exosomes Improve Mitochondrial Health by Restoring Metabolic Flux during Pre-Implantation Development

2020 ◽  
Vol 21 (20) ◽  
pp. 7589
Author(s):  
Tabinda Sidrat ◽  
Abdul Aziz Khan ◽  
Myeon-Don Joo ◽  
Yiran Wei ◽  
Kyeong-Lim Lee ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Embryonic Development ◽  
Metabolic Flux ◽  
Culture Media ◽  
Tca Cycle ◽  
Fine Tuning ◽  
The Tca Cycle ◽  
Oviduct Epithelial Cell

Oviduct flushing is enriched by a wide variety of nutrients that guide the 3–4 days journey of pre-implantation embryo through the oviduct as it develops into a competent blastocyst (BL). However, little is known about the specific requirement and role of these nutrients that orchestrate the early stages of embryonic development. In this study, we aimed to characterize the effect of in vitro-derived bovine oviduct epithelial cell (BOECs) secretion that mimics the in vivo oviduct micro-fluid like environment, which allows successful embryonic development. In this study, the addition of an in vitro derived BOECs-condition media (CM) and its isolated exosomes (Exo) significantly enhances the quality and development of BL, while the hatching ability of BLs was found to be high (48.8%) in the BOECs-Exo supplemented group. Surprisingly, BOECs-Exo have a dynamic effect on modulating the embryonic metabolism by restoring the pyruvate flux into TCA-cycle. Our analysis reveals that Exo treatment significantly upregulates the pyruvate dehydrogenase (PDH) and glutamate dehydrogenase (GLUD1) expression, required for metabolic fine-tuning of the TCA-cycle in the developing embryos. Exo treatment increases the influx into TCA-cycle by strongly suppressing the PDH and GLUD1 upstream inhibitors, i.e., PDK4 and SIRT4. Improvement of TCA-cycle function was further accompanied by higher metabolic activity of mitochondria in BOECs-CM and Exo in vitro embryos. Our study uncovered, for the first time, the possible mechanism of BOECs-derived secretion in re-establishing the TCA-cycle flux by the utilization of available nutrients and highlighted the importance of pyruvate in supporting bovine in vitro embryonic development.

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